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An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology

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Chinese Journal of Polymer Science 2023年第3期41卷 327-333,I0005页

作者： jun-Xiang Zhou Xue Yin hao-jun liang Department of Polymer Science and Engineering Hefei National Research Center for Physical Sciences at the Microscale iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) University of Science and Technology of China

At present,the coronavirus disease 2019(COVID-19)pandemic is a global health *** all over the globe are urgently looking forward to an effective solution to prevent the spread of the epidemic and avoid more casualties at an early *** this study,we establish an effective platform for the prevention of SARS-CoV-2 by combining the neutralization strategy and RNAi *** protect normal cells from infection,the customized cells are constructed to stably express viral antigenic receptor ACE2 on the cell *** modified cells are used as bait for inducing the viral *** transcription and replication activities of viral genome are intercepted subsequently by the intracellular sh RNAs,which are complementary to the viral gene fragments.A pseudotyped virus reconstructed from the HIV lentivirus is utilized as a virus model,by which we validate the feasibility and effectiveness of our strategy in *** work establishes an initial model and lays the foundation for future prevention and treatment of various RNA viruses.

关键词： SARS-CoV-2 Neutralization ACE2 RNAi Pseudotypedvirus

CAPABILITY OF DNA-FUELED MOLECULAR MACHINE IN TUNING ASSOCIATION RATE OF DNA-FUNCTIONALIZED GOLD NANOPARTICLES

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Chinese Journal of Polymer Science 2013年第9期31卷 1183-1183,1184-1189页

作者： Ting-jie Song hao-jun liang CAS Key Laboratory of Soft Matter Chemistry Hefei National Laboratory for Physical Sciences at the Microscale Department of Polymer Science and Engineering University of Science and Technology of China

The capability of our newly developed DNA-machine-driven strategy in tuning the association rate of DNA- AuNPs was compared with that of linker-addition strategy which has potential practical applications in different fields. The new established strategy shows its superiority to the linker-addition strategy in tuning the association rate of DNA-AuNPs in both pre-incubation treatment and non-incubation treatment conditions since its two components （a complex and a catalyst- oligonucleotide） can be individually optimized to make the machine run at an optimal rate. This strategy will provide a more convenient and flexible option in designing an oligonucleotide detection system and building a complex and versatile device.

关键词： Assembly DNA Enhancing Kinetics Nanoparticle.

Metal-Triggered DNA Folding by Different Mechanisms

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Chinese Journal of Chemical Physics 2015年第5期28卷 -页

作者： Wei Deng Bin Zheng Wei Ding Hong Zhu hao-jun liang Department of Polymer Science and Engineering University of Science and Technology of ChinaHefei 230026 China School of Chemistry and Chemical Engineering Hefei Normal University Hefei 230061 China Department of Polymer Science and Engineering University of Science and Technology of ChinaHefei 230026 ChinaCAS Key Laboratory of Soft Matter Chemistry University of Science and Technology of ChinaHefei 230026 China

Metal-mediated base pairs by the interaction between metal ions and artificial bases in oligonucleotides has been widely used in DNAnanotechnology and biosensing technique. Using isothermal titration calorimetry, circular dichroismspectroscopy and fluorescence spectroscopy, the folding process of T-C-rich oligonucleotides (TCO) induced by Hg2+ and Ag+ with the synthetic sequence d(T6C6T6C6T6C6T6) was studied and analyzed. Although thermodynamic data predict that TCO should initially fold into a relatively stable hairpin through two possible pathways of conformational transitions whether Hg2+ or Ag+ were added at first, the mechanisms and final products between the two are entirely different from isothermal titration calorimetry outcomes. When Hg2+ were added first, the haipin was formed through T-Hg-T structure with further stabilization by C-Ag-C after Ag+ addition. However, it is proposed that an unusual metal-base pair for Ag+ binding is generated instead classical C-Ag-C when Ag+ was injected first. Moreover, further confirmation of this unconventional metal-base pair T-Ag-C was verified by circular dichroism and fluorescence spectroscopy.

关键词： Metal-mediated base pairs Hg2+ and Ag+ T-C-rich oligonucleotides Folding mechanisms T-Ag-C base pair

Effect of Chain Configuration on Thermal Conductivity of Polyethylene-A Molecular Dynamic Simulation Study

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Chinese Journal of Polymer Science 2020年第12期38卷 1418-1425页

作者： Xiang Zhang Yu Wang Ru Xi Bin Wu Peng Chen Jia Sheng Qian hao-jun liang Anhui Province Key Laboratony of Environment friendly Polymer Materials SchoolofChemistry and Chemical EngineeringAnhui UniversitHefei 230601China Department of Polymer Science and Technology University of Science and Technology of ChinaHefei 230011China

Stretched polyethylene(PE)fibers are found to have super high thermal conductivity,while the bulk of polyethylene is usually thermal insulating even for those with high crystalline degree.A molecular dynamic simulation is deliberately carried out to examine the relationship between chain configuration and thermal conductivity of *** this simulation study,independent and interacting PE chains being stretched are compared with the aim to find out the effect of stretching on thermal conductivity of *** crystallization conditions for PE bulk are *** is found that heat transports predominately along the covalent chain rather than across chains in PE *** simulation study helps to understand experimental findings on thermal conductivity of PE at different states.W e also predict that amorphous PE may be super thermally conductive if chains are strictly stretched along heat flux.

关键词： Thermal conductivity Molecular dynamic simulation Polyethylene Chain configuration

Which is More Efficient as Compatibilizer： Di-block or Multiblock Copolymer？

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Chinese Journal of Chemical Physics 2006年第3期19卷 265-268页

作者： Da-chun Sun hao-jun liang Department of Educational Technology Baicheng Normal College Baicheng 137000 China Department of Polymer Science and Engineering University of Science and Technology of China Hefei 230026 China

Monte Carlo simulations were used to investigate the compatibilizing behaviors of multi-block copolymers with different architectures in A/B/（block copolymer） ternary blends. The volume fraction of homopolymer A, employed as the dispersed phase, was 19%. The simulations illustrate how a di- or multi-block copolymer aggregates at the interfaces and influences the phase behaviour of such incompatible polymer blends. The di-block copolymer chains tend to ＂stand＂ on the interface whereas the multi-block chains lie on the interface. In comparison with the di-block copolymer, the block copolymers with 4, or 10 blocks can occupy more areas on the interface, and thus the multi-block copolymers have higher efficiency for the retardation of the phase separation.

关键词： Monte Carlo simulation Compatibilizer Phase interface

Effects of individual shock wave therapy vs celecoxib on hip pain caused by femoral head necrosis

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World Journal of Clinical Cases 2023年第9期11卷 1974-1984页

作者： jun-Yu Zhu jun Yan Jian Xiao Hai-Guang Jia hao-jun liang Geng-Yan Xing Orthopedic Department The Third Medical Center of Chinese People’s Liberation Army General HospitalThe Armed Police Clinical CollegeAnhui Medical UniversityHefei 230022Anhui ProvinceChina Orthopedic Department The Third Medical Center of Chinese People’s Liberation Army General HospitalBeijing 100039China

BACKGROUND Celecoxib has been used to treat hip discomfort and functional difficulties associated with osteonecrosis of the femoral head(ONFH),although significant adverse reactions often follow long-term *** shock wave therapy(ESWT)can delay the progression of ONFH,alleviate the pain and functional limitations it causes,and avoid the adverse effects of *** To investigate the effects of individual ESWT,a treatment alternative to the use of celecoxib,in alleviating pain and dysfunction caused by *** This was a randomized,controlled,double-blinded,non-inferiority *** examined 80 patients for eligibility in this study;8 patients were excluded based on inclusion and exclusion criteria.A total of 72 subjects with ONFH were randomly assigned to group A(n=36;celecoxib+alendronate+sham-placebo shock wave)or group B(n=36;individual focused shock wave[ESWT based on magnetic resonance imaging three-dimensional(MRI-3D)reconstruction]+alendronate).The outcomes were assessed at baseline,at the end of treatment,and at an 8-wk *** primary outcome measure was treatment efficiency after 2 wk of intervention using the Harris hip score(HHS)(improvement of 10 points or more from the baseline was deemed sufficient).Secondary outcome measures were post-treatment HHS,visual analog scale(VAS),and Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)*** After treatment,the pain treatment efficiency of group B was greater than that of group A(69%vs 51%;95%CI:4.56%to 40.56%),with non-inferiority thresholds of-4.56%and-10%,***,the HHS,WOMAC,and VAS scores in group B dramatically improved during the follow-up period as compared to those in group A(P<0.001).After therapy,the VAS and WOMAC in group A were significantly improved from the 2nd to 8th wk(P<0.001),although HHS was only significantly altered at the 2 wk point(P<0.001).On the 1st d and 2nd wk after treatment,HHS and VAS scores were different

关键词： Extracorporeal shockwave therapy Osteonecrosis of femoral head Pain Magnetic resonance imaging three-dimensional reconstruction Celecoxib

Doxorubicin-loaded PLGA Microparticles with Internal Pores for Longacting Release in Pulmonary Tumor Inhalation Treatment

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Chinese Journal of Polymer Science 2015年第7期33卷 947-954页

作者： Tian-shi Feng 田华雨 Cai-na Xu Lin Lin Michael Hon-Wah Lam hao-jun liang Xue-si Chen CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of China Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of Sciences Department of Biology and Chemistry City University of Hong Kong Advanced Laboratory of Environmental Research and Technology(ALERT) Joint Advanced Research Center

Doxorubicin（DOX） loaded poly（lactic-co-glycolic acid）（PLGA） microparticles with internal pores（MP-D） were developed for long-acting release in pulmonary inhalation treatment. The PLGA microparticles exhibited favorable aerodynamic properties for pulmonary delivery. In vitro drug release profile suggested that MP-D have the advantage of long-term maintenance of drug concentrations. MTT assay demonstrated the in vitro anti-tumor efficiency of the DOX loaded PLGA microparticles. Furthermore, melanoma lung metastasis model was established to determine the in vivo antitumor efficiency. The mice treated with MP-D showed significantly fewer lesions than the untreated ones. The survival analysis indicated that MP-D prolonged the survival time of tumor-bearing mice. These results suggested that DOX loaded PLGA microparticles with internal pores have the potential to be used as long-acting release carriers in clinical lung cancer treatment.

关键词： Doxorubicin Poly(lactic-co-glycolic acid) Internal pores Long-acting release Pulmonary inhalation

哺乳动物细胞中mRNA起始密码子附近的发夹元件对基因表达的调控

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Chinese Journal of Chemical Physics 2023年第6期36卷 755-763,I0036-I0049,I0057页

作者：尹雪姚东宝梁好均中国科学技术大学高分子科学与工程系合肥230026 香港城市大学化学系中国香港999077

本文开发了一种位于起始密码子附近的发夹元件以在哺乳动物细胞中调节mRNA的表达水平.研究了发夹元件的热力学稳定性、GC含量、与5'帽的距离等参数对mRNA表达的影响.此外,隐藏在发夹元件内的起始密码子和上游开放阅读框也会削弱mRNA的... 详细信息

本文开发了一种位于起始密码子附近的发夹元件以在哺乳动物细胞中调节mRNA的表达水平.研究了发夹元件的热力学稳定性、GC含量、与5'帽的距离等参数对mRNA表达的影响.此外,隐藏在发夹元件内的起始密码子和上游开放阅读框也会削弱mRNA的翻译起始.证明了此简单的工程化发夹结构可以有效地用于可控的mRNA翻译调控,有利于在不同类型哺乳动物细胞中实现可预测的基因调控.

关键词：基因表达调控发夹元件起始密码子信使RNA

Investigation of transcriptional gene silencing and mechanism induced by shRNAs targeted to RUNX3 in vitro

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World Journal of Gastroenterology 2008年第19期14卷 3006-3014页

作者： Xue-Zhi Feng Xiu-Sheng He Ying-Zhi Zhuang Qiao Luo jun-hao .liang Shuai Yang Xue-Fang Tang Ju-Lin Liu Tao Chen Cancer Research Institute Nanhua University of South China Hengyang 421001 Hunan Province China Department of Oncology First Affiliated Hospital of Nanhua University of South China Hengyang 421001 Hunan Province China

AIM:To investigate transcriptional gene silencing induced by short hairpin RNAs (shRNAs) that target gene prompter regions of RUNX3 gene, and whether shRNAs homologous to DNA sequences may serve as initiators for methylation. METHODS:According to the principle of RNAi design, pSilencer3.1-H1-shRNA/RUNX3 expression vector was constructed, The recombinant plasmid shRNA was transfected into human stomach carcinoma cell line SGC7901 with Lipofectamine 2000. Then, the positive cell clones were screened by G418. The mRNA and protein expression level of RUNX3 in the stable transfected cell line SGC7901 were determined by RT-PCR, Western blotting and immunocytochemistry. Characteristics of the cell lines including SGC7901, pSilencer3.1-H1/SGC7901 and pSilencer3.1-H1-shRNA/RUNX3/SGC7901 were analyzed with growth curves, clone formation rate and cell-cycle distribution. The activated level of RUNX3 was examined after treatment with the different density of 5’-aza-2’-deoxycytidine (5-Aza-CdR) by using semi- quantitative RT-PCR and Western blotting. RESULTS:In the cell line SGC7901 transfected with pSilencer3.1-H1-shRNA/RUNX3, mRNA and proteinexpression of the RUNX3 gene was lost identified by RT- PCR, Western blotting and immunocytochemistry assay. The growth of pSilencer3.1-H1-shRNA/ RUNX3/SGC7901 cells without expression of RUNX3 was the fastest (P < 0.05), its rate of clone formation was the highest (P < 0.01), and the cell distribution in G0/G1 and S/M phases was lowest and highest, respectively (P < 0.05), compared with that of the transfected pSilencer3.1-H1 and non-transfected cells. Through RT-PCR and Western blot assay, inactivated RUNX3 could not be reactivated by 5-Aza-CdR. CONCLUSION:We found that, although shRNAs targeted to gene prompter regions of RUNX3 could effectively induce transcriptional repression with chromatic changes characteristic of inaction promoters, this was independent of DNA methylation, and the presence of RNA-dependent transcriptional silencin

关键词： RNA interference Short hairpin RNAs Promoter DNA methylation RUNX3 Stomach carcinoma