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黄瓜-酸黄瓜异附加系鉴定、果实长度变异及其分子机制

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南京农业大学学报 2025年第1期48卷 108-120页

作者：刘紫薇冯贤波赵勤政夏磊娄群峰南京农业大学园艺学院/作物遗传与种质创新利用全国重点实验室江苏南京210095

[目的]本文旨在通过分析不同黄瓜-酸黄瓜异附加系的果实差异,探究附加系中导入酸黄瓜6号染色体引起黄瓜果实变短的分子机制,为黄瓜近缘野生种优异性状挖掘和利用提供理论依据。[方法]采用分子标记和Oligo-FISh鉴定黄瓜-酸黄瓜异附加系... 详细信息

[目的]本文旨在通过分析不同黄瓜-酸黄瓜异附加系的果实差异,探究附加系中导入酸黄瓜6号染色体引起黄瓜果实变短的分子机制,为黄瓜近缘野生种优异性状挖掘和利用提供理论依据。[方法]采用分子标记和Oligo-FISh鉴定黄瓜-酸黄瓜异附加系中的外源染色体,对栽培黄瓜‘北京截头'(CC3)和不同黄瓜-酸黄瓜异附加系的果实性状进行统计,开展石蜡切片和转录组测序分析,并利用实时荧光定量PCR技术(RT-qPCR)验证转录组测序结果。[结果]共鉴定出7种异附加系材料:附加酸黄瓜6号染色体CC-h06、8号染色体CC-h08、9号染色体CC-h09及10号染色体CC-h10的单体附加系;附加酸黄瓜6和10号染色体CC-h06+h10的双单体附加系;附加酸黄瓜6、7和11号染色体CC-h06+h07+h11以及附加酸黄瓜6、7、10、11和12号染色体CC-h06+h07+h10+h11+h12的多单体附加系。表型统计发现,CC-h08、CC-h09和CC-h10的子房和果实长度均与CC3相似,而CC-h06较CC3明显变短,表明酸黄瓜6号染色体导入会引起CC3果实变短。石蜡切片结果显示,CC-h06的果实细胞相对于CC3较大,但细胞数目却明显减少。转录组分析表明,CC-h06中分别有695和2 770个酸黄瓜及CC3来源的差异表达基因,主要富集在植物激素信号转导、玉米素生物合成、二萜生物合成、色氨酸代谢等激素合成通路。RT-qPCR 验证分析与转录组测定结果一致,说明结果真实可靠。[结论]酸黄瓜6号染色体导入会引起CC3果实变短和果实细胞数量减少,CC-h06的果实变短与生长素、细胞分裂素和赤霉素等植物激素共同调控有关。

关键词：黄瓜酸黄瓜异附加系(ALL) 果实转录组

Low-Cycle Fatigue Properties of Nickel-Based Superalloys Processed by high-Gradient Directional Solidification

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Acta Metallurgica Sinica(English Letters) 2017年第9期30卷 878-886页

作者： Z.D.Fan D.Wang C.Liu G.Zhang J.Shen L.h.lou J.Zhang Superalloys Division Institute of Metal ResearchChineseAcademy of SciencesShenyang 110016China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China Xi'an Thermal Power Research Institute Co. Ltd.Xi'an 710054China

The low-cycle fatigue （LCF） properties of DD10 （single-crystal） and DZ53 （columnar-grained） superalloys solidified by liquid-metal cooling （LMC） and high-rate solidification （hRS） processes have been systematically investi- gated. It was found that the LCF life of DZ53 solidified by LMC was obviously better than that solidified by hRS. In contrast, for DD10, LMC showed no remarkable influences on LCF properties at high temperature and only improved LCF properties at intermediate temperature. Microstructure examination showed that the cracks generally initiated at microp- ores in the subsurface at intermediate temperature. however, the cracks occurred on the surface due to oxidation, or persistent slip bands near script-MC at high temperature. Therefore, the benefits of LMC technique can be attributed to both of the reduced casting defects which significantly affect the LCF properties at intermediate temperature and the improved microstructural homogeneity which was strongly correlated to the LCF properties of alloys at high temperature.

关键词： Superalloys Rapid solidification Low-cycle fatigue Microstructure

Effect of Long-term Thermal Exposure on Microstructure and Stress Rupture Properties of Gh3535 Superalloy

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Journal of Materials Science & Technology 2015年第3期31卷 269-279页

作者： T.Liu J.S.Dong L.Wang Z.J.Li X.T.Zhou L.h.lou J.Zhang School of Materials Science and Engineering Dalian University of Technology Institute of Metal Research Chinese Academy of Sciences Shanghai Institute of Applied Physics Chinese Academy of Sciences Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences

The evolution of microstructure and the stress rupture properties of long term thermally exposed Gh3535 alloy have been investigated. It was found that M6 C carbides presented in the solid solution heat treated samples. During long term thermal exposure at 700 C, fine M12 C carbides precipitated preferentially at grain boundaries. These carbides coexisted with the pre-exiting M6 C. The stress rupture life of700 C/1000 h exposed sample under creep testing at 650 C/324 MPa is 93 h. It is much longer than that of the solid solution samples. No noticeable changes could be detected in both the microstructure and stress rupture lives when the samples were exposed for time longer than 1000 h M12 C carbides were found to be beneficial to the creep properties. The cracks initiated at the interface of M6 C carbides and matrix, which led to a lower creep rupture life.

关键词： Gh3535 superalloy Long term thermal exposure Micro

Co-Poisson structures on polynomial hopf algebras

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Science China Mathematics 2018年第5期61卷 813-830页

作者： Qi lou Quanshui Wu School of Mathematical Sciences Fudan University

The hopf dual h~? of any Poisson hopf algebra h is proved to be a co-Poisson hopf algebra provided h is noetherian. Without noetherian assumption, unlike it is claimed in literature, the statement does not hold. It is proved that there is no nontrivial Poisson hopf structure on the universal enveloping algebra of a non-abelian Lie algebra. So the polynomial hopf algebra, viewed as the universal enveloping algebra of a finite-dimensional abelian Lie algebra, is considered. The Poisson hopf structures on polynomial hopf algebras are exactly linear Poisson structures. The co-Poisson structures on polynomial hopf algebras are *** correspondences between co-Poisson and Poisson structures are also established.

关键词： Poisson algebra co-Poisson coalgebra Poisson hopf algebra co-Poisson hopf algebra

MdDGK3-like as a negative regulator participates in ALA-induced PP2AC to promote stomatal opening in apple leaves

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horticultural Plant Journal 2023年第5期9卷 898-908页

作者： Zheng Chen Yingrui lou Liangju Wang College of horticulture Nanjing Agricultural UniversityNanjing 210095China

5-Aminolevulinic acid(ALA)can inhibit abscisic acid(ABA)-induced stomatal ***,the molecular mechanism is *** this study,we found that ALA upregulated the MdPP2AC expression and PP2A activity in the apple(Malus domestica ***.‘Fuji’)*** the promoter of MdPP2AC as bait,a diacylglycerol kinase MdDGK3-like was selected by the Yeast One hybrid(Y1h)from the cDNA library of the epidermis of apple leaves treated by exogenous *** to binding the promoter of MdPP2AC,MdDGK3-like was found to inhibit the transcription activity of MdPP2AC promoter,while ALA significantly eliminated the role of *** tobacco leaves,MdDGK3-like was localized in the nucleus of stomatal guard ***,MdDGK3-like might act as a transcription factor negatively regulating MdPP2AC expression and causing stomatal *** further identify MdDGK3-like functions,several transiently transgenic apple leaves(including overexpression and interference)were *** results revealed that overexpression of MdDGK3-like promoted stomatal closure by increasing Ca^(2+)and h_(2)O_(2)and decreasing flavonol levels in the guard ***,MdDGK3-like(i)led the stomatal opening with lower levels of Ca^(2+)and h_(2)O_(2)but higher *** on these,we proposed a new hypothesis that ALA up-regulated MdPP2AC expression via negatively regulating the expression of MdDGK3-like to up-regulate PP2A expression and the enzyme activity,which improved the stomatal *** it was the first time that MdDGK3-like was showed to act as a transcription factor,the proposed model provided a new insight onto the mechanisms of ALA-induced stomatal opening.

关键词： ALA Apple MdDGK3-like MdPP2AC Stomatal opening Transcription factor

Multimodal separation for ultrasonic Lamb wave using independent component analysis in the time-frequency domain

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Chinese Journal of Acoustics 2021年第4期40卷 583-596页

作者： lou Sijia LIU Zhenli XU Kailiang TRAN Tho N.h.T. ZhOU Chang LE Lawrence h. TA De'an Center for Biomedical Engineering Fudan UniversityShanghai 200433 Academy for Engineering and Technology Fudan UniversityShanghai 200433 Ningbo Marvoto Intelligent Technology Ningbo 315300 Department of Radiology and Diagnostic Imaging University of Alberta EdmontonCanada T6G2R3

Aiming at facilitating Lamb wave signal analysis and mode characterization,an ultrasonic-Lamb wave niultimodal separation method combining short-time Fourier transform(STFT)and independent component analysis(ICA)was *** signals were projected into the time-frequency domain through *** that the energy trajectory of each Lamb mode is indepenclent in the time-frequency domain,ICA method was then applied to separate tlie superposed *** group velocity curve of each mode was extracted according to its time-frequency energy trajectory,based on which the plate thickness can be further *** finite-difference time-domain(FDTD)simulated signals and experimental signals for a 1 nmi-thick steel plate were used to validate the performance of the proposed *** three fundainental Lamb modes,A0,SO and A1 modes were *** average errors of group velocity estimation in the simulation and experiment were about 1.5%and 2.0%,with that of plate thickness estimation being 0.3%and 2.0%*** simulated results indicate that,when the signal-to-noise ratio(SNR)is larger than 0 dB,the ICA method in the time-frequency domain can be used for ultrasonic Lamb wave multimodal separation,group velocity extraction and plate thickness estimation.

关键词： separation wave analysis

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西藏猪hEV流行病学调查和组织病理学观察

中国兽医杂志 2025年第1期61卷 94-99页

作者：娄永志辛娇娇索朗斯珠贡嘎西藏农牧学院动物科学学院西藏林芝860000

为掌握西藏猪戊型肝炎病毒(hEV)流行情况,本试验采集西藏藏猪血清样本313份,西藏林芝约克猪肝脏样本53份;对藏猪血清进行hEV-IgG抗体检测,并进行t检验分析;对约克猪肝脏样本进行反转录聚合酶链式反应(RT-PCR),检测hEV开放阅读框2(ORF2)... 详细信息

为掌握西藏猪戊型肝炎病毒(hEV)流行情况,本试验采集西藏藏猪血清样本313份,西藏林芝约克猪肝脏样本53份;对藏猪血清进行hEV-IgG抗体检测,并进行t检验分析;对约克猪肝脏样本进行反转录聚合酶链式反应(RT-PCR),检测hEV开放阅读框2(ORF2)基因保守区域,对RT-PCR阳性产物测序后进行系统发育进化和同源性分析,确定西藏猪hEV基因型;通过实时荧光定量PCR(qPCR)和苏木精-伊红(h.E.)染色对阳性肝脏组织进行病原定量和病理分析。结果显示,313份西藏藏猪血清样本中hEV-IgG抗体总阳性率为35.46%(111/313),其中,公藏猪和母藏猪抗体阳性率分别为39.31%(68/173)和30.71%(43/140);1~2月龄仔猪、2~4月龄保育猪、4~6月龄育肥猪和8月龄以上猪抗体阳性率分别为27.87%(17/61)、32.93%(27/82)、43.00%(43/100)和34.29%(24/70)。西藏约克猪肝脏样本中检测到2份hEV RNA阳性样本,分别命名为TLZyork1和TLZyork2,GenBank登录号为PP464289和PP464290,拷贝数为3.15×10^(3)和2.81×10^(4)copies/mL,与hEV 4a、4b、4c、4d基因型同源性为73.9%~97.4%;系统发育进化树分析显示,约克猪TLZyork1和TLZyork2均属于hEV 4d基因型。约克猪hEV RNA阳性肝脏样本h.E.染色结果显示,肝脏组织中大量肝细胞水肿,中央静脉周围肝细胞呈气球样变性,少量淋巴细胞浸润等。综上,西藏藏猪和约克猪中存在hEV流行,提示相关部门应进一步重视对西藏猪hEV的防控。

关键词：藏猪约克猪戊型肝炎病毒(hEV) 流行病学调查组织病理学染色

甲烷在ZnO表面反应制合成气的微观机理研究

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原子与分子物理学报 2025年第6期42卷 1-9页

作者：黄琼谊楼波丁佳欣王水安华南理工大学电力学院广州510640

利用密度泛函(DFT)和过渡态理论对甲烷分子在ZnO表面的微观反应过程进行第一性原理研究,通过建立ZnO表面的吸附模型,计算了吸附能、过渡态参数和态密度,探讨了甲烷分子在氧化锌晶体表面h解离以及h_(2)和CO的生成过程,结果表明:Ch_(4)在Z... 详细信息

利用密度泛函(DFT)和过渡态理论对甲烷分子在ZnO表面的微观反应过程进行第一性原理研究,通过建立ZnO表面的吸附模型,计算了吸附能、过渡态参数和态密度,探讨了甲烷分子在氧化锌晶体表面h解离以及h_(2)和CO的生成过程,结果表明:Ch_(4)在ZnO(010)表面的最佳吸附位点为Zn位,吸附能为-4.65 kcal/mol,甲烷在氧化锌ZnO(010)面四次解离h(反应动力学上最有利路径)所需克服的能垒分别为38.6 kcal/mol、90.8 kcal/mol、53.2 kcal/mol和35.8 kcal/mol,其中甲烷第二次解离h_(2)过程能垒最高,是反应过程的决速步,降低该基元反应的活化能是关键,而-Oh的形成抑制了-Ch_(3)的解离.氧空位的形成增加活性位点的数量与提高位点的活性.甲烷与氧化锌表面的反应有两次h_(2)生成反应,第一次生成h_(2)的反应难度较大.

关键词：化学链重整甲烷反应机理密度泛函理论过渡态

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Novel efficient procedure for biodiesel synthesis from waste oils with high acid value using 1-sulfobutyl-3-methylimidazolium hydrosulfate ionic liquid as the catalyst

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Chinese Journal of Chemical Engineering 2017年第10期25卷 1519-1523页

作者： Shuanglan hu Ying Li Wenyong lou School of Biosciences and Bioengineering South China University of Technology Guangzhou 510640 China School of Food Sciences and Engineering South China University of Technology Guangzhou 510640 China State Key Laboratory of Pulp and Paper Engineering South China University of Technology Guangzhou 510840 China

Preparation of biodiesel from waste oils containing 72% of free fatty acids catalyzed by a novel Br?nsted acidic ionic liquid 1-sulfobutyl-3-methylimidazolium hydrosulfate([BhSO_3MIM][hSO_4]) was systematically *** optimum molar ratio of methanol to waste oils,catalyst amount,reaction temperature and reaction time were 8/1,10%(based on the mass of waste oils),140°C and 6 h,respectively,under which the obtained yield of biodiesel reached 94.9%.Also,[BhSO_3MIM][hSO_4] as a catalyst still retained around 97% of its original catalytic activity after successive re-use of 5 batches(6 h per batch),showing the excellent operational ***,the acidic IL [BhSO_3MIM][hSO_4] was able to ef ficiently catalyze conversions of waste oils with different amounts of FFAs(free fatty acids) into biodiesel,and showed tremendous application ***,an ef ficient and environmentally friendly catalyst is provided for the synthesis of biodiesel from waste oils with high acid value.

关键词： 1-Sulfobutyl-3-methylimidazolium hydrosulfate Biodiesel Waste oils with high acid value Catalyst