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Mitofusin-2 ameliorates high-fat diet-induced insulin resistance in liver of rats

World Journal of Gastroenterology 2013年第10期19卷 1572-1581页

作者： Ke-Xin Gan Chao Wang Jin-Hu Chen Chun-Jing Zhu guang-yao song Department of Internal Medicine Hebei Medical University Department of Endocrinology the General Hospital of Hebei Province

AIM:To investigate the effects of mitofusin-2(MFN2) on insulin sensitivity and its potential targets in the liver of rats fed with a high-fat diet(HFD).METHODS:Rats were fed with a control or HFD for 4 or 8 wk,and were then infected with a control or an MFN2 expressing adenovirus once a week for 3wk starting from the 9th *** glucose(BG),plasma insulin and insulin sensitivity of rats were determined at end of the 4th and 8th wk,and after treatment with different amounts of MFN2 expressing adenovirus(108,109 or 1010 vp/kg body weight).BG levels were measured by Accu-chek Active *** insulin levels were analyzed by using a Rat insulin enzymelinked immunosorbent assay *** resistance was evaluated by measuring the glucose infusion rate(GIR) using a hyperinsulinemic euglycemic clamp *** expression or phosphorylation levels of MFN2 and essential molecules in the insulin signaling pathway,such as insulin receptor(INSR),insulin receptor substrate 2(IRS2),phosphoinositide-3-kinase(PI3K),protein kinase beta(AKT2) and glucose transporter type 2(GLUT2) was assayed by quantitative real-time polymerase chain reaction and ***:After the end of 8wk,the body weight of rats receiving the normal control diet(ND) and the HFD was not significantly different(P>0.05).Compared with the ND group,GIR in the HFD group was significantly decreased(P<0.01),while the levels of BG,triglycerides(TG),total cholesterol(TC) and insulin in the HFD group were significantly higher than those in the ND group(P<0.05).Expression of MFN2 mRNA and protein in liver of rats was significantly downregulated in the HFD group(P<0.01) after 8 wk of HFD *** expression of INSR,IRS2 and GLUT2 were down-regulated markedly(P<0.01).Although there were no changes in PI3K-P85 and AKT2 expression,their phosphorylation levels were decreased significantly(P<0.01).After intervention with MFN2 expressing adenovirus for 3wk,the expression of MFN2 mRNA and protein levels we

关键词： Mitofusin-2 High-fat diet Insulin resistance Insulin pathway Liver

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Role of X-Box Binding Protein-1 in Fructose-Induced De Novo Lipogenesis in HepG2 Cells

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Chinese Medical Journal 2018年第19期131卷 2310-2319页

作者： Xian Yu Lu-Ping Ren Chao Wang Ya-Jun Zhu Han-Ying Xing Jing Zhao guang-yao song Department of Internal Medicine Hebei Medical University Shijiazhuang Hebei 050051 China Department of Endocrinology Hebei General Hospital Shijiazhuang Hebei 050051 China Hebei Key Laboratory of Metabolic Disease Hebei General Hospital Shijiazhuang Hebei 050051 China Department of Oncology Hebei General Hospital Shijiazhuang Hebei 050051 China

Background：A high consumption of fructose leads to hepatic *** 20-30% of triglycerides are synthesized via de novo *** studies showed that endoplasmic reticulum stress （ERS） is involved in this process,while others showed that a lipotoxic environment directly influences ER ***,our aim was to investigate the causal relationship between ERS and fatty acid synthesis and the effect of X-box binding protein-1 （XBP-1）,one marker of ERS,on hepatic lipid accumulation stimulated by high ***：HepG2 cells were incubated with different concentrations of *** regulators of de novo lipogenesis （i.e.,carbohydrate response element-binding protein [ChREBP] and sterol regulatory element-binding protein 1 c [SREBP-lc]） were measured by polymerase chain reaction and key lipogenic enzymes （acetyl-CoA carboxylase [ACC],fatty acid synthase [FAS],and stearoyl-CoA desaturase-1 [SCD-1]） by Western *** same lipogenesis-associated factors were then evaluated after exposure of HepG2 cells to high fructose followed by the ERS inhibitor tauroursodeoxycholic acid （TUDCA） or the ERS inducer ***,the same lipogenesis-associated factors were evaluated in HepG2 cells after XBP-1 upregulation or downregulation through cell ***：Exposure to high fructose increased triglyceride levels in a dose-and time-dependent manner and significantly increased mRNA levels of SREBP-1c and ChREBP and protein levels ofFAS,ACC,and SCD-1,concomitant with XBP-1 conversion to an active spliced ***-associated factors induced by high fructose were inhibited by TUDCA and induced by *** level in XBP-l-deficient group decreased significantly compared with high-fructose group （4.41 ± 0.54 μmol/g vs.6.52 ± 0.38 μmol/g,P 〈 0.001）,as mRNA expressions of SREBP-1c （2.92 ± 0.46 vs.5.08 ± 0.41,P 〈 0.01） and protein levels of FAS （0.53 ± 0.06 vs.0.85 ± 0.05,P =0.01）,SCD-1 （0.65 ± 0.06 vs.0.90 ± 0.04,P

关键词： Endoplasmic Reticulum Stress Fatty Liver Lipogenesis X-Box Binding Protein-I

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First molecular characterization of Giardia duodenalis from white yaks in China

First molecular characterization of Giardia duodenalis from ...

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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： guang-yao song Dong-Hui Zhou guang-Hui Zhao Si-Yuan Qin Ming-Yang Yin Xing-Quan Zhu Ming-Xin song College of Veterinary Medicine Northeast Agricultural University State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences College of Veterinary Medicine Northwest A&F University Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou University College of Veterinary Medicine

Background:Giardia duodenalis is one of the most common enteric parasites in humans and animals,including companion animals,livestock and *** date,the information about the prevalence and molecular characterization of *** infection in white yaks was ***:In the present study,a total of 208 white yak fecal samples were collected from Tianzhu Tibetan Autonomous County(TTAC) between September 2013 and March *** 208 white yak fecal samples,four samples(1.92%,all collected in March 2014) were tested G duodenalis-positive by PCR amplification of triosephosphate isomerase(TPI) ***-Joining(NJ) analysis was carried out to identify the *** assemblages in Mega 5.0 software,then only assemblage E was determined in the present ***:The present study not only revealed the presence and genotype of *** in white yaks for the first time,but also extended the host range of *** results will provide useful information for further prevention and the control of the *** infection.

关键词： Giardia duodenalis Prevalence Genotype White Yak China

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关于二阶自适应开路边条件的进一步讨论

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水动力学研究与进展（A辑） 1992年第3期7卷 350-358页

作者：邹光远陈耀松北京大学力学系北京100871

本文对二阶自适应开路边条件(以下简称AOBC2)作了进一步的讨论。指出了对局部瞬时等价波方向(±k_x,k_y)~T的选取范围应有所限制,即要求k_x^2+k_y^2/2>0,且k_x^2≠k_y^2。由于在实际运用中,波的传播方向一般不会与人为边界近于平行,故... 详细信息

本文对二阶自适应开路边条件(以下简称AOBC2)作了进一步的讨论。指出了对局部瞬时等价波方向(±k_x,k_y)~T的选取范围应有所限制,即要求k_x^2+k_y^2/2>0,且k_x^2≠k_y^2。由于在实际运用中,波的传播方向一般不会与人为边界近于平行,故这些限制不会影响本方法的使用价值。同更多的现有方法作比较,进一步证明了本方法的有效性。

关键词：开路边条件稳定性自适应

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FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression

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Neural Regeneration Research 2025年第7期20卷 2068-2083页

作者： Jing yao Yuan Li Xi Liu Wenping Liang Yu Li Liyong Wu Zhe Wang Weihong song The National Clinical Research Center for Geriatric Disease Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina Department of Pediatrics The Second Affiliated Hospital and Yuying Children’s HospitalKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceInstitute of AgingWenzhou Medical UniversityWenzhouZhejiang ProvinceChina Oujiang Laboratory(Zhejiang Laboratory for Regenerative Medicine Visionand Brain Health)WenzhouZhejiang ProvinceChina

Alzheimer's disease is characterized by deposition of amyloid-β,which forms extracellular neuritic plaques,and accumulation of hyperphosphorylated tau,which aggregates to form intraneuronal neurofibrillary tangles,in the *** NLRP3 inflammasome may play a role in the transition from amyloid-βdeposition to tau phosphorylation and *** NLRP3 is primarily found in brain microglia,and tau is predominantly located in neurons,it has been suggested that NLRP3 expressed by microglia indirectly triggers tau phosphorylation by upregulating the expression of pro-inflammatory ***,we found that neurons also express NLRP3 in vitro and in vivo,and that neuronal NLRP3 regulates tau *** biochemical methods,we mapped the minimal NLRP3 promoter and identified FUBP3 as a transcription factor regulating NLRP3 expression in *** primary neurons and the neuroblastoma cell line Neuro2A,FUBP3 is required for endogenous NLRP3 expression and tau phosphorylation only when amyloid-βis *** the brains of aged wild-type mice and a mouse model of Alzheimer's disease,FUBP3 expression was markedly increased in cortical *** analysis suggested that FUBP3 plays a role in neuron-mediated immune *** also found that FUBP3 trimmed the 5′end of DNA fragments that it bound,implying that FUBP3 functions in stress-induced *** findings suggest that neuronal NLRP3 may be more directly involved in the amyloid-β-to–phospho-tau transition than microglial NLRP3,and that amyloid-βfundamentally alters the regulatory mechanism of NLRP3 expression in *** that FUBP3 was only expressed at low levels in young wild-type mice and was strongly upregulated in the brains of aged mice and Alzheimer's disease mice,FUBP3 could be a safe therapeutic target for preventing Alzheimer's disease progression.

关键词： 5′end trimming Alzheimer's disease amyloid-beta amyloid-β-dependent transcription FUBP3 inflammasome inflammation neuron NLRP3 tau transcription factor

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车辆薄壁结构撞击吸能特性研究

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铁道机车车辆 2002年第2期22卷 8-10页

作者：高广军姚松中南大学铁道校区高速列车研究中心长沙410075

对 6种具有代表性的吸能结构进行了撞击分析 ,得到了不同的吸能结构在受到撞击时的变形模式、吸能量大小及冲击力大小等一系列参数 ,并分析各种吸能结构特性的优劣 ,为耐冲击吸能车体的实车碰撞数值模拟奠定基础。

关键词：铁道车辆薄壁吸能结构接触冲击变形模式撞击分析列车碰撞事故

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Infiltration by monocytes of the central nervous system and its role in multiple sclerosis: reflections on therapeutic strategies

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Neural Regeneration Research 2025年第3期20卷 779-793页

作者： guangyong Zhang Qing yao Chubing Long Pengcheng Yi Jiali song Luojia Wu Wei Wan Xiuqin Rao Yue Lin Gen Wei Jun Ying Fuzhou Hua Department of Anesthesiology the Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi ProvinceChina Key Laboratory of Anesthesiology of Jiangxi Province NanchangJiangxi ProvinceChina

Mononuclear macrophage infiltration in the central nervous system is a prominent feature of neuroinflammation. Recent studies on the pathogenesis and progression of multiple sclerosis have highlighted the multiple roles of mononuclear macrophages in the neuroinflammatory process. Monocytes play a significant role in neuroinflammation, and managing neuroinflammation by manipulating peripheral monocytes stands out as an effective strategy for the treatment of multiple sclerosis, leading to improved patient outcomes. This review outlines the steps involved in the entry of myeloid monocytes into the central nervous system that are targets for effective intervention: the activation of bone marrow hematopoiesis, migration of monocytes in the blood, and penetration of the blood–brain barrier by monocytes. Finally, we summarize the different monocyte subpopulations and their effects on the central nervous system based on phenotypic differences. As activated microglia resemble monocyte-derived macrophages, it is important to accurately identify the role of monocyte-derived macrophages in disease. Depending on the roles played by monocyte-derived macrophages at different stages of the disease, several of these processes can be interrupted to limit neuroinflammation and improve patient prognosis. Here, we discuss possible strategies to target monocytes in neurological diseases, focusing on three key aspects of monocyte infiltration into the central nervous system, to provide new ideas for the treatment of neurodegenerative diseases.

关键词： blood–brain barrier macrophages monocytes multiple sclerosis neuroinflammation review therapy

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无乳链球菌LIP蛋白的截短表达及免疫保护性研究

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淡水渔业 2017年第3期47卷 25-32页

作者：刘鸿艳张耀光张松林沈志强马永彪西南大学生命科学学院淡水鱼类资源与生殖发育教育部重点实验室水产科学重庆市市级重点实验室重庆400715 山东省滨州畜牧兽医研究院山东滨州256600 山东省滨州海洋环境监测站山东滨州256600

为评价罗非鱼源无乳链球菌(Streptococcus agalactiae)BX2012株脂蛋白(lipoprotein)的免疫原性及其对奥利亚罗非鱼(Oreochromis aureus)和大菱鲆(Scophthalmus maximus)的保护效果,以无乳链球菌脂蛋白基因序列(GenBank序列号:CP000114.1,SAK_0321)的B细胞线性抗原表位区设计特异性引物进行扩增,构建重组表达载体,将截短表达的脂蛋白制备成亚单位疫苗,同时制备灭活疫苗进行免疫比对。结果显示:重组表达载体p ET-32a-LIP342在BL21(DE3)中获得了良好的可溶性表达,分子质量约为30 kDa,纯化使LIP342纯度由41.75%提至87.23%;Western blotting分析显示,LIP342可被兔抗无乳链球菌高免血清特异性识别;LIP342在目前已知不同种属来源或不同血清型的无乳链球菌分离株中同源性为90.35%~100%,在罗非鱼源分离株中同源性为100%;无乳链球菌LIP342蛋白和灭活疫苗均可显著提升奥利亚罗非鱼和大菱鲆血清抗体水平,而LIP342诱导的血清抗体水平均显著高于灭活疫苗;经0.1 mL 1×10~9cfu/mL的无乳链球菌攻毒后,LIP342蛋白和灭活疫苗对供试鱼的累积存活率均显著高于PBS对照组。结果表明,高度保守的LIP342具有较好的免疫原性,可作为无乳链球菌亚单位疫苗候选因子。

关键词：无乳链球菌(Streptococcus agalactiae) 脂蛋白截短表达免疫保护奥利亚罗非鱼(Oreochromis aureus) 大菱鲆(Scophthalmus maximus)

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Impacts of the annual cycle of background SST in the tropical Pacific on the phase and amplitude of ENSO

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 12-17页

作者： song Jiang Congwen Zhu Ning Jiang State Key Laboratory of Severe Weather(LASW) Chinese Academy of Meteorological SciencesBeijingChina College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijingChina

The dominant annual cycle of sea surface temperature(SST)in the tropical Pacific exhibits an antisymmetric mode,which explains 83.4%total variance,and serves as a background of El Niño-Southern Oscillation(ENSO).However,there is no consensus yet on its anomalous impacts on the phase and amplitude of *** on data during 1982-2022,results show that anomalies of the antisymmetric mode can affect the evolution of ENSO on the interannual scale via Bjerknes feedback,in which the positive(negative)phase of the antisymmetric mode can strengthen El Niño(La Niña)in boreal winter via an earlier(delayed)seasonal cycle transition and larger(smaller)annual *** magnitude of the SST anomalies in the equatorial eastern Pacific can reach more than±0.3◦C,regulated by the changes in the antisymmetric mode based on random sensitivity *** reveal the spatial pattern of the annual cycle associated with the seasonal phase-locking of ENSO evolution and provide new insight into the impact of the annual cycle of background SST on ENSO,which possibly carries important implications for forecasting ENSO.

关键词： Annual cycle SST anomaly Antisymmetric mode ENSO

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Constraints on holographic QCD phase transitions from PTA observations

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： song He Li Li Sai Wang Shao-Jiang Wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

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