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Enterococcus faecalis can be distinguished from Enterococcus faecium via differential susceptibility to antibiotics and growth and fermentation characteristics on mannitol salt agar

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Frontiers in Biology 2012年第2期7卷 167-177页

作者： Maria L. G. QUILOAN John VU John carvalho Biology Program Biology Department California State University Dominguez Hills （CSUDH） Carson CA 90747 USA M. S. Biology Program Biology Department California State University Dominguez Hills （CSUDH） Carson CA 90747 USA Biology Department California State University Dominguez Hills （CSUDH） Carson CA 90747 USA

Enterococcus faecalis and Enterococcus faecium are both human intestinal colonizers frequently used in medical bacteriology teaching laboratories in order to train students in bacterial identification. In addition, hospitals within the United States and around the world commonly isolate these bacteria because they are a cause of bacteremia, urinary tract infections, endocarditis, wound infections, and nosocomial infections. Given that enterococci are becoming more of a world health hazard, it is important for laboratories to be able to distinguish these bacteria within hospitalized patients from other bacterial genera. In addition, laboratories must differentiate different species within the Enterococcus genus as well as different strains within each species. Though enterococci are differentiated from other bacterial genera via classical culture and biochemical methods, nucleic acid sequencing is required to differentiate species within the genus--a costly, time consuming, and technically challenging procedure for laboratory technicians that, in itself, does not necessarily lead to speedy identification of bactericidal antibiotics. In this study, we perform antibiogram analysis to show （1） that penicillin can be rapidly employed to distinguish strains and clinical isolates of E. faecalis from E. faecium, （2） E. faecalis is susceptible to ampicillin, and （3） that vancomycin resistance in enterococci shows no sign of abating. Additionally, we show that E. faecalis can grow on mannitol salt agar and ferment mannitol, while E. faecium lacks these phenotypes. These data reveal that we now have rapid, cost effective ways to identify enterococci to the species, and not just genus, level and have significance for patient treatment in hospitals.

关键词： enterococci Staphylococcus antibiotic resistance bacteriology microbiology

Development of a short duration method to assess the envelope thermal performance of multi-family housings

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Building Simulation 2023年第4期16卷 527-545页

作者： Lorena de carvalho Araujo Simon Thébault Laurent Mora Thomas Recht Alain Sempey Patrick Schalbart Michae Cohen CSTB 84 Av.Jean JauresChamps-sur-Marne77420France Bordeaux University 351 Cours de la LiberationTalence33405France MINES ParisTech Centre for Energy Efficiency of Systems5 rue Léon BlumPalaiseau91120France

Building energy efficiency is a key factor in reducing CO_(2) *** this reason,European Union(EU)member states have developed thermal regulations to ensure building thermal *** results are often based on results achieved with building simulation software during the design ***,the actual thermal performance can deviate significantly from the predicted one,and this difference is known as the energy performance *** indicators of the actual thermal performance are a valuable tool to guarantee building *** indicators,including the heat transfer coefficient(HTC)and the heat loss coefficient(HLC),can be estimated by the application of in situ *** multi-family housing and tertiary sector buildings are an important part of the building stock,mature methods to measure their thermal performance are *** paper presents a short-duration method for assessing the HTC in large building typologies using a sampling *** method was applied in a four-storey building model under different conditions to study the limits of the method and to improve indicator bias and *** quality was strongly influenced by the external weather conditions,the temperature variation during the protocol and the heat exchange with the adjacent *** winter conditions and with stable indoor temperatures,the method had a high accuracy when the protocol was applied for half a *** is recommended that the protocol be used over two days to improve indicator quality under less favorable test conditions.

关键词： building envelope thermal performance HTC estimation multi-family housing

Imbalance in T-cell differentiation as a biomarker of chronic diabetic foot ulceration

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Cellular & Molecular Immunology 2019年第10期16卷 833-834页

作者： Joao Moura Joao Rodrigues Marta Gonçalves Claudia Amaral Margarida Lima Eugenia carvalho Center for Neuroscience and Cell Biology(CNC) University of CoimbraCoimbraPortugal INEB-Instituto Nacional de Engenharia Biomedica University of PortoPortoPortugal I3S-Instituto de Investigaçao e Inovaçao em Saude University of PortoPortoPortugal Department of Hematology Hospital de Santo Antonio(HSA)Centro Hospitalar Universitario do Porto(CHUP)PortoPortugal Unidade Multidisciplinar de Investigaçao Biomedica(UMIB/ICBAS/UP) PortoPortugal Department of Endocrinology Hospital de Santo Antonio(HSA)Centro Hospitalar Universitário do Porto(CHUP)PortoPortugal Department of Geriatrics University of Arkansas for Medical Sciences(UAMS)and Arkansas Children’s Hospital Research Institute(ACHRI)Little RockArkansas 72202USA

Diabetes is a growing epidemic due to the systematic increase in overweight and obesity,and chronic diabetic foot ulcers(DFUs)are the most debilitating and costly complication of *** is still essential for DFU care,but early detection is limited because we do not have a good DFU ***,we report the preliminary results of a large longitudinal study to test the effectiveness of the use of T-cell phenotype and TCR repertoire diversity as DFU *** results show that the ratio of effector/naïve T-cells and the number of TCR expansions are valuable biomarkers of DFU prognosis.

关键词： diabetic chronic obesity

Are extrafloral nectaries efficient against herbivores? Herbivory and plant defenses in contrasting tropical species

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Journal of Plant Ecology 2020年第4期13卷 423-430页

作者： Cássio Cardoso Pereira Maria Gabriela Boaventura Gislene carvalho de Castro Tatiana Cornelissen Departamento de Genética Ecologia e EvoluçãoUniversidade Federal de Minas Gerais31270-910 Belo HorizonteMGBrazil Departamento de Ciências Naturais Universidade Federal de São João del-Rei36301-160 São João del-ReiMGBrazil

Aims Plants have limited resources for defenses and species that invest in biotic defenses might exhibit leaves that invest less in other types of *** have investigated whether plants that have few mechanical defenses,but have extrafloral nectaries(EFNs)patrolled by ants,are less prone to herbivory,compared with plants without EFNs that have tougher *** Data from the literature were extracted to examine the reported levels of herbivory in plants with or without *** a savanna vegetation in southern Brazil,field data were collected in leaves from six tropical species and herbivory and specific leaf area(SLA)levels were *** further evaluated differences in herbivory and SLA among species and between plants with or without *** order to test the relationship between herbivory and leaf toughness we regressed average herbivory and average SLA per *** Findings Plants exhibited variable levels of leaf damage,but plants without ant defenses experienced the highest levels of leaf area loss to *** of mechanical defenses were also variable among the plant *** without EFNs were tougher,exhibiting lower values of *** plants without EFNs had more sclerophyllous leaves,this mechanical defense was not sufficient to impair and/or reduce herbivore feeding,suggesting that the biotic defenses performed by patrolling ants might be more effective than investment in mechanical defenses associated with leaf palatability.

关键词： ants biotic defenses herbivory leaf palatability specific leaf area

Non-alcoholic fatty liver disease and the impact of genetic,epigenetic and environmental factors in the offspring

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World Journal of Gastroenterology 2022年第25期28卷 2890-2899页

作者： Natalia Balassiano Wajsbrot Nathalie carvalho Leite Gil F Salles Cristiane A Villela-Nogueira Division of Hepatology Clementino Fraga Filho University HospitalFederal University of Rio de JaneiroRio de Janeiro 20941-913Brazil Department of Internal Medicine Medical SchoolFederal University of Rio de JaneiroRio de Janeiro 22750-240Brazil

Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver disease worldwide and is strongly associated with metabolic *** recently,a significant impact of parental NAFLD in the offspring was demonstrated and has been widely ***,pathogenetic pathways implicated in the inheritance by the offspring and relatives are still under ***,multiple mechanisms are involved as well as in NAFLD pathogenesis *** the multifactorial involved mechanisms,genetic,epigenetic and environmental backgrounds are strongly related to NAFLD development in the ***,based on recent evidence from the available literature concerning genetic,epigenetic and environmental disease modifiers,this review aimed to discuss the relationship between parental NAFLD and its impact on the offspring.

关键词： Steatosis Genetic Epigenetic Environmental Offspring Non-alcoholic fatty liver disease

Delay Times and Clinical Outcomes in Acute Myocardial Infarction: Comparison of Periods before and during the COVID-19 Pandemic—Myocardial Infarction and the Pandemic

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World Journal of Cardiovascular Diseases 2024年第6期14卷 392-400页

作者： Clarice Teixeira Silva Araújo Arnon Salviato Mameri Thúlio Carrera Guarçoni Venturini Marcus Tadeu Aguilar Constantino Matos Igor Morais Araujo Lopes Luiz Gustavo Ribeiro de carvalho Murad Cleberson Duartes Ovani Guilherme Vassalo Morais Fernanda Venturini de Castro Danielle Lopes Rocha Lucas Crespo de Barros Rodolfo Costa Sylvestre Luiz Fernando Machado Barbosa Roberto Ramos Barbosa Internal Medicine Universidade Federal do Esprito Santo (UFES) Vitria Brazil Hospital Santa Casa de Misericrdia de Vitria Vitria Brazil Hospital Santa Rita de Cssia Vitria Brazil Hospital Estadual Dr. Jayme Santos Neves Serra Brazil Grupo Kora Sade Vitria Brazil Hospital Estadual Infantil e Maternidade Alzir Bernardino Alves (HIMABA) Vila Velha Brazil

Introduction: At the beginning of the COVID-19 pandemic, a drop in the number of patients treated for cardiac emergencies raised concern about cardiovascular mortality in that period. An increase in care delay for patients with ST-segment elevation myocardial infarction (STEMI) may have affected clinical outcomes. Objectives: To analyze delay times and clinical outcomes of patients with STEMI undergoing primary percutaneous coronary intervention (PPCI), before and during the COVID-19 pandemic. Methods: Retrospective observational study that included patients with STEMI undergoing PPCI from December 2018 to July 2021. The COVID-19 pandemic cases were divided into two groups: pandemic I—from March to August 2020;and pandemic II—from September 2020 to July 2021. Patients were compared according to the period of hospitalization. Primary outcomes were delay times in assistance and clinical outcomes (acute kidney injury [AKI], post-procedural vascular complications and in-hospital mortality). Results: 108 patients were included, 39 (36.1%) in the pre-pandemic period, 13 (12.1%) in pandemic I and 56 (51.8%) in pandemic II. Time from onset of symptoms to arrival at the service and door-to-balloon time did not differ significantly among groups. Vascular complications were more frequent during the pandemic (I and II) than in the pre-pandemic period (2.5% pre-pandemic vs 15.4% pandemic vs 12.5% pandemic II;p = 0.03). AKI incidence was similar in all three periods. There was a non-significant increase in in-hospital mortality during the COVID-19 pandemic. Conclusion: In patients with STEMI, there was an increase in vascular complications and a trend toward increased mortality during the COVID-19 pandemic. Delay times to admission and reperfusion did not differ significantly between before and during the pandemic.

关键词： Myocardial Infarction Primary Percutaneous Coronary Intervention Coronary Reperfusion Pandemic COVID-19

Effects of "Candidatus Liberibacter solanacearum"(haplotype B)on Bactericera cockerelli fitness and vitellogenesis

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Insect Science 2020年第1期27卷 58-68页

作者： Angelica Albuquerque Tomilhero Frias Freddy Ibanez Azucena Mendoza William Mario de carvalho Nunes Cecilia Tamborindeguy Department of Entomology Texas A&M University412 Heep CenterCollege StationTexasUSA DEM-Depto.de Agronomia Nucleo de Pesquisa em Biotecnologia AplicadaMaringaBrazil

"Candidatus Liberibacter solanacearum"(Lso)are phloem-restricted and unculturable Gram-negative *** five haplotypes have been identified worldwide;but only haplotypes A and B are associated with the vector Bactericera cockerelli(Sulc.)in the *** studies showed that Lso-infection reduces *** reproductive output and that Lso haplotype B is more pathogenic than Lso haplotype *** understand the interaction of Lso haplotype B and ***,the fitness of Lso-free and Lso B-infected insects,and the expression of vitellogenin(5cKg7-like),a gene involved directly in the insect reproduction were *** differences in the number of eggs oviposited,and the total number of progeny nymphs and adults were found among crosses of insects with or without *** differences in sex proportions were found between Lso B-infected and Lso-free crosses:a higher proportion of Fi adult females were obtained from Lso B-infected mothers.A significant reduction of BcVgl-like was observed in crosses performed with Lso B-infected females compared to the Lso-free *** female cohorts of different age,a significant reduction of BcVgl-like expression was measured in 7-d-old Lso B-infected females(virgin and mated)compared with 7-d-old Lso-free females(virgin and mated),*** reduction of BcVgl-like transcript was associated with a lower number of developing oocytes observed in female5s reproductive ***,this study represents the first step to understand the interaction of Lso B with ***,highlighting the effect of Lso B infection on egg production,BcVgl-like expression,and oocyte development.

关键词： feminization insect-pathogen interaction oviposition psyllids reproduction vitellogenesis

Effects of climate changes on distribution of Eremanthus erythropappus and ***(Asteraceae)in Brazil

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Journal of Forestry Research 2020年第2期31卷 353-364页

作者： Lucas Fernandes Rocha Isaias Emilio Paulino do Carmo Joema Souza Rodrigues Povoa Dulcineia de carvalho Departamento de Ciencias Florestais Universidade Federal de LavrasLavrasMG CEP 37200-000Brazil Institute of Biosystems Engineering Poznan University of Life SciencesWojska Polskiego 5060-637 PoznanPoland

Phylogeographic patterns of endemic species are critical keys to understand its adaptation to future climate ***,based on chloroplast DNA,we analyzed the genetic diversity of two endemic and endangered tree species from the Brazilian savanna and Atlantic forest(Eremanthus erythropappus and Eremanthus incanus).We also applied the climate-based ecological niche modeling(ENM)to evaluate the impact of the Quaternary climate(last glacial maximum*21 kyr BP(thousand years before present)and Mid-Holocene*6 kyr BP)on the current haplotype ***,we modeled the potential effect of future climate change on the species distribution in 2070 for the most optimistic and pessimistic *** primer/enzyme combination(SFM/HinfI)revealed polymorphism with very low haplotype diversity,showing only three different *** haplotype 1 has very low frequency and it was classified as the oldest,diverging from six mutations from the haplotypes 2 and *** *** populations are structured and differ genetically according to the areas of *** general,the populations located in the north region are genetically different from those located in the *** genetic structuring was observed for *** ENM revealed a large distribution during the past and a severe decrease in geographic distribution of *** and *** from the LGM until present and predicts a drastic decline in suitable areas in the *** reduction may homogenize the genetic diversity and compromise a relevant role of these species on infiltration of groundwater.

关键词： Ecological niche modeling Genetic diversity Climate change Chloroplast DNA

Clinicopathological Description of a Chronic Lymphocytic Leukemia in a Dog

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Open Journal of Veterinary Medicine 2024年第7期14卷 155-162页

作者： Maria Clara Boni Raffi Giovanna Valverde Magalhães Barbosa Alana Carolina Capais Rodrigues Sheila Canevese Rahal John P. Kastelic Luíz Guilherme Dercore Benevenuto Iolanda Simões Braga Beatriz Poloni Batista Felipe César Da Silva Brasileiro Fernanda Barthelson carvalho de Moura Priscylla Tatiana Chalfun Guimarães Okamoto Regina Kiomi Takahira Alessandra Melchert Noeme Sousa Rocha Department of Veterinary Clinics School of Veterinary Medicine and Animal Science So Paulo State UniversityUNESP Botucatu SP Brazil Independent Veterinary Practitioner Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science So Paulo State UniversityUNESP Botucatu SP Brazil Faculty of Veterinary Medicine University of Calgary Calgary Canada

Hematopoietic tumors are associated with diagnostic and therapeutic challenges and a poor prognosis. Furthermore, their pathophysiology in dogs is not well understood. Chronic lymphocytic leukemia has been rarely reported in golden retrievers, perhaps due to difficulties in diagnosis and care. An 8-year-old spayed female golden retriever was referred to our hospital with a 2-month history of fatigue, weakness, and selective appetite. The dog was diagnosed with chronic lymphocytic leukemia based on blood smear evaluation. Despite treatment, the dog deteriorated and the owners opted for euthanasia. Histopathological examination revealed atypical lymphocytes in the spleen, extramedullary hematopoiesis in the spleen and liver, bone marrow depletion, moderate neutrophilic myocarditis, and acute renal tubular necrosis. In an immunohistochemical panel, cells in the spleen and bone marrow were positive for anti-CD20, anti-CD45, anti-CD3, anti-IBA-1, anti-Ki67, anti-MUM-1, and anti-PAX-5. In conclusion, the leukemia diagnosis was confirmed through reactive histiocytosis, marked extramedullary hematopoiesis, and other clinical and laboratory findings. Herein, we report a case of chronic lymphocytic leukemia in a female dog with clinical, laboratory, and necroscopy findings.

关键词： Canine Cancer Neoplasia Therapeutics