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微生物发酵生产十三碳二元酸的研究

中国食品学报 2002年第1期2卷 48-52页

作者：唐如星王昌禄顾小波杨艳斐李毅天津轻工业学院天津300222 天津师范大学天津300074

以一株热带假丝酵母(Candida tropicalis)TSW_1为出发菌株,选用不同种类、不同能量、不同剂量的离子进行离子束注入处理,得到从十三碳烷烃生产十三碳二元酸的突变菌株TSW-N-35。研究了N^+和CH_3^+离子注入剂量与Candidda tropicalis存... 详细信息

以一株热带假丝酵母(Candida tropicalis)TSW_1为出发菌株,选用不同种类、不同能量、不同剂量的离子进行离子束注入处理,得到从十三碳烷烃生产十三碳二元酸的突变菌株TSW-N-35。研究了N^+和CH_3^+离子注入剂量与Candidda tropicalis存活率之间的关系。菌株的存活率均随着注入剂量的变化表现为相似的“马鞍型”,以十三碳烷烃为底物,在摇瓶条件下,十三碳二元酸(DCA13)产量达71.5g/L。研究发现,蔗糖浓度显著地影响菌体生长,其最适浓度为0.3％;尿素和丙烯酸的浓度显著地影响DCA13的产量,尿素的最适浓度为0.14％;添加0.10％丙烯酸,DCA13的产量能提高20％左右;

关键词：微生物发酵生产十三碳二元酸离子注入热带假丝酵母

^99Tc^m-半乳糖基化人血清白蛋白融合干扰素α2b的制备和生物分布

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核技术 2008年第8期31卷 633-636页

作者：周桂楼蒋孟军张荣军蔡刚明周尧远张波顾晓波叶万忠金坚曹国宪包建东周杏琴江南大学医药学院无锡214036 江苏省原子医学研究所卫生都核医学重点实验室无锡214063

用氯化亚锡(SnCl2)还原法制备99Tcm-半乳糖基化人血清白蛋白融合干扰素α2b(GHSA-IFNα2b),研究了SnCl2和GHSA-IFNα2b的用量以及溶液pH对99Tcm-GHSA-IFNα2b标记率的影响;ICR小鼠尾静脉注射99Tcm-GHSA-IFNα2b(0.286MBq/只,n=6),于5、10、15、30、60、120min取脏器和血,称重、计数,计算%ID(organ)-1和%ID·g-1;并对99Tcm-GHSA-IFNα2b的SD大鼠显像进行了研究。结果表明,在pH=3.0~4.5、GHSA-IFNα2b的用量≥0.3mg、SnCl2的用量为3~60μg时,37℃反应30min,可得标记率>90%的99Tcm-GHSA-IFNα2b,并在6h内保持基本稳定;肝脏组织中的99Tcm-GHSA-IFNα2b在注射后10min高达52.51%ID(organ)-1,能够被肝脏特异性摄取,主要通过胆道、肠道排泄;大鼠静脉注射15~30min时,即可获得清晰的肝显像。

关键词：半乳糖基化人血清白蛋白融合干扰素α2b 99^Tc^m 肝受体显像生物分布

白桦酸体外对人肝癌细胞、鼻咽癌细胞增殖及组织血管生成的影响

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山东医药 2016年第3期56卷 5-7页

作者：黄荷云周尧远张荣军蔡刚明顾晓波潘栋辉蒋孟军江苏省原子医学研究所江苏无锡214063 卫生部核医学重点实验室江苏省分子核医学重点实验室南京医科大学附属无锡人民医院

目的观察白桦酸(BA)对肿瘤细胞增殖及组织血管生成的影响。方法培养人肝癌细胞株Bel-7402、人鼻咽癌细胞株CNE、人血管内皮细胞株HMEC,分别以不同浓度的BA干预,比较Bel-7402细胞和CNE细胞增殖抑制率、凋亡率,并比较HMEC细胞增殖抑制率... 详细信息

目的观察白桦酸(BA)对肿瘤细胞增殖及组织血管生成的影响。方法培养人肝癌细胞株Bel-7402、人鼻咽癌细胞株CNE、人血管内皮细胞株HMEC,分别以不同浓度的BA干预,比较Bel-7402细胞和CNE细胞增殖抑制率、凋亡率,并比较HMEC细胞增殖抑制率、迁移能力、血管形成能力。结果 BA可抑制Bel-7402细胞、CNE细胞增殖,促进其凋亡,且呈剂量依赖性(P均<0.05);BA可抑制HMEC细胞增殖,减弱迁移能力及血管形成能力,且呈剂量依赖性(P均<0.05)。结论 BA可抑制人肝癌细胞、鼻咽癌细胞增殖及肿瘤组织血管生成。

关键词：白桦酸人肝癌细胞人鼻咽癌细胞人血管内皮细胞

Bacterial Diversity in Alpine Lakes: A Review from the Third Pole Region

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Journal of Earth Science 2019年第2期30卷 387-396页

作者： Namita Paudel Adhikari Subash Adhikari xiaobo Liu Liang Shen Zhengquan gu Key Laboratory of Alpine Ecology and Biodiversity Institute of Tibetan Plateau ResearchChinese Academy of Sciences Key Laboratory of Tibetan Environment Changes and Land Surface Processes Institute of Tibetan Plateau ResearchChinese Academy of Sciences University of Chinese Academy of Science Janapriya Multiple Campus Tribhuvan University Kathmandu Center for Research and Education CAS-TU

Microorganisms are unique among all of the living organisms because of their high population size, advanced genetic diversity, short generation time, and quick response to the small change in environmental conditions. Remote alpine lakes of the Third Pole region provide the unique habitat for microorganisms acting as a natural laboratory and offering the information about the ecological roles of microorganisms. Many researchers focused on microbial communities as well as the impact of physicochemical, biological and hydrological parameters in lakes of this region since decades but the comprehensive review focusing on bacterial diversity and the role of environmental parameters still lacks. Here we reviewed bacterial diversity in lakes of the Third Pole region by analyzing 16 S rRNA clone libraries accessed from previous research findings. A total of 5 388 bacterial 16 S rRNA gene sequences were analyzed and classified into different phylogenetic groups. The average relative abundance of dominant taxa includes Betaproteobacteria(19%), Bacteroidetes(18%), Gammaproteobacteria(16%), Actinobacteria(15%), Alphaproteobacteria(14%), Cyanobacteria(7%), and Firmicutes(5%). Several adaptational strategies were adopted by these dominant bacterial groups in order to accommodate in the respective habitat. Nevertheless, lake water properties like temperature, pH, salinity, incident UV radiation, turbidity, and nutrients also played role in bacterial diversity.

关键词： bacterial diversity alpine lakes 16S rRNA Third Pole region

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抗酸酵母遗传特性的初步研究

微生物学通报 1998年第4期25卷 213-217页

作者：王昌禄杜连祥顾晓波路福平山田修五味胜也饭村穰天津轻工业学院食品工程系天津300222 大藏省国税厅酿造研究所

研究了酿酒酵母（Saccharomycescerevisiae）单倍体突变株YNN-27-24（atrpura）对乳酸抗性产生原因及其遗传特性。结果表明，该突变株对乳酸的抗性不是由于对环境条件的适应，而是由基因突变所致。通过对A13-18（alys）和YNN-27-24进行... 详细信息

研究了酿酒酵母（Saccharomycescerevisiae）单倍体突变株YNN-27-24（atrpura）对乳酸抗性产生原因及其遗传特性。结果表明，该突变株对乳酸的抗性不是由于对环境条件的适应，而是由基因突变所致。通过对A13-18（alys）和YNN-27-24进行杂交得到的30株杂交子代的遗传特性进行分析可以看出，YNNM-27-24突变株对乳酸和潮霉素B（HygromycinB）的抗性，均由单基因控制，并且，该突变株抗L-乳酸与抗潮霉素B基因之间存在着非常紧密的连锁关系。

关键词：酿酒酵母乳酸抗性潮霉素B 遗传特性 pH值

Micro-PET在膈下逐瘀汤抑制肝细胞癌研究中的应用

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临床肿瘤学杂志 2017年第10期22卷 880-884页

作者：蔡刚明赵韬张汀荣顾晓波黄洪波江苏省原子医学研究所卫计委核医学重点实验室江苏省分子核医学重点实验室江苏无锡214063 江阴市人民医院肿瘤科 214400

目的采用micro-PET显像技术评判中药复方膈下逐淤汤对荷肝细胞癌裸鼠的抑制作用。方法 20周龄健康裸鼠12只,随机分为给药组与对照组(n=6),皮下接种人肝癌细胞Bel7402建立荷瘤模型。接种1周后每日灌胃膈下逐淤汤0.3 ml,对照组给予等体积... 详细信息

目的采用micro-PET显像技术评判中药复方膈下逐淤汤对荷肝细胞癌裸鼠的抑制作用。方法 20周龄健康裸鼠12只,随机分为给药组与对照组(n=6),皮下接种人肝癌细胞Bel7402建立荷瘤模型。接种1周后每日灌胃膈下逐淤汤0.3 ml,对照组给予等体积生理盐水,连续给药60 d。待肿瘤长至约5 mm时测量肿瘤体积,每周2次,计算瘤体积大小和抑瘤率并绘制肿瘤生长曲线。给药24、60 d后分别行^(18)F-FDG、^(18)F-RGD micro-PET扫描各一次,测定肿瘤组织摄取量、标准摄取值(SUV)及肿瘤/肌肉靶本比(T/NT)。结果接种24 d后,肿瘤生长至5 mm,给药组瘤体积(74.8 mm^3)小于对照组(78.3mm^3)。^(18)F-FDG micro-PET扫描给药组的肿瘤组织摄取量、SUV_(mean)和T/NT分别为(5.54±1.59)%ID/g、0.93±0.20和8.20±2.52,略小于对照组(5.92±1.23)%ID/g、1.00±0.19和8.71±2.36(P>0.05)。^(18)F-RGD micro-PET扫描给药组的肿瘤组织摄取量、SUV和T/NT分别为(4.08±0.64)%ID/g、0.75±0.08和6.91±0.72,略小于对照组(4.61±1.08)%ID/g、0.87±0.16和7.00±1.40(P>0.05)。接种60 d后,给药组和对照组的肿瘤体积分别增长至1854.4 mm^3和1462.9 mm^3。^(18)F-FDG micro-PET扫描给药组的肿瘤组织摄取量、SUV和T/NT分别为(3.56±0.54)%ID/g、0.70±0.09和4.91±0.92(n=5),大于对照组的3.28%ID/g、0.60和3.98(n=1)。^(18)F-RGD micro-PET扫描给药组的肿瘤组织摄取量、SUV和T/NT分别为(2.19±0.16)%ID/g、0.51±0.04和4.15±0.57(n=4)。给药组和对照组的肿瘤重量分别为(1.93±0.95)g(n=5)和1.69 g(n=1),抑瘤率为-14.76%。给药组的中位生存时间为60 d,长于对照组的40.5 d,差异有统计学意义(P<0.05)。结论膈下逐淤汤可能通过抑制葡萄糖的吸收和新生血管的生成来延长荷肝细胞癌裸鼠的生存时间。Micro-PET能动态地定量分析活体动物模型的生理、生化变化,可用于评价抗肿瘤药物的体内疗效。

关键词：肝细胞癌膈下逐淤汤微型正电子断层扫描仪 ^18F-FDG ^18F-RGD 靶本比

23-羟基白桦酸对血管内皮细胞增殖和迁移的影响

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中药药理与临床 2006年第6期22卷 10-12页

作者：周尧远蒋孟军蔡刚明张荣军杨敏范俊张波顾晓波曹国宪江苏省原子医学研究所卫生部核医学重点实验室无锡214063

目的:探讨23-羟基白桦酸(23-HBA)对血管内皮细胞(ECV-304)形态变化,增殖和迁移的影响。方法:SRB方法测定23-HBA对ECV-304细胞的增殖抑制率,体外琼脂法检测内皮细胞的迁移能力,并用光镜观察内皮细胞形态变化。结果:23-HBA对ECV-304细胞的... 详细信息

目的:探讨23-羟基白桦酸(23-HBA)对血管内皮细胞(ECV-304)形态变化,增殖和迁移的影响。方法:SRB方法测定23-HBA对ECV-304细胞的增殖抑制率,体外琼脂法检测内皮细胞的迁移能力,并用光镜观察内皮细胞形态变化。结果:23-HBA对ECV-304细胞的IC50为39.74μg/ml。当23-HBA浓度提高到20μg/ml以上,ECV-304细胞的凋亡现象非常明显。23-HBA浓度在1μg/ml时即能抑制细胞的迁移。结论:23-HBA对ECV-304增殖和迁移均有显著抑制作用。

关键词： 23-羟基白桦酸内皮细胞增殖迁移

Gradient honeycomb metastructure with broadband microwave absorption and effective mechanical resistance

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Nano Materials Science 2024年第4期6卷 456-466页

作者： Dongmeng Li Xia Zheng Hao gu xiaobo Xuan Bing Liu Hongquan Feng Fuheng Zhang Shanghai Radio Equipment Research Institute Shanghai 200438People's Republic of China

Multifunctional metastructure integrated broadband microwave absorption and effective mechanical resistance has attracted much ***,multifunctional performance is limited by the lack of theoretical approaches to integrated ***,a multi-layer impedance gradient honeycomb(MIGH)was designed through theoretical analysis and simulation calculation,and fabricated using 3D printing technique.A theoretical calculation strategy for impedance gradient structure was established based on the electromagnetic parameter equivalent method and the multi-layer finite iterative *** impedance of MIGH was analyzed by the theoretical calculation strategy to resolve the broadband *** loss mechanism of matrix materials and distributions of electric fields,magnetic fields and power loss were analyzed to investigate the absorption *** results indicated that a 15 mm thick designed metastructure can achieve the absorption more than 88.9%in the frequency range of 2-18 ***,equivalent mechanical parameters of MIGH was calculated by integral method according to the Y-shaped *** Element analysis of stress distributions were carried out to predict the deformation *** tests demonstrate that MIGH achieved the compression modulus of 22.89 MPa and flexure modulus of 17.05 *** integration of broadband electromagnetic absorption and effective mechanical resistance was achieved by the proposed design principle and fabrication methodology.

关键词： Multifunctional metastructure Broadband microwave absorption Impedance gradient calculation Mechanical characteristics Finite element analysis

A new method for optimal FTU placement in distribution network under consideration of power service reliability

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Science China(Technological Sciences) 2017年第12期60卷 1885-1896页

作者： XU JunJun WU ZaiJun YU XingHuo HU QinRan ZHU ChengZhi DOU xiaobo gu Wei WU Zhi School of Electrical Engineering Southeast University Nanjing 210096 China School of Engineering Royal Melbourne Institute of Technology University Melbourne VIC 3001 Australia School of Engineering and Applied Science Harvard University Cambridge MA 02138 USA Zhejiang Electric Power Company Hangzhou 310014 China

Modern distribution network with high penetration of intermittent renewable sources and the so-called prosumers requires more reliable distribution automation(DA) system for safe operation and control. The design of optimal feeder terminal units(FTU)placement is critical and economical for the effective DA application. Previously proposed solutions of optimal FTU placement aiming to ensure the accuracy of state estimation(SE), typically include the following two main shortcomings: 1) only to obtain the optimal FTU placement in quantity, and the analysis of FTU location is not considered yet; 2) few consider the uncertainty of intermittent power injections in the analysis of state estimation. In this paper, a modified methodology of FTU placement is proposed not only aiming to ensure the accuracy of state estimation with the minimum number of meters, but also finding those specific FTU locations to guarantee the power service reliability. Moreover, the uncertainty models of those intermittent power injections are also considered by using probability density function(PDF). The resultant optimization problem is addressed by using the covariance matrix adaptation evolution strategy(CMA-ES). Case studies demonstrate the feasibility and effectiveness of the proposed methodology.

关键词： intermittent renewable sources FTU placement state estimation power service reliability