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Overproduction of Alkaloid Phytoalexins in California Poppy Cells Is Associated with the Co-Expression of Biosynthetic and Stress-Protective Enzymes

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Molecular Plant 2010年第5期3卷 927-939页

作者： Sornitza Angelova Marcus Buchheim Doreen Frowitter Angelika Schierhorn Werner roos Kliment-Ohridski-University of Sofia Faculty of Biology Institute of Molecular Biology Sofia Bulgaria Martin-Luther-University of Halle-Wittenberg institute of Pharmacy Department of Pharmaceutical Biology and Pharmacology Laboratory of Molecular Cell Biology Kurt Mothes Str. 3 06120 Halle (Saale) Germany Max-Planck-Research group 'Enzymatics of Protein Folding' Laboratory of Mass Spectrometry Halle (Saale) Germany

Many plant cells respond to pathogens by the induction of phytoalexin biosynthesis, but the underlying changes of gene expression are often obscured by their close linkage to the complex rearrangements during pathogen defense, especially the hypersensitive cell death. In root-derived cell cultures of Eschscholzia californica, the overproduction of cytotoxic benzophenanthridine alkaloids can be triggered by a minimum of pathogen pressure that does not evoke hypersensitive reactions. Such conditions activate a signal chain that is initiated by a short contact to low concentrations of yeast glycoprotein elicitor and includes a transient acidification of the cytoplasm. In contrast, high elicitor concentrations signal via an increase of jasmonate and trigger hypersensitive cell death, preceded by a drastic decay of translatable mRNAs. The main changes in protein and mRNA patterns caused by either signal path were compared by 2D proteomic separation, MS/MS sequencing and mRNA-in vitro translation. The four proteins showing the highest overexpression were identical between cells that received low or high-elicitor treatment and overlapped with the three proteins most upregulated by artificial pH shifts. They comprised one biosynthetic enzyme （norcoclaurine：SAM 4＇ O-methyl-transferase） plus a unique combination of stress-protective proteins： a heat shock protein （hsp 70）; a peptidyl-prolyl-cis/trans isomerase （cyclophilin）; and a glyceraldehyde-3-phosphate dehydrogenase. It appears that overproduction of the benzophenanthri- dine phytoalexins requires the up-regulation of a rate-limiting biosynthetic enzyme plus the coordinated expression of a specific set of protective enzymes and thus is managed like an oxidative stress.

关键词： Benzophenanthridine alkaloid biosynthesis signal transduction stress protection functional proteomics pH signaling.

genetic Testing in Professional Football:Perspectives of Key Stakeholders

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Journal of Science in Sport and Exercise 2022年第1期4卷 49-59页

作者： Alexander B.T.McAuley David C.Hughes Loukia g.Tsaprouni Ian Varley Bruce Suraci homas R.roos Adam J.Herbert Adam L.Kelly Faculty of Health Education and Life SciencesBirmingham City UniversityBirminghamWest MidlandsUK Department of Sport Science Nottingham Trent UniversityNottinghamUK Academy Coaching Department AFC BournemouthBournemouthUK The International Academy of Sports Science and Technology(AISTS) University of LausanneLausanneSwitzerland Department of Life Sciences Birmingham City UniversityCity South CampusWestbourne RoadEdgbastonBirmingham B153TNUK

Purpose genetic research in football is currently in it’s infancy but is growing ***,the practical application of genetic testing in football and the views concerning its use are ***,the purpose of this study was to assess the current practical application of genetic testing in professional football and provide an insight into the perspectives of key stakeholders(i.e.,coaches,practitioners,players).Methods In total,122 participants completed an online anonymous *** consisted of 21 multiple choice and Likert scale questions,with the option of providing an explanation for each *** Findings revealed genetic testing is rarely utilised by key stakeholders(10%)or their respective organisations(14%).However,three quarters(75%)had the opinion that genetic testing will have great utility in the *** majority(72%)believed genetic testing should be used for athlete development and injury risk,whilst 35%believed that genetic testing should be utilised for talent identification ***,most key stakeholders viewed their own(89%)and their col-leagues’(79%)knowledge related to genetic testing as insufficient;mainly due to ineffective current communication methods(91%).Most believed educational workshops are required(71%),whilst nearly all(91%)were interested in developing their expertise on the utility of genetic *** genetic testing is rarely used within professional football,although key stakeholders anticipate that it will be utilised more in the *** such,educational support may prove valuable in improving key stakeholder knowledge and the practical application of genetic testing in professional football.

关键词： Soccer Talent identification Athlete development genetics

Cartan-Hartogs域的Khler-Einstein度量

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中国科学（A辑） 2006年第11期36卷 1201-1233页

作者：王安殷慰萍张利友 roos guy 首都师范大学数学科学学院 Nevski prospekt 113/4-53 191024 St PetersburgRussian Federation

研究以不可约有界对称域Ω为底空间的一类Hartogs域(?)上的K(?)hler- Einstein度量,这种域称之为Cartan-Hartogs域,是华罗庚域的一种,其中K(?)hler- Einstein度量的生成函数满足一带有边界条件的复Monge-Ampère方程．一般地,域(?)是非... 详细信息

研究以不可约有界对称域Ω为底空间的一类Hartogs域(?)上的K(?)hler- Einstein度量,这种域称之为Cartan-Hartogs域,是华罗庚域的一种,其中K(?)hler- Einstein度量的生成函数满足一带有边界条件的复Monge-Ampère方程．一般地,域(?)是非齐性域,其上有一全纯自同构子群以及群不变轨道X∈[0,1[,因此可以把复Monge-Ampère方程化为常微分方程,并且此方程在临界值μ_0=μ时能够显式解出．临界值μ_0对于研究其他不变度量如Bergman度量也是非常有意义的．文中还给出一个猜想,并且证明了该猜想对于两类两类例外域是成立的．

关键词： Hartogs域 Kfihler—Einstein度量有界对称域例外域 Cartan—Hartogs域

A Systematic Review of the genetic Predisposition to Injury in Football

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Journal of Science in Sport and Exercise 2023年第2期5卷 97-115页

作者： Alexander B.T.McAuley David C.Hughes Loukia g.Tsaprouni Ian Varley Bruce Suraci Thomas R.roos Adam J.Herbert Daniel T.Jackson Adam L.Kelly Faculty of Health Education and Life SciencesBirmingham City UniversityBirminghamWest MidlandsUK Department of Sport Science Nottingham Trent UniversityNottinghamUK Academy Coaching Department AFC BournemouthBournemouthUK The International Academy of Sports Science and Technology(AISTS) University of LausanneLausanneSwitzerland Department of Life Sciences Birmingham City UniversityCity South CampusWestbourne RoadEdgbaston B153TNUK

Purpose Synthesise genetic association studies investigating injury involving football players to identify which genetic variants have the most empirical evidence to *** A comprehensive search of the PubMed,SPORTDiscus,and MEDLINE databases until March 11th 2022 identi-fied 34 *** criteria:primary investigations,included football players,examined the association of a genetic variant with injury,and were published in *** of bias was assessed using the Newcastle–Ottawa Scale.A narrative synthesis summarised *** There were 33 candidate gene studies and one genome-wide study,with 9642 participants across all studies(range=43–1311;median=227).Ninety-nine polymorphisms were assessed within 63 ***-one polymorphisms were associated with injury *** polymorphisms had their specific allelic associations with injury replicated twice in independent cohorts:ACTN3(rs1815739)XX genotype was associated with an increased susceptibility to non-contact muscle injuries,ACAN(rs1516797)g allele was associated with increased susceptibility to anterior cruciate ligament(ACL)injuries,and VEgFA(rs2010963)CC genotype was associated with an increased susceptibility to ACL and ligament or tendon ***,several methodological issues(e.g.,small sample sizes,cohort heterogeneity,and population stratification)are prevalent that limit the reliability and external validity of *** At present,the evidence base supporting the integration of genetic information as a prognostic or diagnosis tool for injury risk in football is *** participation of organisations in international consortia is suggested to combat the current methodological issues and subsequently improve clarity concerning the underlying genetic contribution to injury susceptibility.

关键词： Soccer genomics Polymorphism Snp Susceptibility

跨国产业供应链合作关键成功因素的实证研究——以中、德印刷产业为例

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管理工程学报 2013年第2期27卷 209-219页

作者：刁鸿珍李随成 Alexander roos 西安理工大学经济与管理学院陕西西安710048 斯图加特媒介大学德国斯图加特70569

通过国内外文献分析和深度访谈提取出了中、德印刷产业供应链合作的关键成功因素,在此基础上,分别从德方、中方和中德双方视角出发,运用探索性因素分析方法对关键成功因素进行实证研究,根据中、德双方认知的不同,关键成功因素分为一致... 详细信息

通过国内外文献分析和深度访谈提取出了中、德印刷产业供应链合作的关键成功因素,在此基础上,分别从德方、中方和中德双方视角出发,运用探索性因素分析方法对关键成功因素进行实证研究,根据中、德双方认知的不同,关键成功因素分为一致性因素和差异性因素,一致性因素是双方合作成功的基础,而差异性因素则是双方在合作中需要进一步适应和磨合的重点。

关键词：供应链合作跨国合作印刷产业关键成功因素

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Fe/MnPd薄膜各向异性和矫顽力的研究

物理学报 2000年第5期49卷 997-1001页

作者：唐云俊 B.F.P.roos B.Hillebrands 赵宏武詹文山中国科学院物理研究所与凝聚态物理中心磁学国家重点实验室北京100080 Physics Department University Kaiserslautern

从理论和实验上对Fe/MnPd薄膜的交换偏置效应、各向异性和矫顽力的增加作了系统的研究 .研究表明 ,在FM /AF结构中 ,FM和AF的交换耦合作用会诱导系统产生高阶各向异性 ,在Fe/MnPd结构中获得的单轴和立方各向异性的大小同单轴各向异性与... 详细信息

从理论和实验上对Fe/MnPd薄膜的交换偏置效应、各向异性和矫顽力的增加作了系统的研究 .研究表明 ,在FM /AF结构中 ,FM和AF的交换耦合作用会诱导系统产生高阶各向异性 ,在Fe/MnPd结构中获得的单轴和立方各向异性的大小同单轴各向异性与铁磁层的厚度成反比关系一样 .同时 ,铁磁层 (FM )和反铁磁层 (AF)

关键词： Fe/MnPd薄膜各向异性矫顽力

中德印刷产业对接合作关键成功因素的案例研究

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西安理工大学学报 2013年第1期29卷 114-120页

作者：刁鸿珍李随成 roos Alexander 西安理工大学经济与管理学院陕西西安710054 斯图加特媒介大学德国斯图加特70569

在对跨国产业合作的动因、关键成功因素以及供应链合作结构的文献回顾基础上,针对中、德印刷产业对接合作,选择MBO、曼罗兰、莱默尔E+L、界龙集团、大连盛道等企业作为对象进行跨案例研究。研究明确了中德印刷产业供应链合作的框架与合... 详细信息

在对跨国产业合作的动因、关键成功因素以及供应链合作结构的文献回顾基础上,针对中、德印刷产业对接合作,选择MBO、曼罗兰、莱默尔E+L、界龙集团、大连盛道等企业作为对象进行跨案例研究。研究明确了中德印刷产业供应链合作的框架与合作方式,分别确定出了纵向合作结构与横向合作结构下各自对接合作关键成功因素。

关键词：供应链对接与合作跨国合作印刷产业关键成功因素

旧貌·新颜——荷兰马斯特里赫特多明我会Selexyz书店

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照明设计 2008年第1期 108-113页

作者： g.Weiss roos Aldershoff（摄影）

荷兰Selexyz书店位于一座古老的多明我会（Dominican）教堂内，该教堂是一片巨大修道院的部分。它所在的城市马斯特里赫特（Maastricht）与比利时、德国毗邻，有着悠久而独特的历史，是荷兰南部重要的天主教教区，拥有许多古老的教堂，... 详细信息

荷兰Selexyz书店位于一座古老的多明我会（Dominican）教堂内，该教堂是一片巨大修道院的部分。它所在的城市马斯特里赫特（Maastricht）与比利时、德国毗邻，有着悠久而独特的历史，是荷兰南部重要的天主教教区，拥有许多古老的教堂，它们中的多数现在仍然在使用中，其中包括建于公元550年的荷兰第一个罗马教堂一圣瑟法斯大教堂（Saint Servatius Basilica）。从1794年起，也就是”法国时期（French period）”，马斯特里赫特被入侵的法国人占领，他们没收了很多宗教财产，并将它们变为军用。

关键词：荷兰书店法国人教堂修道院比利时天主教

Knowledge about HIV and AIDS among Young Women

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Open Journal of Nursing 2015年第6期5卷 558-565页

作者： Catherine M. Ngoma Janetta roos Seter Siziya Department of Nursing Sciences School of Medicine University of Zambia Lusaka Zambia Department of Health Studies University of South Africa Pretoria South Africa Department of Community Medicine School of Medicine University of Zambia Lusaka Zambia

HIV and AIDS are a major health concern among women worldwide. It is important that women especially youths are educated about HIV and AIDS because they represent a window of opportunity for reversing HIV rates if effective prevention programs can reach them before they engage in risky sexual behaviour. Women are more vulnerable to HIV infection than men and play a central role in the concept of the family, in nurturing, protecting, and caring for the family. The aim of the study was to explore HIV and AIDS knowledge among young women. A qualitative exploratory approach using focus group discussions was used and content analysis was used to interpret the data. A total of 8 focus group discussions were conducted two study sites intervention and control site. The analysis revealed 6 themes namely definition of HIV and AIDS, seriousness of HIV and AIDS in the community, signs and symptoms, transmission of HIV, cure for HIV and AIDS and prevention. The findings showed that some participants had knowledge deficit on HIV and AIDS, therefore continued community sensitisation is essential.

关键词： Knowledge HIV AIDS Young Women