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Transfection of colorectal cancer cells with chemokine MCP-3(monocyte chemotactic protein-3)gene retards tumor growth and inhibits tumor metastasis

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World Journal of Gastroenterology 2002年第6期8卷 1067-1072页

作者： Jian-GaoZhu Yue-FeiLi Guo-HuaZhou Qu-BingSun Jin-YueHu guan-ch DepartmentofInternalMedicine HunanArmedPoliceForceHospitalChangsha410006HumanProvinceChina LaboratoryofTumorImmunobiology CancerResearchInstituteXiangyaMedicalSchoolCentralSouthUniversityChangsh

AIM: To evaluate the possibility of the induction of antitumor immune response by transfecting the colorectal cancer cells with chemokine MCP-3 gene. METHODS: Mouse MCP-3 gene was transduced into mouse colorectal cancer cells CMT93 by using of Liposome. G418-resistant clones were selected and the MCP-3 mRNA expression was detected by RT-PCR. The chemotactic activity of MCP-3 in the cell culture supernatant was detected by chemotaxis assay. The tumorigenicity of wild type CMT93 and CMT93 gene transfectants were detected by in vivo experiments. The immune cell infiltrations in tumor tissue and tumor metastasis were detected ***: MCP-3 mRNA expression was detected by RTPCR in gene-transfected cells (CMT93/MCP-3), but not in control groups. And MCP-3 secreted in the cell culture supernatant possessed chemotatic activity. The results from in vivo experiments showed that the tumorigenicity of CMT93/MCP-3 had not decreased, but the tumors derived from CMT93/MCP-3 cells grew more slowly than those from CMT93 cells (1.021±0.253) cm2 VS(1.769±0.371) cm2,P＜0.05) or CMT93/mock cells (1.021±0.253) cm2 VS(1.680±0.643)cm2, P＜0.05). Histophathological results showed few immune cells infiltrating in the tumor tissue derived from the controls. In the tumor tissue derived from CMT93/MCP-3, infiltrating immune cells increased. In addition, no tumor metastasis was found in all mice inoculated with CMT93/MCP-3 tumor cells. But all mice had tumor metastasis in CMT93 controls and 4 in 5 mice had tumor metastasis in CMT93/mock ***: The results suggested that the transfection of chemokine MCP-3 gene could promote the induction of anti-colorectal cancer immunity, but the tumor growth could not be inhibited completely by merely MCP-3 gene transfection.

关键词：结直肠癌细胞趋化因子 MCP-3 基因转染肿瘤生长肿瘤转移抑制作用

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Photochemical reaction of azobenzene in solvent-swollen Nafion membranes

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chinese Journal of chemistry 1996年第6期14卷 541-548页

作者： Tung, ch guan, JQ chINESE ACAD SCI INST PHOTOG CHEMBEIJING 100101PEOPLES R CHINA

The photochemical reactions of azobenzene (AB) incorporated into different solvent-swollen Nafion membranes were investigated. The location of azobenzene in water-swollen Nafion membranes was different from that in methanol-swollen Nafion membranes, which was responsible for the different photochemical reactions of AB. In methanol-swollen Nafion membranes only transcis isomerization took place, while in water-swollen Nafion membranes photochemical cyclization of AB occurred after rapid transcis isomerization. The relation between the relative quantum yields and the light intensity showed that the cyclization was a two-photo process. The numbers of AB molecules per cluster (n(AB)/cluster) in Nafion membranes with different AB concentrations and different water contents were calculated. Under the experimental condition two competitive photocyclization mechanisms were suggested by n(AB)/cluster in Nafion membranes, relative quantum yields of the cyclization, and the change of the ratio of benzidine to benzo[c]cinnoline.

关键词： Nafion photochemical reaction azobenzene

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Photophysical Behaviors of Azobenzene in Solvent-Swollen Nafion Membranes

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chinese chemical Letters 1996年第10期7卷 944-947页

作者： guan, JQ Tung, ch ACAD SINICA INST PHOTOG CHEMBEIJING 100101PEOPLES R CHINA

The fluorescence from azobenzene (AB) molecule incorporated into water-swollen Nafion in acid form was observed at room temperature for the first time. The incorporation process was investigated by UV absorption. The plot of the concentration of AB in Nafion vs. the soaking time shows that the adsorption process is diffusion-controlled, and the apparent diffusion coefficient (D) was determined to be 7.6x10(-10) cm(2)s(-1). These results are attributed to the fact that AB molecules are located in the fluorocarbon/water interface of the Nafion membranes and are protonated. In methanol-swollen Nafion membranes, AB fails to fluoresce because it is located at the methanol pool enclosed by the cluster networks.

关键词： Photophysical Behaviors of Azobenzene in Solvent-Swollen Nafion Membranes

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Dynamics of electrodeposition of tetraethylthioram disulphide(TETD) on pyrite surface

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Journal of Central South University 2001年第3期13卷 164-168页

作者： QIN Wen-qing,QIU guan-zhou,HU Yue-hua,XU Jing (Department of Mineral Enginering, Central South University, changsha 410083, ch ina) 1. Department of Mineral Enginering Central South University 410083 Changsha China

The electrode process of pyrite in diethyldithiocarbamate (DDTC) solution pH 11.4 was investigated by using cyclic voltammetry, potentiost atic and chronopotentiometry. Tetraethylthioram disulphide(TETD) was electrodepo sited on pyrite electrode surface as the electrode potential is higher than 0.2 V. The relationship of the current density caused by diffusion and reaction time can be ascertained as i =1/(9.08×10 -5 +4.77×10 -3 t 0.5 ) , and the diffusion coefficient of DDTC on pyrite surface is about 3.72×10 - 6 cm 2/s. At pH 11.4, the thickness of TETD adsorbed on pyr ite surface is about 1.63 molecule layer. The electrochemical dynamics equation of the reduction of TETD on pyrite surface is given as η =0.116-0.064log[1- ( t/τ ) 0.5 ]. The kinetic parameters were determined as follows: the ex change current density ( i 0) is 3.08 μA/cm 2; the transmission coeffi cient( α ) is 0.462.

关键词： electrode process pyrite electrochemistry of flotation

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High temperature thermo-photocatalysis driven carbon removal in direct biogas fueled solid oxide fuel cells

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chinese chemical Letters 2021年第11期32卷 3548-3552页

作者： Doudou Gu guan Zhang Jing Zou Institute of Hydrogen and Fuel Cell Harbin Institute of TechnologyShenzhenShenzhen 518055China General Education Division School of Humanities Social ScienceThe Chinese University of Hong KongShenzhenShenzhen 518172China School of Civil and Environmental Engineering Harbin Institute of TechnologyShenzhenShenzhen 518055China

Solid oxide fuel cells(SOFCs)can directly convert renewable biogas into electricity with high efficiency at high temperature,however the long-term stability of SOFCs is significantly affected by the carbon deposition on the anode during cell ***,we report a novel carbon removal approach by high temperature infrared light driven photocatalytic *** the comparison of electrochemical performance of Ni-YSZ anode and TiO_(2)modified Ni-YSZ anode in the state-of-the-art single cell(Ni-YSZ/YSZ/LSCM),the modified anodes exhibit markedly improved peak powder density with simulated biogas fuel(70%ch_(4)+30%CO_(2))at 850℃with less coking after 40 h *** high activity and carbon deposition resistance of the modified anode is possibly attributed to the in situ generated hydroxyl radical from the reduced TiO_(x)powder under high temperature infrared light excitation,which is supported by detailed analysis of microstructural information of anodes and the powder-based thermo-photocatalytic experiments.

关键词： Thermo-photocatalysis Infrared light excitation Carbon removal Biogas utilization Solid oxide fuel cells

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Solution-Mediated Transformation of a 1D [Zn（Im）（HIm）2（OAc）] Precursor to Several Different 3D Zn（Im）2 Frameworks

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chinese Journal of chemistry 2017年第7期35卷 1086-1090页

作者： Youjun guan Qi Shi Jinxiang Dong College of chemistry and chemical Engineering Taiyuan University of Technology Taiyuan Shanxi 030024 China

1D compound [Zn（Im）（HIm）2（OAc）] was used as a single precursor of metal and imidazole to prepare several 3-dimensional （3D） Zn（Im）2 frameworks by solution-mediated transformation. Specifically, three known topologies, zni, coi and crb （BCT） were obtained using a solution-mediated transformation with ch3OH, DMF and DMA as solvent, respectively. Structural studies by 13C MAS NMR spectroscopy and TG imply that in the transformation process from 1D compound to coi-[Zn（Im）2]·（DMF）x and crb-[Zn（Im）2]·（DMA）x （ZIF-1）, DMF and DMA solvent molecules acted as structure-directing agent and, therefore, were occluded inside the framework, respectively. In contrast, in the formation of zni-[Zn（Im）2], no solvent molecules were present in the frameworks; therefore the transformation from 1 D compound to zni was induced by temperature. Thus, solution-mediated transformation of a single precursor （1 D compound） approach was established for the synthesis of ZIFs.

关键词： zinc metal-organic framework phase transitions

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Machine learning and a computational fluid dynamic approach to estimate phase composition of chemical vapor deposition boron carbide

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Journal of Advanced Ceramics 2021年第3期10卷 537-550页

作者： Qingfeng ZENG Yong GAO Kang guan Jiantao LIU Zhiqiang FENG Science and Technology on Thermostructural Composite Materials Laboratory School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China MSEA International Institute for Materials Genome Gu’an 065500China School of Materials Science and Engineering South China University of TechnologyGuangzhou 510640China School of Mechanical Engineering Southwest Jiaotong UniversityChengdu 610031China eSchool of Mechanics and Engineering Southwest Jiaotong UniversityChengdu 610031China LMEE-UEVE UniversitéParis-SaclayEvry 91020France

chemical vapor deposition is an important method for the preparation of boron *** of the correlation between the phase composition of the deposit and the deposition conditions (temperature,inlet gas composition,total pressure,reactor configuration,and total flow rate) has not been completely *** this work,a novel approach to identify the kinetic mechanisms for the deposit composition is *** leaning (ML) and computational fluid dynamic (CFD) techniques are utilized to identify core factors that influence the deposit *** has been shown that ML,combined with CFD,can reduce the prediction error from about 25% to 7%,compared with the ML approach *** sensitivity coefficient study shows that BHCl_(2 )and BCl_(3) produce the most boron atoms,while C_(2)H_(4) and ch_(4) are the main sources of carbon *** new approach can accurately predict the deposited boron-carbon ratio and provide a new design solution for other multi-element systems.

关键词： machine learning(ML) computational fluid dynamic(CFD) chemical vapor deposition boron carbide B/C ratio kinetic mechanisms

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Understanding the Initial Decomposition Pathways of the n-Alkane/Nitroalkane Binary Mixture

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chinese Journal of chemistry 2013年第8期31卷 1087-1094页

作者： Yulei guan Ying Zhang chunhai Yi Bolun Yang Department of chemical Engineering State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University Xi'an Shannxi 710049 China

Addition of nitroalkanes into n-alkanes can lower the activation barriers of free-radical production and acceler- ate the decomposition of n-alkanes at relatively low temperatures. Four initial decomposition mechanisms of the n-butane/nitroethane binary mixture were proposed for the promoting effect and considered theoretically at the B3LYP, BB1K, BMK, MPW1K, and M06-2X levels with MG3S basis set. Energetics above was compared to high-level CBS-QB3 and G4 calculations. Calculated results confirm the feasibility of the four initial decomposition pathways：（I） the C--NO2 bond rupture of nitroethane to produce ethyl and .NO2, （II） HONO elimination from nitroethane followed by decomposition to .OH and .NO, （1II） rearrangement of nitroethane to ethyl nitrite which further dissociates into ch3ch20. and .NO, and （IV） direct hydrogen-abstraction ofnitroethane with n-butane.

关键词： free radical initial decomposition mechanism computational chemistry hydrogen-abstraction bimol-ecular

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Bimetallic Ni-Fe catalysts derived from layered double hydroxides for CO methanation from syngas

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Frontiers of chemical Science and Engineering 2017年第4期11卷 613-623页

作者： Tang, Honggui Li, Shuangshuang Gong, Dandan guan, Yi Liu, Yuan Tianjin Univ Sch Chem Engn Dept Catalysis Sci & Technol Tianjin 300072 Peoples R China Tianjin Univ Sch Chem Engn Collaborat Innovat Ctr Chem Sci & Engn Tianjin Tianjin 300072 Peoples R China

Carbon deposition and sintering of active components such as nano particles are great challenges for Ni-based catalysts for CO methanation to generate synthetic natural gas from syngas. Facing the challenges, bimetallic catalysts with different Fe content derived from layered double hydroxide containing Ni, Fe, Mg, A1 elements were prepared by co-precipitation method. Nanoparticles of Ni-Fe alloy were supported on mixed oxides of aluminium and magnesium after calcination and reduction. The catalysts were characterized by Brunner- Emmett-Teller （BET）, X-ray diffraction, hydrogen tem- perature programmed reduction, inductively coupled plasma, X-ray photoelectron spectroscopy, transmission electron microscopy and thermogravimetric techniques, and their catalytic activity for CO methanation was investigated. The results show that the Ni-Fe alloy catalysts exhibit better catalytic performance than mono- metallic catalysts except for the Ni4Fe-red catalyst. The Ni2Fe-red catalyst shows the highest CO conversion （100% at 260-350 ~C）, as well as the highest ch4 selectivity （over 95% at 280-350 ~C）, owing to the formation of Ni-Fe alloy. In stability test, the Ni2Fe-red catalyst exhibits great improvement in both anti-sintering and resistance to carbon formation compared with the Ni0Fe-red catalyst. The strong interaction between Ni and Fe element in alloy and surface distribution of Fe element not only inhibits the sintering ofnanoparticles but restrains the formation of Ni clusters.

关键词： methanation layered double hydroxide bime-tal Ni-Fe alloy sintering carbon deposition

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Electron promoted ZnO for catalytic synthesis of higher alcohols from syngas

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Green Energy & Environment 2022年第6期7卷 1390-1400页

作者： Fang Li Qian Zhang Jing Liu Nan Cui Guoqing guan Wei Huang State Key Laboratory of Clean and Efficient Coal Utilization Taiyuan University of TechnologyTaiyuan030024ShanxiChina Energy Conversion Engineering Laboratory Institute of Regional Innovation(IRI)Hirosaki University2-1-3MatsubaraAomori030-0813Japan

Direct conversion of syngas from those non-petroleum carbon resources to higher alcohols are very attractive due to the process simplicity with low energy ***,the reaction always suffers from low yield as well as low ***,effective increase of higher alcohols proportion in the product is realized by direct conversion of syngas over electronically-modulated ZnO semiconductor via Cu *** is considered that the lower Fermi level and narrower band gap of catalysts by embedding Cu^(2+)into ZnO lattice could facilitate donor reaction by boosting the process for the reactants to obtain electrons on the catalyst surface for the formation of ch_(x) species and carbon chain growth,in which the Cu doping on ZnO lattice play important role in the promotion of CO *** a result,4 mol%Cu doped ZnO exhibits a highest C_(2+) OH/ROH fraction of 48.1%.Selectivity of catalysts from straight chain alcohol is better than from branch chain alcohol,which is different from promoted Cu/ZnO based ***,over-doping of Cu(7 mol%)on ZnO results in the aggregation Cu species on ZnO surface,leading to a sharp decrease of higher alcohols proportion to 3.2%.The results shed light on the nature that a direct correlation between semiconductor Fermi level and synthesis of higher alcohols,and the semiconductor-based catalysts mainly accelerate the hydrogenation reactions by enhancing thermally excited electron transfer.

关键词： Semiconductor catalysts Electron promotion Fermi level ZnO Higher alcohols

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