Construction of an Ordered Cosmid Library of *** for Genetic Modification of the Industrial Strains

作     者：Xia Hai-yang, Hu Min-jie, Shen Mei-juan, Zhang Liang, Xie Peng-fei, Huang Jun,Wang Hai-bin and Qin Zhong-jun Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 20032, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, R&D Center, Hisun Pharmaceutical Co. Ltd, Taizhou 610091 

会议名称：《第二届中国青年学者微生物遗传学学术研讨会》

会议日期：2006年

学科分类：081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

摘      要：A cosmid library of Streptomyces avermitilis genome was constructed by using vector Supercos-1. Sequencing of the insertion ends of -1000 cosmids showed that they covered 91% genomic sequences of the strain and produced an ordered cosmid library. A genetic manipulation system based on this genomic library was established through the λ-Red mediated recombination and