Shenmai Injection Reduces Cardiomyocyte Apoptosis Induced by Doxorubicin through miR-30a/Bcl-2

作     者：ZHANG Xiao-nan LI Yan-yang LYU Shi-chao JIA Qiu-jin ZHANG Jun-ping LIU Long-tao 

作者机构：Department of Cardiovascular MedicineNational Clinical Research Center for Chinese Medicine CardiologyXiyuan HospitalChina Academy of Chinese Medical SciencesBeijing100091China Department of Integrated Traditional Chinese and Western MedicineTianjin Medical University Cancer Institute and HospitalTianjin300060China Department of Cardiovascular MedicineFirst Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjin300193China Department of Cardiovascular MedicineTianjin Key Laboratory of Traditional Research of Traditional Chinese Medicine Prescription and SyndromeTianjin300193China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志(英文版))

年 卷 期：2025年第31卷第3期

页      面：240-250页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(No.81904118) Hospital Capability Enhancement Project of Xiyuan Hospital,China Academy of Chinese Medical Sciences(No.XYZX0201-09) the Special Programme for the Cultivation of Outstanding Young Scientific and Technological Talents of the China Academy of Chinese Medical Sciences(No.ZZ17-YQ-005) 

主　　题：Shenmai Injection doxorubicin cardiotoxicity miR-30a/Bcl-2 apoptosis 

摘      要：Objective:To explore the molecular mechanism of Shenmai Injection(SMI)against doxorubicin(DOX)induced cardiomyocyte ***:A total of 40 specific pathogen-free(SPF)male Sprague Dawley(SD)male rats were divided into 5 groups based on the random number table,including the control group,the model group,miR-30a agomir group,SMI low-dose(SMI-L)group,and SMI high-dose(SMI-H)group,with 8 rats in each *** for the control group,the rats were injected weekly with DOX(2 mg/kg)in the tail vein for 4 weeks to induce myocardial injury,and were given different regimens of continuous intervention for 2 *** function was detected by echocardiography and myocardial pathological changes were observed by Van Gieson(VG)*** injury serum markers,including creatine kinase(CK),lactate dehydrogenase(LDH),troponin T(c Tn T),N-terminal pro-brain natriuretic peptide(NT-pro BNP),soluble ST2(sST2),and growth differentiation factor-15(GDF-15)were detected by enzyme linked immunosorbent assay(ELISA).Cardiomyocyte apoptosis was observed by terminal deoxynucleotidyl transferase-mediated biotinylated d UTP triphosphate nick end labeling(TUNEL)and transmission electron microscopy,and the expressions of target proteins and m RNA were detected by Western blot and quantitative real time polymerase chain reaction(qRT-RCR),***:The treatment with different doses of SMI reduced rat heart mass index and left ventricular mass index(P0.05),significantly improved the left ventricular ejection fraction(P0.05),decreased the levels of serum CK,LDH,cTnT,and NT-proBNP(P0.05 or P0.01),reduced the levels of serum sST2 and GDF-15(P0.05 or P0.01),decreased the collagen volume fraction,reduced the expressions of rat myocardial typeⅠand typeⅢcollagen(P0.05 or P0.01),and effectively alleviated myocardial *** the study found that SMI promoted the expression levels of miR-30a and Bcl-2 in myocardium,and down-regulated the expression of Bax,which inh