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Identification of Resistance to Pathogenesis Related Protein GmPR1L in Tobacco Botrytis cinerea Infection

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Phyton-International Journal of Experimental Botany 2023年第6期92卷 1907-1920页

作者： Yeyao Du Ye zhang Yang Song Zhuo zhang Sujie Fan hanzhu zhang Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchunChina Northeast Institute of Geography and Agroecology Chinese Academy of SciencesChangchunChina

Soybean(Glycine max(Linn.)Merr.)annual leguminous crop is cultivated all over the *** occurrence of diseases has a great impact on the yield and quality of *** this study,based on the RNA-seq of soybean variety M18,a complete CDS(Coding sequence)GmPR1L of the pathogenesis-related protein 1 family was obtained,which has the ability to resist fungal *** overexpression vector and interference expression vector were transferred into tobacco NC89,and the resistance of transgenic tobacco(Nicotiana tabacum L.)to Botrytis cinerea infection was *** results show that:Compared with the control,the activities of related defense enzymes SOD(Superoxide dismutase),POD(Peroxidase),PAL(L-phenylalanine ammonia-lyase)and PPO(Polyphenol oxidase)in the over-expressed transgenic tobacco OEA1 and OEA2 increased to different degrees,and increased significantly at different infection time *** activities of defense enzymes in the interfering strains IEA1 and IEA2 were significantly lower than those in the control *** results of resistance level identification showed that the disease spot rate of OEA1 was significantly lower than that of the control line,and the disease spot rate of OEA2 was significantly lower than that of the control *** plaque rate of the interfering expression line IEA1-IEA2 was significantly higher than that of the control *** is preliminarily believed that the process related protein GmPR1L can improve the resistance of tobacco to ***.

关键词： Soybean pathogenesis-related protein defense enzymes Botrytis cinerea

The Improvement of Soybean Salt Tolerance by Overexpressed GmPAO1

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1109-1124页

作者： Yeyao Du Yang Song Ye zhang Sujie Fan hanzhu zhang Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun130118China

Polyamines play an important regulatory role during plant growth and development and adversity stress,and polyamine oxidase(PAO)is involved in polyamine *** this study,an up-regulated polyamine oxidase gene GmPAO1 was obtained by transcriptome sequencing analysis and screening at soybean seedling ***,its expression pattern and function were *** identification results of transgenic GmPAO1 soybean positive lines showed that the relative expression level of GmPAO1 in the overexpressed lines was increased under salt *** increasing stress concentration,the seed germination rate ***,the seed germination rate of the overexpressed lines was significantly higher than that of the control lines,and the phenotypic character of the root systems was also better than that of the control *** measurement of superoxide dismutase(SOD)and peroxidase(POD)activities and malondialdehyde and hydrogen peroxide contents revealed that the overexpressed soybean lines significantly increased the SOD and POD activities,significantly reducing the malondialdehyde *** the hydrogen peroxide content in the transformed plants gradually increased,the hydrogen peroxide content in the overexpression lines was still lower than that in the gene editing *** on this,it was preliminarily judged that GmPAO1 can improve soybean salt tolerance.

关键词： Soybean polyamine oxidase salt tolerance overexpressed

Microstructure and strengthening mechanism of hot-extruded ultralight Mg-Li-Al-Sn alloys with high strength

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Journal of Materials Science & Technology 2022年第8期103卷 186-196页

作者： Gang Zhou Yan Yang hanzhu zhang Faping Hu Xueping zhang Chen Wen Weidong Xie Bin Jiang Xiaodong Peng Fusheng Pan International Joint Laboratory for Light Alloys(MOE) College of Materials Science and EngineeringChongqing UniversityChongqing 400044China National Engineering Research Center for Magnesium Alloys Chongqing UniversityChongqing 400044China State Key Laboratory of Mechanical Transmissions College of Materials Science and EngineeringChongqing UniversityChongqing 400044China Beijing Spacecrafts Beijing 100090China

Duplex-structured Mg-7Li-2Al-1.5Sn alloys with high strength were fabricated and their strengthening mechanism was *** Mg-7Li-2Al-1.5Sn alloys were prepared by casting and extruded at the temperature of 533 K with an extrusion ratio of 25:*** microstructure and mechanical properties of Mg-7Li-2Al-1.5Sn alloys were systematically investigated by OM,XRD,SEM,TEM,and tensile *** results show that Mg-7Li-2Al-1.5Sn alloys are mainly composed ofα-Mg,β-Li,LiMgAl_(2),Mg_(2)Sn and Li_(2)MgSn *** yield strength(YS),ultimate tensile strength(UTS)and elongation(EL)of the extruded alloy at room temperature reach 250 MPa,324 MPa and 11.9%,respectively.A lot of Sn-rich precipitates(Mg_(2)Sn and Li_(2)MgSn)are precipitated during extrusion with an average size of∼14 nm,which is beneficial to the grain *** recrystallization occurs during hot deformation and the nanoprecipitates effectively refine the dynamic recrystallized(DRXed)***,the residual dislocations existed in DRXed and un-DRXed grains result in the dislocation strengthening in the extruded ***7Li-2Al-1.5Sn alloys possess excellent high-temperature mechanical properties with the YS,UTS and EL of 200 MPa,237 MPa and 26.7%at 423 K,***-rich precipitates with good thermal stability can effectively prevent grain growth,which is good for the improvement of the high-temperature performance of Mg-Li-Al-Sn alloy.

关键词： Ultralight Mg-Li alloy Microstructure Mechanical properties Precipitation Strengthening mechanism

The response of roots and the rhizosphere environment to integrative cultivation practices in paddy rice

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Journal of Integrative Agriculture 2024年第6期23卷 1879-1896页

作者： hanzhu Gu Xian Wang Minhao zhang Wenjiang Jing Hao Wu Zhilin Xiao Weiyang zhang Junfei Gu Lijun Liu Zhiqin Wang Jianhua zhang Jianchang Yang Hao zhang Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College Yangzhou UniversityYangzhou 225009China Taizhou Institute of Agricultural Science Jiangsu Academy of Agricultural SciencesTaizhou 225300China State Key Laboratory of Agrobiotechnology School of Life SciencesThe Chinese University of Hong KongShatinHong Kong 999077China Department of Biology Faculty of ScienceHong Kong Baptist UniversityKowloonHong Kong 999077China

Integrative cultivation practices(ICPs)are essential for enhancing cereal yield and resource use ***,the effects of ICP on the rhizosphere environment and roots of paddy rice are still poorly *** this study,four rice varieties were produced in the *** variety was treated with six different cultivation techniques,including zero nitrogen application(0 N),local farmers’practice(LFP),nitrogen reduction(NR),and three progressive ICP techniques comprised of enhanced fertilizer N practice and increased plant density(ICP1),a treatment similar to ICP1 but with alternate wetting and moderate drying instead of continuous flooding(ICP2),and the same practices as ICP2 with the application of organic fertilizer(ICP3).The ICPs had greater grain production and nitrogen use efficiency than the other three *** length,dry weight,root diameter,activity of root oxidation,root bleeding rate,zeatin and zeatin riboside compositions,and total organic acids in root exudates were elevated with the introduction of the successive cultivation *** enhanced nitrate nitrogen,the activities of urease and invertase,and the diversity of microbes(bacteria)in rhizosphere and non-rhizosphere soil,while reducing the ammonium nitrogen *** nutrient contents(ammonium nitrogen,total nitrogen,total potassium,total phosphorus,nitrate,and available phosphorus)and urease activity in rhizosphere soil were reduced in all treatments in comparison with the non-rhizosphere soil,but the invertase activity and bacterial diversity were *** main root morphology and physiology,and the ammonium nitrogen contents in rhizosphere soil at the primary stages were closely correlated with grain yield and internal nitrogen use *** findings suggest that the coordinated enhancement of the root system and the environment of the rhizosphere under integrative cultivation approaches may lead to higher rice production.

关键词： rice(Oryza sativa L.) nitrogen use efficiency grain yield integrative cultivation practices roots

两种转基因方法对转GmXTH抗旱基因大豆根系的影响

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分子植物育种 2023年第13期21卷 4278-4286页

作者：张瀚竹张野杜叶垚宋阳吉林农业大学农学院长春130118

为分析干旱胁迫下,两种不同方法得到的转基因大豆抗旱性差异,本试验选用农杆菌介导法得到的转GmXTH基因大豆品系OEA1、OEA2、OEA3、OEA4和花粉管通道转化得到的转基因大豆品系OET1、OET2、OET3、OET4和对照M18为试验材料,设置不同浓度... 详细信息

为分析干旱胁迫下,两种不同方法得到的转基因大豆抗旱性差异,本试验选用农杆菌介导法得到的转GmXTH基因大豆品系OEA1、OEA2、OEA3、OEA4和花粉管通道转化得到的转基因大豆品系OET1、OET2、OET3、OET4和对照M18为试验材料,设置不同浓度梯度的PEG-6000模拟干旱胁迫的实验环境,鉴定苗期抗旱能力的差异。结果表明,随着干旱胁迫加重,两种不同转基因方法得到的转基因株系间叶片含水量、POD活性、SOD活性、丙二醛含量之间无显著差异,转基因大豆品系的抗旱能力比对照M18有显著提高,但不同转基因方法得到的大豆品系之间的抗旱能力无差异。

关键词：大豆根系农杆菌介导法花粉管通道抗旱

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船用脱硫塔舾装管件安装建模研究

江苏科技大学学报（自然科学版） 2023年第6期37卷 6-13页

作者：张汉柱王岳蒋志勇江苏科技大学船舶与海洋工程学院镇江212100

为提高船用脱硫塔改装效率,对船用脱硫塔改装过程中的舾装管件进行研究,通过仿真建模验证管件安装计划的合理性,给出了船用脱硫塔舾装过程优化方法,保障舾装管件的安装效率与质量.

关键词：脱硫塔改装管件安装计划仿真改进方法

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校验用开合式霍尔电流传感器聚磁环设计

电测与仪表 2022年第2期59卷 171-175页

作者：张涵竹李岩松刘君华北电力大学(北京)电气与电子工程学院北京102206

对电流互感器进行在线校验符合电力系统供电可靠性的要求,针对一般闭环霍尔电流传感器磁环只开一段气隙的情况,文中介绍了一种可用于在线校验的开合式聚磁环结构霍尔电流传感器。以提升测量精度和灵敏度为目的,从磁环材料的选择、磁环... 详细信息

对电流互感器进行在线校验符合电力系统供电可靠性的要求,针对一般闭环霍尔电流传感器磁环只开一段气隙的情况,文中介绍了一种可用于在线校验的开合式聚磁环结构霍尔电流传感器。以提升测量精度和灵敏度为目的,从磁环材料的选择、磁环横截面积大小以及气隙长度等方面阐述了设计方法,具体探讨了两段气隙总宽度与磁环总长度的比例关系。利用COMSOL Multiphysics对开合式聚磁环模型进行仿真。实验结果表明,此种聚磁环结构能使霍尔电流传感器具有更广的线性测量范围,且灵敏度比单气隙结构更高。实验结论为今后进行现场在线校验提供了理论参考。

关键词：霍尔传感器聚磁环开合式在线校验

炭黑种类和用量对磁性丁腈橡胶胶料性能的影响

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橡胶工业 2023年第2期70卷 111-117页

作者：梁琳张寒珠张保岗丁乃秀刘光烨青岛科技大学高性能聚合物及成型技术教育部工程研究中心山东青岛266042

研究炭黑种类和用量对磁性丁腈橡胶(NBR)胶料性能的影响。结果表明:分别将30份炭黑220,N330,N550,N660,N774和N990填充于磁性NBR胶料中,填充炭黑N660的磁性NBR混炼胶的Payne效应最弱(填料网络结构最稳定),填充炭黑N220的磁性NBR硫化胶... 详细信息

研究炭黑种类和用量对磁性丁腈橡胶(NBR)胶料性能的影响。结果表明:分别将30份炭黑220,N330,N550,N660,N774和N990填充于磁性NBR胶料中,填充炭黑N660的磁性NBR混炼胶的Payne效应最弱(填料网络结构最稳定),填充炭黑N220的磁性NBR硫化胶的物理性能最佳,填充炭黑N550的磁性NBR硫化胶的磁性能最优[剩磁(B)r为0.220 T,矫顽力(Hcb)为165 kA·m-1,最大磁能积(BH)max为9.3 kJ·m^(-3)];分别将不同用量炭黑N550填充于磁性NBR胶料中,填充20份炭黑N550的磁性NBR混炼胶的尺寸稳定性最好,填充30份炭黑N550的磁性NBR硫化胶的拉伸强度最大(9.1 MPa),填充10份炭黑N550的磁性NBR硫化胶的磁性能最优[Br为0.228 T,Hcb为171 kA·m-1,(BH)max为9.9 kJ·m^(-3)]。

关键词：炭黑丁腈橡胶磁性橡胶物理性能磁性能

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磁粉并用对磁性丁腈橡胶胶料性能的影响

橡胶工业 2023年第4期70卷 260-265页

作者：梁琳张寒珠张保岗丁乃秀刘光烨青岛科技大学高性能聚合物及成型技术教育部工程研究中心山东青岛266042

以铁氧体磁粉和钕铁硼(NdFeB)磁粉为磁性填料制备磁性丁腈橡胶(NBR)胶料,研究两种磁粉单用和并用对胶料性能的影响。结果表明,两种磁粉单用时,采用NdFeB磁粉制备的磁性NBR胶料的磁性能更优异。两种磁粉并用时,随着NdFeB磁粉用量的增大,... 详细信息

以铁氧体磁粉和钕铁硼(NdFeB)磁粉为磁性填料制备磁性丁腈橡胶(NBR)胶料,研究两种磁粉单用和并用对胶料性能的影响。结果表明,两种磁粉单用时,采用NdFeB磁粉制备的磁性NBR胶料的磁性能更优异。两种磁粉并用时,随着NdFeB磁粉用量的增大,胶料的硬度增大,拉伸强度和撕裂强度先增大后减小,在铁氧体磁粉/NdFeB磁粉并用比为700/10时达到最大;胶料的剩余磁感应强度和最大磁能积先增大后减小,在铁氧体磁粉/NdFeB磁粉并用比为700/30时最大,分别为0.197 T和7.3 kJ·m^(-3)。

关键词：铁氧体磁粉钕铁硼磁粉并用磁性丁腈橡胶磁性能

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