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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2 , GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese academy of sciences, Wuhan 4.0072, china 2. graduate school of Chinese academy of sciences, beijing 100039, china) [a]State key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China China [b]graduate school of Chinese academy of sciences Beijing China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2-year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602 amino acids, including an extracellular ligand-binding domain of 24. amino acids (aa), a single transmembrane domain of 24.aa and an intracellular signal-transduction domain of 334.aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2-year common carp, a 224.bp product was identified in liver and a 321 bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 321 bp fragment could be amplified from all the tissues of 4.month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2-year common carp and 4.month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2-year common carp was significantly lower than that of 4.month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

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Analysis and application of automatic deformation monitoring data for buildings and structures of mining area

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中国有色金属学会会刊：英文版 2011年第S3期21卷 516-522页

作者： XIAO Jie1, 2, 3, ZHANG Jin4.1. Institute of Geodesy and Geophysics, Chinese academy of sciences, Wuhan 4.0077, china 2. Key Laboratory of Dynamic Geodesy, Institute of Geodesy and Geophysics, Chinese academy of sciences, Wuhan 4.0077, china 3. graduate school of Chinese academy of sciences, beijing 10004., china 4. Department of Surveying and Mapping, Taiyuan University of Technology, Taiyuan 030024. china Taiyuan Univ Technol Dept Surveying & Mapping Taiyuan 030024 Peoples R China Chinese Acad Sci Inst Geodesy & Geophys Key Lab Dynam Geodesy Wuhan 430077 Peoples R China Chinese Acad Sci Grad Sch Beijing 100049 Peoples R China

The buildings and structures of mines were monitored automatically using modern surveying technology. Through the analysis of the monitoring data, the deformation characteristics were found out from three aspects containing points, lines and regions, which play an important role in understanding the stable state of buildings and structures. The stability and deformation of monitoring points were analysed, and time-series data of monitoring points were denoised with wavelet analysis and Kalman filtering, and exponent function and periodic function were used to get the ideal deformation trend model of monitoring points. Through calculating the monitoring data obtained, analyzing the deformation trend, and cognizing the deformation regularity, it can better service mine safety production and decision-making.

关键词： wavelet analysis Kalman filtering deformation monitoring data analysis mine

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Bio-regeneration of p-complexation desulfurization adsorbents

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Science china Chemistry 2005年第6期48卷 538-544页

作者： LI Wangliang1,2, XING Jianmin1, XIONG Xiaochao1,2, SHAN Guobin1,2 & LIU Huizhou1 1. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese academy of sciences, beijing 100080, china 2. {4. of the Chinese academy of sciences,beijing 100039, china 1. Laboratory of Separation Science and Engineering Institute of Process Engineering Chinese Academy of Sciences 100080 Beijing China 2. graduate school of the Chinese academy of sciences 100039 Beijing China

The coupling of adsorption desulfurization and biodesulfurization is a new approach to produce clean fuels. Sulfur compounds are firstly adsorbed on adsorbents, and then the ad-sorbents are regenerated by microbial conversion. p-Complexation adsorbent, Cu(I)-Y, was ob-tained by ion exchanging Y-type zeolite with Cu2+ and then by auto-reduction in helium at 4.0℃ for 3 h. Dibenzothiophene (DBT) was used as a model compound. The effects of cell concentra-tion, volume of oil phase, the ratio of aqueous phase to adsorbent on DBT desorption by a bac-terium were studied. The amounts of DBT desorbed and 2-HBP produced can be apparently increased with addition of n-octane. BDS activity can be improved by increasing cell concentra-tion and the ratio of water-to-adsorbent. 89% of DBT desorbed from the adsorbents can be converted to 2-HBP within 6 h and almost 100% within 24.h, when the volume ratio of oil-to-water was 1/5 mL/mL, the cell concentration was 60 g·L?1, and the ratio of adsorbent-to-oil was 0.03 g·mL?1. The amount of 2-HBP produced was strongly dependent on the volume ratio of oil-to- water, cell concentration and amount of adsorbent. Adsorption capacity of the regenerated ad-sorbent is 95% that of the fresh one after being desorbed with Pseudomonas delafieldii R-8, washed with n-octane, dried at 100℃ for 24.h and auto-reduced in He.

关键词： adsorption desulfurization p-complexation adsorbents bio-regeneration Pseudomonas delafieldii desorption.

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A Facile Synthetic Method for (3Z,6Z,9S,10R)-9,10-Epoxy-3,6-heneicosadiene,Sex Pheromone Component of Hyphantria Cunea (Drug)

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Chinese Chemical Letters 2005年第4期16卷 468-470页

作者： Chao CHE, Zhong Ning ZHANG StateKey Laboratory IntegratedManagementofInsectsandRodents,Institute of Zoology, Chinese academy of sciences, beijing 100080 graduate school of the Chinese academy of sciences, beijing 100039 StateKey Laboratory ofIntegratedManagementofInsectsandRodents Institute of ZoologyChinese Academy of Sciences graduate school of the Chinese academy of sciences

Total synthesis of (3Z, 6Z, 9S, 10R)-9, 10-epoxy-3, 6-heneicosadiene, sex pheromone component of Hyphantria cunea (Drug), was achieved using Sharpless AE kinetic resolution and alkylative epoxide rearrangement as ke... 详细信息

关键词： Sex pheromone Hyphantria cunea (Drug) Sharpless kinetic resolution asymmeric synthesis.

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Land cover changes based on plant successions: Deforestation, rehabilitation and degeneration of forest in the upper Dadu River watershed

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Science china Earth sciences 2005年第12期48卷 2214-2230页

作者： YAN Jianzhong1,2,3, ZHANG Yili1, BAI Wanqi1, LIU Yanhua4. BAO Weikai5, LIU Linshan1, 2 & ZHENG Du1 1. Institute of Geographic sciences and Natural Resources Research, Chinese academy of sciences, beijing 100101,{4. 2. graduate school of the Chinese academy of sciences, beijing 100039,{4. 3. College of Resources and Environment, Southwest Agricultural University, Chongqing 4.0716,{4. 4. The Ministry of Science and Technology, P. C.{4., beijing 100862,{4. 5. Chengdu Institute of Biology, Chinese academy of sciences, Chengdu 61004.,{4. 1. Institute of Geographic sciences and Natural Resources Research Chinese Academy of Sciences 100101 Beijing China 2. graduate school of the Chinese academy of sciences 100039 Beijing China 3. College of Resources and Environment Southwest Agricultural University 400716 Chongqing China 4. The Ministry of Science and Technology P. C. China 100862 Beijing China 5. Chengdu Institute of Biology Chinese Academy of Sciences 610041 Chengdu China

To link regional land use/cover changes with environmental effects, land cover changes are required to reflect vegetation successions, whereas the land cover classification systems commonly used nowadays cannot serve this purpose. In this paper, a new land cover classification system is established in which land covers are classified by the vegetation succes-sions, taking Zamtang County, Barkam County and Jinchuan County in the upper Dadu River watershed as a study area. Using multi-temporal remote sensing images, the land cover data of 1967, 1986 and 2000 are obtained by means of integration of unsupervised classification and visual interpretation methods. The database facilitates the study of land use/cover changes, en-vironmental effects and ecological construction. Land cover changes reflect the main ecological processes in the upper Dadu River watershed. The landscape composed mainly of grasslands, wildwoods and alpine scrubs in 1967 was changed to that of grasslands, secondary forests, al-pine scrubs, fragmentary wildwoods, artificial forests, secondary scrubs in 2000, meanwhile, the landscape got more fragmentized. The total area of the forests decreased by 9.4.%. Study results have shown the process of restoration of logged areas in forest centers. From 1967 to 2000, only 6.86 percents of logged areas were converted to shrubs, meadows or crop-lands, and the rest were converted into artificial forests or secondary forests. So the ecological shelter functions will be restored, stage by stage. Firewood collection, charcoal production and overgrazing are the three major triggers for the extensive degradation of alpine oak forests, Sa-bina tibetica forests and meadows. The arid valley grasslands expanded too. The degradation of vegetation in the southern slopes impairs ecological shelter functions and affects livelihood of local residents, so it is essential to find effective measures for ecological restoration and recon-struction. Field investigations have found

关键词： land use/cover classification, upper Dadu River watershed, vegetation successions, GIS, RS.

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SAR IMAGING SIMULATION OF HORIZONTAL FULLY TWO-DIMENSIONAL INTERNAL WAVES

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Journal of Hydrodynamics 2006年第3期18卷 294-302页

作者： SHEN Hui HE Yi-Jun Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China graduate school of the Chinese academy of sciences Beijing 100039 China

Based on the research of Lynett and Liu, a new horizontal fully two-dimensional internal wave propagation model with rotation effect was deduced, which can be used to simulate the characteristics of internal waves in a horizontal fully two-dimensional plane. By combining the imaging mechanism of Synthetic Aperture Radar（SAR）, a simulation procedure was fatherly acquired, which can simulate the propagation characteristics of oceanic internal waves into SAR images. In order to evaluate the validity of the proposed simulation procedure, case studies are performed in South china Sea and results from simulation procedure are analyzed in detail. A very good consistency was found between the simulation results and satellite images. The proposed simulation procedure will be a possible foundation for the quantitative interpretation of internal waves from fully two-dimensional satellite images.

关键词： oceanic internal waves imaging simulation,Synthetic Aperture Radar (SAR)

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Effect of sediment resuspension on underwater light field in shallow lakes in the middle and lower reaches of the Yangtze River:A case study in Longgan Lake and Taihu Lake

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Science china Earth sciences 2006年第z1期49卷 114-125页

作者： ZHANG Yunlin, QIN Boqiang, ZHU Guangwei, GAO Guang, LUO Liancong & CHEN Weimin Nanjing Institute of Geography & Limnology, Chinese academy of sciences, Nanjing 210008, china graduate school of Chinese academy of sciences, beijing 100039, china 1. Nanjing Institute of Geography & Limnology Chinese Academy of Sciences Nanjing 210008 China 2. graduate school of Chinese academy of sciences Beijing 100039 China

Based on three continuous in situ underwater light field measurement under different wind waves conditions in Longgan Lake, Meiliang Bay of Taihu Lake in July 2003 and littoral zone near TLLER in July 2004. respectively, the effects of sediment resuspension caused by wind waves on PAR diffuse attenuation, absorption coefficients and euphotic depths are analyzed. In Longgan Lake, PAR diffuse attenuation coefficients during small, middle and large wind waves were 1.74. 2.02 and 2.4. m-1, respectively, and the corresponding PAR spectral diffuse attenuations ranged from 0.98 to 2.97, 1.34.to 3.95 and 1.80 to 5.4. m-1, respectively. In Meiliang Bay, PAR diffuse attenuation coefficients were 2.63, 3.72, 4.37 m-1 during small, middle and large wind waves. PAR diffuse attenuation coefficients increased by 4.% and 66% from small to middle, large wind waves, respectively. Absorption coefficients integrated over the range of PAR of CDOM, phytoplankton were 0.26, 0.28 m-1; 0.76, 0.4. m-1, respectively during middle and large wind waves. Absorption coefficients integrated over the range of PAR of non-algal particulate matter and total suspended particulate matter increased from 0.94.to 1.73 m-1, and from 1.70 to 2.22 m-1, respectively during middle and large wind waves. Relative contributions of absorption coefficients of non-algal particulate matter to total absorption coefficient integrated over the range of PAR were 4..14., 65.05%, respectively, during middle and large wind waves. PAR euphotic depths decreased by 0.4., 0.19, 0.20 m from middle to large wind waves in Longganhu Lake, Meliang Bay and littoral zone near TLLER. Significant correlations were found between transparency, PAR diffuse attenuation coefficients, euphotic depths and total suspended paniculate matter, wind velocity, wave height. Most significant correlations were found between transparency, PAR diffuse attenuation coefficients, euphotic depths and inorganic suspended paniculate matter but low correlations

关键词： shallow lake, Taihu Lake, diffuse attenuation coefficient, absorption coefficient, total suspended particulate matter.

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A Test on Different Types of the Time Curve of Hardness Ratio of Gamma-Ray Bursts based on the Curvature Effect

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Chinese Journal of Astronomy and Astrophysics 2008年第4期8卷 451-464页

作者： Lan-Wei Jia National Astronomical Observatories/Yunnan Observatory,Chinese academy of sciences,Kunming 650011,china graduate school of Chinese academy of sciences,beijing 10004.,china National Astronomical Observatories/ Yunnan Observatory Chinese Academy of Sciences Kunming 650011 China and graduate school of Chinese academy of sciences Beijing 100049 China

We analyzed a sample of 66 gamma-ray bursts （GRBs） and statistically confirmed the prediction on the time curve of the hardness ratio of GRBs made by Qin et al. based on the curvature effect. In their analysis, GRB pulses are divided into three types according to the shape of their raw hardness ratio （RHR） time curves, defined as to include the background counts to the signal counts, so as to make use of counts within small time intervals. Of the three types, very hard sources exhibit a perfect pulse-like profile （type 1）, hard bursts possess a pulse-like profile with a dip in the decay phase （type 2）, and soft bursts show no pulse-like profile but have only a dipped profile （type 3）. In terms of the conventional hardness ratio, type 3 sources are indeed generally softer than those of type 1 and type 2, in agreement with the prediction. We found that the minimum value of RHR is sensitive in distinguishing the different types. We propose that GRB pulses can be classified according to the minimum value of RHR and that the different type sources may be connected with different strengths of the shock or/and the magnetic field.

关键词： gamma-rays burst -- gamma-rays observations - methods statistical

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Theoretical critical value curve and driving force formation of ecological migration in the arid land

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Chinese Science Bulletin 2006年第A01期51卷 196-203页

作者： ZHU Zi’an & ZHANG Xiaolei Xinjiang Institute of Ecology and Geography, Chinese academy of sciences, Urumqi 830011, china The graduate school of the Chinese academy of sciences, beijing 100039, china Xinjiang Uygur Autonomous Region Development and Reform Commission, Urumqi 830002, china Chinese Acad Sci Xinjiang Inst Ecol & Geog Urumqi 830011 Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China Xinjiang Uygur Autonomous Reg Dev & Reform Commis Urumqi 830002 Peoples R China

干旱陆地的易碎的 eco 环境的特征确定它骚乱抵抗的能力更低。在干旱陆地的自然荒芜的绿洲在在宽 Gobi 沙漠散布的马赛克补丁。人口分发是极大地依赖于水资源。人口与驱散的分发，简单生产和生活被描绘风格，和贫穷和遥远。生态的迁居... 详细信息

干旱陆地的易碎的 eco 环境的特征确定它骚乱抵抗的能力更低。在干旱陆地的自然荒芜的绿洲在在宽 Gobi 沙漠散布的马赛克补丁。人口分发是极大地依赖于水资源。人口与驱散的分发，简单生产和生活被描绘风格，和贫穷和遥远。生态的迁居被执行的原因躺在生态的问题。生态的降级是生态的迁居的主要驱动力。然后，驱动力的力量取决于生态的降级的度。因此，是否执行生态的移植取决于生态的降级的程度。理论上，为在干旱陆地的精明的生态的移植的批评的值曲线通过生态学的相对问题的全面研究被提出，经济等等，在干旱陆地与生态的移植经验结合了，并且基于干旱环境的特征。在这篇文章，与这条曲线，理论研究和生态的迁居的一些实践从不做作的行为和生态的移植的驱动力形成的政府行为的观点被做了。它分析活跃驱动力(因素) 和否定力量(因素) ，并且指出预定和在干旱陆地实现生态的迁居的步骤。理论曲线表达某些创见和适用性，它为为实现生态的迁居工程评估生态的降级和理论底的度提供一个量的方法。

关键词：生态迁移临界值驱动力贫瘠地

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Structures and physical properties of rigid polyurethane foams with water as the sole blowing agent

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Science china Chemistry 2006年第4期49卷 363-370页

作者： LI Xiaobin1,2, CAO Hongbin1 & ZHANG Yi1 1. Institute of Process Engineering, Chinese academy of sciences, beijing 100080, china 2. {4. of the Chinese academy of sciences, beijing 100039, china 1. Institute of Process Engineering Chinese Academy of Sciences Beijing 100080 China 2. graduate school of the Chinese academy of sciences Beijing 100039 China

Rigid polyurethane foams blown by varying water level were prepared in this study. The structures and physical properties of rigid polyurethane foams blown by water were measured with FT-IR, universal testing machine (Instron3365), scanning electron microscope (SEM) and differential scanning calorimeter (DSC). The results show that polyurea and polybiuret were the typical charac- teristics, and the cream time and gel time were shorter for the fully water blown rigid foams than that for the fully cyclopentane blown foams. The density of foam samples decreased from 4..0 kg/m3 to 27.4.kg/m3 with the increase of water level from 3 pph to 7 pph. Compressive strength exhibited the similar behavior with density. The average cell size of foam samples ranged from 24. μm to 356 μm with the increase of water level from 3 pph to 7 pph, respectively. At the same time, poorer dimen- sional stability was encountered with the increase of average cell size due to fast diffusion rate of CO2 out of the foam. The results of thermal analysis show that the glass transition temperature (Tg) shifted to a higher temperature with the increase of isocyanate level when more water was used as chemical blowing agent.

关键词： polyether polyol, polyurethane rigid foam, compressive strength, dimensional stability, cell size, glass transition temperature.

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