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Pedosphere 2009年第6期19卷 790-798页

作者： LIN Jin-Shi SHI Xue-Zheng LU Xi-Xi YU Dong-Sheng wang Hong-Jie ZHAO Yong-Cun SUN Wei-Xia State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 2100(78 (China) Department of Geography National University of Singapore 119260 (Singapore) Graduate university of the Chinese Academy of sciences Beijing 100049 (China) college of Resources and Environment Fujian Agriculture and Forestry University Fuzhou 350002 (China)

Due to the growing concern about the agricultural phosphorus (P) losses pollution, an in-depth understanding of P in paddy soils of china would be helpful in providing a national perspective of the environmental impact of P cycling and fertility on china's farms. In this study, we evaluated the P storage and the P density of paddy soils in china, characterized the spatial variations of P among the subgroups of paddy soils and soil regions in china, and evaluated the P data using GIS-based analysis, which included a newly compiled 1:1 000 000 digital soil map of china, and using 1 490 soil profiles. The available and total P densities of paddy soils were 6.7 and 698.5 g m -3 , respectively. Overall in china, the total P storage within 1 m of paddy soils was estimated to be 330.2 Tg. The P density of paddy soils varied substantially with subgroups due to the different soil water regimes such as groundwater table and soil drainage. The P availability in paddy soils, especially in surface layer, was higher in high temperature and precipitation areas. Further research is needed to examine more anthropogenic impact factors, such as increasing use of chemical fertilizer.

关键词： paddy soil database paddy soil subgroups phosphorus density phosphorus pool

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Silica nanospheres formation induced by peroxidase-catalyzed phenol polymerization

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Progress in Natural science:Materials International 2003年第7期 23-26页

作者： FANG Jiangyu, wang He, CHEN yang and ZHANG Fusuo (1. college of Resources and Environmental sciences, china agricultural university, beijing 100094, china 2. college of Agronomy, Gansu agricultural university, Lanzhou 730070, china) college of Resources and Environmental sciences，china agricultural university，beijing 100094，china college of Agronomy，Gansu agricultural university，Lanzhou 730070，china college of Resources and Environmental sciences，china agricultural university，beijing 100094，china college of Agronomy，Gansu agricultural university，Lanzhou 730070，china

To examine whether lignin-like compound is correlated with silica precipitation in grass, a series of simulated chemical experiments were carried out at ambient temperature and pressure, close to cell wall pH, with phenol polymerization catalyzed by peroxi-dase in silicon solution. The experiments showed that phenol polymer (a kind of lignin-like substance) caused silica nanosphere precipitation similar to those caused by protein in diatom cell wall previously reported by other authors. The sphere diameter varied with different kinds of phenol and the concentrations of phenol and silicon. Silicon precipitation had phenol and silicon saturation effect, meaning that when the concentration ratio of soluble silicon to phenol exceeded a certain value, the amount of silicon precipitation would decrease.

关键词： silicon phenol peroxidase.

The exact theory of plane problems for piezoelectric media

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The exact theory of plane problems for piezoelectric media

Genetic variation in a heat shock transcription factor modulates cold tolerance in maize

2011年全国压电和声波理论及器件应用研讨会

作者： yang gao~(1,*) Bao-Sheng ZHAO~2 1 college of science,china agricultural university,P.O.Box 74,beijing 100083 2 School of Mechanical Engineering,university of science and Technology Liaoning,Anshan 114044

＜正＞Without employing ad hoc assumptions,various equations and solutions for plane problems of piezoelectric media are deduced systematically.A method for the exact solution of three-dimensional equations is present... 详细信息

关键词： plane problems plate theory piezoelectric the exact theory

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Molecular Plant 2024年第9期17卷 1423-1438页

作者： Lei gao Lingling Pan Yiting Shi Rong Zeng Minze Li Zhuoyang Li Xuan Zhang Xiaoming Zhao Xinru Gong Wei Huang Xiaohong yang Jinsheng Lai Jianru Zuo Zhizhong Gong Xiqing wang Weiwei Jin Zhaobin Dong Shuhua yang State Key Laboratory of Plant Environmental Resilience Frontiers Science Center for Molecular Design BreedingCollege of Biological SciencesCenter for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBeijing 100193China State Key Laboratory of Maize Bio-breeding National Maize Improvement CenterFrontiers Science Center for Molecular Design BreedingDepartment of Plant Geneticsand BreedingChinaAgricultural UniversityBeijingChina State Key Laboratory of Plant Genomics and National Plant Gene Research Center Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing100101China

Understanding how maize(Zea mays)responds to cold stress is crucial for facilitating breeding programs of cold-tolerant *** extensive utilization of the genome-wide association study(GWAs)approach for exploring favorable natural alleles associated with maize cold tolerance,few studies have successfully identified candidate genes that contribute to maize cold *** this study,we used a diverse panel of inbred maize lines collected from different germplasm sources to perform a GWAS on var-iations in the relative injured area of maize true leaves during cold stress-a trait very closely correlated with maize cold *** identified HsF21,which encodes a B-class heat shock transcription factor(HSF)that positively regulates cold tolerance at both the seedling and germination *** varia-tions in the promoter of the cold-tolerant HSF21Hap1 allele led to increased HSF21 expression under cold stress by inhibiting binding of the basic leucine zipper bziP68 transcription factor,a negative regulator of cold *** integrating transcriptome deep sequencing,DNA affinity purification sequencing,and targeted lipidomic analysis,we revealed the function of HsF21 in regulating lipid metabolism homeo-stasis to modulate cold tolerance in *** addition,we found that HsF21 confers maize cold tolerance without incurring yield ***,this study establishes HsF21 as a key regulator that en-hances cold tolerance in maize,providing valuable genetic resources for breeding of cold-tolerant maize varieties.

关键词： heat shock factor HSF21 cold tolerance natural variation bZiP68 lipid metabolism maize

Single nucleotide polymorphisms in chicken lmbr1 gene were associated with chicken growth and carcass traits

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science china(Life sciences) 2007年第1期50卷 62-69页

作者： HUANG YanQun1,2,3, DU XiaoHui2, DENG XueMei4, QIU XiangPin2, wang ChunKao4, CHEN Wen3, LI Ning1 & WU ChangXin4 1 State Key Laboratory for Agrobiotechnology, china agricultural university, beijing 100094, china 2 college of Animal science, Sichuan agricultural university, Ya’an 625014, china 3 college of Animal Husbandry and Veterinary, Henan agricultural university, Zhengzhou 450002, china 4 college of Animal science and Technology, china agricultural university, beijing 100094, china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of Animal science Sichuan Agricultural University Ya'an 625014 China college of Animal Husbandry and Veterinary Henan Agricultural University Zhengzhou 450002 China college of Animal science Sichuan Agricultural University Ya'an 625014 China college of Animal science and Technology China Agricultural University Beijing 100094 China college of Animal Husbandry and Veterinary Henan Agricultural University Zhengzhou 450002 China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China

Lmbr1 is the key candidate gene controlling vertebrate limb development, but its effects on animal growth and carcass traits have never been reported. In this experiment, lmbr1 was taken as the candi-date gene affecting chicken growth and carcass traits. T/C and G/A mutations located in exon 16 and one A/C mutation located in intron 5 of chicken lmbr1 were detected from Silky, White Plymouth Rock broilers and their F2 crossing chickens by PCR-SSCP and sequencing methods. The analysis of vari-ance (ANOVA) results suggests that T/C polymorphism of exon 16 had significant association with eviscerated yield rate (EYR), gizzard rate (GR), shank and claw rate (SCR) and shank girth (SG); A/C polymorphism of intron 5 was significantly associated with SCR, liver rate and head-neck weight (HNW), while both sites had no significant association with other growth and carcass traits. These results demonstrate that lmbr1 gene could be a genetic locus or linked to a major gene significantly affecting these growth and carcass traits in chicken.

关键词： chicken lmbr1 SNPs shank and claw rate carcass traits

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Pathogen-induced methylglyoxal negatively regulates rice bacterial blight resistance by inhibiting OsCDR1 protease activity

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Molecular Plant 2024年第2期17卷 325-341页

作者： Zheng-Wei Fu Jian-Hui Li Xiang gao Shi-Jia wang Ting-Ting Yuan Ying-Tang Lu State Key Laboratory of Hybrid Rice College of Life SciencesRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430072China Key Laboratory of Biology and Genetic Improvement of OilCrops Ministry of Agriculture and Rural AffairsOilCrops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062China

Xanthomonas oryzae ***(Xoo)causes bacterial blight(BB),a globally devastating disease of rice(Oryza sativa)that is responsible for significant crop *** and sugar metabolites are important for pathogen infection,providing energy and regulating events associated with defense responses;howev-er,the mechanisms by which they regulate such events in BB are *** an inevitable sugar metabolite,methylglyoxal(MG)is involved in plant growth and responses to various abiotic stresses,but the underlying mechanisms remain *** and how MG functions in plant biotic stress responses is almost completely ***,we report that the Xoo strain PXO99 induces OsWRKY62.1 to repress transcrip-tion of OsGLY Il genes by directly binding to their promoters,resulting in overaccumulation of *** negatively regulates rice resistance against Pxo99:osglyll2 mutants with higher MG levels are more sus-ceptible to the pathogen,whereas OsGLYIl2-overexpressing plants with lower MG content show greater resistance than the wild *** of OsGLYll2 to prevent excessive MG accumulation confers broad-spectrum resistance against the biotrophic bacterial pathogens Xoo and Xanthomonas oryzae *** and the necrotrophic fungal pathogen Rhizoctonia solani,which causes rice sheath *** evidence shows that MG reduces rice resistance against PXO99 through CONSTITUTIVE DISEASE RESISTANCE 1(OsCDR1).MGmodifies the Arg97 residue of OsCDR1to inhibit its aspartic protease activ-ity,which is essential for OsCDR1-enhanced *** together,these findings illustrate how Xoo promotes infection by hijacking a sugar metabolite in the host plant.

关键词： Oryza sativa bacterial blight OsWRKY62.1 methylglyoxal OsCDR1

Mapping QTLs on BTA6 affecting milk production traits in a Chinese Holstein population

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science Bulletin 2005年第16期 67-72页

作者： CHEN Huiyong1, ZHANG Qin2, wang Chunkao1, SHU Juan1, MEI Gui1, YIN Cengceng1, HU Fang1, XU Jingjing1, GONG Weijia1, LI Hejun1 & QIU Xiaotian1 1. Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, college of Animal science and Technology, china Agri-cultural university, beijing 100094, china 2. college of Animal science and Technology, china agricultural Uni-versity, beijing 100094, china

A Chinese Holstein population with daughter design was analyzed using 14 microsatellites covering a map distance of 55.7 cM on chromosome 6 to fine map QTL for five milk production traits. 26 paternal half-sib families with 2356 daughters were involved. Two different approaches, linear regression approach and variance component ap-proach, were employed, with a one-QTL model and two-QTL model fitted. With a one-QTL model, the linear regression approach revealed a QTL near BMS470 with effects on milk yield, fat yield, protein yield, and fat percentage, and another QTL near BMS2460 for protein percentage. The variance component approach confirmed the results of linear regres-sion approach for the three yield traits, with the exception that the QTL for fat yield was mapped to a different position near BMS1242. The 95% confidence intervals resulted from linear regression, obtained by bootstrapping, were generally large, ranging from 31 to 53 cM, whereas the variance com-ponent approach revealed very small confidence intervals, calculated by LOD drop-off method, for the three yield traits, only 4―5 cM. With a two-QTL model, both approaches pro-vided strong evidence for the existence of two QTLs for the three yield traits. Along with the QTLs identified in one-QTL model analyses, the linear regression approach revealed a second QTL near BP7 with effects on all the three yield traits, whereas the variance component approach located the sec-ond QTL near ILSS035, BMS470, and BP7 for the three traits, respectively.

关键词： Chinese Holstein cattle daughter design milk produc-tion trait linear regression approach variance component approach.

Efficient inhibition of SARS-CoV-2 emerging EG.5,EG.5.1 and BA.2.86 variants by fusion inhibitor HY3000 peptide

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hLife 2024年第1期2卷 43-46页

作者： Lili Wu Anqi Zheng yangming Tang Xiaoyun wang Yue gao Wenwen Lei Guizhen Wu Qihui wang George Fu gao CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina university of the Chinese Academy of sciences BeijingChina Hybio Pharmaceutical Co. Ltd.GuangdongChina college of Life science Hebei UniversityHebeiChina NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Chinese Center for Disease Control and Prevention BeijingChina

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)continues to pose a significant threat to the world,as it continually evolves and gives rise to multiple variants and ***,the Omicron EG.5 linage,which was first detected in Indonesia on 17 February 2023,has raised concerns due to its increased prevalence and extended immune escape properties,according to the risk analysis by the World Health Organization(WHO)[1].As of 3 October 2023,EG.5 and its sub-linages have been reported in 83 countries with shared 49,008 genome sequences in GISAID database(https://***/hcov19-variants/).EG.5 has become the dominant strain in the United States according to Centers for Disease Control and Prevention(CDC),accounting for 29.4%of SARS-CoV-2 infections(https://***/covid-data-tracker/#variant-proportions).

关键词： acute respiratory variants

SARS-CoV-2 ORF10 hijacking ubiquitination machinery reveals potential unique drug targeting sites

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Acta Pharmaceutica Sinica B 2024年第9期14卷 4164-4173页

作者： Kaixiang Zhu Lili Song Linyue wang Lei Hua Ziyu Luo Tongyun wang Bo Qin Shuofeng Yuan Xiaopan gao Wenyi Mi Sheng Cui NHC Key Laboratory of Systems Biology of Pathogens National Institute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Key Laboratory of Pathogen Infection Prevention and Control(Peking Union Medical college) Ministry of EducationBeijing 100730China The Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics Key Laboratory of Immune Microenvironment and Disease(Ministry of Education)Tianjin Medical University General HospitalDepartment of ImmunologyTianjin Medical UniversityTianjin 300070China Medical School Yan’an UniversityShaanxi ProvinceYan’an 716000China State Key Laboratory of Emerging Infectious Diseases Li Ka Shing Faculty of Medicinethe University of Hong KongPokfulamHong Kong SARChina Department of Microbiology Li Ka ShingFaculty of Medicinethe University of Hong KongPokfulamHong Kong SARChina

Viruses often manipulate ubiquitination pathways to facilitate their replication and ***2ZYG11B known as the substrate receptor of cullin-2 RING E3 ligase,is bound by SARS-CoV-2 ORF10 to increase its E3 ligase activity,leading to degradation of IFT46,a protein component of the intraflagellar transport(IFT)complex *** results in dysfunctional cilia,which explains certain symptoms that are specific to ***,the precise molecular mechanism of how ORF10 recognizes CUL2ZYG11B remains ***,we determined the crystal structure of CUL2ZYG11B complexed with the N-terminal extension(NTE)of SARS-CoV-2 ORF10(2.9 A°).The structure reveals that the ORF10 N-terminal heptapeptide(NTH)mimics the Gly/N-degron to bind *** studies identified key residues within ORF10 that are key players in its interaction with CUL2ZYG11B both in ITC assay and in vivo *** addition,we prove that enhancement of CUL2ZYG11B activity for IFT46 degradation by which ORF10-mediated correlates with the binding affinity between ORF10 and ***,we used a Global Protein Stability system to show that the NTH of ORF10 mimics the Gly/N-degron motif,thereby binding competitively to CUL2ZYG11B and inhibiting the degradation of target substrates bearing the Gly/N-degron ***,this study sheds light on how SARS-CoV-2 ORF10 exploits the ubiquitination machinery for proteasomal degradation,and offers valuable insights for optimizing PROTAC-based drug design based on NTH CUL2ZYG11B interaction,while pinpointing a promising target for the development of treatments for COVID-19.

关键词： SARS-CoV-2 ORF10 Crystal structure cullin-2 RING E3 ligase Drug design