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A facile strategy for tuning the density of surface-grafted biom.lecules for m.lt extrusion-based additive m.nufacturing applications

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Bio-Design and m.nufacturing 2024年第3期7卷 277-291页

作者： I.A.O.Beeren G.Dos Santos P.j.Dij.stra C.m.ta j.Bauer H.Ferreira Rui L.Reis N.Neves S.Cam.rero-espinosa m.B.Baker L.m.roni Departm.nt of Com.lex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal ICVS/3B’s-PT Governm.nt Associate Laboratory 4806-909 Braga/GuimarãesPortugal POLYm.T University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain IKERBASQUE Basque Foundation for Science48009 BilbaoSpain

m.lt extrusion-based additive m.nufacturing(m.-Am.is a prom.sing technique to fabricate porous scaffolds for tissue engi-neering ***,m.st synthetic sem.crystalline polym.rs do not possess the intrinsic biological activity required to control cell *** of biom.lecules on polym.ric surfaces of Am.scaffolds enhances the bioactivity of a construct;however,there are lim.ted strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low m.lecular weight poly(ε-caprolactone)5k(PCL5k)containing orthogonally reactive azide groups with an unfunctionalized high m.lecular weight PCL75k at different *** porous three-dim.nsional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low m.lecular weight PCL *** a proof-of-concept test,we prepared film. of three different m.ss ratios of low and high m.lecular weight polym.rs with a therm.press and reacted with an alkynated fluorescent m.del com.ound on the surface,yielding a density of 201-561 pm.l/cm.(2).Subsequently,a bone m.rphogenetic protein 2(Bm.-2)-derived peptide was grafted onto the film. com.rising different blend com.ositions,and the effect of peptide surface density on the osteogenic differentiation of hum.n m.senchym.l strom.l cells(hm.Cs)was *** two weeks of culturing in a basic m.dium.cells expressed higher levels of Bm. receptor II(Bm.RII)on film. with the conj.gated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on film. contain-ing the highest peptide density(i.e.,561 pm.l/cm.(2)),indicating the im.ortance of the surface *** together,these results em.hasize that the density of surface peptides on cell differentiation m.st be considered at the cell-m.terial ***,we have presented a viable strategy for m.-Am.com.unity that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive m.nufacturing Blending Surface functionalization Surface density Click chem.stry Hum.n

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Exploring the effects of taurolidine on tum.r weight and m.crovessel density in a m.rine m.del of osteosarcom.

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Oncology Research 2024年第7期32卷 1163-1172页

作者： LISANNE K.A.NEIj.NHUIS LEUTA L.NAUm.NN SONIA A.m.FERKEL SAm.EL j.S.RUBIN STEPHAN ROGALLA Division of Gastroenterology and Hepatology Department of MedicineSchool of MedicineStanford UniversityStanford94305USA Departm.nt of Surgery Leiden University Medical CenterLeiden2333 ZAThe Netherlands Centre for Hum.n Drug Research Leiden2333 CLThe Netherlands Departm.nt of General VisceralVascular and Thoracic SurgeryCharite University Medicine BerlinBerlin10117Germany

Background:Osteosarcom. is the m.st com.on m.lignant prim.ry bone *** prognosis for patients with dissem.nated disease rem.ins very poor despite recent advancem.nts in ***,current treatm.nt regim.ns bear a signiﬁcant risk of serious side ***,there is an unm.t clinical need for effective therapies with im.roved safety proﬁ*** is an antibacterial agent that has been shown to induce cell death in different types of cancer cell ***:In this study,we exam.ned both the antineoplastic and antiangiogenic effects of taurolidine in anim.l m.dels of osteosarcom..K7m. m.rine osteosarcom. cells were inj.cted,both intram.scular and intraperitoneal,into 60 BALB/c m.ce on day *** were then random.zed to receive treatm.nt with taurolidine 2%(800 m./kg),taurolidine 1%(400 m./kg),or NaCl 0.9%control for seven days by intravenous or intraperitoneal ***:After 35 days,m.ce were euthanized,and the tum.rs were harvested for *** m.ce were excluded from.the analysis due to *** weight was signiﬁcantly lower in the 2%taurolidine intraperitoneal treatm.nt group from.day 9 to 21,consistent with elevated m.rtality in this *** tum.r weight was signiﬁcantly lower in the 1%(p=0.003)and 2%(p=0.006)intraperitoneal taurolidine treatm.nt groups com.ared to the *** antineoplastic effects were observed on intram.scular tum.rs or for intravenous adm.nistration of *** were no signiﬁcant differences in m.crovessel density or m.totic rate between treatm.nt *** body weight and elevated m.rtality in the 2%taurolidine intraperitoneal group suggest that the lower 1%dose is ***:In conclusion,there is no evidence of antiangiogenic activity,and the antitum.r effects of taurolidine on osteosarcom. observed in this study are ***,its toxic proﬁle grants further *** these observations,further research is necessary to

关键词： Osteosarcom. Taurolidine Cancer treatm.nt Chem.therapy m.rine m.dels

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Energy and blast perform.nce of beryllium.in a m.del therm.baric com.osition in com.arison with alum.num.and m.gnesium

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Defence Technology（防务技术） 2024年第6期36卷 13-19页

作者： Thom.s m.Klapotke Stanisław Cudziło Waldem.r A.Trzcinski j.zef Paszula Energetic m.terials Research Department of ChemistryUniversity of Munich(LMU)Butenandtstrasse 5-13(D)81377MunichGermany m.litary University of Technology Faculty of Advanced Technologies and ChemistrySylwester Kaliski Str.200-908WarsawPoland

A direct com.arison is m.de between the effectiveness of Al,m.,and Be powders as additional fuels in m.del therm.baric com.ositions containing 20%fuel,20%am.onium.perchlorate,and 60%RDX(1,3,5-Trinitro-1,3,5-triazacyclohexane)passivated with *** determ.ned calorim.tric m.asurem.nts of the heat of detonation,along with the overpressure histories in an explosion cham.er filled with nitrogen,were used to determ.ne the quasi-static pressure(QSP)under anaerobic *** m.asurem.nts were also perform.d in a sem.-closed bunker,and all blast wave param.ters generated after the detonation of 500 g charges of the tested explosives were *** calorim.try results,QSP values,and blast wave param.ters(pressure am.litude,specific and total im.ulses)clearly indicate that Be is m.ch m.re effective as an additional fuel than either Al or m. in both anaerobic post-detonation reactions as well as the subsequent aerobic *** heat of detonation of the RDXwax/AP/Be explosive m.xture is over 40%and 50%higher than that of the m.xture containing alum.num.and m.gnesium.instead of beryllium.***,the TNT equivalent of the Be-containing com.osition due to the overpressure in the nitrogen-filled explosion cham.er is 1.66,while the equivalent calculated using an air shock wave-specific im.ulse at a distance of 2.5 m.is equal to *** high values of these param.ters confirm.the high reactivity of beryllium.in both the anaerobic and aerobic stages of the therm.baric explosion.

关键词： Therm.baric explosives Beryllium Heat of detonation Quasi-static overpressure Blast wave param.ters

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Finding biom.rkers of experience in anim.ls

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j.urnal of Anim.l Science and Biotechnology 2024年第3期15卷 925-944页

作者： Sarah Babington Alan j.Tilbrook Shane K.m.loney j.ll N.Fernandes Tam.yn m.Crowley Luoyang Ding Archa H.Fox Song Zhang Elise A.Kho Daniel Cozzolino Tim.thy j.m.hony Dom.nique Blache School of Agriculture and Environm.nt The University of Western AustraliaCrawleyWA 6009Australia Centre for Anim.l Science The Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt LuciaQLD 4072Australia School of Veterinary Science The University of QueenslandGattonQLD 4343Australia School of Hum.n Sciences The University of Western AustraliaCrawleyWA 6009Australia School of m.dicine Deakin UniversityGeelongVIC 3217Australia Poultry Hub Australia University of New EnglandArmidaleNSW 2350Australia College of Anim.l Science and Technology Yangzhou UniversityYangzhou 225009China Centre for Nutrition and Food Sciences The Queensland Alliance for Agriculture and Food InnovationThe University of QueenslandSt LuciaQLD 4072Australia

At a tim. when there is a growing public interest in anim.l welfare,it is critical to have obj.ctive m.ans to assess the way that an anim.l experiences a *** is critical to ensure appropriate anim.l welfare *** behavioural,physiological,and neurobiological indicators that are used to assess anim.l welfare can verify the absence of extrem.ly negative *** welfare is m.re than an absence of negative outcom.s and an appropriate indicator should reflect the full spectrum.of experience of an anim.l,from.negative to *** this review,we draw from.the knowledge of hum.n biom.dical science to propose a list of candidate biological m.rkers(biom.rkers)that should reflect the experiential state of non-hum.n *** proposed biom.rkers can be classified on their m.in function as endocrine,oxidative stress,non-coding m.lecular,and therm.biological *** also discuss practical challenges that m.st be addressed before any of these biom.rkers can becom. useful to assess the experience of an anim.l in real-life.

关键词： Anim.l experience Anim.l welfare Biom.rker Stress Welfare assessm.nt

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m.d-term.survival of the Optim.s short stem. A prospective case series of 500 patients

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World j.urnal of Orthopedics 2024年第3期15卷 257-265页

作者： Bryan Ham.ns Sheryl de Waard Sj.erd Kaarsem.ker esther R C j.nssen Inger N Sierevelt Gino m.m.j.Kerkhoffs Daniël Haverkam. Departm.nt of Orthopaedic Surgery Xpert ClinicAmsterdam 1101EANetherlands Departm.nt of Orthopedic Surgery Amsterdam University Medical CenterAmsterdam 1105 AZNetherlands esther R C j.nssen Department of Orthopaedic SurgeryViecuri Medisch CentrumVenlo 5912 BLNetherlands Centre for Orthopaedic Research Spaarne ZiekenhuisHoofddorp 2134 TMNetherlands Departm.nt of Orthopaedic Surgery and Sports m.dicine Amsterdam UMC-Location AMCAmsterdam 1105 AZNetherlands Departm.nt of Orthopedic Surgery Xpert Orthopedic Surgery ClinicAmsterdam 1101 EANetherlands

BACKGROUND In recent years,there has been an increase in the num.er of total hip arthroplasty procedures in the younger patient *** active group has higher expectations of their prosthesis in com.arison to the older population,and there is a greater physical dem.nd for the *** fem.ral stem. were in-troduced to retain proxim.l bone stock and j.int biom.chanics and becam. m.re com.on to im.lant in this specific ***,the long-term.survival and functional outcom.s of various short stem. are still being investigated in different *** To determ.ne the 5-year survival of the Optim.s hip *** This was a prospective m.lticenter cohort study of 500 patients conducted in two hospitals in the *** patients received the Optim.s short stem.m.thys Ltd,Bettlach,Switzerland).The prim.ry outcom. m.asure was survival of the hip stem.with revision as the *** secondary outcom. m.asurem.nts included patient-reported outcom. m.asures(PROm.).Kaplan-m.ier analysis was used to calculate the 5-year survival ***-m.nus-log transform.tion was perform.d to calculate the 95%confidence interval(95%CI).m.xed m.del analyses were perform.d to assess the course of the PROm. during the 1st 2 years after *** were m.deled separately for the 1st and 2nd years to calculate the yearly change in PROm. during both follow-up periods with accom.anying 95%*** The m.an age of the total 500 patients was 62.3 years(standard deviation:10.6)and 202 were m.le(40%).At a m.dian follow-up of 5.5 years(interquartile range:4.5-6.7),7 patients were deceased and 6 revisions were registered,for infection(n=3),subsidence(n=2)and m.lposition(n=1).This resulted in an overall 5-year survival of 98.8%(95%CI:97.3-99.5).If infection was left out as reason for revision,a stem.survival of 99.4%(95%CI:98.1-99.8)was *** questionnaires were com.leted by 471 patients(94%),317 patients(63%)com.leted the 1-year follow-up questionna

关键词： Total hip arthroplasty Fem.ral stem Short stem Optim.s Survival 5-year survival Revision PROm.

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Surgeon preferences in the treatm.nt of thum. carpom.tacarpal osteoarthritis

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World j.urnal of Orthopedics 2024年第5期15卷 435-443页

作者： Edward j.Wu Bradley W Fossum.Wyatt Vander Voort Christopher O Bayne Robert m.Szabo Departm.nt of Orthopedic Surgery University of Minnesota Medical SchoolMinneapolisMN 55455United States Departm.nt of Orthopaedic Surgery UC Davis School of MedicineSacramentoCA 95817United States

BACKGROUND Prim.ry thum. carpom.tacarpal(Cm.)osteoarthritis is one of the m.st com.on conditions encountered by hand *** the vast num.er of operations that have been proposed,none have dem.nstrated results significantly superior to trapeziectom. *** The purpose of our study was to determ.ne why surgeons opt for their technique in treating Cm. *** A cross-sectional survey of active m.m.ers of the Am.rican Society for Surgery of the Hand was conducted to evaluate the reasons behind their preferred technique in the treatm.nt of isolated thum. Cm. *** were contacted by em.il once and provided with a link to a de-identified survey consisting of 5 treatm.nt questions and 5 dem.graphic *** Of 950 responses were received.40.5%of surgeons preferred trapeziectom.+ligam.nt reconstruction tendon interposition(LRTI),followed by trapeziectom.+suspensionplasty(28.2%),suture button suspension(5.9%),trapeziectom. alone(4.6%),prosthetic arthroplasty(3.2%),arthrodesis(1.1%),and other(6.6%).Proponents of trapeziectom.+LRTI cited fam.liarity(73.2%),exposure during fellowship(48.8%)and less proxim.l m.gration(60%)to be the m.in reasons affecting their *** who preferred trapeziectom.+suspensionplasty m.st reported simplicity(74.9%),fewer com.lications(45.3%),less proxim.l m.gration(43.8%),and avoidance of autogenous tissue harvest(42.7%).Advocates of suture button suspension cited avoidance of autogenous tissue harvest(80.4%),shorter im.obilization(76.8%),and quicker recovery(73.2%)with their *** of trapeziectom. alone cited simplicity(97.7%),fewer com.lications(86.4%),and avoidance of autogenous tissue harvest(59.1%).In their com.ents,45%of surgeons choosing trapeziectom. alone cited evidence as an additional *** of prosthetic arthroplasty cited im.roved pinch strength(83.3%)and im.roved range of m.tion(63.3%),while those preferring arthrodesis cited better pinch strength(90%)and freque

关键词： Thum. carpom.tacarpal Osteoarthritis Trapeziectom. Ligam.nt reconstruction tendon interposition Suspensionplasty Preferences Trends

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The Iberian Rom. Population Variant Server(IRPVS)

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j.urnal of Genetics and Genom.cs 2024年第7期51卷 769-773页

作者： Fabiola m.villard j.vier Perez-Florido francisco m.Ortuño Am.dor Valladares m.ren L.Álvarez-Villegas Gem. Roldán Rosario Carm.na m.nuel Soriano Santiago Susarte Pilar Fuentes Daniel López-López Ana m.ría Nuñez-Negrillo Alej.ndra Carvaj.l Yolanda m.rgado Daniel Arteaga Rosa Ufano Pablo m.r j.an F.Gam.lla j.aquín Dopazo Carm.n Paradas m.carena Cabrera-Serrano Instituto de Biom.dicina de Sevilla(IBiS) Hospital Universitario Virgen del Rocío/CSIC/Universidad de SevillaSevillaSpain Centro Investigación Biom.dica en Red Enferm.dades Neurodegenerativas(CIBERNED) Instituto de Salud CarlosⅢSevillaSpain Plataform. Andaluza de m.dicina Com.utacional Fundación Progreso y Salud(FPS)Hospital Virgen del RocíoSevillaSpain Grupo de m.dicina com.utacional de sistem.s Instituto de Biomedicina de Sevilla(IBiS)Hospital Virgen del RocíoSevillaSpain Nodo de Genóm.ca Funcional (INB-ELIXIR-es)Fundación Progreso y Salud(FPS)Hospital Virgen del RocíoSevilla 41013Spain Bioinform.tica en Enferm.dades raras(BiER) Centro de Investigación Biomédica en Red de Enfermedades Raras(CIBERER)Instituto de salud CarlosⅢSevillaSpain Departam.nto de Ingeniería de Com.utadores Automática y RobóticaUniversidad de GranadaGranadaSpain Servicio de Urgencias de Atención Prim.ria Distrito SevillaSevillaSpain Centro de Servicios Sociales Negociado de Servicios EspecializadosAyuntamiento de SevillaSevillaSpain Departam.nto de Enferm.ría Facultad de Ciencias de la SaludUniversidad de GranadaGranadaSpain Departam.nto de Neurología Hospital Virgen de las NievesGranadaSpain Departam.nto de Neurología Hospital Virgen de ValmeSevillaSpain Centro de Salud Utrera Sur SevillaSpain Centro de Salud Polígono Sur SevillaSpain Unidad de Trastornos del m.vim.ento Servicio de Neurología y Neurofisiología ClínicaHospital Universitario Virgen del RocíoSevillaSpain Departam.nto de m.dicina Facultad de MedicinaUniversidad de SevillaSevilleSpain Departam.nto de Antropología Social Universidad de GranadaSpain Unidad Enferm.dades Neurom.sculares Servicio de Neurología y Neurofisiología ClínicaHospital Universitario Virgen del RocíoSevillaSpain

In recent years,the num.er of known disease genes has exponentially grown due to the widespread adoption of cost-effective m.ssive parallel *** identify new disease variants,especially in m.nogenic disorders,a frequen... 详细信息

关键词： Server filtering m.ssive

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Phase com.osition,conductivity,and sensor properties of cerium.doped indium.oxide

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Nano m.terials Science 2024年第2期6卷 193-200页

作者： m.I.Ikim.G.N.Gerasimov V.F.Grom.v O.j.Ilegbusi L.I.Trakhtenberg N.N.Sem.nov Federal Research Center for Chem.cal Physics of RAS MoscowRussia University of Central Florida OrlandoFLUSA m.scow Institute of Physics and Technology(State University) DolgoprudnyMoscow RegionRussia Lom.nosov m.scow State University MoscowRussia

The hydrotherm.l synthesis of In_(2)O_(3)and CeO_(2)–In_(2)O_(3)is investigated as well as the properties of sensor layers based on these *** the synthesis of In_(2)O_(3),interm.diate products In(OH)_(3)and InOOH are form.d,which are the precursors of stable cubic(c-In_(2)O_(3))and m.tastable rhom.ohedral(rh-In_(2)O_(3))phases,respectively.A transition from.c-In_(2)O_(3)to rh-In_(2)O_(3)is observed with the addition of CeO_(2).The introduction of cerium.into rh-In_(2)O_(3)results in a decrease in the sensor response to hydrogen,while it increases in com.osites based on c-In_(2)O_(3).The data on the sensor activity of the com.osites correlate with XPS results in which CeO_(2)causes a decrease in the concentrations of chem.sorbed oxygen and oxygen vacancies in rh-In_(2)O_(3).The reverse situation is observed in com.osites based on c-In_(2)O_(3).Com.ared to In_(2)O_(3)and CeO_(2)–In_(2)O_(3)obtained by other m.thods,the synthesized com.osites dem.nstrate m.xim.m.response to H_(2)at low tem.eratures by 70–100℃,and have short response tim.(0.2–0.5 s),short recovery tim.(6–7 s),and long-term.stability.A m.del is proposed for the dependence of sensitivity on the direction of electron transfer between In_(2)O_(3)and CeO_(2).

关键词： Cerium.oxide Indium.oxide Nanocom.osite Hydrotherm.l m.thod Cubic phase Rhom.ohedral phase Sensor response Conductivity Hydrogen Response/recovery tim.

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Com.ressive property and energy absorption characteristic of interconnected porous m.-Zn-Y alloys with adj.sting Y addition

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j.urnal of m.gnesium.and Alloys 2024年第1期12卷 171-185页

作者： j.A.Liu S.j.Liu B.Wang W.B.Sun X.j.Liu Z.W.Han College of m.terials Science and Engineering Key Laboratory of Automobile Materials(Ministry of Education)Jilin UniversityChangchun 130022PR China Key Laboratory of Bionic Engineering(m.nistry of Education) College of Biological and Agricultural EngineeringJilin UniversityChangchun130022PR China Weihai Institute for Bionics-j.lin University Weihai 264205PR China

In this study,interconnected porous m.-2Zn-xY alloys with different phase com.ositions were prepared by various Y additions(x=0.4,3,and 6 wt.%)to adj.st the com.ressive properties and energy absorption *** characterization m.thods were then applied to identify the m.crostructure of the porous m.-Zn-Y and describe the details of the second *** tests were perform.d at room.tem.erature(RT),200℃,and 300℃to study the im.act of the Y addition and testing tem.erature on the com.ressive properties of the porous *** experim.ntal results showed that a high Y content prom.tes a m.crostructure refinem.nt and increases the volum. fraction of the second *** the Y content increases,different m.-Zn-Y ternary phases appear:I-phase(m._(3)Zn_(6)Y),W-phase(m._(3)Zn_(3)Y_(2)),and LPSO phase(m._(12)ZnY).When the Y content ranges between 0.4%and 6%,the com.ressive strength increases from.6.30m.a to 9.23 m.a,and the energy absorption capacity increases from.7.33 m.0}m.(3)to 10.97 m.0}m.(3)at RT,which is m.inly attributed to the phase com.osition and volum. fraction of the second ***,the average energy absorption efficiency is independent of the Y *** addition,the com.ressive deform.tion behaviors of the porous m.-Zn-Y are altered by the testing *** com.ressive strength and energy absorption capacity of the porous m.-Zn-Y decrease due to the softening effect of the high tem.erature on the *** deform.tion behaviors at different tem.eratures are finally observed to reflect the failure m.chanism. of the struts.

关键词： Porous m.gnesium Rare earth elem.nts m.crostructure Com.ressive behavior Energy absorption characteristic

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m.ltivariate form.of Herm.te sam.ling series

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Applied m.them.tics(A j.urnal of Chinese Universities) 2024年第2期39卷 253-265页

作者： Rashad m.Asharabi Departm.nt of m.them.tics College of Arts and SciencesNajran UniversityNajranSaudi Arabia

In this paper,we establish a new m.ltivariate Herm.te sam.ling series involving sam.les from.the function itself and its m.xed and non-m.xed partial derivatives of arbitrary *** m.ltivariate form.of Herm.te sam.ling will be valid for som. classes of m.ltivariate entire functions,satisfying certain growth *** will show that m.ny known results included in Com.un Korean m.th Soc,2002,17:731-740,Turk j.m.th,2017,41:387-403 and Filom.t,2020,34:3339-3347 are special cases of our ***,we estim.te the truncation error of this sam.ling based on localized sam.ling without decay *** exam.les are also presented.

关键词： m.ltidim.nsional sam.ling series sam.ling with partial derivatives contour integral truncation error

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