MdDGK3-like as a negative regulator participates in ALA-induced PP2AC to promote stomatal opening in apple leaves

作     者：Zheng Chen Yingrui Lou Liangju Wang Zheng Chen;Yingrui Lou;Liangju Wang

作者机构：College of HorticultureNanjing Agricultural UniversityNanjing 210095China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2023年第9卷第5期

页      面：898-908页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.32172512) the Jiangsu Special Fund for Frontier Foundation Research of Carbon Peaking and Carbon Neutralization(Grant No.BK20220005) Jiangsu Agricultural Science and Technology Innovation Fund[Grant No.CX(20)2023] a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions 

主　　题：ALA Apple MdDGK3-like MdPP2AC Stomatal opening Transcription factor 

摘      要：5-Aminolevulinic acid(ALA)can inhibit abscisic acid(ABA)-induced stomatal ***,the molecular mechanism is *** this study,we found that ALA upregulated the MdPP2AC expression and PP2A activity in the apple(Malus domestica ***.‘Fuji’)*** the promoter of MdPP2AC as bait,a diacylglycerol kinase MdDGK3-like was selected by the Yeast One Hybrid(Y1H)from the cDNA library of the epidermis of apple leaves treated by exogenous *** to binding the promoter of MdPP2AC,MdDGK3-like was found to inhibit the transcription activity of MdPP2AC promoter,while ALA significantly eliminated the role of *** tobacco leaves,MdDGK3-like was localized in the nucleus of stomatal guard ***,MdDGK3-like might act as a transcription factor negatively regulating MdPP2AC expression and causing stomatal *** further identify MdDGK3-like functions,several transiently transgenic apple leaves(including overexpression and interference)were *** results revealed that overexpression of MdDGK3-like promoted stomatal closure by increasing Ca^(2+)and H_(2)O_(2)and decreasing flavonol levels in the guard ***,MdDGK3-like(i)led the stomatal opening with lower levels of Ca^(2+)and H_(2)O_(2)but higher *** on these,we proposed a new hypothesis that ALA up-regulated MdPP2AC expression via negatively regulating the expression of MdDGK3-like to up-regulate PP2A expression and the enzyme activity,which improved the stomatal *** it was the first time that MdDGK3-like was showed to act as a transcription factor,the proposed model provided a new insight onto the mechanisms of ALA-induced stomatal opening.