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Candidate genes underlying QTL for flowering time and their interactions in a wide spring barley(Hordeum vulgare L.) cross

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The Crop journal 2021年第4期9卷 862-872页

作者： Ana M.Casas Carlota R.Gazulla Arantxa Monteagudo Carlos P.Cantalapiedra Marian Moralejo MPilar Gracia francisco j.Ciudad William T.B.Thomas jose L.Molina-Cano Scott Boden Bruno Contreras-Moreira Ernesto Igartua Department of Genetics and Plant Production Aula Dei Experimental Station(EEAD-CSIC)Avda Montanana 1005E-50059 ZaragozaSpain University of Lleida-AGROTECNIO Center Avda.Rovira Roure 19125198 LleidaSpain Instituto de Tecnologia Agraria Junta de Castilla y LeonITACyLP.O.Box 17247071 ValladolidSpain The james Hutton Institute InvergowrieDundee DD25DAUK Institut de Recerca i Tecnologia Agroalimentaries(UdL-IRTA) Avda Rovira Roure 19125198 LleidaSpain john Innes Centre Norwich Research ParkNorwich NR47UHUK Fundacion ARAID calle Maria de Luna 1150018 ZaragozaSpain Present address:Centro de Biotecnologia y Genomica de Plantas Universidad Politecnica de Madrid(UPM)and Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria(INIA)Madrid 28223Spain Present address:School of Agriculture Food and WineWaite Research InstituteUniversity of AdelaideGlen OsmondSA 5064Australia Present address:The European Bioinformatics Institute(EMBL-EBI) Wellcome Trust Genome CampusHinxtonUK

Response to vernalization and photoperiod are the main determinants controlling the time to flowering in temperate cereals. While the individual genes that determine a plant's response to these environmental signals are well characterized, the combinatorial effect on flowering time of allelic variants for multiple genes remains unresolved. This study investigated the genetic control of flowering-time in a biparental population of spring barley, derived from a wide cross between a late-flowering European and an early-flowering North-American cultivar. While the major flowering time genes are not segregating in the Beka ×Logan cross, large variation in flowering was observed. We identified five QTL, with both parents found to contribute early alleles. The catalog of QTL discovered aligns with several candidate genes affecting flowering time in barley. The combination of particular alleles at HvCEN, HvELF3 and HvFT1 in Logan are responsible for the earliness of this cultivar. Interestingly, earliness for flowering could be further enhanced, with Beka found to contribute three early alleles, including a QTL colocating with a HvFD-like gene, suggesting that there are diverse aspects of the flowering-time pathway that have been manipulated in these two cultivars. Epistatic interactions between flowering-time QTL or candidate genes were observed in field data and confirmed under controlled conditions. The results of this study link photoperiod-dependent flowering-time genes with earliness per se genes into a single model, thus providing a unique framework that can be used by geneticists and breeders to optimize flowering time in barley.

关键词： Barley Flowering HvELF3 HvCEN HvFT1

Luminometric Studies of Yeast Response to Complex Environmental Calcium Variations Demonstrate Sensing of External Calcium Ion Changes

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Advances in Microbiology 2022年第9期12卷 555-567页

作者： Augustus C. Parker David A. Mintz francisco j. Solis Pamela A. Marshall School of Mathematical and Natural Sciences Arizona State University Glendale USA

The response of yeast to sharp environmental increases in calcium concentration has been extensively studied. However, systematic studies of the response under more general changes are still lacking. Only limited exploration of cellular responses has been conducted where calcium concentration is decreased. This article describes a set of luminometric experiments that monitor the cytosolic calcium concentration under changing external concentration conditions. As a decrease in external calcium concentrations requires the use of large sample volumes, the experiments require the use of equipment adapted for this purpose. We describe the modification of commercial luminometric equipment to make the exploration possible. We explore the yeast cellular behavior when an increase in external calcium concentration is followed by a decrease in external calcium concentration. We compare these results with those from the case of a double pulse of concentration increase. Results from the experiment show that the first, concentration increasing pulse produces the well-known sharp increase in cytosolic calcium followed by calcium sequestration to return to a cytosolic concentration near its initial condition. Surprisingly, the calcium decrease step shows similar results with a cytosolic increase followed by a return to lower levels. The results suggest the presence of a calcium sensing mechanism regulating calcium influx from external sources. This mechanism would produce channel opening as a response to any changes in external concentration, be it an enhancement or a depletion.

关键词： Saccharomyces cerevisiae Calcium Homeostasis Extracellular Ion Changes

Community acquired infections and risk factors in a rheumatologic ward

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Community acquired infections and risk factors in a rheumato...

An Unexpected Presentation of Tuberculosis in the Surgical Field with a Preventable Disease Transmission

The 8th International Congress on SLE

作者： Mora Claudia S Segami María I Mendez francisco j Hospital Edgardo Rebagliati M artins-ESSALUD Hospital Edgardo Rebagliati Martins-ESSALUD Universidad Nacional Mayor de San Marcos Universidad Peruana Cayetano Heredia Lima-Perú

＜正＞Purpose:To investigate community acquired infection associated risk factors in patients admitted to a rheumatologic ward. Method:Clinical records of 220 patients with rheumatologic autoimmune diseases hospitaliz...

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Surgical Science 2018年第11期9卷 407-411页

作者： Hector M. Lopez Paloma M. Lugo francisco j. jacome School of Medicine University of Medicine and Health Sciences Basseterre Saint Kitts and Nevis Department of Surgery Doctors Hospital Augusta GA USA Department of Surgery University Hospital Summerville Augusta GA USA

Tuberculosis (TB) incidence within Healthcare workers remains one of the lowest occupation specific rates in the United States. We report a case of a 33-year-old African American male presented with a right chest mass and without fever, chills or cough. Bacterial and Fungal cultures were sent during the initial visit and were negative throughout the entire management of his care. After several months of surgical interventions, the patient appeared positive for TB;however a staff member contracted the disease. The patient was managed successfully with the four-drug regimen of Isoniazid, Rifampin, Pyrazinamide and Ethambutol and recovered well from his surgeries.

关键词： Tuberculosis Transmission Surgical Field Prevention

The Tension between New Media and Narratology： The Case of Cinema

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journal of Philosophy Study 2012年第9期2卷 677-687页

作者： Alberto j. L. Carrillo, Marco Calder6n, francisco j. Montes May Zindel Benem6rita Universidad Aut6noma de Puebla Universidad del Arte

We will discuss here the tension between the reality of new media and narratological theory, and the case in point will be cinema. Our thesis is that new media have to do with illusion, basically with illusion, and only derivatively with imagination; on the contrary, the field of literature has to do with imagination, strictly with imagination, not with illusion at all. If it is so, something must be wrong with the pretence of narratology, which has literature as its basic referent, to be the adequate theoretical frame in order to understand every cultural phenomenon, especially in the case of new media. We have to insist on the distinction between illusion and imagination. In fact, most literary theorists do not bear in mind such distinction; it tends to completely disappear under the general title ＂fiction.＂ To be sure, it is usually said of cinema that it is fiction, like literature, but cinema is much more than fiction, it is illusion, even if it is the illusion of a story.

关键词： imagination illusion plastic imagination aesthetic belief sameness of the character

Integrating species and successional classes for wood production in a mixed forest restoration system in a neotropical region

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journal of Forestry Research 2021年第6期32卷 2313-2321页

作者： Ananias francisco Dias junior Ana Lucia Martins Sodero Pincelli Ana Paula Camara da Silva Alison Moreira da Silva Natalia Dias de Souza Mario Tommasiello Filho jose Otavio Brito Wood and Forestry Science Department Federal University of EspIrito Santo(UFES)Av.Governador Lindemberg316JerOnimo Monteiro CityES 29550-000Brazil São Paulo State School of Technology(FATEC/SP) Av.DiacOno Jair de Oliveira651PiracicabaSP 13414-141Brazil Forest Products Department Federal Rural University of Rio de Janeiro(UFRRJ)BR 465km 07SeropEdicaRJ 29890-000Brazil University of SAo Paulo “Luiz de Queiroz”College of Agriculture(USP/ESALQ)Av.PAdua Dias11PiracicabaSP 13418-900Brazil

As forests in neotropical regions,particularly in developing countries,are devastated,interventions to restore biodiversity and its ecological functions are *** producers have thus been encouraged to grow trees for wood production as an economic *** objective of this study was to quantify the increment of wood density of four forest species from different successional classes of a mixed system of restoration in a neotropical forest in *** discs were sampled at breast height and analyzed radially by X-ray densitometry to obtain apparent density and basic *** trees each of a species from the pioneer,early and late secondary successional stages and of the dominant species in the climax *** radial profiles indicated increasing density from the pith to bark of trunks,except for some variations due to wood defects and *** density was 0.576 g cm^(-3),0.655 g cm^(-3),0.706 g cm^(-3)and 0.775 g cm^(-3),respectively,for Peltophorum dubium,Schinus terebinthifolius,Cariniana estrellensis and Hymenaea *** profiles indicated higher amplitudes in the apparent densities for slow-growing species.X-ray densitometry generated parameters such as minimum,average and maximum densities,and radial density *** parameters are important for understanding the ecological functional role of successional classes of the Atlantic Forest from the Neotropical region.

关键词： X-ray densitometry Timber species of neotropical forest Successional classes of forest species Maintenance of forest restoration

Prevalence of and risk factors for erectile dysfunction in young nondiabetic obese men:results from a regional study

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Asian journal of Andrology 2020年第4期22卷 372-378页

作者： María Molina-Vega Maite Asenjo-Plaza María joséBanderas-Donaire María Dolores Hernández-Ollero Silvia Rodríguez-Moreno juan jálvarez-Millán Pablo Cabezas-Sanchez Fernando Cardona-Díaz juan Alcaide-Torres Lourdes Garrido-Sánchez Daniel Castellano-Castillo francisco j Tinahones joséC Fernández-García Department of Endocrinology and Nutrition Virgen de la Victoria University Hospital(IBIMA)Málaga UniversityMálaga 29010Spain Centro de Investigación Biomédica en Red de la Fisiopatología de la Obesidad y Nutrición(CIBEROBN) Instituto de Salud Carlos IIIMadrid 28029Spain Cruz de Humilladero Primary Care Center Málaga 29010Spain Teatinos Primary Care Center Málaga 29010Spain Chemical Sanitary Consulting(CQS Lab) Madrid 28003Spain

Erectile dysfunction(ED),a condition closely related to cardiovascular morbidity and mortality,is frequently associated with *** this study,we aimed to determine the prevalence of ED and evaluate the associated risk factors in a cohort of 254 young(18–49 years)nondiabetic obese(body mass index[BMI]≥30 kg m−2)men from primary *** function(International Index of Erectile Function[IIEF-5]questionnaire),quality of life(Aging Males'Symptoms[AMS scale]),and body composition analysis(Tanita MC-180MA)were *** testosterone was determined using high-performance liquid chromatography–mass *** logistic regression analysis was used to study the factors associated with *** prevalence was 42.1%.Subjects with ED presented higher BMI,waist circumference,number of components of the metabolic syndrome,AMS score,insulin resistance,and a more unfavorable body composition than those without *** logistic regression analysis showed that a pathological AMS score(odds ratio[OR]:4.238,P40%of subjects.A pathological AMS score,the degree of obesity,and age were positively associated with ED,while elevated HDL-cholesterol levels were inversely associated with the odds of presenting *** prospective studies are needed to evaluate the long-term consequences of ED in this population.

关键词： Aging Males'Symptoms score erectile dysfunction International Index of Erectile Function-5 questionnaire obesity testosterone

Study of the Reactivity of (100) Felodipine Surface Model Based on DFT Concepts

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Open journal of Physical Chemistry 2019年第1期9卷 1-12页

作者： Carlos Tepech-Carrillo Roxana Licona-Ibarra j. francisco Rivas-Silva Antonio Flores-Riveros Instituto de Física “Luis Rivera Terrazas” Benemérita Universidad Autónoma de Puebla Puebla México Facultad de Ciencias Químicas Benemérita Universidad Autónoma de Puebla Puebla Mexico

In this study, Density Functional Theory including a dispersion correction is employed to model and analyze the structural, electronic and local reactivity of the (100) surface of felodipine. The surface energy calculated at the Generalized Gradient Approximation (GGA) level, along with plane waves as basis set and ultrasoft pseudopotentials, shows that the (100) surface is the most stable as compared to the (010) and (110) ones. In particular, we have focused on performing a quantitative study of the reactivity of the surface by means of the Fukui function and through the HOMO and LUMO populations. Our results can be related to some applications in the pharmaceutical chemistry of this compound.

关键词： 1 4-Dihydropyridine Felodipine Density Functional Theory Fukui Functions

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三维影像重建在泌尿外科机器人手术中的应用

中华泌尿外科杂志 2018年第9期39卷 690-693页

作者：张凯朱刚李鸿波 francisco .Martinez ortillo 北京和睦家医院泌尿外科北京100015

目的探讨三维影像重建在泌尿外科机器人手术中的应用价值。方法回顾性分析2017年9月至2018年6月收治的7例泌尿系肿瘤患者的病例资料。男4例，女3例。中位年龄55岁（四分位距36-64岁）。其中肾肿瘤2例，肾上腺肿瘤1例，腹膜后肿瘤1例，... 详细信息

目的探讨三维影像重建在泌尿外科机器人手术中的应用价值。方法回顾性分析2017年9月至2018年6月收治的7例泌尿系肿瘤患者的病例资料。男4例，女3例。中位年龄55岁（四分位距36-64岁）。其中肾肿瘤2例，肾上腺肿瘤1例，腹膜后肿瘤1例，肾癌术后转移1例，膀胱神经内分泌肿瘤1例，脐尿管肿瘤1例。7例均行CT四期增强扫描，采集CT检查数据通过3D分析规划系统工作站重建成三维影像模型，以辅助手术方案规划和术中定位。借助三维影像模型，观察肿瘤大小、位置，以及肿瘤与血管及周围组织器官的关系等。7例均行机器人辅助腹腔镜手术，2例行肾部分切除术（其中1例为经后腹膜腔无肾动脉控制肾部分切除术），1例行有肾上腺肿瘤切除术，1例行经腹腔左肾门异位嗜铬细胞瘤切除术，1例行经腹腔右肾上腺和腔静脉后转移肿瘤切除术，1例行经腹腔根治性膀胱切除＋原位新膀胱＋扩大盆腔淋巴结清扫术，1例行经腹腔脐尿管肿瘤切除＋膀胱部分切除＋盆腔淋巴结清扫术。结果CT图像经三维重建后，清晰显示了立体的肿瘤位置以及与血管及周围组织器官的关系。7例手术均顺利完成，无中转开放手术。中位手术时问225min（四分位距135-280min），中位出血量50ml（四分位距20-600m1）。术后1例出现发热，7例围手术期均未发生Clavien DindoⅡ级以上并发症。结论三维影像重建技术在泌尿外科机器人手术方面具有实用价值，在选择手术入路、减少术中组织和器官损伤、降低相关手术并发症和提高手术成功率方面有重要的帮助。

关键词：三维影像重建泌尿外科机器人手术安全性临床使用价值