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Anti-melanogenic properties of FBCC-EP850 derived from Carex pumila Thunb

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Asian Pacific Journal of Tropical Biomedicine 2024年第11期14卷 477-485页

作者： Mirissa Hewage Dumindu Kavinda Jinkuk Park Nayeong kim Yung Hyun Choi gi-young kim Department of Marine Life Science Jeju National UniversityJeju 63243Republic of Korea Department of Biochemistry College of Korean MedicineDong-Eui UniversityBusan 47227Republic of Korea

Objective:To elucidate the anti-melanogenic potential of Carex pumila ***(FBCC-EP850).Methods:A collection of 180 plant extracts was tested for inhibition of mushroom tyrosinase activity using an in vitro *** them,FBCC-EP850 exhibited the most promising inhibitory *** analysis was conducted to investigate its mechanisms and therapeutic potential in reducing melanogenesis in B16F10 melanoma cells and zebrafish ***:FBCC-EP850 inhibited mushroom tyrosinase activity in a dose-dependent manner,with a half-maximal inhibitory concentration of 45.83μg/***-EP850 at concentrations up to 50μg/mL demonstrated minimal cytotoxicity against B16F10 melanoma cells and no adverse effects on zebrafish *** with 50μg/mL of FBCC-EP850 significantly reducedα-melanocyte stimulating hormone-induced melanin production and suppressed cellular tyrosinase activity in B16F10 melanoma ***,FBCC-EP850 at 25 and 50μg/mL effectively diminished hyperpigmentation inα-melanocyte stimulating hormone-stimulated zebrafish *** anti-melanogenic action could be attributed to modulation of the cAMP-CREB-MITF signaling ***:Carex pumila extract can inhibit melanogenesis by modulating the cAMP-CREB-MITF signaling pathway,which can be used as a promising candidate for treating hyperpigmentation disorders.

关键词： Carex pumila Thunb. Melanogenesis Tyrosinase cAMP-CREB-MITF signaling

Molecular mechanisms of ZD1839 (Iressa)-induced apoptosis in human leukemic U937 cells

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Acta Pharmacologica Sinica 2007年第8期28卷 1205-1214页

作者： Dong-oh MOON Moon-ok kim Jae-dong LEE Yung-hyun CHOI Min-ki LEE gi-young kim Faculty of Applied Marine Science Cheju National UniversityJeju Special Self-Governing Province 690-756Republic of Korea Department of Microbiology Pusan National UniversityBusan 614-052Republic of Korea Department of Biochemistry Dongeui University College of Oriental MedicineBusan 614-052Republic of Korea Department of Internal Medicine Pusan National University College o MedicineBusan 602-739Republic of Korea

Aim:To investigate the molecular mechanisms of ZD1839-induced apoptosis inhuman leukemic U937 ***:The inhibition of human leukemic U937 cellgrowth was assessed by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphnyl-2H-tetrazolimbromide（MTT）assays,lactate dehydrogenase（LDH）release,and cell *** expression of anti- and pro-apoptotic proteins was detected byWestern blot ***:This study demonstrated that ZD 1839 inducedapoptosis in leukemic U937 cells by the downregulation of Bcl-2,caspase activa-tion and subsequent apoptotic *** with ZD 1839 and the caspase-3 inhibitor z-DEVD-fmk blocked apoptosis,indicating that caspase-3 activation isat least partially responsible for ZD1839-induced *** ectopic expres-sion of Bcl-2 attenuated caspase-3 activation,PARP cleavage,and subsequentindicators of apoptosis,including sub-G1DNA content and LDH *** indicate that the downregulation of Bcl-2 plays a major role in the initiationof ZD1839-induced apoptosis,and that the activation of a caspase cascade isinvolved in the execution of ***,ZD 1839 treatment triggeredthe activation of p38 mitogen-activated protein kinase（MAPK）and the down-regulation of c-Jun-N-terminal kinase（JNK）,extracellular signal-regulated kinase（ERK）and phosphatidyl inositol 3-kinase（PI3K）/*** inhibition of the ERKand PI3K/Akt pathways also significantly increased cellular ***:ZD 1839 activated caspase-3 and the inhibited Bcl-2 in human leukemic U937 cellsthrough the downregulation of the ERK and PI3K/Akt pathways.

关键词： ZD1839(Iressa) apoptosis caspase-3 Bcl-2 mitogen-activated protein kinases

Aqueous extract of Protaetia brevitarsis larvae increases mTOR-mediated growth rate in zebrafish larvae

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Asian Pacific Journal of Tropical Biomedicine 2023年第8期13卷 339-347页

作者： Jayasingha Arachchige Chathuranga Chanaka Jayasingha Kyoung Tae Lee Yung Hyun Choi gi-young kim Department of Marine Life Science Jeju National UniversityJeju 63243Republic of Korea Forest Bioresources Department Forest Microbiology DivisionNational Institute of Forest ScienceSuwon 16631Republic of Korea Department of Biochemistry College of Korean MedicineDong-Eui UniversityBusan 47227Republic of Korea

Objective:To evaluate the effects of an aqueous extract of Protaetia brevitarsis(AEPB)on the growth of zebrafish and preosteoblast MC3T3-E1 ***:The effects of AEPB on the linear growth and the expression of growth-related genes in zebrafish and MC3T3-E1 cells were assessed using various molecular ***,the involvement of the mammalian target of rapamycin(mTOR)pathway in AEPB-induced growth was investigated by employing the mTOR inhibitor ***:AEPB administration led to a significant and dose-dependent increase in zebrafish larvae growth over ***,AEPB treatment upregulated the expression of growth hormone-1(GH-1),insulin-like growth factor-1(IGF-1),growth hormone receptor-1(GHR-1),and cholecystokinin-a(CCKA)in ***,AEPB stimulated the expression and release of IGF-1 and accelerated mTOR expression in MC3T3-E1 *** addition,rapamycin hindered AEPB-induced linear growth in zebrafish larvae and suppressed the expression of growth-promoting genes by inhibiting mTOR ***:AEPB shows growth-promoting effects by upregulating growth-related genes and activating the mTOR signaling *** investigations are warranted to elucidate its mechanisms of action and explore its potential application in the development of growth-enhancing supplements for various purposes.

关键词： Protaetia brevitarsis Growth rate Growth hormone Insulin-like growth factor mTOR MC3T3-E1

Hydrangeae Dulcis Folium Attenuates Physical Stress by Supressing ACTH-Induced Cortisol in Zebrafish

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Chinese Journal of Integrative Medicine 2020年第2期26卷 130-137页

作者： Junyoung Oh Dong Hyun kim gi-young kim Eun-Jin Park Jong Hoon Ryu Ji Wook Jung Se Jin Park Gwang-Woo kim Seungheon Lee Department of Marine Life Science College of Ocean ScienceJeju National UniversityJeju(63243)Republic of Korea Departme nt of Medicinal Biotech no logy College of Health SciencesDong-A UniversityBusan(604-714)Republic of Korea Department of Food Bioengineering Jeju National UniversityJeju(63243)Republic of Korea Institute of Convergence BioHealth Dong-A UniversityBusan(604-714)Republic of Korea Department of Oriental Pharmaceutical Science College of PharmacyKyung Hee UniversityHoeki-dongDongdaemoon・KuSeoul(130-701)Republic of Korea Kyung Hee East-West Pharmaceutical Research Institute College of PharmacyKyung Hee UniversityHoeki-dongDongdaemoorvKuSeoul(130-701)Republic of Korea Department of Herbal Medicinal Pharmacology College of Herbal Bio-industryDaegu Haany UniversityYugok-dongGyeongsan(712-715)Republic of Korea School of Natural Resources and Environmental Sciences Kangwon National UniversityChuncheon(24341)Republic of Korea Marine Bio Con verge nee Center Gangneung Science&In dustry Promotion AgencyGangneung(25440)Republic of Korea

Objective:To determine the effects of Hydrangeae Dulcis Folium(EHDF)on physical stress,changes in the whole-body cortisol level and behaviour in zebrafish(Danio rerio).Methods:One hundred and seventy-four fish were randomly divided into 4[adrenocorticotropin hormone(ACTH)challenge test:4 fish per group]or 6 groups(behavioural test:10–12 fish per group,whole-body cortisol:4 fish per group).Net handling stress(NHS)was used to induce physical *** were treated with vehicle or EHDF(5–20 mg/L)for 6 min before they were exposed to *** then,fish were sacrificed for collecting body fluid from whole-body or conducted behavioural tests,including novel tank test and open field test,and were evaluated to observe anxiety-like behaviours and *** addition,to elucidate the mode of action of the anti-stress effects of EHDF,ACTH(0.2 IU/g,i.p.)challenge test was ***:The increased anxiety-like behaviours in novel tank test and open field test under stress were prevented by treatment with EHDF at 5–20 mg/L(P<0.05).Moreover,compared with the unstressed group,which was not treated with NHS,the whole-body cortisol level was significantly increased by treatment with NHS(P<0.05).Compared with the NHS-treated stressed control group,pre-treatment with EHDF at concentrations of 5–20 mg/L for 6 min significantly prevented the NHS-increased whole-body cortisol level(P<0.05).In addition,ACTH challenge test showed that EHDF completely blocked the effects of ACTH on cortisol secretion(P<0.05).Conclusion:EHDF may be a good antistress candidate and its mechanism of action may be related to its positive effects on cortisol release.

关键词： net handling stress zebrafish Hydrangeae Dulcis Folium whole-body cortisol behavioural tests

Ethanol extract of Kalopanax septemlobus leaf inhibits HepG2 human hepatocellular carcinoma cell proliferation via inducing cell cycle arrest at G_1 phase

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Asian Pacific Journal of Tropical Medicine 2016年第4期9卷 336-342页

作者： Cheol Park Ji-Suk Jeong Jin-Woo Jeong Sung Ok kim Yong-Joo kim gi-young kim Su-Hyun Hong Yung Hyun Choi Department of Molecular Biology College of Natural Sciences and Human EcologyDongeui University Department of Research and Development of Resources Gurye-gun Agricultural Center Wild Flower Institute.Gurye-gun Anti-Aging Research Center.Dongeui University Department of Food Science & Biotechnology College of EngineeringKyungsung University Republic of Korea Laboratory of Immunobiology.Department of Marine Life.Sciences.Jeju National University Department of Biochemistry.Dongeui University

Objective:To investigate the effects of an ethanol extract of Kalopanax septemlobus(Thunb.)***(EEKS) on cell proliferation in human hepatocellular carcinoma cells and its mechanisms of ***:Cells were treated with EEKS and subsequently analyzed for cell proliferation and flow cytometry *** of cell cycle regulators were determined by reverse transcriptase polymerase chain reaction analysis and Western blotting,and activation of eyclin-associaled kinases studied using kinase ***:The EEKS suppressed cell proliferation in both HepG2 and Hep3 B cells,but showed a more sensitive anli-proliferative activity in HepG2 *** cytometry analysis revealed an association between the growth inhibitory effect of EEKS and with G_1 phase cell cycle arrest in HepG2 cells,along with the dephosphorylation of retinoblastoma protein(pRB) and enhanced binding of pRB with the E2 F transcription factor family *** with EEKS also increased the expression of cyclin-dependent kinase(CDK) inhibitors,such as p21WAF1/CIP1 and *** any noticeable changes in G_1 cyclins and CDKs(except for a slight decrease in CDK4).Treatment of HepG2 cells with EEKS also increased the binding of p21 and p27 with CDK4 and *** was paralleled by a marked decrease in the cyclin D- and cyclin E-associated kinase ***:Overall,our findings suggest that EEKS may be an effective treatment for liver cancer through suppression of cancer cell proliferation via G_1,cell cycle arrest Further studies arc required to identify the active compounds in EEKS.

关键词： Kalopanax septemlobus Hepatocellular carcinoma G1 cell cycle arrest CDK inhibitor pRB

Methanol extract of Codium fragile inhibits tumor necrosis factor-ɑ-induced matrix metalloproteinase-9 and invasiveness of MDA-MB-231 cells by suppressing nuclear factor-κB activation

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Asian Pacific Journal of Tropical Medicine 2016年第6期9卷 520-525页

作者： Matharage Gayani Dilshara Rajapaksha Gedara Prasad Tharanga Jayasooriya Chang-Hee Kang Yung-Hyun Choi gi-young kim Department of Marine Life Sciences Jeju National University Nakdonggang National Institute of Biological Resource Department of Biochemistry College of Oriental Medicine Dong-Eui University

Objective:To evaluate whether the methanol extract of Codium fragile(MECF) regulates tumor necrosis factor-α(TNF-α)-induced invasion of human breast cancer MDA-MB-231 cells by suppressing matrix metalloproteinase-9(MMP-9).Methods:Reverse transcriptionpolymerase chain reaction(RT-PCR) and western blot analysis were performed to analyze the expression of MMP-9 and nuclear factor-κB(NF-κB) subunits,p65 and p50,and IκB in MDA-MB-231 cells.3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) assay was used for cell ***-9 activity and invasion were measured by gelatin zymography and a matrigel invasion assay,***- κB activity was measured by an electrophoretic mobility shift assay and luciferase ***:MECF had no effects on cell viability up to a concentration of 100 μg/mL in human breast cancer MDA-MB-231 cells regardless of the presence of TNF-α.MDA-MB-231 cells that were stimulated with TNF-α showed a marked increase of invasion compared to the untreated control,whereas pretreatment with MECF downregulated the TNF-α-induced invasion of MDA-MB-231 ***,zymography,western blot analysis,and reverse transcriptase-polymerase chain reaction(RT-PCR) confirmed that MECF decreased TNF-α-induced MMP-9 expression and activity which is a key regulator for cancer *** to an electrophoretic morbidity shift assay,pretreatment with MECF in MDA-MB-231 cells significantly decreased the TNF-α-induced DNA-binding activity of nuclear factor- κB(NF- κB),which is an important transcription factor for regulating cancer invasion-related genes such as ***,treatment with MECF sustained the expression of p65 and p50 in response to TNF-α in the cytosolic *** luciferase assay demonstrated that MECF attenuated TNF-α-induced NF- κB luciferase ***:MECF exhibited its antiinvasive capability by downregulating TNF-α-induced MMP-9 expression,resulting from the suppression of NF- κB activi

关键词： Codium fragile Invasion Nuclear factor-κB Matrix metalloproteinase-9 Tumor necrosis factor-α

Capsosiphon fulvescens suppresses LPS-stimulated inflammatory responses by suppressing TLR4/NF-κB activation in RAW264.7 murine macrophages

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Asian Pacific Journal of Tropical Biomedicine 2024年第3期14卷 115-126页

作者： Seon Yeong Ji EunJin Bang Hyun Hwangbo Min Yeong kim Da Hye kim Su Hyun Hong Shin-Hyung Park Chang-young Kwon gi-young kim You-Jin Jeon Suengmok Cho Yung Hyun Choi Department of Biochemistry Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Basic Research Laboratory for the Regulation of Microplastic-Mediated Diseases and Anti-Aging Research Center Dong-eui UniversityBusan 47227Republic of Korea Department of Pathology Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Department of Oriental Neuropsychiatry Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Department of Marine Life Science Jeju National UniversityJeju 63243Republic of Korea Department of Food Science and Technology/Institute of Food Science Pukyong National UniversityBusan 48513Republic of Korea

Objective:To evaluate the effects of Capsosiphon fulvescens(***)ethanolic extract on inflammation in lipopolysaccharide(LPS)-induced RAW296.7 ***:The protective effects of *** ethanolic extract on LPS-induced inflammation in RAW264.7 macrophages were assessed using biochemical analysis,including enzyme-linked immunosorbent assay,quantitative reverse transcription-polymerase chain reaction,and Western blot *** examine reactive oxygen species(ROS)production,flow cytometry analysis,and immunofluorescence staining were ***,the modulatory effect of *** ethanolic extract on NF-κB activation was ***:*** ethanolic extract significantly attenuated LPS-induced levels of pro-inflammatory cytokines and notably reduced the secretion and mRNA levels of LPS-mediated matrix *** addition,*** ethanolic extract decreased ROS production and suppressed the TLR4/NF-κB signaling ***:*** ethanolic extract alleviates inflammation as well as oxidative stress by modulating the TLR4/NF-κB signaling in LPS-induced RAW264.7 *** can be used as a potential therapeutic agent to suppress inflammation and oxidative stress-associated diseases.

关键词： Capsosiphon fulvescens Inflammation Oxidative stress NF-κB Nrf2 TLR4

Ethanol extracts of Hizikia fusiforme induce apoptosis in human prostate cancer PC3 cells via modulating a ROS-dependent pathway

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Asian Pacific Journal of Tropical Biomedicine 2020年第2期10卷 78-86页

作者： Eun Ok Choi Hyesook Lee Cheol Park gi-young kim Hee-Jae Cha Suhkmann kim Heui-Soo kim You-Jin Jeon Hye Jin Hwang Yung Hyun Choi Anti-Aging Research Center Dong-eui UniversityBusan 47340Republic of Korea Department of Biochemistry College of Korean MedicineDong-eui UniversityBusan 47227Republic of Korea Department of Molecular Biology College of Natural SciencesDong-eui UniversityBusan 47340Republic of Korea Laboratory of Immunobiology Department of Marine Life SciencesJeju National UniversityJeju 63243Republic of Korea Department of Parasitology and Genetics Kosin University College of MedicineBusan 49267Republic of Korea Department of Chemistry College of Natural SciencesCenter for Proteome Biophysics and Chemistry Institute for Functional MaterialsPusan National UniversityBusan 46241Republic of Korea Department of Biological Sciences College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Department of Food and Nutrition College of NursingHealthcare Sciences&Human EcologyDong-eui UniversityBusan 47340Republic of Korea

Objective:To investigate whether ethanol extracts of Hizikia fusiforme could induce apoptosis in human prostate cancer PC3 ***:Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium *** and mitochondrial membrane potential(MMP)were measured using flow cytometry in PC3 *** damage was assessed by nuclear staining and DNA fragmentation *** of apoptosis-associated proteins were determined by Western blotting *** of caspase-3,-8,and-9 were determined by colorimetric ***,intracellular reactive oxygen species(ROS)generation was detected using a flow cytometer and fluorescence ***:Treatment of PC3 cells with ethanol extracts of Hizikia fusiforme inhibited proliferation,which was associated with induction of apoptosis,and accompanied by increased expression of Fas,Fas-ligand(Fas L),Bax and t Bid,and decreased expression of *** addition,ethanol extracts of Hizikia fusiforme reduced c-Flip expression and activated caspase-8,-9 and-3,resulting in an increase in poly(ADP-ribose)polymerase(PARP)***,in the presence of a pan-caspase inhibitor,ethanol extracts of Hizikia fusiforme-mediated growth inhibition and apoptosis were significantly *** extracts of Hizikia fusiforme also destroyed the integrity of mitochondria due to the loss of MMP,leading to cytosolic release of cytochrome ***,the levels of ROS were markedly increased by treatment with ethanol extracts of Hizikia fusiforme,which was significantly suppressed by the ROS scavenger *** investigation of whether ethanol extracts of Hizikia fusiforme-induced apoptosis was related to the generation of ROS was conducted and the results showed that N-acetyl-L-cysteine fully blocked ethanol extracts of Hizikia fusiforme-induced apoptotic events including loss of MMP,activation of caspase-3,the cytosolic release of cytochrome c and ***:E

关键词： Hizikia fusiforme Apoptosis Prostate cancer cells Caspase Reactive oxygen species

Ethanol extract of Chondracanthus tenellus(Harvey)Hommersand attenuates lipopolysaccharide-induced inflammatory and oxidative response by blocking the NF-κB,MAPKs,and PI3K/Akt signaling pathways

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Asian Pacific Journal of Tropical Biomedicine 2021年第10期11卷 450-459页

作者： Da Hye Kwon Cheol Park Hyesook Lee Su Hyun Hong gi-young kim Hee-Jae Cha Suhkmann kim Heui-Soo kim Hye-Jin Hwang Yung Hyun Choi Anti-Aging Research Center Dong-eui UniversityBusan 47340Republic of Korea Department of Biochemistry College of Korean MedicineDong-eui UniversityBusan 47227Republic of Korea Division of Basic Sciences College of Liberal StudiesDong-eui UniversityBusan 47340Republic of Korea Department of Marine Life Sciences School of Marine Biomedical SciencesJeju National UniversityJeju 63243Republic of Korea Department of Parasitology and Genetics College of MedicineKosin UniversityBusan 49104Republic of Korea Department of Chemistry College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Department of Biological Sciences College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Department of Food and Nutrition College of NursingHealthcare Sciences&Human EcologyDong-eui UniversityBusan 47340Republic of Korea

Objective:To investigate whether the ethanol extract of Chondracanthus tenellus(Harvey)Hommersand,a type of red algae,could exhibit anti-inflammatory potential in lipopolysaccharide(LPS)-stimulated ***:The ethanol extract of Chondracanthus tenellus was applied to 100 ng/mL LPS-stimulated RAW 264.7 cells,and cell viability,phagocytic ability,levels of pro-inflammatory factors,and the production of reactive oxygen species were *** identify the underlying mechanism of the ethanol extract of Chondracanthus tenellus,the expression of inflammation-regulated genes was ***:The ethanol extract of Chondracanthus tenellus had no cytotoxic effect at concentrations below 300μg/mL,and reduced the LPS-induced production of inflammatory mediators including nitric oxide(NO)and prostaglandin ***,the extract markedly suppressed the expression of inducible NO synthase and cyclooxygenase-2,as well as the production of reactive oxygen *** LPS-induced up-regulation of pro-inflammatory cytokines was attenuated by treatment with the ethanol extract of Chondracanthus tenellus,reducing their extracellular *** Chondracanthus tenellus extract also inhibited LPS-mediated activation of nuclear factor-kappa B(NF-κB).In addition,the phosphorylation of mitogen activated protein kinases(MAPKs)and phosphatidylinositol 3 kinase(PI3K)/Akt was markedly increased by LPS,which was significantly abolished by the Chondracanthus tenellus ***:Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-κB,MAPKs,and PI3K/Akt signaling pathways in LPS stimulated RAW 264.7 *** ***:Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-κB,MAPKs,and PI3K/Akt signaling pathways in LPS stimu

关键词： Chondracanthus tenellus Inflammation ROS NF-κB RAW 264.7 cells