检索结果-南通市图书馆

HSV-1 H129-Derived Anterograde Neural Circuit Tracers:Improvements, Production, and Applications

Neuroscience Bulletin 2021年第5期37卷 701-719页

作者： Hong Yang Feng Xiong Yi-Ge Song hai-Fei Jiang hai-bin qin Jing Zhou Sha Lu Steven FGrieco Xiangmin Xu Wen-Bo Zeng Fei Zhao Min-Hua Luo State Key Laboratory of Virology CAS Center for Excellence in Brain Science and Intelligence TechnologyCenter for Biosafety Mega-ScienceWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Shanghai Genechem Co.Ltd.Shanghai 201203 China Department of Anatomy and Neurobiology School of MedicineUniversity of CaliforniaIrvineCA 92697USA School of Basic Medical Sciences Capital Medical UniversityBeijing 100069China Chinese Institute for Brain Research Beijing 102206China Shanghai Public Health Clinical Center Fudan UniversityShanghai 200032China

Anterograde viral tracers are powerful and essential tools for dissecting the output targets of a brain region of interest. They have been developed from herpes simplex virus 1(HSV-1) strain H129(H129), and have been successfully applied to map diverse neural ***, the anterograde polysynaptic tracer H129-G4 was used by many groups. We then developed the first monosynaptic tracer, H129-dTK-tdT, which was highly successful, yet improvements are needed. Now, by inserting another tdTomato expression cassette into the H129-dTK-tdT genome, we have created H129-dTK-T2, an updated version of H129-dTK-tdT that has improved labeling intensity. To help scientists produce and apply our H129-derived viral tracers, here we provide the protocol describing our detailed and standardized procedures. Commonly-encountered technical problems and their solutions are also discussed in detail. Broadly, the dissemination of this protocol will greatly support scientists to apply these viral tracers on a large scale.

关键词： HSV-1 strain H129(H129) Anterograde transneuronal tracer Polysynaptic tracer Monosynaptic tracer Production Application Neural circuit Neural circuit tracing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis and Conductivity of a One-dimensional Polymer of a Copper(Ⅱ)-porphyrazine with Sulfur Bridges

引用

Chinese Chemical Letters 1998年第7期9卷 639-642页

作者： Zheng He PENG Shou Xing WANG Yi hai WEI and Zi bin qin (Department of Chemistry Wuhan University, Wuhan 430072)

A novel conjugated polymer which shows sendconductivity at T 286K and metallic conductivity at 260K < T< 286K was syllthesized by reaction of 2, 3, 5, 6-tetracyano1, 4-dithiin and phthalonitrile with CuCl2.2H2O.

关键词： Copper(Ⅱ)-porphyrazine one-dimensional polymer semiconductivity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recent progress in constructing fluorinated solid-electrolyte interphases for stable lithium metal anodes

引用

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 270-291页

作者： Di Zhang Pengfei Lv Wei qin Xin He Yuanhua He Civil Aircraft Fire Science and Safety Engineering Key Laboratory of Sichuan Province College of Civil Aviation Safety EngineeringCivil Aviation Flight University of ChinaGuanghan 618307China School of Chemical Engineering Sichuan UniversityChengdu 610065China

Lithium metal batteries(LMBs)are emerging as a promising energy storage solution owing to their high energy density and specific ***,the non-uniform plating of lithium and the potential rupture of the solid-electrolyte interphase(SEI)during extended cycling use may result in dendrite growth,which can penetrate the separator and pose significant short-circuit *** a stable SEI is essential for the long-term operation of the ***-rich SEI has garnered significant attention for its ability to effectively passivate electrodes,regulate lithium deposition,and inhibit electrolyte *** the structural components and preparation methods of existing fluorinated SEI is crucial for optimizing lithium metal anode *** paper reviews the research on optimizing LiF passivation interfaces to protect lithium metal *** focuses on four types of compositions in fluorinated SEI that work synergistically to enhance SEI *** instance,combining compounds with LiF can further enhance the mechanical strength and ionic conductivity of the *** metals with LiF significantly improves electrochemical performance at the SEI/anode interface,with a necessary focus on reducing electron tunneling ***,incorporating polymers with LiF offers balanced improvements in interfacial toughness and ionic conductivity,though maintaining structural stability over long cycles remains a critical area for future *** alloys combined with LiF increase surface energy and lithium affinity,challenges such as dendrite growth and volume expansion *** summary,this paper emphasizes the crucial role of interfacial structures in LMBs and offers comprehensive guidance for future design and development efforts in battery technology.

关键词： LiF lithium metal anodes solid-electrolyte interphase interface cycling stability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

高龄食管癌的手术治疗

引用

苏州大学学报（医学版） 2005年第6期25卷 1070-1071页

作者：马海涛倪斌秦涌何靖康苏州大学附属第一医院心胸外科江苏苏州215006

目的探讨高龄食管癌的外科治疗方法。方法回顾性分析62例高龄(≥75岁)食管癌患者的临床资料。结果高龄食管癌患者术前合并症发生率高(93.5%),术后并发症发生率30.6%,围手术期死亡率1.6%。结论术前评估患者心功能,并结合原发病变的部位... 详细信息

目的探讨高龄食管癌的外科治疗方法。方法回顾性分析62例高龄(≥75岁)食管癌患者的临床资料。结果高龄食管癌患者术前合并症发生率高(93.5%),术后并发症发生率30.6%,围手术期死亡率1.6%。结论术前评估患者心功能,并结合原发病变的部位和范围、手术的时间及难易程度选择手术;加强围手术期管理是决定手术成败的关键。

关键词：高龄患者食管癌外科手术并发症围手术期心功能

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Persistent alterations in gray matter in COVID-19 patients experiencing sleep disturbances:a 3-month longitudinal study

引用

Neural Regeneration Research 2025年第10期20卷 3013-3024页

作者： Kaixuan Zhou Gaoxiong Duan Ying Liu Bei Peng Xiaoyan Zhou Lixia qin Lingyan Liang Yichen Wei qingping Zhang Xiaocheng Li haixia qin Yinqi Lai Yian Lu Yan Zhang Jiazhu Huang Jinli Huang Yinfei Ouyang Bolin bin Mingming Zhao Jun Liu Jianrong Yang Demao Deng Guangxi Key Laboratory of Special Biomedicine School of MedicineGuangxi UniversityNanningGuangxi Zhuang Autonomous RegionChina Department of Radiology the People’s Hospital of Guangxi Zhuang Autonomous RegionGuangxi Academy of Medical SciencesNanningGuangxi Zhuang Autonomous RegionChina Department of Sleep Medicine the People’s Hospital of Guangxi Zhuang Autonomous RegionNanningGuangxi Zhuang Autonomous RegionChina Department of Radiology The Second Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Guangxi Clinical Research Center for Sleep Medicine the People’s Hospital of Guangxi Zhuang Autonomous RegionNanningGuangxi Zhuang Autonomous RegionChina

Sleep disturbances are among the most prevalent neuropsychiatric symptoms in individuals who have recovered from severe acute respiratory syndrome coronavirus 2 *** studies have demonstrated abnormal brain structures in patients with sleep disturbances who have recovered from coronavirus disease 2019(COVID-19).However,neuroimaging studies on sleep disturbances caused by COVID-19 are scarce,and existing studies have primarily focused on the long-term effects of the virus,with minimal acute phase *** a result,little is known about the pathophysiology of sleep disturbances in the acute phase of *** address this issue,we designed a longitudinal study to investigate whether alterations in brain structure occur during the acute phase of infection,and verified the results using 3-month follow-up data.A total of 26 COVID-19 patients with sleep disturbances(aged 51.5±13.57 years,8 women and 18 men),27 COVID-19 patients without sleep disturbances(aged 47.33±15.98 years,9 women and 18 men),and 31 age-and gender-matched healthy controls(aged 49.19±17.51 years,9 women and 22 men)were included in this *** COVID-19 patients with sleep disturbances were included in a longitudinal *** found that COVID-19 patients with sleep disturbances exhibited brain structural changes in almost all brain *** cortical thicknesses of the left pars opercularis and left precuneus were significantly negatively correlated with Pittsburgh Sleep Quality Index ***,we observed changes in the volume of the hippocampus and its subfield regions in COVID-19 patients compared with the healthy *** 3-month follow-up data revealed indices of altered cerebral structure(cortical thickness,cortical grey matter volume,and cortical surface area)in the frontal-parietal cortex compared with the baseline in COVID-19 patients with sleep *** findings indicate that the sleep disturbances patients had altered morphology in the cortical an

关键词： brain structure cortical gray matter volume cortical surface area cortical thickness hippocampus magnetic resonance imaging Pittsburgh Sleep Quality Index severe acute respiratory syndrome coronavirus 2 infections sleep disturbances sub-cortical volume

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

引用

Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi Qi Xueying Chen Bowen Liu Xuliang Lin Xueqing Qiu Yanlin qin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The emerging role of nitric oxide in the synaptic dysfunction of vascular dementia

引用

Neural Regeneration Research 2025年第2期20卷 402-415页

作者： Xiaorong Zhang Zhiying Chen Yinyi Xiong qin Zhou Ling-Qiang Zhu Dan Liu Department of Pathology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Jiujiang Clinical Precision Medicine Research Center JiujiangJiangxi ProvinceChina Center for Cognitive Science and Transdisciplinary Studies Jiujiang UniversityJiangxi ProvinceChina Department of Neurology Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Rehabilitation Affiliated Hospital of Jiujiang UniversityJiujiangJiangxi ProvinceChina Department of Pathophysiology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

With an increase in global aging,the number of people affected by cerebrovascular diseases is also increasing,and the incidence of vascular dementia-closely related to cerebrovascular risk-is increasing at an epidemic ***,few therapeutic options exist that can markedly improve the cognitive impairment and prognosis of vascular dementia *** in Alzheimer’s disease and other neurological disorders,synaptic dysfunction is recognized as the main reason for cognitive *** oxide is one of the ubiquitous gaseous cellular messengers involved in multiple physiological and pathological processes of the central nervous ***,nitric oxide has been implicated in regulating synaptic plasticity and plays an important role in the pathogenesis of vascular *** review introduces in detail the emerging role of nitric oxide in physiological and pathological states of vascular dementia and summarizes the diverse effects of nitric oxide on different aspects of synaptic dysfunction,neuroinflammation,oxidative stress,and blood-brain barrier dysfunction that underlie the progress of vascular ***,we propose that targeting the nitric oxide-sGC-cGMP pathway using certain specific approaches may provide a novel therapeutic strategy for vascular dementia.

关键词： endoplasmic reticulum stress endothelial nitric oxide synthase gene therapy nitric oxide NO-sGC-cGMP pathway synaptic dysfunction vascular dementia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Effects of Several Regulatory Genes on Antibiotic Productivity of the Industrial and its Derived Strains of Streptomyces avermitilis

The Effects of Several Regulatory Genes on Antibiotic Produc...

引用

第二届中国青年学者微生物遗传学学术研讨会

作者： Xia hai-yang, Zhang Liang,Chen Wei-hua, Hu Min-jie, Shen Mei-juan, Huang Jun,Wang hai-bin and qin Zhong-jun Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 20032College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070 R&D Center, Hisun Pharmaceutical Co., Ltd, Taizhou 610091

In the model Streptomyces strains such as Streptomyces ceolicolor and Streptomyces lividans, the antibiotics productivity can be greatly increased by many antibiotic biosynthetic regulatory genes. Here we reported that several known regulatory genes were cloned into an integrative vector and introduced by conjugal transfer from E. coli to the Streptomyces avermitilis industrial stains that produced avermectins or doramectin. The avermectin B1 a yield in strain MMR630 increased

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Construction of an Ordered Cosmid Library of *** for Genetic Modification of the Industrial Strains

Construction of an Ordered Cosmid Library of *** for Genetic...

引用

第二届中国青年学者微生物遗传学学术研讨会

作者： Xia hai-yang, Hu Min-jie, Shen Mei-juan, Zhang Liang, Xie Peng-fei, Huang Jun,Wang hai-bin and qin Zhong-jun Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 20032, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, R&D Center, Hisun Pharmaceutical Co. Ltd, Taizhou 610091

A cosmid library of Streptomyces avermitilis genome was constructed by using vector Supercos-1. Sequencing of the insertion ends of -1000 cosmids showed that they covered 91% genomic sequences of the strain and produced an ordered cosmid library. A genetic manipulation system based on this genomic library was established through the λ-Red mediated recombination and

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Enhancing m^(6)A modification in the motor cortex facilitates corticospinal tract remodeling after spinal cord injury

引用

Neural Regeneration Research 2025年第6期20卷 1749-1763页

作者： Tian qin Yuxin Jin Yiming qin Feifei Yuan Hongbin Lu Jianzhong Hu Yong Cao Chengjun Li Department of Spine Surgery and Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan ProvinceChangshaHunan ProvinceChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Sports Medicine Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

Spinal cord injury typically causes corticospinal tract disruption. Although the disrupted corticospinal tract can self-regenerate to a certain degree, the underlying mechanism of this process is still unclear. N6-methyladenosine(m^(6)A) modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes. However, whether m^(6)A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown. We found that expression of methyltransferase 14 protein(METTL14) in the locomotor cortex was high after spinal cord injury and accompanied by elevated m^(6)A levels. Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury. Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction, we found that METTL14 regulated Trib2 expression in an m^(6)A-regulated manner, thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration. Finally, we administered syringin, a stabilizer of METTL14, using molecular docking. Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14. Findings from this study reveal that m^(6)A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.

关键词： corticospinal tract remodeling epigenetic regulations locomotor cortex m^(6)A modification methyltransferase 14 protein(METTL14) mitogen-activated protein kinase neural regeneration spinal cord injury syringin TRIB2

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：