Bond-selective transient phase imaging via sensing of the infrared photothermal effect
作者机构：Department of Biomedical EngineeringBoston UniversityBostonMA 02215USA Department of PhysicsZhejiang UniversityHangzhou 310028China National Laboratory on High Power Laser and PhysicsShanghai 201800China Key Laboratory of High Power Laser and PhysicsShanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China Department of BioengineeringUniversity of Illinois at Urbana-ChampaignChampaignIL 61801USA Department of Electrical and Computer EngineeringUniversity of Illinois at Urbana-ChampaignChampaignIL 61801USA Department of Electrical&Computer EngineeringBoston UniversityBostonMA 02215USA Photonics CenterBoston UniversityBostonMA 02215USA
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2019年第8卷第1期
页 面：70-81页
核心收录：
学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学]
基 金：supported by an R01 Grant GM126049 to J.X.C the National Science Foundation grant CBET-0939511 STC(to G.P.)
摘 要：Phase-contrast microscopy converts the phase shift of light passing through a transparent specimen,e.g.,a biological cell,into brightness variations in an *** ability to observe structures without destructive fixation or staining has been widely utilized for applications in materials and life *** these advantages,phase-contrast microscopy lacks the ability to reveal molecular *** address this gap,we developed a bond-selective transient phase(BSTP)imaging technique that excites molecular vibrations by infrared light,resulting in a transient change in phase shift that can be detected by a diffraction phase *** developing a time-gated pump-probe camera system,we demonstrate BSTP imaging of live cells at a 50 Hz frame rate with high spectral fidelity,sub-microsecond temporal resolution,and sub-micron spatial *** approach paves a new way for spectroscopic imaging investigation in biology and materials science.
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