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Time lag effect of vegetation response to seasonal precipitation in the Mara River Basin

Ecological Processes 2023年第1期12卷 665-679页

作者： Shouming Feng Zhenke Zhang Shuhe Zhao xinya guo Wanyi Zhu Priyanko Das School of Geography and Oceanography Sciences Nanjing UniversityNanjing 210023China Institute of African Studies Nanjing UniversityNanjing 210023China Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application Nanjing 210023China

Background Mara River Basin is an ecologically fragile area in East Africa,with a pattern of alternating wet and dry seasons shaped by periodic *** the regional biological traits and climatic change,the vegetation’s response to seasonal variation is complicated and frequently characterized by time *** study analyzed the variation of the Normalized Difference Vegetation Index(NDVI)and investigated its time lag to precipitation at the monthly *** characteristic peaks were proposed from the perspective of seasonal mechanisms and were quantified to assess the lag *** The results showed that the Anomaly Vegetation Index could identify low precipitation in 2006,2009,and *** NDVI showed an increasing trend in 75%of areas of the basin,while showed a decreased significance in 3.5%of areas,mainly in *** to the time lag,the 1-month lag effect dominated most months,and the spatiotemporal disparities were *** method considering the alternations of wet and dry seasons found that the time lag was approximately 30 *** on the time distribution of NDVI characteristic peaks,the average time lag was 35.5 days and increased with the range of *** The findings confirmed an increasing trend of NDVI in most regions from 2001 to 2020,while the trends were most obvious in the downstream related to human *** results could reflect the time lag of NDVI response to precipitation,and the 1-month lag effect dominated in most months with spatial *** NDVI characteristic peaks were found to be efficient indicators to assess the seasonal characteristics and had a great potential to quantify vegetation variation.

关键词： Mara River Basin NDVI Seasonal precipitation Characteristic peak Time lag

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Comparison of gene expression responses of zebrafish larvae to Vibrio parahaemolyticus infection by static immersion and caudal vein microinjection

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Aquaculture and Fisheries 2021年第3期6卷 267-276页

作者： xinya guo Ce Ji Xuan Du Jianfeng Ren Yao Zu Weiming Li Qinghua Zhang Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China National Pathogen Collection Center for Aquatic Animals Ministry of AgricultureShanghai Ocean UniversityShanghai201306China Department of Fisheries and Wildlife Michigan State UniversityEast LansingMI48824USA

infection model for studying inflammation and the innate immune *** investigated the genes and signaling pathways activated by static immersion and caudal vein microinjection infection using transcriptome profiling and reverse-transcription quantitative PCR(RT-qPCR)to compare the innate immune response in 3 days post-fertilization(dpf)zebrafish larvae infected by Vibrio parahaemolyticus Vp13 *** median lethal dose(LD50)values at 96 h following immersion and microinjection were 3.63×107 CFU/mL and 5.76×102 CFU/nL,*** innate immune response was initiated after 2 h of incubation with the respective LD50 for each infection *** hundred and two genes in the immersion group and 359 genes in the microinjection group were activated and differentially expressed ***-three Gene Ontology(GO)terms and four Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways were significantly enriched in the immersion group,compared with only three GO terms and no KEGG pathways in the microinjection *** genes,tnfb and ccl20a.3,were significantly up-regulated in both *** speculated that immersion infection may affect initial dorsal determination,cytochromes,and fatty acid-binding proteins,as well as inflammation,while microinjection infection may mainly directly affect the immune *** with doses>LD50(1.09×109 CFU/mL and 1.09×103 CFU/nL by immersion and microinjection,respectively)caused more significant up-regulation of il11a,tnfa,tnfb,il1b,ccl34a.4,ccl20a.3,irak3,cxcl18b,and ccl35.1,suggesting that in addition to the classical innate immunity genes tnfa,tnfb,il1b,and il6,the genes il11a,ccl34a.4,ccl20a.3,cxcl18b,and ccl35.1 were also important for defending against Vp13 *** findings highlight the genes involved in the responses of zebrafish to Vp13 infection via different routes and doses,and thus provide the basis for further analyses of immune response signaling pathways.

关键词： Immersion Microinjection Transcriptome Vibrio parahaemolyticus Zebrafish

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ALS抑制剂类除草剂抗性水稻品系迁粳187选育及抗性鉴定

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大麦与谷类科学 2024年第3期41卷 25-32页

作者：郭新亚陈春李凡王飞飞史玉良陈卫军江苏省农业科学院宿迁农科所江苏泗阳223700

乙酰乳酸合酶[ALS,也称为乙酰羟酸合酶(AHAS),EC 4.***.3.18]抑制剂类除草剂是目前治理水稻田内杂草的高效化学防除方法,以金粳818、迁粳187这2个抗性水稻品种(系)为材料,徐稻3号为敏感型对照,分析经咪唑乙烟酸处理后靶标基因的相对表达... 详细信息

乙酰乳酸合酶[ALS,也称为乙酰羟酸合酶(AHAS),EC 4.***.3.18]抑制剂类除草剂是目前治理水稻田内杂草的高效化学防除方法,以金粳818、迁粳187这2个抗性水稻品种(系)为材料,徐稻3号为敏感型对照,分析经咪唑乙烟酸处理后靶标基因的相对表达量。研究发现,抗性材料的靶标基因相对表达量逐步下降,且最终下降至正常水平的60%左右。离体酶活检测结果表明,金粳818、迁粳187这类ALS发生Ser-653-Asn氨基酸替换的材料对咪唑乙烟酸的抗性是敏感型材料徐稻3号的千倍以上。

关键词： ALS 除草剂金粳818 迁粳187

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The Study of the Damage Laws of Pool Fires

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Journal of Thermal Science 1995年第4期4卷 271-275页

作者： Yu Deming Feng Changgen Zeng Qingxuan guo xinya (Mechanics & Engineering Department, Beijing Institute of Technology, Beijing 100081, China) Mechanics & Engineering Department Beijing Institute of Technology Beijing China

This paper studies pool fires in open air and their damage laws. First, it discusses the damage criteria of thermal radiation, then presents an algorithm to predict damage radii of pool fires, and performs numerical calculations by using a software called POOLFIRE, which has been developed by the authors, finally discusses the possible ways to improve the algorithm and gives the conclusions obtained from the study.

关键词： pool fires damage criteria damage radii algorithm numerical calculation.

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Notch1a play an important role in innate immune response of zebrafish larvae challenged by Vibrio parahaemolyticus

Notch1a play an important role in innate immune response of ...

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第十三届全国免疫学学术大会

作者： Ce Ji xinya guo Weiming Li Qinghua Zhang Shanghai Ocean University Michigan State University

Background: Notch signaling pathway has been reported to regulate innate immunity by interacting with TLR signaling pathway. However, there were absence of understanding of comprehensive role of Notch molecule in innate immune ***: The aim of this study is to explore the innate immune function of Notch1 a ***: Using Vibrio parahaemolyticus strain Vp13 to challenge 3 dpf WT and notch1 azebrafish larvae, and calculating the LD value. In addition, Illumina Hiseq 4000 was used to generate transcriptome data which were comprehensively analyzed. Quantitative real-time RT-PCR were adopted to validate the differentially expressed genes(DEGs) identified by transcriptome and analyze DEGs expression pattern of different infection time ***: There are 359 significantly DEGs, including 246 significantly down-regulated genes and 113 significantly up-regulated genes in WT infection groups compared with WT control groups. And 986 significantly DEGs were found in notch1 ainfection groups compared with notch1 acontrol groups, in which 82 genes were significantly down-regulated and 904 genes were significantly up-regulated. These DEGs belonged to tumor necrosis factor(TNF) family, complement family, nuclear factor kappa B(NFκB) family, cathepsin family, interleukin(IL) family, chemokine family, serpin peptidase inhibitor family, matrix metallopeptidase family, innate immune cells related, pattern recognition receptor(PRR) related, and other cytokines. The DEGs of il1 b, tnfb, nfkbiaa, ctslb, il13 ra1 and c3 a.3 were subjected to quantitative gene expression analysis, and the results were consistent with those of the transcriptome ***: Our results indicating that Notch1 a play important roles to inhibit innate immune related genes, and could comprehensively mediate innate immune response by regulating TLR, NLR, lectin, complement, IL, chemokine, TNF, cathepsin and serpin. Further studies were also required for understanding the part

关键词： Innate immune Notch1a Transcriptome Zebrafish larvae

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大豆油体蛋白基因GmOLE2的功能分析

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南京农业大学学报 2021年第3期44卷 477-486页

作者：郭新亚章文华林峰南京农业大学生命科学学院江苏南京210095

[目的]本文旨在对编码栽培大豆(Glycine max)油体蛋白(oleosin,OLE)的GmOLE2基因进行功能研究,分析其蛋白结构、在油脂合成中的调控作用以及对非生物胁迫的响应。[方法]运用生物信息学方法,分析其蛋白保守结构、亲疏水性以及进化关系;... 详细信息

[目的]本文旨在对编码栽培大豆(Glycine max)油体蛋白(oleosin,OLE)的GmOLE2基因进行功能研究,分析其蛋白结构、在油脂合成中的调控作用以及对非生物胁迫的响应。[方法]运用生物信息学方法,分析其蛋白保守结构、亲疏水性以及进化关系;利用农杆菌侵染获得转基因拟南芥纯合株系,并利用气相色谱检测转基因拟南芥种子脂肪酸组分;对转基因拟南芥进行多种非生物胁迫处理,观察并统计相关性状和指标。[结果]GmOLE2蛋白具有OLE蛋白家族高度保守的结构域——脯氨酸结,且蛋白中间部分是一个由约24个氨基酸组成的超长疏水性结构域。GmOLE2是种子特异性表达的基因,过表达GmOLE2导致拟南芥种子内总蛋白含量增加约25%,总可溶糖含量下降10%,油脂含量下降约10%,饱和脂肪酸和三不饱和脂肪酸含量减少,单不饱和脂肪酸含量增加。过表达GmOLE2拟南芥种子可以缓解冻害,-20℃处理后的种子萌发率仍达到75%,是野生型萌发率的2倍。GmOLE2 mRNA积累受MeJA和ABA调控:10μmol·L^(-1) MeJA处理GmOLE2过表达拟南芥幼苗,其主根生长受到抑制的情况下,侧根丰度及长度显著大于未处理组,且在24 h内GmOLE2基因表达量显著增加,是未处理组的8~10倍。将GmOLE2过表达拟南芥的种子播种在含0.5μmol·L^(-1) ABA的培养基上,萌发3 d后,经RT-qPCR检测发现GmOLE2基因表达量显著升高,是未处理组的8倍。[结论]GmOLE2基因具有典型油体蛋白结构特征。在拟南芥中过表达GmOLE2基因,虽然减少种子总油含量但可以调节脂肪酸构成,且其种子具有较好的抗冻性,可受MeJA和ABA诱导表达参与植物对非生物胁迫的响应。

关键词：油体蛋白脂肪酸非生物胁迫大豆

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Primary function of zebrafish Notch3 in innate immunity

Primary function of zebrafish Notch3 in innate immunity

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第十二届全国免疫学学术大会

作者： ZHANG QINGHUA YUE QIANWEN JI CE guo xinya ZHANG XIAOMING DING AN ZU YAO REN JIANFENG LI WEIMING Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationCollege of Fisheries and Life ScienceShanghai Ocean University Department of Fisheries and Wildlife Michigan State University

Zebrafish(Danio rerio) has many advantages in the field of innate immune ***3 is one of the four Notch receptors identified in *** researches had shown that notch3 is an important receptor in Notch signaling pathway and play an essential role during the development in zebrafish,but the function of Notch3 in innate immune response is not well *** elucidate the function of Notch3 in innate immune response,we used CRISPR/Cas9 system to knock out notch3 gene and observed the phenotype and tested LD at 96 h using Vibrio parahaemolyticus(Vp) and Aeromonas hydrophila(Ah)to challenge the 4 days post fertilization(dpf) zebrafish larvae by immersion and caudal vein *** results showed that the brain hemorrhage and trunk curvature in zebrafish mutant larvae which is consistent with the previous *** data showed that LD were 2.37×10 CFU/mL of and 1.75×10 CFU/mL in wild type(WT) and notch3 mutants by immersion,whereas,they were 5.76×10 CFU/mL and 4.70×10 CFU/mL by caudal vein injection,which suggested that the notch3 mutants were weaker than WT in the resistance to Vp and more easier to die during *** the contrary,WT were more susceptible to Ah infection than in notch3 mutants according to the LD at 96 h were 6.65×10 CFU/mL of WT and 4.86×10 CFU/mL of notch3 mutants by immersion,3.57×10 CFU/mL of WT and9.77×10 CFU/mL of notch3 mutants by caudal vein *** conclude,we found notch3 maybe take part in an important role in innate immunity,but the mechanism in the immune response to different pathogenic bacteria leading to the different results need to be studied in detail in the future.

关键词： zebrafish notch3 CRISPR/Cas9 bacteria innate immunity

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汾河河津段主要污染物时间序列分析

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中国环境监测 2016年第1期32卷 70-74页

作者：郭新亚张兴奇南京大学地理与海洋科学学院江苏南京210023

基于Mann-Kendall趋势检验法和小波分析法,研究了汾河河津段2004—2013年高锰酸盐指数、氨氮、溶解氧含量时间序列的变化趋势和突变特性。结果表明:高锰酸盐指数和氨氮含量有显著下降趋势,溶解氧含量有显著上升趋势。高锰酸盐指数和氨... 详细信息

基于Mann-Kendall趋势检验法和小波分析法,研究了汾河河津段2004—2013年高锰酸盐指数、氨氮、溶解氧含量时间序列的变化趋势和突变特性。结果表明:高锰酸盐指数和氨氮含量有显著下降趋势,溶解氧含量有显著上升趋势。高锰酸盐指数和氨氮含量在1、2月较大,8、9月较小;溶解氧含量在1、2月较大,6、7月较小。高锰酸盐指数含量在2004—2009年差异较大;氨氮含量在2004—2013年差异均较大;溶解氧含量在2010—2013年差异较大。

关键词：高锰酸盐指数氨氮溶解氧小波分析

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我国草地保险保障机制研究与实践——来自美国的经验启示

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金融理论与实践 2022年第1期 99-107页

作者：郭新雅赵元凤内蒙古农业大学经济管理学院内蒙古呼和浩特010018

2020年8月我国首单政策性草地保险试点启动,为了进一步完善我国草地保险制度,在梳理我国政策性草地保险实施方案特征的基础上,发现我国草地保险存在亟待解决的问题:整个区域费率统一、保障水平单一、保障功能不足、保险责任与保障目标... 详细信息

2020年8月我国首单政策性草地保险试点启动,为了进一步完善我国草地保险制度,在梳理我国政策性草地保险实施方案特征的基础上,发现我国草地保险存在亟待解决的问题:整个区域费率统一、保障水平单一、保障功能不足、保险责任与保障目标不符、理赔机制不完善。基于以上问题,以全球草地保险机制较为完善的美国草地指数保险为例,分析其运行机制,总结其保险合约设计的核心内容。研究表明:第一,指数保险是美国草地保险的主导产品类型;第二,美国草地指数保险顶层设计是以"联邦政府支持下的商业化运作模式"运营,由国家海洋和大气管理局气候预测中心、地球资源观测卫星和风险管理局等组织保障;第三,合约设计是利用网格进行风险区划和费率厘定,提供多档次可供选择的保障水平,以月度为单位设计保险期间,设置了差异化的保费补贴政策。最后,提出美国草地保险制度对我国草地保险可持续发展的启示。

关键词：保险研究草地指数保险运行机制国际经验借鉴

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基于Budyko假设和SCS-CN模型的河源区水源涵养功能研究

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水土保持研究 2018年第1期25卷 147-152页

作者：杜世勋郭新亚荣月静山西省生态环境研究中心太原030000

河源区作为一种重要生态功能区,具有重要的生态系统服务功能。以沁河源区为例,利用Budyko假设和SCS-CN模型研究了其水源涵养功能空间分布特征,并比较了不同土地利用类型、海拔和坡度下的水源涵养能力。结果表明:(1)沁河源区多年平均水... 详细信息

河源区作为一种重要生态功能区,具有重要的生态系统服务功能。以沁河源区为例,利用Budyko假设和SCS-CN模型研究了其水源涵养功能空间分布特征,并比较了不同土地利用类型、海拔和坡度下的水源涵养能力。结果表明:(1)沁河源区多年平均水源涵养量为306.48mm,水源涵养总量为4.19亿m^3;(2)不同土地利用类型水源涵养功能不同,落叶阔叶林和常绿针叶林的水源涵养贡献率和水源涵养量均值最大,贡献率分别为43.99%和42.00%,水源涵养量均值分别为330.41mm和364.53mm;(3)不同海拔高度上水源涵养功能不同,海拔1 000~1 500m地区是水源涵养功能的主体,水源涵养量均值在海拔1 500~2 000m地区最大,为401.71mm;(4)不同坡度上水源涵养功能不同,缓坡和斜坡的水源涵养贡献率最大,急坡、陡坡和险坡上水源涵养量均值分别为373.62mm,368.32mm和363.94mm。

关键词：河源区水源涵养 budyko假设 SCS-CN模型

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