检索结果-南通市图书馆

Effect of liver regeneration after partial hepatectomy and ischemia-reperfusion on expression of growth factor receptors

引用

World Journal of gastroenterology 2006年第24期12卷 3835-3840页

作者： P Baier g wolf-vorbeck S Hempel UT Hopt E von Dobschuetz Department of general and Visceral Surgery Albert-Ludwigs-University FreiburgGermany

AIM： To investigate the effects of experimental partial hepatectomy and normothermic ischemia-reperfusion damage on the time course of the expression of four different growth factor receptors in liver regeneration. This is relevant due to the potential therapeutic use of growth factors in stimulating liver regeneration. METHODS： For partial hepatectomy （PH） 80% of the liver mass was resected in Sprague Dawley rats. Ischemia and reperfusion （I/R） were induced by occlusion of the portal vein and the hepatic artery for 15 min. The epidermal growth factor receptor, hepatic growth factor receptor, fibroblast growth factor receptor and tumour necrosis factor receptor-1 were analysed by immunohistochemistry up to 72 h after injury. Quantitative RT-PCR was performed at the time point of minimal receptor expression （24 h）. RESULTS： In immunohistochemistry, EgFR, HgFR, FgFR and TNFR1 showed biphasic kinetics after partial hepatectomy with a peak up to 12 h, a nadir after 24 h and another weak increase up to 72 h. During liver regeneration, after ischemia and reperfusion, the receptor expression was lower; the nadir at 24 h after reperfusion was the same. To evaluate whether this nadir was caused by a lack of mRNA transcription, or due to a posttranslational regulation, RT-PCR was performed at 24 h and compared to resting liver. In every probe there was specific mRNA for the receptors. EgFR, FgFR and TNFR1 mRNA expression was equal or lower than in resting liver, HgFR expression after I/R was stronger than in the control. CONCLUSION： At least partially due to a post-transcriptional process, there is a nadir in the expression of the analysed receptors 24 h after liver injury. Therefore, a therapeutic use of growth factors to stimulate liverregeneration 24 h after the damage might be not successful.

关键词： Partial hepatectomy Liver ischemia growthfactor receptors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Electro-Hydrodynamic(EHD) Thrust Analysis in Wire-Cylinder Electrode Arrangement

引用

Plasma Science and Technology 2014年第4期16卷 363-369页

作者： Konstantinos N.KIOUSIS Antonios X.MORONIS wolf g.FRUH Heriot-Watt University Technological Educational Institute(TEI) of Athens

The thrust generation by electro-hydrodynamic （EHD） effect has been studied for a wire-cylinder arrangement under high DC voltage. Series of measurements have been conducted in order to determine the relationship between generated thrust and corona discharge current, as well as its dependence on geometrical characteristics of the electrodes, e.g. electrode gap, wire and cylinder radii. The experimental investigation has shown a linear relationship between the generated thrust and the discharge current, while parametric analysis showed that increased electrode gap and emitter radius reduces the thrust. On the other hand, large gaps favor the thrust per unit power ratio.

关键词： electro-hydrodynamic (EHD) effect thrust wire-cylinder electrodes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tempering of exhausted T cells to comprehend their adaptive response for suitable clinical translation

引用

Cellular & Molecular Immunology 2023年第12期20卷 1401-1402页

作者： Amit Sharma Ingo g.H.Schmidt-wolf Department of Integrated Oncology Center for Integrated Oncology(CIO)BonnUniversity Hospital BonnBonnGermany

Although cancer immunotherapy has taken center stage,exhaustion of T cells is undeniably one of the major problems preventing it from being fully successful[1].Certainly,reversal of T-cell exhaustion or dysfunction is of great importance for developing new strategies and restoring immune ***,counterarguments about the adverse effect of reversing T-cell exhaustion(leading to overactivation of T cells and cytotoxicity)and even their irreversible properties have been quite common in scientific *** more to be discovered about the reasons for T-cell failure,a better understanding of the molecular factors/determinants[2,3]that shape/remodel“heterogeneous”dysfunctional T cells in cancer makes clear ***,two recent publications in Nature immunology have described the intriguing perspective of T-cell exhaustion[4,5].Of interest,Vignali et *** on the hypoxia-driven CD39-dependent suppressor function of exhausted T cells,whereas Saadey et *** restoring the epigenetic balance of TgFβ1/BMP signaling in exhausted T cells to unlock responsiveness to immune checkpoint blockade therapy.

关键词： irreversible exhaustion publications

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Data-driven prediction of plate velocities and plate deformation of explosive reactive armor

引用

Defence Technology（防务技术） 2022年第12期18卷 2141-2149页

作者： Marvin Becker Andreas Klavzar Thomas wolf Melissa Renck Institute of Saint-Louis 5 Rue du Général Cassagnou68330Saint LouisCedexFrance Ecole Centrale de Lille CitéScientifique59651Villeneuve d'AscqCedexFrance

Explosive reactive armor(ERA)is currently being actively developed as a protective system for mobile devices against ballistic threats such as kinetic energy penetrators and shaped-charge *** mobility,the aim is to design a protection system with a minimal amount of required *** efficiency of an ERA is sensitive to the impact position and the timing of the ***,different designs have to be tested for several impact scenarios to identify the best *** analytical models are not predicting the behavior of the ERA accurately enough and experiments,as well as numerical simulations,are too time-consuming,a data-driven model to estimate the displacements and deformation of plates of an ERA system is proposed *** ground truth for the artificial neural network(ANN)is numerical simulation results that are validated with *** ANN approximates the plate positions for different materials,plate sizes,and detonation point positions with sufficient accuracy in *** a future investigation,the results from the model can be used to estimate the interaction of the ERA with a given ***,a measure for the effectiveness of an ERA can be ***,an optimal ERA can be designed and analyzed for any possible impact scenario in negligible time.

关键词： Artificial neural network Explosive reactive armor Finite element simulation Particle simulation Flash X-ray

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Towards Accurate Bit Error Simulation in Wireless Sensor Networks Including Environmental Influences

引用

Journal of Computer Science & Technology 2020年第4期35卷 809-824页

作者： Sven Pullwitt Robert Hartung Ulf Kulau Lars wolf Institute for Operating Systems and Computer Networks Technische Universitat BraunschweigBraunschweig 38106 Germany

Simulation is a common technique for the evaluation of new approaches and protocols in networked systems and provides many ***,it is also well known that the relevance of the simulation results for real-world applications depends on the various models which are used within the simulation,e.g.,for the characteristics of the radio *** this paper,we introduce the Extended Multipath Raytracing Model,an extension to the ray-tracing radio medium available in Cooja,to improve the modelling of wireless links in simulated Wireless Sensor *** extension allows the simulation of environmental influences onto links on a per node basis,allowing the analysis of various effects observed in experiments in a virtual ***,the packet-based modelling of transmission errors is extended to provide the simulation of bit errors,allowing new usage scenarios,like the simulation of error detection and Forward Error Correction codes in Cooja.

关键词： bit error Contiki Cooja environmental influence simulation wireless sensor network

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Theories and Simulations in Substorm Research:A Review

引用

空间科学学报 2011年第2期31卷 125-149页

作者： R.A.wolf Physics and Astronomy Department Rice UniversityHoustonTexas77251-1892USA

Both theory and simulation have played important roles in defining and illuminating the key mechanisms involved in *** theories of magnetic reconnection and of interchange and ballooning instabilities were developed more than 50 years ago,and these plasma physical concepts have been central in discussions of substorm physics.A vast amount of research on reconnection,including both theoretical and computational studies,has helped provide a picture of how reconnection operates in the collisionless environment of the ***,however,we do not fully understand how key microscale processes and large-scale dynamics work together to determine the location and rate of *** in the last twenty years,it has become clear that interchange processes are important for transporting plasma through the plasma sheet in the form of bursty bulk flows and substorm expansions,we still have not reached the point where simulations are able to realistically and defensibly represent all of the important aspects of the *** than two decades ago it was suggested that the ballooning instability,the basic theory for which dates from the 1950s,may play an important role in *** the majority of experts agree that regions of the plasma sheet are often linearly unstable to ideal-MHD ***,it is also clear that kinetic effects introduce important modifications to the MHD stability *** is still uncertain whether ballooning plays a leading role in substorms or has just a minor *** the different types of simulations that have been applied to the substorm problem,global MHD codes are unique in that,in a sense, they represent the entire global substorm phenomenon,including coupling to the solar wind and ionosphere, and the important mechanisms of reconnection,interchange,and ***,they have not yet progressed to the point where they can accurately represent the whole phenomenon,because grid-resolution proble

关键词： Substorm Reconnection Interchange Simulations

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Herb Formulae Inhibit the Proliferation of Human Colon Cancer SW480 Cells by Inducing Cell Apoptosis

引用

World Journal of Traditional Chinese Medicine 2023年第3期9卷 348-354页

作者： Su Fu Shao-Lin Qin wolf-Dieter Rausch Department for Internal Medicine of Traditional Chinese Medicine Eye Hospital China Academy of Chinese Medical SciencesBeijingChina Department for Biomedical Sciences Institute for Chemistry and BiochemistryUniversity of Veterinary Medicine ViennaViennaAustria

Objective:This study aimed to reveal the antitumor effects of Chinese herbal formulae and the underlying mechanisms in treating colorectal cancer,with a focus on developing traditional Chinese medicine(TCM)as a supplement and alternative therapeutic method for *** and Methods:Human colon cancer SW480 cells were treated with three Chinese herbal formulae,Bu Zhong Yi Qi Decoction,Fuzi Lizhong Decoction,and Pulsatilla Decoction at different concentrations(50–600μg/mL)for 24,36,and 48 h,*** viability was determined using the resazurin reduction assay,and cell survival rate was evaluated using a colony formation *** treatment with different concentrations(50–600μg/mL)of these three formulae for 48 h,the effects of the Chinese herbal formulae on cell apoptosis were investigated using Hoechst/propidium iodide(PI)*** positive PI-stained cells were investigated using an EnSpire multilabel plate reader and the positive Hoechst-stained cells were observed under a fluorescence microscope for morphological ***:Bu Zhong Yi Qi Decoction,Fuzi Lizhong Decoction,and Pulsatilla Decoction inhibited SW480 cell proliferation in a dose-and time-dependent manner and induced cell ***:Chinese herbal formulae with a special prescription form of TCM with antitumor effects bring a new perspective in line with the principles of TCM in cancer treatment.

关键词： Apoptosis cell proliferation Chinese herbal formulae colorectal cancer SW480 cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pharmacological drug screening to inhibit uveal melanoma metastatic cells either via EgF-R, MAPK, mTOR or PI3K

引用

International Journal of Ophthalmology(English edition) 2022年第10期15卷 1569-1576页

作者： Stefan Kassumeh Sebastian Arrow AndréKafka Nikolaus Luft Siegfried g.Priglinger Armin wolf Kirsten Eibl-Lindner Christian M.Wertheimer Department of Ophthalmology University HospitalLMU MunichMunich 80336Germany Department of Ophthalmology University HospitalUniversity UlmUlm 89075Germany

AIM: To screen five potential pharmacological substances specifically targeting EgF-R, MAPK, mTOR, or PI3K for their antiproliferative effects, possible impact on cell viability, as well as cell death rates on three different uveal melanoma metastasis cell lines in vitro. METHODS: Three different uveal melanoma metastasis cell lines(OMM2.5, OMM2.3, and OMM1), that originated from human hepatic and subcutaneous metastasis, were exposed to inhibitors of different targets: erlotinib(EgF-R), everolimus(mTOR), selumetinib(MAPK), trametinib(MAPK) or the alkylphosphocholine erufosine(PI3K). Cell viability was assessed with a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide(XTT) dye reduction assay after 24 h of treatment. Antiproliferative effects were evaluated separately after a 72-hour incubation of the cells with the pharmacological substance. Subsequently, the IC_(50) was calculated. Tumor cell death was investigated using a double stain apoptosis detection assay. RESULTS: Selumetinib, trametinib, and erufosine significantly decreased cell viability of all OMM cell lines(Pgnificant inhibition of cell proliferation(Pges in the number of dead cells for the other substances were ***: The preliminary drug screening demonstrates five new candidates, successfully targeting the canonical MAPK/ERK and PI3K/AKT/m TOR pathways in uveal melanoma metastasis cells in vitro. Hence, these findings provide an experimental basis to explore future single or combined therapy strategies for metastatic uveal melanoma.

关键词： uveal melanoma selumetinib trametinib erufosine erlotinib

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Eosinophilic cholangitis is a potentially underdiagnosed etiology in indeterminate biliary stricture

引用

World Journal of gastroenterology 2017年第6期23卷 1044-1050页

作者： Dirk Walter Sylvia Hartmann Eva Herrmann Jan Peveling-Oberhag wolf O Bechstein Stefan Zeuzem Martin-Leo Hansmann Mireen Friedrich-Rust Jorg g Albert Department of Internal Medicine I Johann Wolfgang Goethe-University Hospital Dr.Senckenberg Institute for Pathology Johann Wolfgang Goethe-University Hospital Institute of Biostatistics and Mathematical Modeling Johann Wolfgang Goethe-University Hospital Department for gastroenterology Hepatology and EndocrinologyRobert-Bosch Hospital Department of general and Visceral Surgery Johann Wolfgang Goethe-University Hospital

To investigate presence and extent of eosinophilic cholangitis (EC) as well as Igg4-related disease in patients with indeterminate biliary stricture (IBS).METHODSAll patients with diagnosis of sclerosing cholangitis (SC) and histopathological samples such as biopsies or surgical specimens at University Hospital Frankfurt from 2005-2015 were included. Histopathological diagnoses as well as further clinical course were reviewed. Tissue samples of patients without definite diagnosis after complete diagnostic work-up were reviewed regarding presence of eosinophilic infiltration and Igg4 positive plasma cells. Eosinophilic infiltration was as well assessed in a control group of liver transplant donors and patients with primary sclerosing *** hundred and thirty-five patients with SC were included. In 10/135 (13.5%) patients, no potential cause of IBS could be identified after complete diagnostic work-up and further clinical course. After histopathological review, a post-hoc diagnosis of EC was established in three patients resulting in a prevalence of 2.2% (3/135) of all patients with SC as well as 30% (3/10) of patients, where no cause of IBS was identified. 2/3 patients with post-hoc diagnosis of EC underwent surgical resection with suspicion for malignancy. Diagnosis of Igg4-related cholangitis was observed in 7/135 patients (5.1%), whereas 3 cases were discovered in post-hoc analysis. 6/7 cases with Igg4-related cholangitis (85.7%) presented with eosinophilic infiltration in addition to Igg4 positive plasma cells. There was no patient with eosinophilic infiltration in the control group of liver transplant donors (n = 27) and patients with primary sclerosing cholangitis (n = 14).CONCLUSIONEC is an underdiagnosed benign etiology of SC and IBS, which has to be considered in differential diagnosis of IBS.

关键词： Indeterminate biliary stricture endoscopy endoscopic retrograde cholangiopancreatography eosinophilic cholangitis bile duct stenosis Igg4-related disease primary sclerosing cholangitis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A deep learning-enabled portable imaging flow cytometer for cost-effective, highthroughput, and label-free analysis of natural water samples

引用

Light(Science & Applications) 2018年第1期7卷 416-427页

作者： Zoltán gӧrӧcs Miu Tamamitsu Vittorio Bianco Patrick wolf Shounak Roy Koyoshi Shindo Kyrollos Yanny Yichen Wu Hatice Ceylan Koydemir Yair Rivenson Aydogan Ozcan Electrical and Computer Engineering Department University of CaliforniaLos AngelesCA 90095USA Bioengineering Department University of CaliforniaLos AngelesCA 90095USA California NanoSystems Institute(CNSI) University of CaliforniaLos AngelesCA 90095USA

We report a deep learning-enabled field-portable and cost-effective imaging flow cytometer that automatically captures phase-contrast color images of the contents of a continuously flowing water sample at a throughput of 100 mL/*** device is based on partially coherent lens-free holographic microscopy and acquires the diffraction patterns of flowing micro-objects inside a microfluidic *** holographic diffraction patterns are reconstructed in real time using a deep learning-based phase-recovery and image-reconstruction method to produce a color image of each micro-object without the use of external *** blur is eliminated by simultaneously illuminating the sample with red,green,and blue light-emitting diodes that are *** by a laptop computer,this portable device measures 15.5 cm×15 cm×12.5 cm,weighs 1 kg,and compared to standard imaging flow cytometers,it provides extreme reductions of cost,size and weight while also providing a high volumetric throughput over a large object size *** demonstrated the capabilities of this device by measuring ocean samples at the Los Angeles coastline and obtaining images of its micro-and nanoplankton ***,we measured the concentration of a potentially toxic alga(Pseudo-nitzschia)in six public beaches in Los Angeles and achieved good agreement with measurements conducted by the California Department of Public *** cost-effectiveness,compactness,and simplicity of this computational platform might lead to the creation of a network of imaging flow cytometers for largescale and continuous monitoring of the ocean microbiome,including its plankton composition.

关键词： flow holographic simplicity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：