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Salinity Stress in Roots of Contrasting BarleyGenotypes Reveals Time-Distinct and Genotype-Specific Patterns for Defined Proteins

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Molecular Plant 2014年第2期7卷 336-355页

作者： Katja Witzel Andrea Matros Marc Strickert Stephanie Kaspar Manuela Peukert Karl H. Mühling Andreas Börner hans-peter mock Leibniz Institute of Plant Genetics and Crop Plant Research Corrensstrasse 3 06466 Gatersleben Germany Present address: Leibniz Institute of Vegetable and Ornamental Crops Theodor-Echtermeyer-Weg 1 14979 Großbeeren Germany Present address: University of Siegen Media Systems Group Hölderlinstrasse 3 57068 Siegen Germany Present address: Bruker Daltonik GmbH Fahrenheitstrasse 4 28359 Bremen Germany Institute of Plant Nutrition and Soil Science Christian Albrechts University Hermann-Rodewald-Strasse 2 24118 Kiel Germany

Soil salinity is one of the most severe abiotic stress factors threatening agriculture worldwide. Hence,particular interest exists in unraveling mechanisms leading to salt tolerance and improved crop plant performance onsaline soils. Barley is considered to be one of the most salinity-tolerant crops, but varying levels of tolerance are wellcharacterized. A proteomic analysis of the roots of two contrasting cultivars （cv. Steptoe and cv. Morex） is *** plants were exposed to a period of 1, 4, 7, or 10 d at 0, 100, or 150mM NaCI. The root proteome was analyzedbased on two-dimensional gel electrophoresis. A number of cultivar-specific and salinity stress-responsive proteins wereidentified. Mass spectrometry-based identification was successful for 74 proteins, and a hierarchical clustering analysisgrouped these into five clusters based on similarity of expression profile. The rank product method was applied to sta-tistically access the early and late responses, and this delivered a number of new candidate proteins underlying salinitytolerance in barley. Among these were some germin-like proteins, some pathogenesis-related proteins, and numerousas-yet uncharacterized proteins. Notably, proteins involved in detoxification pathways and terpenoid biosynthesis weredetected as early responsive to salinity and may function as a means of modulating growth-regulating mechanisms andmembrane stability via fine tuning of phytohormone and secondary metabolism in the root.

关键词： barley genotypes mass spectrometry proteome analysis salinity stress two-dimensional gel electrophoresis

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A computation design method for architectural artifacts adapted from traditional Kagome bamboo basketry techniques

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Frontiers of Architectural Research 2024年第2期13卷 249-264页

作者： Hiroyuki Shinohara Tung Hoi peter Chan School of Architecture The Chinese University of Hong KongHong KongChina

This study is a digital form-finding and manual fabrication experiment in woven architectural design,with one traditional weaving style,Kagome,used to scale the craft up into an architectural-scale bamboo woven *** is a trihexagonal pattern employed in traditional bamboo basketry as a triaxial weaving system,resulting in an object with a self-bracing capacity without the use of fasteners owing to its interlacing *** existing studies and tools have addressed triaxial weaving design and representation,the current consideration of the advantages of weaving with bamboo is *** address this research gap,this study develops a computational design method based on studies on bamboo *** allows for the representation and exploration of design geometries using combinations of regular triangular meshes for the fabrication of Kagome woven bamboo artifacts.A full-scale mock-up was fabricated to evaluate the effectiveness of the *** mock-up demonstrated the self-bracing properties of Kagome,but there were discrepancies between the mock-up and the *** affecting bamboo weaving on an architectural scale have been identified within this study to inform future research on woven bamboo structures.

关键词： Traditionalbamboo craft Triaxialmaterial interlacing Bamboo Computational design methods Mesh block combinationsystem Architecturalwoven artifact

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Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains

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Virologica Sinica 2014年第3期29卷 176-182页

作者： Dipongkor Saha Uladzimir U.Karniychuk Liping Huang Marc Geldhof Merijn Vanhee David J.Lefebvre peter Meerts Richard Ducatelle Jan V.Doorsselaere hans J.Nauwynck Laboratory of Virology Department of Virology Parasitology and Immunology Faculty of Veterinary Medicine Ghent UniversityBelgium Brain Tumor Research Center Department of Molecular Neurosurgery Harvard Medical School and Massachusetts General Hospital 185 Cambridge StreetBoston MA-02114 USA Division of Experimental Therapeutics Toronto General Hospital Research Institute University Health NetworkToronto Canada Division of Swine Infectious Diseases Harbin Veterinary Research Institute The Chinese Academy of Agricultural Sciences Department of Health Care and Biotechnology KATHO Catholic University College of South-West FlandersBelgium CODA-CERVA Groeselenberg 99 1180 Ukkel Belgium Ablynx nv Technologiepark 219052 Zwijnaarde Belgium Department of Pathology Bacteriology and Poultry Diseases Faculty of Veterinary Medicine Ghent UniversityBelgium

VC2002, isolated from postweaning multisystemic wasting syndrome(PMWS)-affected pig, is a mixture of two porcine circovirus genotype 2b(PCV2b) viruses, K2 and K39. Preliminary experiments disclosed short-term adverse effects of K39, but not K2, on porcine foetuses. These findings led to the hypothesis that infection of immuno-incompetent foetuses with K2 confers a status of immunotolerance, and postnatal super-infection with K39 triggers PMWS. To explore this hypothesis, nine 55-day-old foetuses were inoculated in utero(three with K2-104.3TCID50, three with K39-104.3TCID50 and three with medium), and foeto-pathogenicity examined. At 21 days post-inoculation(dpi), K2 did not induce pathology, whereas pathological effects of K39 were evident. Twenty-four 45-day-old foetuses were subsequently inoculated to examine the long-term effect of K2, including six with K2-high dose-104.3TCID50, six with K2-low dose-102.3TCID50 and 12 mock-inoculated controls. Both doses resulted in five mummified foetuses and one live-born piglet each(69dpi). K2 was recovered from all mummies. K2 and K2-specific antibodies were not detected in serum of the two live-born piglets at birth, indicating full control of K2 infection. The K2-low dose-infected piglet was immunostimulated at day 2, but not the K2-high dose-infected piglet. Both non-stimulated and stimulated K2-infected piglets were super-inoculated with K39 at day 6 or 8(taken as 0 days post super-inoculation). Low viral replication was observed in the non-stimulated K2-K39 piglet(up to 103.3 TCID50/g; identified as K39). In contrast, viral replication was extremely high in the stimulated K2-K39 piglet(up to 105.6TCID50/g) and identified as K2, indicating that K2 infection is controlled during foetal life, but emerges after birth upon immunostimulation. However, none of the piglets showed any signs of PMWS.

关键词： PCV2 immunotolerance PMWS porcine foetuses

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Hybrid simulation using implicit solver coupling with HLA and FMI

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International Journal of Modeling, Simulation, and Scientific Computing 2017年第4期8卷 224-244页

作者： Muhammad Usman Awais Milos Cvetkovic and peter Palensky National University of Computing and Emerging Science LahorePakistan Department of Electrical Engineering Mathematics and Computer Science TU Delft Building 36Room B36-LB 03.230 Mekelweg 42628CD DelftNetherlands

Hybrid systems such as Cyber Physical System(CPS)are becoming increasingly popular,mainly due to the involvement of information technology in different aspects of *** analysis and verification of hybrid system models,simulation is used *** parts of a common hybrid system may belong to different domains of study,it is sometimes beneficial to use specialized simulation packages(SPs)for each *** this case,parts of a system are simulated in different *** idea may seem simple,but coupling more than one simulation component presents challenges related to numerical *** presented article suggests an implicit solver coupling technique enhanced to facilitate simulation of hybrid models using multiple simulation *** technique is developed using two of the most popular simulation interoperability standards,namely,the High Level Architecture and the Functional mock-up *** using these standards,the developed algorithm will be useful for a large number of practitioners and *** developed algorithm is described using a generic distributed computation model,which makes it reproducible even without using the *** the verification of results,the algorithm is tested on two case *** results are compared to a monolithic simulator and the proximity of results initiates the validity of the developed algorithm.

关键词： Distributed simulation algorithms co-simulation distributed simulation parallel simulation continuous simulation simulation interoperability DEVS OpenModelica Modelica

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Textual Anticipation and the Putative Reader in Persuasive Discourse

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外国语 2021年第1期44卷 2-20页

作者： peter R.R.White University of New South Wales SydneyAustralia

The notion that’monologic’written texts construe for themselves a putative addressee(variously termed the’ideal’,’imagined’,’virtual’,’intended’,’model’or’mock’reader)has received a great deal of scholarly attention,initially by scholars of literary fiction and subsequently by discourse analysts more *** paper is concerned with putative-reader positioning in persuasive texts and with how,through observing the relationships of alignment and dis-alignment which the author enters into with this putative reader,it is possible to better understand the rhetorical workings of these *** specifically,it demonstrates how,through an analysis of which beliefs,expectations and attitudes the author projects onto this putative addressee it is possible to better understand how such a text may be persuasive,to more systematically describe the’compliant readings’associated with a text and to more thoroughly deal with’ideological’workings of persuasive texts as they naturalize particular value systems and world *** outlining and demonstrating an approach to analyses of putative-addressee positioning,the paper draws on prior work in literary criticism scholarship and in university composition *** primary focus,however,is on developing prior work on the putative addressee in the appraisal-framework literature,in particular work which has attended to the role of the resources of dialogistic positioning(ENGAGEMENT)in’writing the reader into the text’.

关键词： persona persuasion dialogism readership

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Transporting Mammalian Cells at Ambient Temperature:A Viable Alternative to Dry Ice

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Advances in Bioscience and Biotechnology 2017年第4期8卷 127-133页

作者： Steingrimur Stefansson Seunghyun Han Ye In Jeon David S.Chung peter Hwang Huyen Le John L.Warden David Ho HeMemics Biotechnologies Inc RockvilleUSA Johns Hopkins University BaltimoreUSA George Washington University WashingtonDCUSA Division of Biology and Medicine Brown UniversityProvidenceUSA Thomas Jefferson High School for Science and Technology AlexandriaUSA Nauah Solutions McleanUSA

The most common method of shipping cells between institutes and companies is sending them frozen, usually treated with anti-freeze solution (most commonly DMSO because it is less toxic than many alternatives), and then packaging them in dry ice for shipment. However many countries place restrictions on dry ice shipments. An alternative to shipping frozen cell vials is to send flasks of growing cells in media. This also has problems because cells in media have limited viability and the flasks can leak. Here we report on an alternative method for shipping viable cells at ambient temperature without dry ice or in media filled flasks. In this study we report on the development and properties of HemSol?. This is an inexpensive, eco-friendly and protects cell integrity at ambient temperature while maintaining viability. We have previously shown that HemSol? protects platelet and RBC function in cold storage and circulating tumor cells up to 6 days. Therefore we wanted to know if HemSol? could also be used to transport live cells. Since HemSol? is a liquid, we experimented with encasing the cells with HemSol? and gelatin so as to prevent dry ice shipment of cells and circumvent the shipping of cells in media. We performed mock shipping experiments where cells were stored in HemSol? gel kept at room temperature on a lab benchtop and cells stored in dry ice was also kept on lab benchtop for up to 2 days. After the mock shipping period, we analyzed cells for their functions. Our results show that cells in HemSol? gel have greater than 95% viability and restored biological functions in 2 hours, whereas, cells shipped in dry ice required more than 24 hours to recover and needed media change to remove the DMSO.

关键词： HemSol^(TM) HemSol^(TM) Gel Transportation Short Term Storage Cells Shipment Dry Ice Ambient Temperature Shipment

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调对TMD慢性疼痛治疗效果的临床评价初探

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临床口腔医学杂志 2007年第1期23卷 45-47页

作者：黎腾聪王美青 Kelun Wang peter Svensson 第四军医大学口腔医学院解剖生理教研室陕西西安710032 Oral facial Pain Laboratory Center for Sensory-Motor Interaction Aalborg UniversityDenmark Department of Clinical Oral Physiology Royal Dental College University of AarhusDenmark

目的:探讨调治疗颞下颌关节紊乱病(TMD)慢性疼痛的疗效。方法:TMD伴慢性口颌面痛患者21例,盲法随机分为治疗组(10例)和对照组(11例),治疗组予以本课题组所报道的方法进行调治疗,以建立平稳咬合接触为目标,对照组作模拟调。检测治... 详细信息

目的:探讨调治疗颞下颌关节紊乱病(TMD)慢性疼痛的疗效。方法:TMD伴慢性口颌面痛患者21例,盲法随机分为治疗组(10例)和对照组(11例),治疗组予以本课题组所报道的方法进行调治疗,以建立平稳咬合接触为目标,对照组作模拟调。检测治疗前、治疗后即刻和治疗1月时疼痛指数(VAS)、开口度及咬合力水平。结果:治疗组VAS降低(p=0.000),开口度增加(p=0.008),咬合力有所增加(p=0.050),与对照组相差显著。对照组治疗前后无明显差异。结论:以建立自然牙列稳定咬合接触关系为目的的调治疗可以明显缓解TMD慢性疼痛。

关键词：调[牙合] 颞下颌关节紊乱病慢性疼痛

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