检索结果-南通市图书馆

维普期刊数据库博看期刊

Experimental study on the shear behavior of the interface between cushion materials and the concrete raft

在线全文

学校读者我要写书评

暂无评论

Earthquake Engineering and Engineering Vibration 2018年第1期17卷 165-178页

作者： li Yaokun han Xiaolei Khaled Galal Ji Jing State key Laboratory of Subtropical Building Science South China University of Technology Department of Building Civil and Environmental EngineeringConcordia University

Cushion is a layer of granular materials between the raft and the ground. The shear behavior of the interface between the cushion and the raft may influence the seismic performance of the superstructure. In order to quantify such influences, horizontal shear tests on the interfaces between different cushion materials and concrete raft under monotonic and cyclic loading were carried out. The vertical pressure P_v, material type and cushion thickness h_c were taken as variables. Conclusions include： 1） under monotonic loading, P_v is the most significant factor; the shear resistance P_（hmax） increases as P_v increases, but the normalized factor of resistance μ_n has an opposite tendency; 2） for the materials used in this study, μ_n varies from 0.40 to 0.70, the interface friction angle δ_s varies from 20° to 35°, while u_（max） varies from 3 mm to 15 mm; 3） under cyclic loading, the interface behavior can be abstracted as a ＂three-segment＂ back-bone curve, the main parameters include μ_n, the displacement u_1 and stiffness K_1 of the elastic stage, the displacement u_2 and stiffness K_2 of the plastic stage; 4） by observation and statistical analysis, the significance of different factors, together with values of K_1, K_2 and μ_n have been obtained.

关键词： cushion/raft interface shear behavior monotonic test cyclic test "three-segment" back-bone curve

HAIC联合动脉灌注榄香烯注射液治疗进展期肝癌临床研究

在线全文

学校读者我要写书评

暂无评论

海南医学 2024年第16期35卷 2301-2304页

作者：丁可徐庆余伟冰杨振华李叶枚韩菲菲江门市五邑中医院肿瘤科广东江门529000

目的探讨肝动脉灌注化疗术(HAIC)联合动脉灌注榄香烯注射液治疗进展期肝癌患者的临床疗效。方法选取2021年4月至2022年1月江门市五邑中医院收治的80例进展期肝癌患者展开研究,按随机数表法分为观察组和对照组各40例。两组患者均接受HAI... 详细信息

目的探讨肝动脉灌注化疗术(HAIC)联合动脉灌注榄香烯注射液治疗进展期肝癌患者的临床疗效。方法选取2021年4月至2022年1月江门市五邑中医院收治的80例进展期肝癌患者展开研究,按随机数表法分为观察组和对照组各40例。两组患者均接受HAIC治疗,对照组患者药物为奥沙利铂、氟尿嘧啶,观察组患者则在对照组治疗的基础上联合榄香烯注射液动脉灌注,两组患者均以3周为一个治疗周期,连续治疗6周。比较两组患者治疗6周后的临床疗效,比较两组患者随访2年的总生存期(OS)、无进展生存期(PFS),同时比较两组患者治疗期间的不良反应发生率。结果观察组患者的客观缓解率(ORR)为47.50%,明显高于对照组的25.00%,差异有统计学意义(P0.05);随访2年显示,观察组患者的OS、PFS分别为(16.12±3.69)个月、(14.60±3.30)个月,明显长于对照组的(13.44±3.71)个月、(11.27±3.08)个月,差异均有统计学意义(P0.05)。结论HAIC联合动脉灌注榄香烯注射液治疗进展期肝癌可提高患者的ORR,延长OS、PFS,具有临床应用价值。

关键词：进展期肝癌肝动脉灌注化疗术榄香烯注射液客观缓解率总生存期无进展生存期

Enhancing gene editing efficiency for cells by CRISPR/Cas9 system-loaded multilayered nanoparticles assembled via microfluidics

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2021年第10期34卷 216-220页

作者： Xuanyu li Qiang Feng Ziwei han Xingyu Jiang Shenzhen Bay Laboratory Department of Biomedical EngineeringSouthern University of Science and TechnologyShenzhen 518055China

Most non-viral carriers for in vitro delivery of nucleic acids suffer from low efficiency of introducing m RNA and other nucleic acids,especially large m ***9 protein is the nuclease part of the powerful gene-editing tool,CRISPR/Cas9 system,Cas9 m RNA is particularly large,thus presents a big challenge for *** assembled a multilayered biodegradable nanocarrier to load Cas9 m RNA inside to protect Cas9 m RNA from *** used a microfluidic chip to synthesize a small,positively charged,and degradable core to attract negatively charged Cas9 m *** microfluidic assembly allows the core to be small enough to incorporate into a cationic *** multilayered nanocarriers elevated the delivery efficiency of Cas9 m RNA by over 2 folds and increased the expression by over 5 folds compared to commercially used non-viral *** addition,the multilayered nanocarriers do not require reduced serum medium for *** using the standard complete medium for transfection,the multilayered nanocarriers could increase the expression of Cas9 m RNA by over 15 folds compared to commercially used non-viral *** co-delivery of Cas9 m RNA and sg RNA via LRC elevated the gene-editing efficiency by 3 folds compared to that via commercially used non-viral *** on the higher transfection efficiency of Cas9 m RNA/sg RNA than commercially used non-viral carriers,these multilayered nanocarriers may have a good prospect as efficient commercial delivery carriers for Cas9 m RNA/sg RNA and other nucleic acids.

关键词： Nanotechnology Biomedical engineering Microfluidics CRISPR/Cas9 system Poly(lactic-co-glycolic acid)

Exosomal miR-320e through wnt2targeted inhibition of the Wnt/β-catenin pathway allevisate cerebral small vessel disease and cognitive impairment

在线全文

学校读者我要写书评

暂无评论

世界精神病学杂志 2023年第9期13卷 630-644页

作者： Zheng Wang Xue-Ning li Shao-Nan Yang Yuan Wang ke-Jin Gao Bin han Ai-Jun Ma Department of Internal Medicine-Neurology Affiliated Hospital of Qingdao UniversityQingdao 266001Shandong ProvinceChina Department of Neurology The Affiliated Hospital of Qingdao UniversityQingdao 266001Shandong ProvinceChina

BACKGROUND Exosomal miRNAs play crucial roles in many central nervous system *** small vessel disease(CVSD)is a small vessel disease that is affected by various *** study aimed to investigate the role of exosomal miR-320e in the Wnt/β-catenin pathway stimulated by oxidative stress and assess its clinical correlation with psychiatric symptoms in patients with *** To explore whether exosomal miR-320e could suppress the Wnt/β-catenin pathway and play a protective role in CVSD progression,as well as examine its potential correlation with cognitive impairment and depression in patients with *** Differentially expressed exosomal miRNAs were filtered by sequencing plasma exosomes from patients with CVSD and healthy *** and dual luciferase analyses were used to confirm the binding of miR-320e to Wnt2,and the mRNA and protein levels of downstream components in the Wnt/β-catenin pathway were evaluated when overexpressed or with knockdown of miR-320e under H2O2-induced oxidative *** addition,Wnt2-targeting siRNA was used to confirm the role of miR-320e in the Wnt2-mediated inhibition of the Wnt/β-catenin pathway.A retrospective analysis was conducted among patients with CVSD to confirm the correlation between miR-320e expression and the severity of cognitive impairment and depression,which were quantified using the Montreal Cognitive Assessment(MoCA)/Executive Function Assessment(EFA),and the Hamilton Depression Scale(HAMD)/Beck Depression Inventory(BDI),*** High-throughput sequencing revealed that exosomal miR-320e was downregulated in patients with *** analysis and dual-luciferase reporter gene experiments showed that exosomal miR-320e inhibited the Wnt/β-catenin pathway in response to oxidative stress by targeting the 3'noncoding region of *** of exosomes carrying miR-320e into endothelial cells could also target Wnt2 and inhibit the Wnt2/β-catenin *** miR-32

关键词： Exosome Cerebral small vessel disease miRNA-320e Wnt2 Wnt/β-catenin pathway Depressed

不完全契约与公平交易:功能比较视域下债权人撤销权的解释性重构

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

河北法学 2024年第1期42卷 165-186页

作者：张兆函清华大学法学院北京100091

《民法典》施行后,债权人撤销权制度中债权人得否向相对人直接请求等基础问题仍存争议,其根源在于债权人撤销权中破产外撤销权的制度定位尚未被充分探讨。民法典评价“影响债权人的债权实现”,与英美法系发轫于欺诈转让法的可撤销交易... 详细信息

《民法典》施行后,债权人撤销权制度中债权人得否向相对人直接请求等基础问题仍存争议,其根源在于债权人撤销权中破产外撤销权的制度定位尚未被充分探讨。民法典评价“影响债权人的债权实现”,与英美法系发轫于欺诈转让法的可撤销交易制度以公平合理对价为规制中心的思路相合。债权人撤销权作为不完全契约的缺省规则,可分为打击低价(及无偿)转让侵害债权及偏颇清偿侵害债权两项子类型,它们有着部分相似的事实构成和相似的法律效果。《民法典》第538—542条有着高度弹性的解释空间,低价转让损害债权重在考察交易对债务人清偿能力的影响,偏颇清偿则重在诈害意图;“撤销”原则上宜解释为相对于债权人不生效力,且承认相对人及后手受让人基于支付公平合理对价的抗辩权以维护交易安全;破产外救济的定位决定了债权人应享有针对相对人的直接权。债权人撤销权作为规制财产不当减损行为的基础规则,值得充分发掘。

关键词：债权人撤销权破产外撤销相对无效欺诈转让偏颇清偿

纳米晶块体Co-45Ni-10Cr合金在H 2SO 4溶液中腐蚀电化学行为

在线全文

学校读者我要写书评

暂无评论

有色金属工程 2024年第1期14卷 1-8页

作者：王晴范馨月张昊鑫韩漾曹中秋张轲王艳辛士刚沈阳师范大学化学化工学院沈阳110034 沈阳师范大学实验教学中心沈阳110034

利用粉末冶金工艺制备常规尺寸(CS)Co-45Ni-10Cr合金粉末,再利用机械合金化法制备纳米尺寸(NS)Co-45Ni-10Cr合金粉末,采用真空热压技术得到相应的块体Co-45Ni-10Cr合金。腐蚀介质选择不同浓度的H_(2)SO_(4)溶液,利用电化学工作站分别测... 详细信息

利用粉末冶金工艺制备常规尺寸(CS)Co-45Ni-10Cr合金粉末,再利用机械合金化法制备纳米尺寸(NS)Co-45Ni-10Cr合金粉末,采用真空热压技术得到相应的块体Co-45Ni-10Cr合金。腐蚀介质选择不同浓度的H_(2)SO_(4)溶液,利用电化学工作站分别测试两种Co-45Ni-10Cr(CS和NS)块体合金的自腐蚀电位、动电位极化曲线和交流阻抗谱,对比研究了两者腐蚀行为以及晶粒细化对于它们腐蚀行为产生的影响。结果表明:两种Co-45Ni-10Cr(CS和NS)块体合金的自腐蚀电位均随着腐蚀介质H_(2)SO_(4)溶液浓度增大而发生负移,腐蚀倾向逐渐加剧,同时腐蚀电流密度均增大,电荷传递电阻相应减小,因此腐蚀速度加快。此外,两种Co-45Ni-10Cr(CS和NS)块体合金的交流阻抗谱均为单一容抗弧,说明腐蚀速率均由电化学过程控制。与Co-45Ni-10Cr(CS)合金相比,Co-45Ni-10Cr(NS)合金的腐蚀速率减慢,表明纳米化后Co-45Ni-10Cr块体合金的耐腐蚀性能有所提高。

关键词：腐蚀电化学块体纳米晶 Co-45Ni-10Cr合金机械合金化热压

Recent advances in Pb-Sn mixed perovskite solar cells

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2022年第10期31卷 615-638,I0015页

作者： Yanyu Deng Guanhua Ren Danao han Wenbin han Zhuowei li Chunyu liu Wenbin Guo State key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012JilinChina

Organic-inorganic hybrid lead-tin perovskite solar cells(Pb-Sn PSCs)have attracted much attention because of their advantages of low toxicity,variable bandgap,and feasibility for all-perovskite tandem solar cells,and the current power conversion efficiency(PCE)has exceeded 23%.However,due to the rambunctious crystallization process,easily oxidized Sn(Ⅱ)and inadequate energy level arrangement,there are many defects in perovskite films resulting in serious carrier recombination,which makes PCE still lag Pb-based *** quality of perovskite films is an important factor affecting the overall device *** selection and optimization of transport layers not only determines the interface energy level arrangement but also affects the carrier *** this paper,the research progress in improving performance of Pb-Sn PSCs in recent years is reviewed from aspects of perovskite layer and transport *** profound understanding of different promotion methods is summarized as *** results have certain guiding significance for the future development and commercial application of Pb-Sn PSCs.

关键词： Pb-Sn mixed perovskite solar cells Crystallinity Charge transport layer Passivation Additive

群体感应调控子AphA对河弧菌Ⅵ型分泌系统VflT6SS2的调控研究

在线全文

学校读者我要写书评

暂无评论

中华流行病学杂志 2024年第4期45卷 566-573页

作者：程倩韩雨黄元铭冀赛森李杰刁保卫梁未丽传染病溯源预警与智能决策全国重点实验室中国疾病预防控制中心传染病预防控制所北京102206

目的研究河弧菌群体感应调控子AphA对Ⅵ型分泌系统VflT6SS2功能活性的调控机制。方法采用Western Blot检测河弧菌野生株、aphA缺失株(ΔaphA)和aphA回补株中VflT6SS2标志性组件溶血素共调节蛋白(Hcp)的表达和分泌。采用实时荧光定量PCR... 详细信息

目的研究河弧菌群体感应调控子AphA对Ⅵ型分泌系统VflT6SS2功能活性的调控机制。方法采用Western Blot检测河弧菌野生株、aphA缺失株(ΔaphA)和aphA回补株中VflT6SS2标志性组件溶血素共调节蛋白(Hcp)的表达和分泌。采用实时荧光定量PCR和启动子-lux融合冷光系统检测野生株和ΔaphA中VflT6SS2核心基因簇和附属基因簇代表基因tssB2、hcp(tssD2)和vgrG(tssI2)以及群体感应调控子HapR的mRNA相对表达量和启动子活性。采用定点突变实验结合启动子活性测定确定AphA在tssD2b启动子区的调控结合位点;采用电泳迁移率位移测定(EMSA)确定AphA与hapR启动子的结合。结果ΔaphA中tssB2、hcp(tssD2)、vgrG(tssI2)和hapR的mRNA相对表达量及Hcp蛋白的表达分泌明显高于野生株。VflT6SS2核心基因簇、tssD2a、tssI2a和hapR的启动子活性在ΔaphA中均高于野生株,而tssD2b启动子活性则低于野生株。tssD2a和tssD2b的启动子序列分析显示-335 bp~-229 bp区差异较大,在tssD2b中该区域存在2个潜在AphA结合位点,将其中的保守位点ATG替换为CGA,tssD2b启动子活性明显降低。EMSA结果显示,AphA与hapR启动子直接结合。结论AphA在转录水平直接抑制hapR的表达,间接参与对VflT6SS2核心基因簇和附属基因簇的调控。AphA对tssD2a和tssD2b呈现相反的调控模式,AphA可直接与tssD2b启动子区(-335 bp~-229 bp)结合正向调控tssD2b的表达。

关键词：河弧菌群体感应调控子 Ⅵ型分泌系统转录调控

在线全文

学校读者我要写书评

暂无评论

葛根素靶向线粒体抗胶质瘤的机制

中国药理学通报 2024年第7期40卷 1317-1324页

作者：盛格格冯子瀚程聿德朱海丽陈绍恢湖北科技学院医学部药学院湖北咸宁437100 湖北科技学院医学部临床医学院湖北咸宁437100

目的探讨葛根素对胶质瘤细胞增殖、迁移和凋亡的影响及机制。方法GEO数据库分析胶质瘤与线粒体疾病共相关差异性表达基因;CCK-8检测细胞活力;划痕和Transwell检测细胞迁移;EdU检测细胞增殖;TUNEL检测细胞凋亡;Mito-Tracker检测线粒体膜... 详细信息

目的探讨葛根素对胶质瘤细胞增殖、迁移和凋亡的影响及机制。方法GEO数据库分析胶质瘤与线粒体疾病共相关差异性表达基因;CCK-8检测细胞活力;划痕和Transwell检测细胞迁移;EdU检测细胞增殖;TUNEL检测细胞凋亡;Mito-Tracker检测线粒体膜电位;ATP试剂盒分析ATP含量;免疫印迹分析蛋白质表达;皮下异种移植肿瘤模型分析葛根素抗肿瘤效果。结果胶质瘤和线粒体疾病共相关差异性表达基因178个;经GO和keGG富集及相互作用网络分析,筛选出12个核心基因;其中泛素蛋白C(ubiquitin C,UBC)在胶质瘤患者组织中高表达。葛根素可与UBC结合,并在动物和细胞水平降低UBC表达。葛根素处理可缩小胶质瘤体积,降低胶质瘤细胞增殖和迁移,促进胶质瘤细胞凋亡;同时降低线粒体膜电位和ATP含量,并下调UBC通路蛋白表达水平。结论葛根素抑制胶质瘤细胞增殖和迁移并促进其凋亡,其机制与诱发线粒体功能紊乱有关。

关键词：葛根素胶质瘤线粒体泛素蛋白C 网络药理学分子对接