Exosomal miR-320e through wnt2targeted inhibition of the Wnt/β-catenin pathway allevisate cerebral small vessel disease and cognitive impairment
作者机构：Department of Internal Medicine-NeurologyAffiliated Hospital of Qingdao UniversityQingdao 266001Shandong ProvinceChina Department of NeurologyThe Affiliated Hospital of Qingdao UniversityQingdao 266001Shandong ProvinceChina
出 版 物：《世界精神病学杂志》 (World Journal of Psychiatry)
年 卷 期：2023年第13卷第9期
页 面：630-644页
核心收录：
学科分类：1002[医学-临床医学] 100205[医学-精神病与精神卫生学] 10[医学]
主 题：Exosome Cerebral small vessel disease miRNA-320e Wnt2 Wnt/β-catenin pathway Depressed
摘 要：BACKGROUND Exosomal miRNAs play crucial roles in many central nervous system *** small vessel disease(CVSD)is a small vessel disease that is affected by various *** study aimed to investigate the role of exosomal miR-320e in the Wnt/β-catenin pathway stimulated by oxidative stress and assess its clinical correlation with psychiatric symptoms in patients with *** To explore whether exosomal miR-320e could suppress the Wnt/β-catenin pathway and play a protective role in CVSD progression,as well as examine its potential correlation with cognitive impairment and depression in patients with *** Differentially expressed exosomal miRNAs were filtered by sequencing plasma exosomes from patients with CVSD and healthy *** and dual luciferase analyses were used to confirm the binding of miR-320e to Wnt2,and the mRNA and protein levels of downstream components in the Wnt/β-catenin pathway were evaluated when overexpressed or with knockdown of miR-320e under H2O2-induced oxidative *** addition,Wnt2-targeting siRNA was used to confirm the role of miR-320e in the Wnt2-mediated inhibition of the Wnt/β-catenin pathway.A retrospective analysis was conducted among patients with CVSD to confirm the correlation between miR-320e expression and the severity of cognitive impairment and depression,which were quantified using the Montreal Cognitive Assessment(MoCA)/Executive Function Assessment(EFA),and the Hamilton Depression Scale(HAMD)/Beck Depression Inventory(BDI),*** High-throughput sequencing revealed that exosomal miR-320e was downregulated in patients with *** analysis and dual-luciferase reporter gene experiments showed that exosomal miR-320e inhibited the Wnt/β-catenin pathway in response to oxidative stress by targeting the 3 noncoding region of *** of exosomes carrying miR-320e into endothelial cells could also target Wnt2 and inhibit the Wnt2/β-catenin *** miR-32
维普期刊数据库