检索结果-南通市图书馆

Elevated serum prostaglandin E2 predicts the risk of infection in hepatitis B virus-related acute-on-chronic liver failure patients

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2017年第9期10卷 916-920页

作者： Xiao-Ping Huang Yan Wang Li Chen Wei Sun Yan Huang Ying Xu Ting-Ting feng Er-Ping Luo Ai-Lan Qin Wei-feng zhao Jian-He Gan Department of Infectious Diseases The First Affiliated Hospital of Suzhou UniversitySuzhouChina

Objective: To evaluate the serum Prostaglandin E2(PGE2) level in Acute-on-chronic liver failure(ACLF) and determine its predicative value for ***: From April 2014 to April 2015, ninety-one patients with hepatitis B virus and ACLF but without infection were enrolled into this prospective study that was carried out at our Hospital. Twenty patients with stable chronic hepatitis B were enrolled from the outpatient department and twenty healthy control subjects without any disease were enrolled from hospital staff. Serum PGE2 levels were determined using ELISA at enrollment. Clinical and laboratory parameters were collected. Receiver operating characteristic(ROC) curves were used to determine optimal cut-off values to predict ***: Significantly higher PGE2 levels were found in patients with ACLF in comparison with healthy controls and patients with stable CHB(P < 0.000 1). In ACLF patients, PGE2 levels were significantly higher in patients that eventually developed infection than those without this complication(P < 0.000 1). ROC analysis showed that serum PGE2(area under the ROC curve, 0.83) could predict infection in patients with ACLF with sensitivity of 78.4% and specificity of 81.5% using a threshold of 141 pg/m ***: Serum PGE2 is associated with the susceptibility to secondary infections for patients with ACLF. Increased PGE2 serum levels may serve as a potential biomarker for developing infections in ACLF patients.

关键词： Acute-on-chronic liver failure Immune paralysis Infection Prostaglandin E2

Co-B/γ-Al_2O_3非晶态催化剂的制备及其催化乳酸乙酯液相加氢性能

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

物理化学学报 2015年第3期31卷 527-532页

作者：赵凤伟沈美玉王琳杜长海长春工业大学化学工程学院长春130012

采用浸渍-还原法制备了负载型Co-B/γ-Al2O3非晶态合金催化剂,并将其应用于乳酸乙酯液相加氢制备1,2-丙二醇(1,2-PDO)反应中,研究了其催化加氢性能.采用电感耦合等离子体(ICP)发射光谱仪、X射线衍射(XRD)仪、透射电子显微镜(TEM)、差示... 详细信息

采用浸渍-还原法制备了负载型Co-B/γ-Al2O3非晶态合金催化剂,并将其应用于乳酸乙酯液相加氢制备1,2-丙二醇(1,2-PDO)反应中,研究了其催化加氢性能.采用电感耦合等离子体(ICP)发射光谱仪、X射线衍射(XRD)仪、透射电子显微镜(TEM)、差示扫描量热(DSC)、X射线光电子能谱(XPS)等手段对催化剂的性能进行了表征,考察了制备条件对催化剂性能的影响.结果表明,新鲜的Co-B/γ-Al2O3催化剂具有非晶态结构,Co-B均匀地分散在载体γ-Al2O3上.随着Co负载量的增加,催化剂的热稳定性提高,催化剂表面Co/B原子比增加.当金属Co理论负载量为30%(质量分数,w)时,Co-B/γ-Al2O3催化剂表现出最高的加氢催化性能,在160°C,氢气压力为6.0 MPa条件下反应9 h,乳酸乙酯的转化频率(TOF)为1.41 h-1,转化率达到93.63%,1,2-丙二醇的选择性达到96.10%.催化剂的加氢性能取决于其分散均匀的Co-B纳米粒子、较高的表面Co/B原子比及Co和B之间的电子转移效应.

关键词：非晶态催化剂 Co-B/γ-Al2O3 乳酸乙酯 1,2-丙二醇催化加氢

Mitochondrial Oxidative Stress Enhances Vasoconstriction by Altering Calcium Homeostasis in Cerebrovascular Smooth Muscle Cells under Simulated Microgravity

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2021年第3期34卷 203-212页

作者： LIU Zi Fan WANG hai Ming JIANG Min WANG Lin LIN Le Jian zhao Yun Zhang SHAO Jun Jie ZHOU Jing Jing XIE Man Jiang LI Xin ZHANG Ran Department of Cardiovascular Medicine Chinese PLA General Hospital&Chinese PLA Medical SchoolBeijing 100853China Department of Cardiology The Eighth Medical Center of Chinese PLA General HospitalBeijing 100193China The First Clinical Medical College of Inner Mongolia Medical University Hohhot 010059Inner MongoliaChina Department of Aerospace Physiology Key Laboratory of Aerospace Medicine of Ministry of EducationFourth Military Medical UniversityXi'an 710032ShaanxiChina Division of Health services The First Medical Center of Chinese PLA General HospitalBeijing 100853China

Objective Exposure to microgravity results in postflight cardiovascular deconditioning in *** oxidative stress injury and mitochondrial dysfunction have been reported during this *** elucidate the mechanism for this condition,we investigated whether mitochondrial oxidative stress regulates calcium homeostasis and vasoconstriction in hindlimb unweighted(HU)rat cerebral *** Three-week HU was used to simulate microgravity in *** contractile responses to vasoconstrictors,mitochondrial fission/fusion,Ca^(2+) distribution,inositol 1,4,5-trisphosphate receptor(IP3 R)abundance,and the activities of voltage-gated K+channels(KV)and Ca^(2+)-activated K+channels(BKCa)were examined in rat cerebral vascular smooth muscle cells(VSMCs).Results An increase of cytoplasmic Ca^(2+) and a decrease of mitochondrial/sarcoplasmic reticulum(SR)Ca^(2+) were observed in HU rat cerebral *** abundance of fusion proteins(mitofusin 1/2[MFN1/2])and fission proteins(dynamin-related protein 1[DRP1]and fission-mitochondrial 1[FIS1])was significantly downregulated and upregulated,respectively in HU rat cerebral *** cerebrovascular contractile responses to vasoconstrictors were enhanced in HU rats compared to control rats,and IP3 R protein/mRNA levels were significantly *** current densities and open probabilities of KV and BKCa decreased and increased,*** with the mitochondrial-targeted antioxidant mitoTEMPO attenuated mitochondrial fission by upregulating MFN1/2 and downregulating DRP1/*** also decreased IP3 R expression levels and restored the activities of the KV and BKCa *** restored the Ca^(2+) distribution in VSMCs and attenuated the enhanced vasoconstriction in HU rat cerebral *** The present results suggest that mitochondrial oxidative stress enhances cerebral vasoconstriction by regulating calcium homeostasis during simulated microgravity.

关键词： Microgravity Mitochondrial oxidative stress Calcium homeostasis Vasoconstriction

Cloning and Characterization of Adinbitor,a Novel Disintegrin from the Snake Venom of Agkistrodon halys brevicaudus stejneger

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第6期36卷 425-429页

作者： Ji-Hong WANG, Yu WU, feng REN, Li Lü, and Bao-Chang zhao*Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116027, China Department of Biochemistry and Molecular Biology Dalian Medical UniversityDalian 116027 China

Adinbitor was cloned from Agkistrodon halys brevicaudus stejneger and characterized as a novel disintegrin. In this study, total RNA was extracted from venom gland and used in RT-PCR to generate a cDNA which is 219 bp long. The sequence encoded a polypeptide composed of 73 amino acids, including 12 cysteines, an RGD motif, and the signature motif of disintergrin. Recombinant Adinbitor (rAdinbitor) was expressed in E. coli and purified by using the His?Bind affinity chromatography. The IC50 for inhibiting human platelet aggregation and bFGF-induced proliferation of ECV304 cells was 6 μΜ and 0.89 μΜ respectively. Furthermore, Adinbitor significantly inhibited angiogenesis both in vivo and in vitro. Taken together, these results suggested that Adinbitor had typical functions of disintegrins.

关键词： cloning Adinbitor disintegrin platelet aggregation angiogenesis

Simplified iterative algorithm for OFDM phase noise mitigation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Systems Engineering and Electronics 2007年第4期18卷 716-720页

作者： Yang Jianhua zhao Danfeng Yue Li zhao Chunhui Coll. of Information and Communication Engineering Harbin Engineering Univ. Harbin 150001 P. R. China

OFDM has been widely adopted in several communication systems. However, OFDM systems are very sensitive to the phase noise, which causes the CPE （common phase error） and ICI （inter-carrier interference）, and thus degrades the system performance significantly. Based on the IEEE 802.11a standard, a simplified iterative phase noise mitigation algorithm is proposed. The proposed algorithm has very low complexity, and requires no additional pilot information. The simulation result shows that good BER performance can be achieved through several few times of iteration.

关键词： phase noise OFDM systems IEEE 802.11a standard inter-carrier interference

在线全文

学校读者我要写书评

暂无评论

基于生成式模型的视频基元追踪学习

计算机学报 2010年第10期33卷 1835-1844页

作者：赵友东龚海峰贾云得北京理工大学计算机学院智能信息技术北京市重点实验室北京100081 莲花山研究院湖北鄂州436000

自然场景视频中含有各种类别的视频基元(video primitives),它们构成了整个高维视频块(video bricks)空间,具有不同的结构维度及复杂度,由空间表观与运动共同描述.视频基元主要有两类:结构视频基元与纹理视频基元.文中使用一个通用生成... 详细信息

自然场景视频中含有各种类别的视频基元(video primitives),它们构成了整个高维视频块(video bricks)空间,具有不同的结构维度及复杂度,由空间表观与运动共同描述.视频基元主要有两类:结构视频基元与纹理视频基元.文中使用一个通用生成式模型对两类视频基元进行统一概率建模,每个视频基元的表达能力由其对应的信息增益来度量.利用该度量进行视频基元追踪学习,最终建立一个完整的视频基元集.实验结果显示了文中方法在视频内容表示方面的有效性.

关键词：视频块视频基元生成式模型追踪学习算法视频内容表示

Using transcriptome Shannon entropy to evaluate the off-target effects and safety of insecticidal siRNAs

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第1期21卷 170-177页

作者： MA Wei-hua WU Tong ZHANG Zan LI Hang SITU Gong-ming YIN Chuan-lin YE Xin-hai CHEN Meng-yao zhao Xian-xin HE Kang LI Fei College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070P.R.China State Key Laboratory of Rice Biology/Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests/Institute of Insect Sciences Zhejiang UniversityHangzhou 310058P.R.China College of Plant Protection Nanjing Agricultural UniversityNanjing 210095P.R.China

A recent breakthrough in agricultural biotechnology is the introduction of RNAi-mediated strategies in pest ***, the off-target effects of RNAi pest control are still not fully understood. Here, we studied the off-target effects of two insecticidal siRNAs in both target and non-target insects. The results revealed that off-target effects of insecticidal siRNAs occur widely in both target and non-target insects. We classified the expression-changed genes according to their homology to the siRNA-targeted gene, related KEGG pathways with the siRNA-targeted gene and continuous matches with siRNAs. Surprisingly, the unintended significant changes in gene expression levels did not strictly match with the number of contiguous nucleotides in the siRNAs. As expected, the expression of small portions of the homologous and KEGG-related genes were significantly changed. We calculated the Shannon entropy of the transcriptome profile of the insects after injecting them with insecticidal siRNAs. Though hundreds of genes were affected in their expression levels post siRNAtreatment, the Shannon entropy of the transcriptome remained unchanged, suggesting that the transcriptome expression was balanced. Our results provide evidence that siRNAs cross-reacted with individual genes in non-target species, but did not have significant effects on the integrity of the transcriptome profiles in either target or non-target species on a genomic scale. The metric we proposed can be used to estimate the off-target effects of insecticidal siRNAs, which might be useful for evaluating the safety of RNAi in pest control.

关键词： RNAi off-target effect transcriptome entropy non-target organisms RNAi-mediated pest management

Changes in intracellular and extracellular proteins after ERGIC3 knockdown in lung cancer:Proteins interacting with ERGIC3,HORN,and FLNA

在线全文

学校读者我要写书评

暂无评论

BIOCELL 2023年第8期47卷 1821-1833页

作者： WEI Zhai XIANG ZHENG MENGYUAN LIU QIURONG zhao YUSHU ZHANG YUQING LUO SHANSHAN feng JINJING WANG XUEYING LI MINGSONG WU Special Key Laboratory of Oral Disease Research and High Education Institute in Guizhou Province School of StomatologyZunyi Medical UniversityZunyi563006China Department of Genetics Zunyi Medical UniversityZunyi563006China Department of Pathology Affiliated Hospital of Zunyi Medical UniversityZunyi563008China

Objective:Endoplasmic reticulum-Golgi intermediate compartment 3(ERGIC3)promotes cell proliferation and metastasis in lung cancer,but its molecular mechanism is ***:The GLC-82 cells were randomly divided into the ERGIC3i group and the negative control *** cells were transfected with ERGIC3 siRNA or control siRNA in the groups,*** ERGIC3-interacting proteins expressed in cells or extracellularly were isolated by the immunoprecipitation method and detected by isobaric tags for relative and absolute quantitation and liquid chromatography-tandem mass *** differentially expressed proteins were determined by bioinformatic ***:After ERGIC3 knockdown,88 extracellular differentially expressed proteins,41 upregulated and 47 down-regulated,were detected in the supernatant of cultured *** 52 intracellular differentially expressed proteins,33 were up-regulated and 19 *** analysis showed that the extracellular differential proteins are mainly involved in Ca2+binding and transport and I-kappa B kinase/NF-kappa B signal *** upregulated proteins are mainly involved in the biological process of H3-K27 and H3-K4 methylation in ***-immunoprecipitation assay showed that proteins interacting with ERGIC3 were rich in cytoskeleton construction and RHO GTPases activated p21-activated *** intersection of these two research methods shows that ERGIC3 interacts with HORN and filamin A(FLNA).Conclusion:Proteomic analyses reveal that ERGIC3 acts as a vesicle transmembrane protein on the distribution of various extracellular and intracellular proteins and regulates the extracellular and intracellular biological processes by specifically binding hornin(HORN)and FLNA *** findings maybe provide new methods and ideas for ERGIC3 as a therapeutic target for lung cancer.

关键词： ERGIC3 Lung cancer Proteomics Mass spectrum iTRAQ Bioinformatics

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

串空间理论扩展

软件学报 2005年第10期16卷 1784-1789页

作者：沈海峰薛锐黄河燕陈肇雄中国科学技术大学计算机科学技术系安徽合肥230026 信息安全国家重点实验室(中国科学院软件研究所) 北京100080

现有的串空间模型由于没有抽象更多的密码学原语,因此不能分析较复杂的安全协议.希望通过对串空间理论的扩展使其充分地表达较多的密码学原语,以满足分析复杂安全协议的需要.对入侵串轨迹增加了签名、签名验证和HMAC(keyed-hashingforme... 详细信息

现有的串空间模型由于没有抽象更多的密码学原语,因此不能分析较复杂的安全协议.希望通过对串空间理论的扩展使其充分地表达较多的密码学原语,以满足分析复杂安全协议的需要.对入侵串轨迹增加了签名、签名验证和HMAC(keyed-hashingformessageauthenticationcode)函数模型,重新定义了理想概念并对衍生出的相关命题和定理进行了证明.扩展的诚实理想分析模型不仅继承了原理论的性质,而且适合分析含丰富密码原语的协议,如JFK和IKE2.

关键词：安全协议串空间理想诚实理想