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Assembly-free reads accurate identification(AFRAID)approach outperforms other methods of DNA barcoding in the walnut family(Juglandaceae)

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Plant Diversity 2025年第1期47卷 115-126页

作者： Yanlei liu Kai Chen Lihu Wang Xinqiang Yu Chao Xu Zhili Suo shiliang Zhou Shuo shi Wenpan Dong School of Landscape and Ecological Engineering Hebei University of EngineeringHandan 056038China State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Life Science Hebei Normal UniversityShijiazhuang 050024China School of Ecology and Nature Conservation Beijing Forestry UniversityBeijing 100083China

DNA barcoding has been extensively used for species ***,species identification of mixed samples or degraded DNA is limited by current DNA barcoding *** this study,we use plant species in Juglandaceae to evaluate an assembly-free reads accurate identification(AFRAID)method of species identification,a novel approach for precise species identification in ***,we determined(1)the accuracy of DNA barcoding approaches in delimiting species in Juglandaceae,(2)the minimum size of chloroplast dataset for species discrimination,and(3)minimum amount of next generation sequencing(NGS)data required for species *** found that species identification rates were highest when whole chloroplast genomes were used,followed by taxon-specific DNA barcodes,and then universal DNA *** identification of 100%was achieved when chloroplast genome sequence coverage reached 20%and the original sequencing data reached 500,000 *** accurately identified species for all samples tested after 500,000 clean reads,with far less computing time than common *** results provide a new approach to accurately identify species,overcoming limitations of traditional DNA *** method,which uses next generation sequencing to generate partial chloroplast genomes,reveals that DNA barcode regions are not necessarily fixed,accelerating the process of species identification.

关键词： DNA barcode Species identification Random DNA barcode Juglandaceae Assembly-free

Shenmai Injection Reduces Cardiomyocyte Apoptosis Induced by Doxorubicin through miR-30a/Bcl-2

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CHINESE JOURNAL OF INTEGRATIVE MEDICINE 2025年第3期31卷 240-250页

作者： Zhang, Xiao-nan Li, Yan-yang Lyu, shi-chao Jia, Qiu-jin Zhang, Jun-ping liu, Long-tao China Acad Chinese Med Sci Xiyuan Hosp Natl Clin Res Ctr Chinese Med Cardiol Dept Cardiovasc Med Beijing 100091 Peoples R China Tianjin Med Univ Dept Integrated Tradit Chinese & Western Med Canc Inst & Hosp Tianjin 300060 Peoples R China Tianjin Univ Tradit Chinese Med Dept Cardiovasc Med Teaching Hosp 1 Tianjin 300193 Peoples R China Tianjin Key Lab Tradit Res Tradit Chinese Med Pres Dept Cardiovasc Med Tianjin 300193 Peoples R China

Objective: To explore the molecular mechanism of Shenmai Injection (SMI) against doxorubicin (DOX) induced cardiomyocyte apoptosis. Methods: A total of 40 specific pathogen-free (SPF) male Sprague Dawley (SD) male rats were divided into 5 groups based on the random number table, including the control group, the model group, miR-30a agomir group, SMI low-dose (SMI-L) group, and SMI high-dose (SMI-H) group, with 8 rats in each group. Except for the control group, the rats were injected weekly with DOX (2 mg/kg) in the tail vein for 4 weeks to induce myocardial injury, and were given different regimens of continuous intervention for 2 weeks. Cardiac function was detected by echocardiography and myocardial pathological changes were observed by Van Gieson (VG) staining. Myocardial injury serum markers, including creatine kinase (CK), lactate dehydrogenase (LDH), troponin T (cTnT), N-terminal pro-brain natriuretic peptide (NT-proBNP), soluble ST2 (sST2), and growth differentiation factor-15 (GDF-15) were detected by enzyme linked immunosorbent assay (ELISA). Cardiomyocyte apoptosis was observed by terminal deoxynucleotidyl transferase-mediated biotinylated dUTP triphosphate nick end labeling (TUNEL) and transmission electron microscopy, and the expressions of target proteins and mRNA were detected by Western blot and quantitative real time polymerase chain reaction (qRT-RCR), respectively. Results: The treatment with different doses of SMI reduced rat heart mass index and left ventricular mass index (P<0.05), significantly improved the left ventricular ejection fraction (P<0.05), decreased the levels of serum CK, LDH, cTnT, and NT-proBNP (P<0.05 or P<0.01), reduced the levels of serum sST2 and GDF-15 (P<0.05 or P<0.01), decreased the collagen volume fraction, reduced the expressions of rat myocardial type I and type III collagen (P<0.05 or P<0.01), and effectively alleviated myocardial fibrosis. And the study found that SMI promoted the expression levels of miR-30a and Bc

关键词： Shenmai Injection doxorubicin cardiotoxicity miR-30a/Bcl-2 apoptosis

Contribution of China's carbon budget on global radiative forcing

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Journal of Geographical Sciences 2025年第2期35卷 233-251页

作者： LI Nan CUI Yaoping liu Xiaoyan shi Zhifang LI Mengdi Michael E MEADOWS Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions (Henan University) Ministry of Education College of Environment and Planning Henan University Dabieshan National Observation and Research Field Station of Forest Ecosystem at Henan Department of Environmental and Geographic Sciences University of Cape Town

China is the world's largest carbon dioxide（CO2） emitter and a major trading country. Both anthropogenic and natural factors play a critical role in its carbon budget. However,previous studies mostly focus on evaluating anthropogenic emissions or the natural carbon cycle separately, and few included trade-related（import and export） CO2emissions and its contribution on global warming. Using the CarbonTracker CT2019 assimilation dataset and China trade emissions from the Global Carbon Project, we found that the change trend of global CO2flux had obvious spatial heterogeneity, which is mainly affected by anthropogenic CO2flux. From 2000 to 2018, carbon emissions from fossil fuels in the world and in China all showed an obvious increasing trend, but the magnitude of the increase tended to slow *** 2018, the radiative forcing（RF） caused by China's import and export trade was-0.0038 W m-2, and the RF caused by natural carbon budget was-0.0027 W m-2, offsetting 1.54% and 1.13% of the RF caused by fossil fuels that year, respectively. From 2000 to 2018, the contribution of China's carbon emission from fossil fuels to global RF was 11.32%. Considering China's import and export trade, the contribution of anthropogenic CO2emission to global RF decreased to 9.50%. Furthermore, taking into account the offset of carbon sink from China's terrestrial ecosystems, the net contribution of China to global RF decreased to 7.63%. This study demonstrates that China's terrestrial ecosystem and import and export trade are all mitigating China's impact on global anthropogenic warming, and also confirms that during the research process on climate change, comprehensively considering the carbon budget from anthropogenic and natural carbon budgets is necessary to systematically understand the impacts of regional or national carbon budgets on global warming.

关键词： China anthropogenic carbon emissions natural carbon sinks carbon dioxide radiative forcing contribution

Arsenic methylation and microbial communities in paddy soils under alternating anoxic and oxic conditions

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Journal of Environmental Sciences 2025年第2期148卷 468-475页

作者： Jing liu Rui Pei Runzeng liu Chuanyong Jing Wenjing liu Shandong Key Laboratory of Environmental Processes and Health School of Environmental Science and EngineeringShandong UniversityQingdao 266237China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China

Arsenic(As)methylation in soils affects the environmental behavior of As,excessive accumulation of dimethylarsenate(DMA)in rice plants leads to straighthead disease and a serious drop in crop *** the mobility and transformation of methylated arsenic in redox-changing paddy fields is crucial for food ***,soils including unarsenic contaminated(N-As),low-arsenic(L-As),medium-arsenic(M-As),and high-arsenic(H-As)soils were incubated under continuous anoxic,continuous oxic,and consecutive anoxic/oxic treatments respectively,to profile arsenic methylating process and microbial species involved in the As *** anoxic-oxic(A-O)treatment,methylated arsenic was significantly increased once oxygen was introduced into the incubation *** methylated arsenic concentrations were up to 2-24 times higher than those in anoxic(A),oxic(O),and oxic-anoxic(O-A)treatments,under which arsenic was methylated slightly and then decreased in all four As concentration *** fact,the most plentiful arsenite S-adenosylmethionine methyltransferase genes(arsM)contributed to the increase in As ***(40.8%-62.4%),Firmicutes(3.5%-15.7%),and Desulfobacterota(5.3%-13.3%)were the major microorganisms related to this *** microbial increasedmarkedly and played more important roles after oxygen was introduced,indicating that they were potential keystone microbial groups for As methylation in the alternating anoxic(flooding)and oxic(drainage)*** novel findings provided newinsights into the reoxidation-driven arsenic methylation processes and the model could be used for further risk estimation in periodically flooded paddy fields.

关键词： Arsenic methylation Anoxic-oxic Straighthead disease arsM gene Microbial community

The circadian clock affects starvation resistance through the pentose phosphate pathway in silkworm,Bombyx mori

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Insect Science 2025年第1期32卷 55-68页

作者： Tai-Ming Dai Jian-Feng Qiu Cheng Luo Wen-Zhao Cui Kai liu Jiang-Lan Li Ruji Peng Yang-Hu Sima shi-Qing Xu School of Life Sciences Suzhou Medical College of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Agricultural Biotechnology&Ecology(IABE) Soochow UniversitySuzhouJiangsu ProvinceChina

Disruption of the circadian clock can affect starvation resistance,but the molecular mechanism is still ***,we found that starvation resistance was significantly reduced in the core gene BmPer deficient mutant silkworms(Per−/−),but the mutant's starvation resistance increased with larval *** natural physiological conditions,the weight of mutant 5th instar larvae was significantly increased compared to wild type,and the accumulation ability of triglycerides and glycogen in the fat bodies was ***,under starvation conditions,the weight consumption of mutant larvae was increased and cholesterol utilization was *** analysis showed that beta-oxidation was significantly upregulated under starvation conditions,fatty acid synthesis was inhibited,and the expression levels of genes related to mitochondrial function were significantly *** investigations revealed that the redox balance,which is closely related to mitochondrial metabolism,was altered in the fat bodies,the antioxidant level was increased,and the pentose phosphate pathway,the source of reducing power in cells,was *** findings suggest that one of the reasons for the increased energy burden observed in mutants is the need to maintain a more robust redox balance in metabolic *** necessitates the diversion of more glucose into the pentose phosphate pathway to ensure an adequate supply of reducing power.

关键词： Bombyx mori circadian clock oxidative stress pentose phosphate pathway starvationresistance

Metastasizing to the colon from triple-negative breast cancer:A case report and review of literature

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World Journal of Gastrointestinal Oncology 2025年第3期17卷 443-455页

作者： Ben-Quan Wang Xing-gui Fang Ben-Hong Xiang Xiao-Jiao Wang Ming Cao Cheng-Le Zhuang Zhong-Chen liu Zheng Wang Department of Gastrointestinal Surgery The First People’s Hospital of WuhuWuhu 241000Anhui ProvinceChina Department of Breast Surgery The First People’s Hospital of WuhuWuhu 241000Anhui ProvinceChina Department of Pathology The First People’s Hospital of WuhuWuhu 241000Anhui ProvinceChina Department of Gastrointestinal Surgery Colorectal Cancer CenterShanghai Tenth People’s Hospital Affiliated to Tongji UniversityShanghai 200000China

BACKGROUND Breast cancer(BC)metastasis to the gastrointestinal tract is uncommon,colonic metastasis from BC(CMBC)is even *** SUMMARY This report describes a 44-year-old female patient with metastatic triple-negative BC in the ascending colon who underwent laparoscopic radical right *** patient had undergone left modified radical mastectomy only 15 months ago and stopped chemotherapy just 3 months *** diagnosis of CMBC was made based on the previous history of BC and positive results of several specific immunohistochemical markers(gross cystic disease fluid protein 15,mammaglobin,GATA-binding protein 3,and cytokeratin 7)for breast *** CMBC should be highly cautious in patients with a previous history of BC,especially triple-negative BC,and further examination to aid in diagnosis.

关键词： Breast cancer Colon metastasis Colonoscopy Pathology Surgery Review Case report

Development and molecular cytogenetic identification of a new wheat-rye 6RL ditelosomic addition and 1R(1B)substitution line with powderymildew resistance

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Journal of Integrative Agriculture 2025年第1期24卷 72-84页

作者： Guohao Han Jing Wang Hanwen Yan Lijun Cao shiyu liu Xiuquan Li Yilin Zhou Wei liu Tiantian Gu Zhipeng shi Hong liu Lihui Li Diaoguo An Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of AgriculturalSciencesBeijing 100081China State Key Laboratory for Biology of Plant Disease and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193China Innovation Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Powdery mildew is a serious disease caused by Blumeria graminis ***(Bgt)that critically threatens the yield and quality of wheat(Triticum aestivum L.).Using effective powdery mildew resistance genes is the optimal method for controlling this *** the background of high genetic homogeneity among the modern commercial cultivars that are mainly derived from conventional interbreeding,the resistance genes from wheat relatives have especially prominent *** triticale,produced from common wheat and rye(Secale cereale L.)through distant hybridization,is a new synthetic species and valuable gene donor for wheat *** this study,we developed the wheat-rye line YT5 through the hybridization of octaploid triticale and two wheat ***5 was confirmed to be a 6RL ditelosomic addition and 1R(1B)substitution line using genomic in situ hybridization(GISH),multicolor fluorescence in situ hybridization(mc-FISH),multicolor GISH(mc-GISH)and molecular marker *** analysis showed that the powdery mildew resistance in YT5 was derived from the rye chromosome arm *** inoculation with different Bgt isolates at the seedling stage,YT5 had compound reaction patterns with both obvious spores and hypersensitivity,and it gradually became highly resistant until the adult-plant stage,thus showing a resistance response significantly different from the reported Pm genes from rye chromosome ***5 also showed promising agronomic performance,so it is expected to be an elite resistance donor for wheat *** promote the transfer of the chromosome arm 6RL of YT5 in marker-assisted selection(MAS)breeding,we selected and verified two 6RL-specific kompetitive allelespecific PCR(KASP)markers that can be applied to efficiently detect this chromosome arm in different wheat backgrounds.

关键词： powdery mildew common wheat Triticale 6RL ditelosomic addition line agronomic performance marker-assisted selection(MAS)

Breaking Solvation Dominance Effect Enabled by Ion-Dipole Interaction Toward Long-Spanlife Silicon Oxide Anodes in Lithium-Ion Batteries

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Nano-Micro Letters 2025年第4期17卷 367-380页

作者： Shengwei Dong Lingfeng shi Shenglu Geng Yanbin Ning Cong Kang Yan Zhang Ziwei liu Jiaming Zhu Zhuomin Qiang Lin Zhou Geping Yin Dalong Li Tiansheng Mu Shuaifeng Lou State Key Laboratory of Space Power-Sources School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001People’s Republic of China School of Marine Science and Technology Harbin Institute of Technology at WeihaiWeihai 264200People’s Republic of China Chongqing Research Institute of HIT Chongqing 401135People’s Republic of China

Micrometer-sized silicon oxide(SiO)anodes encounter challenges in large-scale applications due to significant volume expansion during the alloy/de-alloy ***,an innovative deep eutectic electrolyte derived from succinonitrile is introduced to enhance the cycling stability of SiO *** functional theory calculations validate a robust ion-dipole interaction between lithium ions(Li^(+))and succinonitrile(SN).The cosolvent fluoroethylene carbonate(FEC)optimizes the Li^(+)solvation structure in the SN-based electrolyte with its weakly solvating *** dynamics simulations investigate the regulating mechanism of ion-dipole and cation-anion *** unique Li^(+)solvation structure,enriched with FEC and TFSI^(-),facilitates the formation of an inorganic-organic composite solid electrolyte interphase on SiO ***-CT further detects the inhibiting effect on the SiO volume *** a result,the SiO|LiCoO_(2) full cells exhibit excellent electrochemical performance in deep eutectic-based *** work presents an effective strategy for extending the cycle life of SiO anodes by designing a new SN-based deep eutectic electrolyte.

关键词： Lithium-ion batteries Micrometer-sized silicon oxide Ion-dipole interaction Long-term cycling

Impact of land use change on carbon storage in the middle reaches of the Yellow River,China

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Journal of Arid Land 2025年第2期17卷 167-181页

作者： shi Xiaoliang ZHANG Jie liu Simin DING Hao CHEN Xi WANG Li ZHANG Dan College of Geomatics Xi'an University of Science and TechnologyXi'an 710054China China National Forestry-Grassland Development Research Center Beijing 100714China

The implementation of long-term shelterbelt programs in the middle reaches of the Yellow River(MRYR),China not only has improved the overall ecological environment,but also has led to the changes of land use pattern,causing carbon storage ***,the relationship between carbon storage and land use change in the MRYR is not concerned,which results in the uncertainty in the simulation of carbon storage in this *** use changes directly affect the carbon storage capacity of ecosystems,and as an indicator reflecting the overall state of land use,land use degree has an important relationship with carbon *** this study,land use data and the integrated valuation of ecosystem services and trade-offs(InVEST)model were used to assess the trends in land use degree and carbon storage in the MRYR during *** potential impact index and the standard deviation ellipse(SDE)algorithm were applied to quantify and analyze the characteristics of the impact of land use changes on carbon ***,land use transitions that led to carbon storage variations and their spatial variations were *** results showed that:(1)the most significant periods of carbon storage changes and land use transitions were observed during 1990-1995 and 1995-2020,with the most changed areas locating in the east of Fenhe River and in northwestern Henan Province;(2)the positive impact of land use degree on carbon storage may be related to the environmental protection measures implemented along the Yellow River,while the negative impact may be associated with the expansion of construction land in plain areas;and(3)the conversion of other land use types to grassland was the primary factor affecting carbon storage changes during *** future land use planning,attention should be given to the direction of grassland conversion,and focus on reasonably limiting the development of construction *** enhance carbon storage,it will be crucial to increase the area

关键词： carbon storage land use degree integrated valuation of ecosystem services and trade-offs(InVEST)model potential impact standard deviation ellipse(SDE)