检索结果-南通市图书馆

Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice

在线全文

学校读者我要写书评

暂无评论

National Science Review 2020年第1期7卷 92-101页

作者： Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo he Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-zhu Chen Xin-Xin guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen Department of Neurology and Institute of Neurology The First Affiliated Hospital of Fujian Medical University Fujian Key Laboratory of Molecular Neurology Fujian Medical University Institute of Neuroscience State Key Laboratory of Neuroscience Key Laboratory of Primate Neurobiology CAS Center for Excellence in Brain Science and Intelligence Technology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Key Lab of Computational Biology CAS-MPG Partner Institute for Computational Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Department of Anatomy Histology & Embryology Shanghai Medical College Fudan University Cold Spring Harbor Laboratory Cold Spring Harbor

We here report a genome-editing strategy to correct spinal muscular atrophy(SMA). Rather than directly targeting the pathogenic exonic mutations, our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements. We disrupted intronic splicing silencers(ISSs, including ISS-N1 and ISS + 100) of survival motor neuron(SMN) 2, a key modifier gene of SMA, to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs. Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration. Furthermore, co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9) or Cas9 from Staphylococcus aureus(SaCas9) alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56% and 100% of severe SMA transgenic mice(Smn-/-, SMN2tg/-).The median survival of the resulting mice was extended to >400 days. Collectively, our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases.

关键词： spinal muscular atrophy SMN2 splicing-regulatory elements CRISPR/Cas9 germline correction

The osmolyte-producing endophyte Streptomyces albidoflavus OsiLf-2 induces drought and salt tolerance in rice via a multi-level mechanism

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2022年第2期10卷 375-386页

作者： Shuqi Niu Yan Gao Huixian Zi Ying Liu Xuanming Liu Xianqiu Xiong Qingqing Yao Ziwei Qin Ning Chen Liang guo Yuanzhu Yang Peng Qin Jianzhong Lin Yonghua zhu Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation College of BiologyHunan UniversityChangsha 410008HunanChina Yuan Longping High-Tech Agriculture Co. Ltd.Changsha 410001HunanChina

Drought and salinity are major environmental stresses that impair crop growth and productivity worldwide. Improving drought and salt tolerance of crops with microbial mutualists is an effective and environmentally sound strategy to meet the demands of the ever-growing world population. In the present study, we found that the Streptomyces albidoflavus Osi Lf-2, a moderately salt-tolerant endophytic actinomycete, produced abundant osmolytes, including proline, polysaccharides, and ectoine. Inoculation with Osi Lf-2 increased the osmotic-adjustment ability of the rice host by increasing the proline content(by250.3% and 49.4%) and soluble sugar(by 20.9% and 49.4%) in rice under drought and salt conditions, relative to the uninoculated control. Osi Lf-2 increased stress responses in the rice host at the physiological and biochemical levels(photosynthesis efficiency, osmolytes and antioxidant content), and the gene level(osmolytes synthesis, stress-responsive and ion-transport related genes), raising rice yields under both greenhouse and saline–alkaline soil conditions. The use of endophytic actinomycetes offers a promising biotechnological approach to developing stress-tolerant plants.

关键词： Rice Endophytic actinomycete Osmolytes Salt tolerance Drought tolerance

在线全文

学校读者我要写书评

暂无评论

职业教育课程思政的价值理性与教育逻辑

民族教育研究 2021年第5期32卷 44-54页

作者：郭和才朱德全西南大学教育学部岭南师范学院

随着现代社会的非线性发展和智能技术的复杂化演变,党和国家对技术技能人才的诉求将由工具性需求转至价值性需求,这要求职业教育在人才培养过程中不仅要助推技术技能人才在"器物"层面的完善,更要注重其在"价值"层面的塑造。课程思政作... 详细信息

随着现代社会的非线性发展和智能技术的复杂化演变,党和国家对技术技能人才的诉求将由工具性需求转至价值性需求,这要求职业教育在人才培养过程中不仅要助推技术技能人才在"器物"层面的完善,更要注重其在"价值"层面的塑造。课程思政作为新时代党和国家实现"立德树人"根本目标和"三全育人"的创新之举,将助推职业教育构建新的育人模式。然而,现实中职业教育课程思政面临工具理性膨胀和价值理性式微的二元矛盾,这种价值危机对职业教育课程思政的价值本源、价值向度、价值维度及价值旨归构成威胁。未来,亟须通过构建职业教育课程思政的教育"社会性"逻辑、教育"人本性"逻辑、教育"历史性"逻辑与教育"实践性"逻辑,发挥职业教育课程思政价值塑造的特殊功能,进而助推职业教育实现"立德树人"和"三全育人"的根本任务。

关键词：立德树人三全育人职业教育课程思政价值理性

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

爆震波多管点火特性实验

航空动力学报 2005年第5期20卷 895-899页

作者：郭红杰梁国柱马彬北京航空航天大学宇航学院北京100083

为了研究液体火箭发动机爆震波多管点火的同步性能、多次点火重复性能以及点火火炬性能,组建了氢氧爆震波多管点火实验系统.采用氢气和氧气为工质,常温供气压力0.1～0.5 MPa(表压),混合比1.8～7.2,进行了多次实验.实验结果表明:爆震波... 详细信息

为了研究液体火箭发动机爆震波多管点火的同步性能、多次点火重复性能以及点火火炬性能,组建了氢氧爆震波多管点火实验系统.采用氢气和氧气为工质,常温供气压力0.1～0.5 MPa(表压),混合比1.8～7.2,进行了多次实验.实验结果表明:爆震波点火技术可以在与液体火箭发动机贮箱压力相适应的较低的供气压力下获得高温(＞1300℃)高压(＞1 MPa)爆震产物,并且具备良好的点火重复性能和多管点火同步性能,多次点火重复性时间差和多管点火同步性时间差均小于0.3 ms.爆震波多管点火技术适合用于多燃烧室液体火箭发动机的同步点火.

关键词：航空、航天推进系统液体推进剂火箭发动机爆震波点火技术实验研究

3种不同方法在宫颈癌筛查分流中的价值对比分析

在线全文

学校读者我要写书评

暂无评论

诊断病理学杂志 2024年第6期31卷 508-512页

作者：袁明明葛婷婷章小霞陶玉梅姜林甫朱国祥黄国琴任晓燕南通大学附属南通妇幼保健院(南通市妇幼保健院) 江苏南通226018

目的探讨液基细胞学检测(TCT)、DNA定量分析以及人乳头瘤病毒(HPV)检测3种技术方法在宫颈癌筛查中的应用及诊断价值。方法以病理组织学结果为金标准,通过TCT、DNA定量分析以及HPV 3种方法对其诊断准确性、灵敏度、特异度、阳性预测值、... 详细信息

目的探讨液基细胞学检测(TCT)、DNA定量分析以及人乳头瘤病毒(HPV)检测3种技术方法在宫颈癌筛查中的应用及诊断价值。方法以病理组织学结果为金标准,通过TCT、DNA定量分析以及HPV 3种方法对其诊断准确性、灵敏度、特异度、阳性预测值、阴性预测值等进行比较分析。结果300例患者中,病理组织学131例为宫颈炎症,117例为低级别鳞状上皮内病变(LSIL),43例为高级别鳞状上皮内病变(HSIL),9例为鳞状细胞癌(SCC)。TCT的诊断灵敏度92.90%、特异度88.55%、符合率91.00%;DNA的诊断灵敏度95.86%、特异度91.60%、符合率94.00%;HPV的诊断灵敏度97.63%、特异度81.68%、符合率90.67%;TCT+HPV联合的诊断灵敏度98.22%、特异度80.92%、符合率90.67%;DNA+HPV联合的诊断灵敏度99.41%、特异度81.68%、符合率91.67%。工作特征曲线(ROC)分析结果为AUC(HPV)>AUC(DNA)>AUC(DNA+HPV)>AUC(TCT+HPV)>AUC(TCT)。结论TCT+HPV及DNA+HPV两种联合筛查方案降低了漏诊率。运用HPV初筛结合DNA定量分析进行分流的方法准确性会比3种方法单独筛查更高。

关键词：宫颈癌液基细胞学 DNA定量分析 HPV检测分流

The Preparation of Unsymmetrical Diaminodimethylsilanes and the Cleavage of Silicon-Nitrogen Bonds by Acid Chloride

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1997年第11期8卷 945-946页

作者： guo Bin RONG Ru Jian MA Chong Ying ZHOU zhu Liang JIN Wan Nian WU Qing WANG (Department of Chemistry, East China University of Science and Technology, Shanghai, 200237) Department of Chemistry E. China Univ. of Sci./Technology Shanghai 200237 China

Seven unsymmetrical diaminodimethylsilanes were prepared. The reactions of these silylamine with benzoyl chloride indicated that in comparison with electronic, the steric effect played more important role on the reactivity of Si-N bond. As a new method, unsymmetrical diamide can produced by the reaction of the title compounds with diacid chloride.

关键词： Si The Preparation of Unsymmetrical Diaminodimethylsilanes and the Cleavage of Silicon-Nitrogen Bonds by Acid Chloride

Is 1.28 parts per million biomarker specific for neural progenitor cells?

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2010年第15期5卷 1125-1129页

作者： Zhifeng Xu Chongyang Shen Haiqiang zhu Yaowen Chen Linping Wu Peng Huang YeyuXiao Zhiwei Shen Li Pang Xiuqin guo Lian Ma Renhua Wu Department of Medical Imaging Second Affiliated Hospital Medical College of Shantou University Shantou 515041 Guangdong Province China Multidisciplinary Research Center of Shantou University Shantou 515041 Guangdong Province China Department of Paediatrics Second Affiliated Hospital Medical College of Shantou University Shantou 515041 Guangdong Province China

Nuclear magnetic resonance-visible mobile lipid, at 1.28 parts per million （ppm）, is thought to be due to mobile lipid droplets formed in cells and has been considered unique for neural progenitor cells. However, this idea remains controversial. The present study examined the 1.28 ppm biomarker in other stem cells and non-stem cells, and explored the relationship between 1.28 ppm biomarker and mobile lipid droplets. 1H nuclear magnetic resonance spectroscopy of EC109 cells, mesenchymal stem cells （MSCs） and adipogenic cells differentiated from MSCs was performed. Results show that 1.28 ppm biomarker was observed in human MSCs, but was absent from EC109 cells. Following adipogenic differentiation induced for 2 weeks, the 1.28 ppm biomarker climbed remarkably, with mobile lipid droplet generation, suggesting that the 1.28 ppm biomarker is not specific for neural progenitor cells because it is also observed in MSCs and adipogenic-induced differentiated cells. Moreover, it is possible to monitor MSCs differentiation following cell transplantation, using 1.28 ppm biomarker changes.

关键词： magnetic resonance spectroscopy mesenchymal stem cell adipogenic differentiation biomarker lipid droplet

在线全文

学校读者我要写书评

暂无评论

疑似神经行为发育迟缓幼儿相关影响因素研究

安徽医科大学学报 2024年第7期59卷 1244-1250页

作者：李小嫚黄丽娟王筱刘祖民郭润民农雪艳黄秀娟朱锦渊陈艳婷杜进林广东医科大学公共卫生学院流行病与卫生统计学系东莞523000 广东医科大学顺德妇女儿童医院儿童保键科佛山528399 广东医科大学顺德妇女儿童医院科研科佛山528399

目的通过调查顺德区24~60月龄幼儿神经行为发育情况,探讨幼儿发育影响因素,为幼儿神经行为发育异常的干预提供参考。方法采用回顾性队列研究方法,将经小儿神经心理发育量表初筛分数≤85分的幼儿纳入研究。初筛分数≤85分可能存在发育迟... 详细信息

目的通过调查顺德区24~60月龄幼儿神经行为发育情况,探讨幼儿发育影响因素,为幼儿神经行为发育异常的干预提供参考。方法采用回顾性队列研究方法,将经小儿神经心理发育量表初筛分数≤85分的幼儿纳入研究。初筛分数≤85分可能存在发育迟缓的风险,需进一步采用GESELL发育诊断量表进行发育迟缓的诊断,调查幼儿出生时的基本信息和其母亲的基本信息,以及收集幼儿完成GESELL发育诊断量表时的基本信息。结果共纳入幼儿271例,男童196例、女童75例。幼儿5个能区中语言能区发育商最低(P<0.001)。多重线性回归分析结果显示:与女童比较,男童语言能区发育商降低5.321分(P=0.049,95%CI:-10.620~-0.021),个人-社交能区发育商降低4.474分(P=0.023,95%CI:-8.316~-0.631);与自然分娩幼儿比较,剖宫产幼儿大运动能区发育商降低4.890分(P=0.008,95%CI:-8.499~-1.281),精细动作能区发育商降低3.373分(P=0.037,95%CI:-6.532~-0.213),语言能区发育商降低7.621分(P=0.004,95%CI:-12.826~-2.416),个人-社交能区发育商降低6.232分(P=0.001,95%CI:-10.006~-2.457)。二分类Logistic回归分析结果显示:与自然分娩幼儿比较,剖宫产幼儿大运动能区发育迟缓风险增加(OR=1.763,95%CI:1.003~3.100),精细动作能区发育迟缓风险增加(OR=2.217,95%CI:1.235~3.980),语言能区发育迟缓风险增加(OR=3.306,95%CI:1.080~10.124)。结论疑似神经行为发育迟缓的幼儿语言能区发育较其他能区落后,且男童语言及个人-社交能区发育商较女童低,剖宫产幼儿发育较自然分娩幼儿迟缓。应重点关注幼儿语言以及男童语言、个人-社交发育情况,慎重选择分娩方式,重视在早期对幼儿展开全面神经行为发育的评估。

关键词：幼儿分娩方式新生儿促甲状腺激素神经行为发育发育迟缓

维普期刊数据库博看期刊

Design,synthesis and antifungal activity of carbazole derivatives

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2014年第2期25卷 229-233页

作者： Shi-Ping zhu Wen-Ya Wang Kun Fang Zheng-Gang Li guo-Qiang Dong Zhen-Yuan Miao Jian-Zhong Yao Wan-Nian Zhang Chun-Quan Sheng School of Pharmacy Second Military Medical University

The incidence of invasive fungal infections is increasing rapidly. Clinically available antifungal agents suffer from limited efficacy and severe resistance. There is an urgent need to discover antifungal lead compounds with novel chemical scaffold. On the basis of our previously identified tetrahydrocarbazole antifungal leads, the structure-activity relationship was further explored by modifying the scaffold and the side chains. Several targeted compounds showed potent activity against Candida species. Particularly, compound 13i showed better antifungal activity than the lead compound, which can be used as a good starting point for further optimization.

关键词： Carbazol Antifungal activity Structure-activity relationship