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Susceptibility to AcMNPV and Expression of Recombinant Proteins by a Novel Cell Clone Derived from a Trichoplusia ni QAU-BTI-Tn9-4s Cell line

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Virologica Sinica 2011年第5期26卷 297-305页

作者： Ming Shan Shi-ying Zhang Lei Jiang Ming Ma guo-xun li A Center for Advanced Invertebrate Cell Engineering Collaborated Between Boyce Thompson Institute and Qingdao Agricultural University Qingdao Agricultural University （QAU） Qingdao 266109 China Boyce Thompson Institute （BTI） at Cornell University Ithaca New York 14853 USA

It is well known that Tn5B1-4 (commercially known as the High Five) cell line is highly susceptible to baculovirus and provides superior production of recombinant proteins when compared to other insect cell *** the characteristics of the cell line do not always remain stable and may change upon continuous *** an alphanodavirus,named Tn5 Cell line Virus (or TNCL Virus),was identified in High Five cells in ***,we established a new cell line,QB-Tn9-4s,from Trichoplusia ni,which was determined to be free of TNCL virus by RT-PCR *** this paper,we describe the development of a novel cell clone,QB-CL-B,from a low passage QB-Tn9-4s cell line and report its susceptibility to AcMNPV,and the level of recombinant protein *** cell clone was similar to its parental cells QB-Tn9-4s and Tn5B1-4 cells in morphology and growth rate;although it also showed approximately the same responses to AcMNPV infection and production of occlusion bodies,there were higher levels of recombinant protein production in comparison to QB-Tn9-4s (parental cells) and High5 cells.

关键词： Cell line Insect virus Recombinant protein expression RT-PCR TNCL virus

Metal/covalent organic frameworks for aqueous rechargeable zinc-ion batteries

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Science China Chemistry 2024年第1期67卷 247-259页

作者： Hu Hong xun guo Jiaxiong Zhu Zhuoxi Wu Qing li Chunyi Zhi Department of Materials Science and Engineering City University of Hong KongHong Kong 999077China

lithium-ion batteries(liBs)have become one of the most successful energy storage systems due to their high operating voltage,high energy density,and long cycle ***,with the widespread use of liBs in recent decades,lithium resources are at risk of being ***,it is necessary to find a substitute for liBs to meet the needs of future large-scale energy storage *** of their competitiveness,low cost,and high safety,aqueous rechargeable zinc-ion batteries(ARZIBs)are regarded as promising components in the post-lithium-ion-battery *** the tunable composition,ordered porous channels,and controllable structure of metal-organic frameworks(MOFs)and covalent organic frameworks(COFs),these frameworks are viewed as potential materials for developing high-performance *** this review,we focus on the recent developments in the applications of MOF-/COF-based materials in ARZIBs,including in electrode materials,anode modifications,separators,and solid *** then focus on the critical factors and optimization techniques of MOF-/COF-based materials that affect the performance of ***,we conclude with some projections for the expansion of ARZIBs containing MOF-/COF-based materials.

关键词： metal/covalent organic frameworks aqueous zinc-ion batteries electrode materials anode modifications separators electrolytes

Establishment, Growth kinetics, and Susceptibility to AcMNPV of Heat Tolerant Lepidopteran Cell lines

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Virologica Sinica 2011年第3期26卷 198-205页

作者： Yan-lei WU Lei Jiang Yoshifumi Hashimoto Robert R. Granados guo-xun li The Center for Advanced Invertebrate Cell Engineering Collaboration with BTI USA Qingdao AgriculturalUniversity Qingdao 266109 China＇~ Boyce Thompson Institute for Plant Research at Cornell University Ithaca NY 14853 USAJ

Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. Adaptation of cell lines sf-9, BTI-TN-5B1-4 (High5) and BTI-TN-MG1 (MG1) to 33℃ and 35℃ was carried out by shifting the culture temperature between 28℃ and higher temperatures by a gradual stepwise increase in temperature. The process of adaption to a higher culture temperature was accomplished over a period of 2 months. The cell lines with the temperature adaption were designated as sf9-ht33, sf9-ht35, High5-ht33, High5-ht35, MG1-ht33, MG1-ht35. These cell lines have been subcultured over 70 passages. Adaption to high temperatures was confirmed by a constant population doubling time with individual cell lines. The population doubling time of heat adapted cell lines were 1-4 h less than these of parental cell lines. Cell shapes did not show obvious change, however, the cell size of sf9-ht cells was enlarged and those of High5 and MG1 ht cells were reduced after heat adaption. When the cell lines were infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) at 28℃, 33℃, 35℃ and 37℃, production of budded virus and occlusion bodies in each cell line was optimum at its own adapted temperature.

关键词： Heat-tolerant cell lines Susceptibility of virus Production of virus Growth curves Insect cell lines

Cooperative guidance law with maneuverability awareness:A decentralized solution

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Chinese Journal of Aeronautics 2024年第7期37卷 450-457页

作者： Shuyang XU xun SONG Chaoyong li College of Electrical Engineering Zhejiang UniversityHangzhou 310027China Beijing Institute of Electronic System Engineering Beijing 100074China

In this paper,we propose a cooperative guidance law aimed to achieve coordinated impact angles with limited observation on target *** primary challenge lies in establishing an appropriate communication graph among all missiles and devising an algorithm to estimate target acceleration information during *** address this,we propose a specific communication topology and employ a numerical integration-based estimation ***,a distributed algorithm is introduced to facilitate consensus on target acceleration *** upon these foundations,we design an optimal-control-based distributed guidance law for each *** of the proposed guidance law is validated through numerical simulations.

关键词： Cooperative guidance Target maneuver estimation Distributed estimation Consensus Optimal control

A novel evasion guidance for hypersonic morphing vehicle via intelligent maneuver strategy

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Chinese Journal of Aeronautics 2024年第5期37卷 441-461页

作者： xun li Xiaogang WANG Hongyu ZHOU Yu li Department of Aerodynamics Harbin Institute of TechnologyHarbin 150001China Beijing Aerospace Technology Institute Beijing 100074China

This paper presents a novel evasion guidance law for hypersonic morphing vehicles,focusing on determining the optimized wing's unfolded angle to promote maneuverability based on an intelligent ***,the pursuit-evasion problem is modeled as a Markov decision *** the agent's action consists of maneuver overload and the unfolded angle of wings,which is different from the conventional evasion guidance designed for fixed-shape *** reward function is formulated to ensure that the miss distances satisfy the prescribed bounds while minimizing energy ***,to maximize the expected cumulative reward,a residual learning method is proposed based on proximal policy optimization,which integrates the optimal evasion for linear cases as the baseline and trains to optimize the performance for nonlinear engagement with multiple ***,offline training guarantees improvement of the constructed evasion guidance law over conventional ***,the guidance law for online implementation includes only analytical *** maps from the confrontation state to the expected angle of attack and the unfolded angle while retaining high computational *** show that the proposed evasion guidance law can utilize the change of unfolded angle to extend the maximum overload *** it surpasses conventional maneuver strategies by ensuring better evasion efficacy and higher energy efficiency.

关键词： Hypersonic vehicles Variable-sweep wings Evasion guidance Reinforcement learning Pursuit-evasion problem

Perspective on new cell-free DNA technologies for early cancer detection

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Cancer Biology & Medicine 2024年第2期21卷 139-143页

作者： Jie li xun Lan Department of Basic Medical Sciences School of MedicineTsinghua UniversityBeijing 100084China MOE Key Laboratory of Bioinformatics Tsinghua UniversityBeijing 100084China Tsinghua-Peking Joint Center for life Sciences Tsinghua UniversityBeijing 100084China

High cancer heterogeneity and aggressiveness are major factors contributing to the poor prognosis of patients with advanced cancer~(1-5).Early detection of cancer can significantly improve the prognosis of patients with early-stage cancer after standard *** traditional cancer detection technologies,such as endoscopy for gastrointestinal cancer,mammography for breast cancer。

关键词： prognosis cancer patients

Effects of Number of Bleed Holes on Shock-Wave/Boundary-Layer Interactions in a Transonic Compressor Stator

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Journal of Thermal Science 2024年第2期33卷 611-624页

作者： li Bai ZHOU xun LUO Lei DU Wei School of Energy Science and Engineering Harbin Institute of TechnologyHarbin150001China

An extensive numerical investigation is conducted to characterize the flow separation control in a transonic compressor cascade with a porous *** bleed holes are arranged on the suction surface in a single row,two staggered rows and three staggered *** each bleed scheme,five bleed pressure ratios are examined at an inlet Mach number of *** results indicate that the aerodynamic performance of the cascade is significantly improved by the porous *** the single-row scheme,the maximum reduction in total pressure losses is 57%.For the two-staggered-row and three-staggered-row schemes,there is an optimal bleed pressure ratio of 1.0,and the maximum reductions in total pressure loss are 68% and 75%,*** low loss in the cascade is due to the well-controlled boundary *** new local supersonic region created by the bleed hole is the key reason for the improved boundary *** vortex induced by side bleeding provides another mechanism for delaying flow *** the bleed holes could create multiple local supersonic regions,which reduce the range of the adverse pressure gradient that the boundary layer needs to *** is the reason why cascades with more bleed holes perform better.

关键词： transonic compressor stator shock wave/boundary layer interaction porous bleed number of bleed holes

Double-confined nanoheterostructure Sb/Sb_(2)S_(3)@Ti_(3)C_(2)T_(x)@C toward ultra-stable li-/Na-ion batteries

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Rare Metals 2024年第5期43卷 2067-2079页

作者： Dan Wang Qun Ma Huan He Zhi-Yuan Wang Run-guo Zheng Hong-Yu Sun Yan-guo liu Chun-li liu School of Materials Science and Engineering Northeastern UniversityShenyang110819China School of Resources and Materials Northeastern University at QinhuangdaoQinhuangdao 066004China Key Laboratory of Dielectric and Electrolyte Functional Material Hebei Province Qinhuangdao 066004China Department of Physics and Oxide Research Center Hankuk University of Foreign StudiesYongin 17035Korea

Antimony-based materials with high capacities and moderate potentials are promising anodes for lithium-/-sodium-ion ***,their tremendous volume expansion and inferior conductivity lead to poor structural stability and sluggish reaction ***,a doubleconfined nanoheterostructure Sb/Sb_(2)S_(3)@Ti_(3)C_(2)T_(x)@C has been fabricated through a solvothermal method followed by low-temperature heat *** dual protection of“MXene”and“carbon”can better accommodate the volume expansion of Sb/Sb_(2)S_(3).The strong covalent bond(Ti-S,Ti-O-Sb,C-O-Sb)can firmly integrate Sb-based material with Ti_(3)C_(2)T_(x)and carbon,which significantly improves the structure *** addition,the carbon layer can restrain the oxidation of MXenes,and the nano-Sb/Sb_(2)S_(3)can facilitate electron/ion transport and suppress the restacking of *** heterogeneous interface between Sb and Sb_(2)S_(3)can further promote interfacial charge *** MXene-Sb/Sb_(2)S_(3)@C-1 with the optimal Sb content shows high specific capacities,comparable rate properties and ultra-stable cycling performances(250 m Ah·g^(-1)after 2500 cycles at 1 A·g^(-1)for sodium-ion batteries).Ex situ X-ray diffractometer(XRD)test reveals the storage mechanism including the conversion and alloying process of MXene-Sb/Sb_(2)S_(3)@*** voltammetry(CV)test results demonstrate that the pseudocapacitance behavior is dominant in MXene-Sb/Sb_(2)S_(3)@C-1,especially at large *** design paves the way for exploring high-performance alloy-based/conversion-type anode for energy storage devices.

关键词： Sb/Sb_(2)S_(3) MXene Carbon Anode lithium-ion batteries Sodium-ion batteries

Predictive model for non-malignant portal vein thrombosis associated with cirrhosis based on inflammatory biomarkers

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1213-1226页

作者： guo-Le Nie Jun Yan Ying li Hong-Long Zhang Dan-Na Xie Xing-Wang Zhu xun li The First School of Clinical Medicine Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of General Surgery The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina

BACKGROUND Portal vein thrombosis(PVT),a complication of liver cirrhosis,is a major public health *** prediction is the most effective method for PVT diagnosis and *** To develop and validate a nomogram and network calculator based on clinical indicators to predict PVT in patients with *** Patients with cirrhosis hospitalized between January 2016 and December 2021 at the First Hospital of Lanzhou University were screened and 643 patients with cirrhosis who met the eligibility criteria were *** a 1:1 propensity score matching 572 patients with cirrhosis were screened,and relevant clinical data were *** risk factors were identified using the least absolute shrinkage and selection operator(LASSO)and multivariate logistic regression *** inflation factors and correlation matrix plots were used to analyze multicollinearity among the variables.A nomogram was constructed to predict the probability of PVT based on independent risk factors for PVT,and its predictive performance was verified using a receiver operating characteristic curve(ROC),calibration curves,and decision curve analysis(DCA).Finally,a network calculator was constructed based on the *** This study enrolled 286 cirrhosis patients with PVT and 286 without *** analysis revealed 13 variables as strongly associated with PVT *** logistic regression analysis revealed nine indicators as independent PVT risk factors,including etiology,ascites,gastroesophageal varices,platelet count,D-dimer,portal vein diameter,portal vein velocity,aspartate transaminase to neutrophil ratio index,and platelet-to-lymphocyte *** and correlation matrix plot results revealed no significant multicollinearity or correlation among the variables.A nomogram was constructed based on the screened independent risk *** nomogram had excellent predictive performance,with an area under the ROC curve of 0.821 and 0.829 in the

关键词： Portal vein thrombosis liver cirrhosis Nomogram Inflammatory markers Aspartate aminotransferase to neutrophil ratio index Platelet-to-lymphocyte ratio