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美国临床生化科学院检验医学实践指南:睾丸、前列腺、结直肠、乳腺及卵巢癌肿瘤标志物的应用

journal of Cancer Therapy（癌症治疗） 2014年第14期

作者： Caroline Brenner Larsen jan Lindebjerg Rikke Fredslund Andersen Niels Pallisgaard Susanne Holck Anders jakobsen hans jørgen nielsen

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临床检验杂志 2012年第2期30卷 116-160页

作者：鄢盛恺 Ulf-Hkan Stenman Rolf Lamerz Leendert H.Looijenga Nils Brünner Michael j.Duffy Caj Haglund Mads Holten-Andersen hans jфrgen nielsen Francisco j.Esteva Nadia Harbeck Daniel F.Hayes Rafael Molina Daniel W.Chan Robert C.Bast jr Ie-Ming Shih Lori j.Sokoll Gyrgy Slétormos 刘洋梁红艳姜晓峰田亚平薛丽林文涛顾兵潘世扬沈文梅郑磊李江卫生部中日友好医院检验科北京100029 北京豪迈生物工程有限公司北京100028

经美国临床生化科学院(NCAB)授权,由鄢盛恺教授组织学者、专家翻译审定2009年NCAB检验医学实践指南:睾丸、前列腺、结直肠、乳腺及卵巢癌中肿瘤标志物的应用单行本(Catharine ***教授和Eleftherios ***-dis教授编辑)。该指南包含第1至6... 详细信息

经美国临床生化科学院(NCAB)授权,由鄢盛恺教授组织学者、专家翻译审定2009年NCAB检验医学实践指南:睾丸、前列腺、结直肠、乳腺及卵巢癌中肿瘤标志物的应用单行本(Catharine ***教授和Eleftherios ***-dis教授编辑)。该指南包含第1至6章共6部分内容,参加指南制订的专家阵容强大,分别从临床及检验角度对睾丸、前列腺、结直肠、乳腺及卵巢癌中肿瘤标志物的检测与临床应用方面的问题进行了详细阐述与说明,是目前国内外在肿瘤实验诊断方面的纲领性应用文件。不仅适合广大检验人员、临床医护人员学习使用,也非常适合相关仪器试剂生产厂商研发应用。本指南由刘洋、薛丽、林文涛、李江、梁红艳、顾兵、潘世扬、郑磊等翻译,姜晓峰、沈文梅、田亚平、鄢盛恺审校,最后由鄢盛恺统稿审定。本指南的翻译出版,不仅有助于我国检验人员和医护人员合理的检测和应用睾丸、前列腺、结直肠、乳腺及卵巢癌肿瘤标志物,对我国检验医学指南的编写与应用也有一定的借鉴作用。本刊特在本期刊发,以飨广大读者。

关键词：肿瘤标志物临床应用实践指南检验医学临床生化前列腺结直肠卵巢癌

Effects of a novel bacterial phytase expressed in Aspergillus Oryzae on digestibility of calcium and phosphorus in diets fed to weanling or growing pigs

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journal of Animal Science and Biotechnology 2013年第2期4卷 102-111页

作者： Ferdinando nielsen Almeida Rommel Casilda Sulabo hans Henrik Stein Department of Animal Sciences University of Illinois

In 2 experiments, 48 weanling （initial BW： 13.5 ± 2.4 kg, Exp. 1） were used to determine effects of a novel bacterial 6-phytase and 24 growing pigs （initial BW： 36.2 ± 4.0 kg, Exp. 2） expressed in Aspergillus oryzae on the apparent total tract digestibility （ATTD） of phosphorus and calcium in corn-soybean meal diets fed to weanling and growing pigs. In Exp. 1 and 2, pigs were randomly allotted to 6 dietary treatments using a randomized complete block design and a balanced 2 period changeover design, respectively. In both experiments, 6 diets were formulated. The positive control diet was a corn-soybean meal diet with added inorganic phosphorus （Exp. 1：0.42 and 0.86% standardized total tract digestible phosphorus and total calcium, respectively; Exp. 2：0.32 and 0.79% standardized total tract digestible phosphorus and total calcium, respectively）. A negative control diet and 4 diets with the novel phytase （Ronozyme HiPhos, DSM Nutritional Products Inc., Parsippany, N j） added to the negative control diet at levels of 500, 1,000, 2,000, and 4,000 phytase units （FYT）/kg were also formulated. In Exp. 1, the ATTD of phosphorus was greater （P 〈 0.01） for the positive control diet （60.5%） than for the negative control diet （40.5%）, but increased （linear and quadratic, P 〈 0.01） as phytase was added to the negative control diet （40.5% vs. 61.6%, 65.1%, 68.7%, and 68.0%）. The breakpoint for the ATTD of phosphorus （68.4%） was reached at a phytase inclusion level of 1,016 FYT/kg. In Exp. 2, the ATTD of phosphorus was greater （P〈 0.01） for the positive control diet （59.4%） than for the negative control diet （39.8%） and increased （linear and quadratic, P〈 0.01） as phytase was added to the negative control diet （39.8% vs. 58.1%, 65.4%, 69.1%, and 72.8%）. The breakpoint for the ATTD of phosphorus （69.1%） was reached at a phytase inclusion level of 801 FYT/kg. in conclusion, the novel bacterial 6-phytase improved the ATTD of phosphorus and calcium in both weanling and growing pigs.

关键词： Calcium Digestibility Phosphorus Phytase Pigs

Experimental human pain models in gastro-esophageal reflux disease and unexplained chest pain

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World journal of Gastroenterology 2006年第18期12卷 2805-2817页

作者： Asbjφrn Mohr Drewes Lars Arendt-nielsen Peter Funch-jensen hans Gregersen Center for Visceral Biomechanics and Pain Department of GastroenterologyAalborg Hospitaland Center for Sensory-Motor InteractionsDepartment of Health Science and TechnologyAalborg UniversityDenmark Department of Surgical Gastroenterology L Aarhus HospitalDenmark

Methods related to experimental human pain research aim at activating different nociceptors, evoke pain from different organs and activate specific pathways and mechanisms. The different possibilities for using mechanical, electrical, thermal and chemical methods in visceral pain research are discussed with emphasis of combinations (e.g., the multimodal approach). The methods have been used widely in assessment of pain mechanisms in the esophagus and have contributed to our understanding of the symptoms reported in these patients. Hence abnormal activation and plastic changes of central pain pathways seem to play a major role in the symptoms in some patients with gastro-esophageal reflux disease and in patients with functional chest pain of esophageal origin. These findings may lead to an alternative approach for treatment in patients that does not respond to conventional medical or surgical therapy.

关键词： Pain Gut Experimental Chest pain Esophagitis

Amino acid digestibility of heat damaged distillers dried grains with solubles fed to pigs

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journal of Animal Science and Biotechnology 2014年第1期5卷 88-97页

作者： Ferdinando nielsen Almeida john Kyaw Htoo john Thomson hans Henrik Stein Department of Animal Sciences University of Illinois1207 W Gregory DrUrbana IL 61801 USA Evonik Industries AG Rodenbacher Chaussee 4Hanau 63457 Germany Evonik Degussa Corporation 1701 Barrett LakesBlvd NW Kennesaw GA 30144 USA

The primary objective of this experiment was to determine the effects of heat treatment on the standardized ileal digestibility （SID） of amino acids （AA） in corn distillers dried grains with solubles （DDGS） fed to growing pigs. The second objective was to develop regression equations that may be used to predict the concentration of SID AA in corn DDGS. A source of corn DDGS was divided into 4 batches that were either not autoclaved or autoclaved at 130℃ for 10, 20, or 30 min. Four diets containing DDGS from each of the 4 batches were formulated with DDGS being the only source of AA and CP in the diets. A N-free diet also was formulated and used to determine the basal endogenous losses of CP and AA. Ten growing pigs （initial BW： 53.5 ＋ 3.9 kg） were surgically equipped with a T-cannula in the distal ileum and allotted to a replicated 5 x4 Youden square design with 5 diets and 4 periods in each square. The SID of CP decreased linearly （P〈 0.05） from 77.9% in non-autoclaved DDGS to 72.1, 66.1, and 68.5% in the DDGS samples that were autoclaved for 10, 20, or 30 min, respectively. The SID of lysine was quadratically reduced （P〈 0.05） from 66.8% in the non-autoclaved DDGS to 54.9, 55.3, and 51.9% in the DDGS autoclaved for 10, 20, or 30 min, respectively. The concentrations of SID Arginine, Histidine, Leucine, Lysine, Methionine, Phenylalanine, or Threonine may be best predicted by equations that include the concentration of acid detergent insoluble N in the model （r2 = 0.76, 0.68, 0.67, 0.84, 0.76, 0.73, or 0.54, respectively）. The concentrations of SID Isoleucine and Valine were predicted （r2 = 0.58 and 0.54, respectively） by the Lysine：CP ratio, whereas the concentration of SID Tryptophan was predicted （r2 = 0.70） by the analyzed concentration of Tryptophan in DDGS. In conclusion, the SID of AA is decreased as a result of heat damage and the concentration of SID AA in heat-damaged DDGS may be predicted by regression equations developed in this experiment.

关键词： Amino acids Digestibility Distillers dried grains with solubles Heat damage

Amino acid digestibility in low-fat distillers dried grains with solubles fed to growing pigs

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journal of Animal Science and Biotechnology 2014年第4期5卷 414-420页

作者： Shelby Marie Curry Diego Mario David Labadan Navarro Ferdinando nielsen Almeida juliana Abranches Soares Almeida hans Henrik Stein Department of Animal Sciences University of Illinois

The objective of this experiment was to determine the standardized ileal digestibility（SID） of amino acids（AA） in 3sources of distillers dried grains with solubles（DDGS） with different concentrations of *** growing barrows（initial body weight：76.1 ± 6.2 kg） were randomly allotted to a replicated 6×4 Youden square design with 6 diets and 4 *** fat content of the 3 sources of DDGS were 11.5,7.5,and 6.9%*** contained 60%DDGS and fat concentration of the diets were 7.5,52,and 5.2%,*** additional diets containing the 2sources of DDGS with 75 and 6.9%fat were also formulated,and corn oil was added to these diets to increase the concentration of fat in the diets to levels that were calculated to be similar to the diet containing conventional DDGS with 11.5%fat.A N-free diet was also formulated to calculate endogenous losses of crude protein（CP） and AA from the *** were fed experimental diets during four 7-d *** first 5 d of each period were an adaptation period and ileal digesta were collected on d 6 and 7 of each *** apparent ileal digestibililty（AID） and SID of CP and all indispensable AA,except AID Pro and SID of Trp,were greater（P 〈 0.01） in conventional DDGS than in the 2 sources of DDGS with reduced *** oil to the diets containing the 2 sources of DDGS with reduced fat did not consistently increase SID of *** conclusion,conventional DDGS has greater SID values for most AA compared with DDGS that contains less fat and inclusion of additional oil to diets containing low-fat DDGS does not increase AID or SID of *** lower AA digestibility in low-fat DDGS could not be overcome by the inclusion of additional fat to the diets.

关键词： Amino acid digestibility Distillers dried grains with solubles Pigs

Front line defenders of the ecological niche!Screening the structural diversity of peptaibiotics from saprotrophic and fungicolous Trichoderma/Hypocrea species

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Fungal Diversity 2014年第6期69卷 117-146页

作者： Christian R.Rohrich Walter M.jaklitsch Hermann Voglmayr Anita Iversen Andreas Vilcinskas Kristian Fog nielsen Ulf Thrane hans von Dohren hans Bruckner Thomas Degenkolb Bioresources Project Group Fraunhofer Institute for Molecular Biology and Applied Ecology(IME)Winchesterstrasse 235394 GiessenGermany Department of Systematic and Evolutionary Botany Faculty Centre of BiodiversityUniversity of ViennaRennweg 141030 ViennaAustria Department of Systems Biology Technical University of DenmarkSoltofts PladsBuilding 2212800 Kgs.LyngbyDenmark Interdisciplinary Research Centre for BioSystems Land Use and Nutrition(IFZ)Department of Applied EntomologyInstitute of Phytopathology and Applied Zoology(IPAZ)University of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany Biochemistry and Molecular Biology OE 2 Institute of ChemistryTechnical University of BerlinFranklinstrasse 2910587 BerlinGermany Interdisciplinary Research Centre for BioSystems Land Use and Nutrition(IFZ)Department of Food SciencesInstitute ofNutritional ScienceUniversity of GiessenHeinrich-Buff-Ring 26-3235392 GiessenGermany AB SCIEXGermany GmbH Landwehrstrasse 5464293 DarmstadtGermany Danish Emergency Management Agency Universitetsparken 22100 CopenhagenDenmark

Approximately 950 individual sequences of nonribosomally biosynthesised peptides are produced by the genus Trichoderma/Hypocreathat belong to a perpetually growing class of mostly linear antibiotic oligopeptides,which are rich in the non-proteinogenic α-aminoisobutyric acid(Aib).Thus,they are comprehensively named ***,peptaibiotics represent ca.80% of the total inventory of secondarymetabolites currently known from Trichoderma/*** unique membrane-modifying bioactivity results from amphipathicity and helicity,thus making them ideal candidates in assisting both colonisation and defence of the natural habitats by their fungal *** this,reports on the in vivo-detection of peptaibiotics have scarcely been published in the *** order to evaluate the significance of peptaibiotic production for a broader range of potential producers,we screened nine specimens belonging to seven hitherto uninvestigated fungicolous or saprotrophic Trichoderma/Hypocrea species by liquid chromatography coupled to electrospray high resolution mass *** of peptaibiotics found were independently confirmed by analysing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the *** the nine species examined,five were screened positive for peptaibiotics.A total of 78 peptaibiotics were sequenced,56(=72%)of which are ***,dihydroxyphenylalaninol and O-prenylated tyrosinol,two C-terminal residues,which have not been reported for peptaibiotics before,were found as well as new and recurrent sequences carrying the recently described tyrosinol residue at their *** majority of peptaibiotics sequenced are 18-or 19-residue *** homologies with‘classical representatives’of subfamily 1(SF1)-peptaibiotics argue for the formation of transmembrane ion channels,which are prone to facilitate the producer capture and defence of its substratum.

关键词： HPLC/QTOF-ESI-HRMS Metabolite profiling Peptaibiotics Peptaibols Aib peptides Trichoderma Hypocrea

Antibody administration in experimental influenza increases survival and enhances the effect of oseltamivir

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Health 2012年第10期4卷 933-940页

作者： Brit N.j.Pourroy hans jorn Kolmos Lars P.nielsen Department of Virology Statens Serum InstituteCopenhagenDenmark Research Unite of Clinical Microbiology Institute of Clinical ResearchUniversity of Southern DenmarkOdenseDenmark

Anti-viral chemotherapy plays an important part in treating and preventing influenza illness. However, its effectiveness in severe infections can be debated and a reoccurring problem is the emergence of resistant virus. Passive immunisation has for a long time been and is still used for prophylaxis and treatment of a number of infectious diseases. In this experimental study anti-influenza antibodies were passively administrated to mice, subsequently they were infected with influenza virus and treated with oseltamivir. The aim was to investigate, if anti-influenza antibodies influenced the out come of oseltamivir treatment and development of resistance towards oseltamivir. We show, that oseltamivir alone was not able to effectively prevent a fatal outcome, but that oseltamivir administered together with a limited amount of antibodies, resulted in improvement of the clinical condition of the mice. The results also showed that a higher dosage of antibodies alone were able to protect the mice from a lethal dose of virus. These findings suggest that the effectiveness of oseltamivir depends on the host’s immune response to the influenza virus, and that that passive immunization is an option that should be considered in the in control of influenza.

关键词： Experimental Influenza Passively Administrated Antibodies Convalescent Plasma Passive Immunisation Oseltamivir Lethal Infection Polyclonal IgG Pandemic Control

Ultrasonographic study of mechanosensory properties in human esophagus during mechanical distension

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World journal of Gastroenterology 2006年第28期12卷 4517-4523页

作者： Ejnar Larsen Hariprasad Reddy AsbjΦrn Mohr Drewes Lars Arendt-nielsen hans Gregersen Department of Radiology Aalborg HospitalAalborg DK-9100Denmark Center for Visceral Biomechanics and Pain Aalborg HospitalAalborg DK-9100Denmark Laboratory for Experimental Pain Research Center for Sensory-Motor InteractionDepartment of Health TechnologyAalborg UniversityAalborg Dk-9100Denmark

TIM： To study the esophageal geometry and mechanosensation using endoscopic ultrasonography during volume-controlled ramp distensions in the distal esophagus. METHODS： Twelve healthy volunteers underwent distension of a bag. During distension up to moderate pain the sensory intensity was assessed on a visual analogue scale （VAS）. The esophageal deformation in terms of multidimensional stretch ratios and strains was calculated at different volumes and VAS levels. Distensions were done before and during administration of the anticholinergic drug butylscopolamine. RESULTS： The stimulus-response （volume-VAS） curve did not differ without or with the administration of butylscopolamine. Analysis of stretch ratios demonstrated tensile stretch in circumferential direction, compression in radial direction and a small tensile stretch in longitudinal direction. A strain gradient existed throughout the esophageal wall with the largest circumferential deformation at the mucosal surface. The sensation intensity increased exponentially as function of the strains. CONCLUSION： The method provides information of esophageal deformation gradients that correlate to the sensation intensity. Hence, it can be used to study mechanosensation in the human esophagus. Further studies are needed to determine the exact deformation stimulus for the esophageal mechanoreceptors.

关键词： Deformation analysis Esophagus Experimental pain Ultrasonography

高大冷杉温室Strophosoma象甲的微生物防治(英文)

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安徽农业大学学报 2007年第2期34卷 185-194页

作者：夏洛蒂.尼尔森 Susanne Vestergaard Susanne Harding jФrgen Eilenberg Department of Ecology Copenhagen UniversityFaculty of Life SciencesThorvaldsensvej 401871 Frederiksberg CDenmark

In Denmark,the weevils Strophosoma melanogrammum and *** cause economic damage in Noble fir due to the adult stage feeding on the *** chemical treatments of these weevils are allowed in Denmark,so biological control is an attractive *** evaluated the potential for microbial control of larvae of Strophosoma *** on laboratory bioassays and field applications,taking effect on both target and non-target into consideration,as well as persistence of the applied *** the laboratory Beauveria bassiana,Paecilomyces farinosus and Metarhizium anisopliae were able to infect and cause mycosis in Strophosoma *** the tested isolates the most virulent isolate was *** BIPESCO 5,which resulted in 80 % *** the field experiment ***,isolate BIPESCO 5,was applied to the soil as a conidial suspension against larvae of Strophosoma *** effect of the fungus on the target population was monitored at a weekly basis by counts of emerging adult weevils during their activity *** population of Strophosoma *** reduced by up to 60% in treated plots compared to control *** non-target effects of *** were studied by sampling insects and ticks from both treated and control *** days after treatment,two sampled insect orders(Hemiptera and Coleoptera)and ticks were found with prevalences of *** above 50%,compared to no infection in the insects collected from control *** in coccinellids were found as long as 277 days after ***,the effect on population level of non-target is still *** persistence of the fungus was documented by plating a soil suspension onto *** documented that conidia of *** could persist in the greenery plantation for at least 418 days after application.

关键词：高大冷杉温室微生物 Strophosoma象甲防治措施