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Transcriptome analysis reveals effects of red and blue lightemitting diodes(LEDs)on the growth,chlorophyll fluorescence and endogenous plant hormones of potato(Solanum tuberosum L.)plantlets cultured in vitro

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Journal of Integrative Agriculture 2021年第11期20卷 2914-2931页

作者： CHEN Li-li WANG Hao-ying GONG Xiao-chen ZENG zhao-hai xuE xu-zhang HU Yue-gao college of science&Technology Ningbo UniversityCixi 315300P.R.China college of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193P.R.China beijing Research Center for Intelligent Equipment in Agriculture Beijing Academy of Agriculture and Forestry SciencesBeijing 100097P.R.China

Red and blue light illumination has been reported to significantly affect plantlet *** is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ***,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome *** objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq *** total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,*** plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under *** dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato *** addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato *** results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro

关键词： potato(Solanum tuberosum L.)plantlets in vitro red/blue LEDs light sources RNA-seq chlorophyll fluorescence plant hormone

Planting uniformity performance of motor-driven maize precision seeding systems

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International Journal of agricultural and Biological Engineering 2022年第5期15卷 101-108页

作者： Shuo yang Changyuan Zhai Yuanyuan gao Hanjie Dou xueguan zhao Yakai He Xiu Wang Research Center of Information Technology Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Intelligent Equipment Research Center Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China National Engineering Research Center for Information Technology in Agriculture Beijing 100097China School of agricultural Equipment Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina

Low accuracy planting uniformity affects *** meter type and forward speed typically interfere with the planting uniformity accuracy of motor-driven seeding *** types of maize precision planters equipped with motor-driven planting systems are investigated in this study to ascertain the rule of planting uniformity in both simulated and field *** simulated speed increases from 5 to 12 km/h at a 1 km/h interval in a laboratory *** test results show that the quality of feed index(QTFI)of the two planters decreased by 16.79%and 9.88%.This is primarily attributed to the increase in the miss index(MISS)by 11.62%and 9.70%,*** field speed was set to four levels from 5 to 12 km/h in a field *** plant spacing scatter distribution results were analyzed,and the results of the two planters indicated that the average positive difference of the two planters linearly increased with the forward speed,and the negative difference of the two planters did not exhibit a linear *** number of positive moving average points was 2.49 times greater than that of the negative moving average points of the finger pick-up maize precision planter,and 4.49 times in the air-suction maize precision *** results indicated that the increase of the positive difference of plant spacing is the major effect factor in the field planting uniformity of the two motor-driven maize precision *** addition,the plant spacing corresponded to the distribution frequency of the two planters in field was close to the target seed spacing of 25 cm with a max coefficient of variation(CV)of 21.55%and 20.66%,respectively,and those plant spacing values corresponded to max distribution frequency of the two planters at the four level field speeds were(24.69±0.63)cm and(25.63±0.32)cm,***,the multiples index(MUL)changed randomly affected by the increasing *** research results provide a direction for the optimization design of

关键词： electric drive metering planting uniformity maize precision planting quality evaluation

CmNF-YB8 affects drought resistance in chrysanthemum by altering stomatal status and leaf cuticle thickness

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Journal of Integrative Plant Biology 2022年第3期64卷 741-755页

作者： Tianle Wang Qian Wei Zhiling Wang Wenwen Liu Xin zhao Chao Ma Junping gao Yanjie xu Bo Hong State Key Laboratory of Agrobiotechnology Beijing Key Laboratory of Development and Quality Control of Ornamental CropsDepartment of Ornamental HorticultureCollege of HorticultureChina Agricultural UniversityBeijing100193 China Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm College of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou510642 China

Drought is a major abiotic stress that limits plant growth and *** mechanisms have evolved to mitigate drought stress,including the capacity to adjust water loss rate and to modify the morphology and structure of the ***,we show that the expression of CmNF-YB8,encoding a nuclear factor Y(NF-Y)B-type subunit,is lower under drought conditions in chrysanthemum(Chrysanthemum morifolium).Transgenicchrysanthemumlinesinwhich transcript levels of CmNF-YB8 were reduced by RNA interference(CmNF-YB8-RNAi)exhibited enhanced drought resistance relative to control lines,whereas lines overexpressing CmNF-YB8(CmNF-YB8-OX)were less tolerant to *** to wild type(WT),CmNF-YB8-RNAi plants showed reduced stomatal opening and a thicker epidermal cuticle that correlated with their water loss *** also identified genes involved in stomatal adjustment(CBLinteracting protein kinase 6,CmCIPK6)and cuticle biosynthesis(CmSHN3)that are more highly expressed in CmNF-YB8-RNAi lines than in WT,CmCIPK6 being a direct downstream target of ***-induced gene silencing of CmCIPK6 or CmSHN3 in the CmNFYB8-RNAi background abolished the effects of CmNF-YB8-RNAi on stomatal closure and cuticle deposition,***-YB8 thus regulates CmCIPK6 and CmSHN3 expression to alter stomatal movement and cuticle thickness in the leaf epidermis,thereby affecting drought resistance.

关键词： Chrysanthemum morifolium cuticle drought stress NF-YB8 stomatal status

应用近红外光谱分析不同产地雪茄烟叶的相似性

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光谱学与光谱分析 2024年第11期44卷 3195-3198页

作者：赵高坤李嘉辰吴玉萍李军会孔光辉张光海姚恒李薇高艳兰云南烟草农业科学研究院中国农业大学信息与电气工程学院

雪茄风味特征与产地密切相关。应用具有快速、无损特点的近红外光谱技术分析不同产地雪茄烟叶相似性，为国内雪茄种植区划和产品设计提供依据。以2021年—2023年在云南以及巴西、多米尼加、印尼收集的526个烟叶样品，使用基于主成分与Fi... 详细信息

雪茄风味特征与产地密切相关。应用具有快速、无损特点的近红外光谱技术分析不同产地雪茄烟叶相似性，为国内雪茄种植区划和产品设计提供依据。以2021年—2023年在云南以及巴西、多米尼加、印尼收集的526个烟叶样品，使用基于主成分与Fisher准则相似性方法分析不同产地烟叶NIR相似性。结果表明：巴西与多米尼加烟叶相似性高，而云南与多米尼加烟叶具有一定相似性；云南区域内，玉溪、文山、普洱之间烟叶相似性高，德宏与临沧之间相似性较高；德宏和多米尼加之间具有一定的相似性，临沧与印尼之间具有一定的相似性。大产区范围内得到的相似性结果与感官评价具有一致性，更细化的相似性分析结果可为国产雪茄烟叶种植区划、产品设计等提供更详尽的技术支撑，例如优质雪茄烟叶的产地替换及互补配伍等。

关键词：雪茄烟叶产地近红外光谱相似性分析

Characterization of wheat monogenic lines with known Sr genes and wheat cultivars for resistance to three new races of Puccinia graminis f. sp. tritici in china

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Journal of Integrative Agriculture 2023年第6期22卷 1740-1749页

作者： WU Xian-xin ZANG Chao-qun ZHANG Ya-zhao xu Yi-wei WANG Shu LI Tian-ya gao Li State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China college of Plant Protection Shenyang Agricultural UniversityShenyang 110866P.R.China Institute of Plant Protection Liaoning Academy of Agricultural SciencesShenyang 110161P.R.China Agronomy college Shenyang Agricultural UniversityShenyang 110866P.R.China

Wheat stem rust, caused by Puccinia graminis f. sp. tritici(Pgt), is a potentially devastating fungal disease of wheat worldwide. The present study was to evaluate the resistance of 42 wheat monogenic lines with known stem rust resistance(Sr) genes and 69 wheat cultivars to three new Pgt races(34C0MRGQM, 34C3MKGQM, and 34C6MTGSM)identified from aeciospores at the seedling and adult-plant stages. The phenotyping results revealed that monogenic lines harboring resistance genes Sr9e, Sr17, Sr21, Sr22, Sr26, Sr30, Sr31, Sr33, Sr35, Sr36, Sr37, Sr38, Sr47, SrTmp,and SrTt3 were effectively resistant to all three Pgt races at the seedling and adult-plant stages. In contrast, monogenic lines containing Sr5, Sr6, Sr7b, Sr9a, Sr9d, Sr9f, Sr9g, Sr9b, Sr16, Sr24, Sr28, and Sr39 were highly susceptible to these races at both seedling and adult-plant stages. The other lines with Sr8a, Sr10, Sr11, Sr13, Sr14, Sr15, Sr18, Sr20,Sr19, Sr23, Sr25, Sr27, Sr29, Sr32, and Sr34, displayed variable levels of resistance to one or two of the tested *** infection types(ITs) and adult-plant infection responses(IRs) indicated that 41(59.4%) of the wheat cultivars showed high resistance to all the three races. Molecular marker analysis showed that four wheat culitvars likely carried Sr2, 20 wheat culitvars likely carried Sr31, 9 wheat culitvars likely carried Sr38, and none of the cultivars carried Sr24,Sr25, and Sr26. Our results provide a scientific basis for rational utilization of the tested Sr genes and wheat cultivars against these novel Pgt races.

关键词： wheat stem rust Puccinia graminis f.sp.tritici wheat cultivars resistance genes

互联网使用促进农户参与“粮改饲”了吗?--基于农牧交错带农户调研的微观证据

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中国农业大学学报 2024年第3期29卷 287-300页

作者：张文娥罗宇高军军彭凌志赵敏娟西北农林科技大学经济管理学院陕西杨凌712100 中国农业大学经济管理学院北京100083 神木市畜牧业发展中心陕西神木719300

在互联网强国战略背景下,基于农牧交错带陕西和甘肃两省1 479份农户的调研数据,运用Probit模型和条件混合过程估计方法(CMP),探究互联网使用对农户参与“粮改饲”的影响,并关注不同养殖规模和不同人力资本禀赋条件下互联网使用对农户参... 详细信息

在互联网强国战略背景下,基于农牧交错带陕西和甘肃两省1 479份农户的调研数据,运用Probit模型和条件混合过程估计方法(CMP),探究互联网使用对农户参与“粮改饲”的影响,并关注不同养殖规模和不同人力资本禀赋条件下互联网使用对农户参与“粮改饲”影响的异质性。结果表明:1)互联网使用提高了农户参与“粮改饲”的可能性,即在给定其他变量不变的情况下,有互联网使用行为的农户参与“粮改饲”的几率比是没有互联网使用行为农户的38.82%,该结果在经过子样本检验和更换核心解释变量检验后依然稳健。2)异质性估计结果表明,互联网使用对农户参与“粮改饲”的影响在不同养殖规模和不同人力资本禀赋条件下存在差异,即非规模养殖户和低人力资本禀赋的农户家庭更容易通过使用互联网参与“粮改饲”。由此提出,完善农牧交错地区互联网利用配套设施,提高农户农业经营的网络化水平,实施差别化的互联网扶持措施等政策建议。

关键词：互联网使用农户参与“粮改饲” 农牧交错带条件混合过程估计(CMP) 异质性

Comprehensive and high-resolution emission inventory of atmospheric pollutants for the northernmost cities agglomeration of Harbin-Changchun,china:Implications for local atmospheric environment management

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Journal of Environmental sciences 2021年第6期33卷 150-168页

作者： Mengduo Zhang Weiwei Chen Xiangjin Shen Hongmei zhao Chengkang gao xuelei Zhang Wei Liu Chengjiang yang yang Qin Shichun Zhang Jing Fu Daniel Tong Aijun Xiu Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China university of Chinese Academy of sciences Beijing 100049China Northeastern university School of Metallurgy Shenyang 110819China Heilongjiang Provincial Academy of Environmental sciences Harbin 150090China Jilin Provincial Ecological Environment Monitoring Center Changchun 130012China Center for Spatial Information science and Systems George Mason UniversityFairfaxVA 22030USA Cooperative Institute for Climate&Satellites University of MarylandCollege ParkMD 20740USA

Using a bottom-up estimation method,a comprehensive,high-resolution emission inventory of gaseous and particulate atmospheric pollutants for multiple anthropogenic sectors with typical local sources has been developed for the Harbin-Changchun city agglomeration(HCA).The annual emissions for CO,NO_(x),SO_(2),NH_(3),VOC S,PM_(2.5),PM 10,BC and OC during 2017 in the HCA were estimated to be 5.82 Tg,0.70 Tg,0.34 Tg,0.75 Tg,0.81 Tg,0.67 Tg,1.59 Tg,0.12 Tg and 0.26 Tg,*** PM 10 and SO_(2),the emissions from industry processes were the dominant contributors representing 54.7%and 49.5%,respectively,of the total emissions,while 95.3%and 44.5%of the total NH_(3)and NO x emissions,respectively,were from or associated with agricultural activities and *** distributions showed that most emissions(except NH_(3))occurred in November to March and were concentrated in the central cities of Changchun and Harbin and the surrounding *** burning of straw made an important contribution to PM_(2.5)in the central regions of the northeastern plain during autumn and spring,while domestic coal combustion for heating purposes was significant with respect to SO_(2)and PM_(2.5)emissions during autumn and ***,based on Principal Component Analysis and Multivariable Linear Regression model,air temperature,relative humidity,electricity and energy consumption,and the urban and rural population were optimized to be representative indicators for rapidly assessing the magnitude of regional atmospheric pollutants in the *** indicators and equations were demonstrated to be useful for local atmospheric environment management.

关键词： Anthropogenic emissions Inventory Air quality Atmospheric pollution Environmental management Northern china

气候变化背景下我国天麻种植适宜性和适宜区的评估

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作物学报 2024年第4期50卷 1004-1014页

作者：郝佳乐赵炯超赵明宇王艺璇逯杰石晓宇高真真褚庆全中国农业大学农学院/农业农村部农作制度重点实验室北京100193

气候变化通过改变物种栖息地的适宜性来影响物种的生长区域。天麻作为中国珍稀濒危药用植物,评估其种植适宜区分布以及气候变化对天麻种植适宜性的影响可以为天麻生产布局提供重要依据。本研究采用MaxEnt模型,基于我国天麻地理分布数据... 详细信息

气候变化通过改变物种栖息地的适宜性来影响物种的生长区域。天麻作为中国珍稀濒危药用植物,评估其种植适宜区分布以及气候变化对天麻种植适宜性的影响可以为天麻生产布局提供重要依据。本研究采用MaxEnt模型,基于我国天麻地理分布数据和环境数据,模拟天麻种植适宜性和适宜区的空间分布和变化。结果表明,影响天麻分布的主要环境因子是5月至7月太阳辐射量、10月至11月降水量、最冷月最低温和植被类型。我国天麻种植高适宜区主要分布在四川盆地周围的西南地区。1961—2020年,天麻种植适宜性呈波动增加趋势,种植适宜性提高的面积占全国陆地面积的9.10%,主要分布在西南地区、华中、华东的部分地区以及陕西省。过去60年间5月至7月太阳辐射量总体上的下降是天麻种植适宜性提高的主要原因。本研究通过对天麻种植适宜性准确评估和种植适宜区变化因子解析,为我国天麻生产与人工栽培选址提供科学依据,对制定应对未来气候变化的对策具有借鉴意义。

关键词：天麻种植适宜性环境因子 MaxEnt ArcGIS

集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应

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微生物学报 2018年第10期58卷 1732-1742页

作者：赵佳琳陈军崔玉琳于淑贤陈高秦松中国科学院烟台海岸带研究所山东烟台264003 中国科学院大学北京100049 山东省农业科学院生物技术研究中心山东济南250100 山东省作物遗传改良与生态生理重点实验室山东济南250100

丝氨酸/苏氨酸激酶是蓝藻感知和转导外界刺激的重要元件,但至今蓝藻中很多丝氨酸/苏氨酸激酶的功能尚属未知。【目的】研究集胞藻PCC6803中的丝氨酸/苏氨酸激酶Spk C是否参与对高温胁迫的响应。【方法】本研究采用同源重组的方法构建sp... 详细信息

丝氨酸/苏氨酸激酶是蓝藻感知和转导外界刺激的重要元件,但至今蓝藻中很多丝氨酸/苏氨酸激酶的功能尚属未知。【目的】研究集胞藻PCC6803中的丝氨酸/苏氨酸激酶Spk C是否参与对高温胁迫的响应。【方法】本研究采用同源重组的方法构建spC基因完全敲除突变株,检测突变株与野生株在高温胁迫下的生长状况、色素组成,并对高温胁迫下叶绿素荧光参数差异进行分析,比较光合系统Ⅱ活性差异。此外,通过测定生长速率来判断高温胁迫后藻株的恢复情况。【结果】经过42℃高温胁迫后,与野生株相比,突变株ΔspkC生长减缓,光合色素(叶绿素、类胡萝卜素和藻胆色素)的含量降低;45℃高温胁迫下突变株ΔspkC的光合系统Ⅱ活性下降幅度更大;经过5 d 42℃高温处理后,突变株生长几乎停滞,存活率较野生株明显降低。【结论】集胞藻PCC 6803中spkC基因的缺失导致突变株对高温胁迫响应出现缺陷,提示丝氨酸/苏氨酸激酶SpkC参与响应高温胁迫。

关键词：集胞藻PCC 6803 丝氨酸/苏氨酸激酶SpkC 高温胁迫