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The genome-wide landscape of small insertion and deletion mutations in Monopterus albus

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Journal of Genetics and Genomics 2019年第2期46卷 75-86页

作者： Feng Chen Fengling Lai Majing Luo Yu-San Han hanhua cheng Rongjia Zhou Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Wuhan University Institute of Fisheries Science College of Life Science "National Taiwan University"

Insertion and deletion(indel) mutations, which can trigger single nucleotide substitutions on the flanking regions of genes, may generate abundant materials for disease defense, reproduction, species survival and evolution. However, genetic and evolutionary mechanisms of indels remain elusive. We establish a comparative genome-transcriptome-alignment approach for a large-scale identification of indels in Monopterus population. Over 2000 indels in 1738 indel genes, including 1-21 bp deletions and 1-15 bp insertions, were detected. Each indel gene had ～1.1 deletions/insertions, and 2-4 alleles in population. Frequencies of deletions were prominently higher than those of insertions on both genome and population levels. Most of the indels led to in frame mutations with multiples of three and majorly occurred in non-domain regions, indicating functional constraint or tolerance of the indels. All indel genes showed higher expression levels than non-indel genes during sex reversal. Slide window analysis of global expression levels in gonads showed a significant positive correlation with indel density in the genome. Moreover, indel genes were evolutionarily conserved and evolved slowly compared to nonindel genes. Notably, population genetic structure of indels revealed divergent evolution of Monopterus population, as bottleneck effect of biogeographic isolation by Taiwan Strait, China.

关键词： Sex determination Insertions Deletions Evolution Reproduction

Evolutionary conservation of Dmrt gene family in amphibi-ans, reptiles and birds

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Chinese Science Bulletin 2001年第23期46卷 1992-1995页

作者： REN Lili cheng hanhua GUO Yiqing HUANG Xiao LIU Li ZHOU Rongjia Department of Genetics and Center for Developmental Biology College of Life SciencesWuhan UniversityWuhan 430072China

Sex determining gene Mab-3 of C. elegans and doublesex of Drosophila contain a common DNA binding motif called a DM domain, both of which regulate similar aspects of sexual development. Human doublesex-related gene DMRT1 has been identified, which also contains the conserved DM-related DNA-binding domain and plays an essential role in gonadal differentiation. We present the amplification of a broad spectrum of DM domain sequences from phylogenetic diverse vertebrates (Cynops orientalis, Chrysemys scripta elegans and Coturnix coturnix) using degenerate PCR. Our results further reveal the unexpected complexity and the evolutionary conservation of the DM domain gene family.

关键词： DM domain degenerate PCR gene family phylogenet- ics.

Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish

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Protein & Cell 2019年第5期10卷 347-364页

作者： Qiang Hong Cong Li Ruhong Ying Heming Lin Jingqiu Li Yu Zhao hanhua cheng Rongjia Zhou Hubei Key Laboratory of Cell Homeostasis College of Life SciencesWuhan UniversityWuhan 430072China

Folliculogenesis is essential for production of female gametes in ***,the molecular mechanisms underlying follicle development,particularly apoptosis regulation in ovary,remain ***,we gen erated sox3 knockout zebrafish lines using CRISPR/***3 knockout led to follicle development retardation and a reduced fecundity in *** analysis of transcriptome between sox3^-/-and wild-type ovaries revealed that Sox3 was invoIved in pathways of ovarian steroidogenesis and *** of sox3 promoted follicle apoptosis and obvious apoptosis signals were detected in somatic cells of stages III and IV follicles of sox3^-/-***,Sox3 can bind to and activate the promoter of ***-regulation of Cyp19a1a expression promoted 17β-estradiol synthesis,which inhibited apoptosis in follicle ***,Sox3 functions as a regulator of Cyp19a1a expression,via 17β-E2 linking apoptosis suppression,which is implicated in improving female fecundity.

关键词： Sox3 follicle development apoptosis Cyp19a1a zebrafish

Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells

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Long-term artificial selection reveals a role of TCTP in aut...

第三届“模式生物与人类健康”发育遗传学全国学术研讨会

作者： Ke Chen Chunhua Huang Jia Yuan hanhua cheng 周荣家 Department of Genetics College of Life SciencesWuhan University

Understanding genomic variation and detecting selection signatures in a genome under selection have been great challenges for a *** mammalian ovary,activation,development/exhaustion of primordial follicles determines reproductive success,menopause/end of female reproductive ***,molecular mechanisms underlying oogenesis,particularly under artificial selection,are largely *** report that a proteome-wide scan for selection signatures

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The small GTPase RAB37 promotesautophagosome formationby recruiting ATG5-12

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The small GTPase RAB37 promotesautophagosome formationby rec...

2015中国遗传学会大会

作者： Yue Sheng Ying Song Jia Yuan Yibin cheng Chunhua Huang MajingLuo Zhigang Li hanhua cheng Rongjia Zhou Department of Genetics College of Life SciencesWuhan University

lntracellular membrane trafficking is essential for eukaryotic cell ***,we show that RAB37,a small GTPase involved in vesicle transport,is a target of ATG5,a key protein required for ***37activation through GTP binding recruits ATG5-12 to isolation membrane and promotes autophagosome *** analysis reveals that GTP-bound RAB37 exhibits enhanced interactions with ATG5-12 and GDP-stabilized mutation impairs the ***37 recruits ATG5-12 to LC3-Ⅱpuncta in a GTP-dependent manner to promote ***,RAB37 vesicles participate in formation of autophagosomes and subsequent autolysosomesupon starvation *** ablation of RAB37 expression decreases autophagy and increases tumour metastasis in vivo,whereas forced RAB37 expression suppresses cell proliferation and tumour *** results identify RAB37 as a master organiser of vesicle transport and autophagosome formation through a molecular link of RAB37,ATG5-12 up to *** findings have broad implications for understanding me role of RAB37 vesicle transport in autophagy and cancer.

关键词： Vesicle transport RAB37 ATG5 Autophagy Cancer

Correction To:Germline stem cells in human

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Signal Transduction and Targeted Therapy 2023年第1期8卷 314-314页

作者： hanhua cheng Dantong Shang Rongjia Zhou Hubei Key Laboratory of Cell Homeostasis College of Life Sciences Renmin Hospital of Wuhan University Wuhan University 430072 Wuhan China

Correction to:Signal Transduction and Targeted Therapy https://***/10.1038/s41392-022-01197-3,published online 02 October 2022 In this article1,the legend in Fig.3 has a typo,“Extraembryonic”inadvertently supplied a... 详细信息

关键词： Therapy figure Correction

Insights into autophagosome biogenesis during oogenesis

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Insights into autophagosome biogenesis during oogenesis

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Jia Yuan Yue Sheng Ying Song Ying Zhang hanhua cheng Rongjia Zhou Hubei Key Laboratory of Cell Homeostasis College of Life SciencesWuhan University

In the mammalian ovary, folliculogenesis begins from primordial germ cells, proceeds through the primary follicle stage to the mature follicle stage and finally produces functional oocytes. The molecular mechanisms of folliculogenesis are not completely understood yet. Identification of the key molecular events that control follicular development will help us to understand ovarian functions. In this study, we investigate autophagic roles in oogenesis. In an attempt to better understand the functions and molecular mechanisms of RAB37 in autophagy, we explore ATG proteins interacting with RAB37 and find that RAB37 as a key organiser of autophagosome formation promotes autophagy through the ATG5-ATG12-ATG16 L1 complex. It binds directly with ATG5, and promotes autophagosome formation in a GTP-dependent manner. RAB37 mutation and knockdown can impair autophagy in vivo. We discuss autophgic roles in folliculogenesis. The study answers the key, basic, and long-standing question of autophagosome biogenesis, and proposes that RAB37 acts as a key organizer of autophagosomal membrane biogenesis. The molecular mechanism for autophagosome biogenesis has broad physiological and pathological implications for understanding the autophagy regulation during ovary development.

关键词： follicular development autophagy ovary

Transfer RNAs Evolve through Retrotransposition

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Transfer RNAs Evolve through Retrotransposition

湖北省遗传学会第八次代表大会暨学术讨论会

作者： Zhixiang Zuo,hanhua cheng and Rongjia Zhou College of Life Sciences,Wuhan University,Wuhan 430072

Transfer RNAs(tRNAs) play an important role as the linkage between mRNA and amino acids during protein *** studies have revealed many split tRNAs which are encoded by two or more separate *** discovery of split tRNAs induces a heat debate on the evolutionary order of these split tRNAs and the continuous tRNAs which are major members in modern *** this paper,we conduct a deep sequence analysis on the tRNA genes and nearby regions,which cover all the three life *** show that single nucleotide substitution rate increases close to tRNA genes and this pattern is conserved among all the three life domains including archaeas,bacterias and eukaryotes as indels *** is strongly indicated that tRNA genes are insertion-derived *** half-tRNA-like sequences are universally found in many different organisms and these half-tRNA-like sequences are proved to be an ancient compared to modern tRNA ***,most of the half-tRNA-like sequences locate in retrotransposon regions of the corresponding *** we experimentally validate the expression of the half-tRNA-like sequences in the mouse brain tissues using *** results indicate that modern tRNA genes evolve from split tRNA genes through retrotransposition.

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通识教育中融入人类遗传学的教学实践

遗传 2020年第2期42卷 222-229页

作者：程汉华周荣家武汉大学生命科学学院

通识教育已经成为大学高等教育的重要组成部分,强调通专结合的办学理念,要求践行“以人为本”的教育实践。在教学实践中存在不少困难和困惑,需要不断总结和发展中国特色的通识教育体系。本文凝练了在人类生物学教学实践中如何融入人类... 详细信息

通识教育已经成为大学高等教育的重要组成部分,强调通专结合的办学理念,要求践行“以人为本”的教育实践。在教学实践中存在不少困难和困惑,需要不断总结和发展中国特色的通识教育体系。本文凝练了在人类生物学教学实践中如何融入人类遗传学知识的通识教学理念、案例和分析,以“人是什么”为切入点引入贴近生活的教学内容。通过现代进化基因组学知识,解析人类自身发展的过去、现在和未来所涉及的生物学核心问题。剖析人的经典属性及其与通识教育的特殊关联,进而引出基于问题的深层次思考。

关键词：通识教育人类生物学遗传学