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Mollusk Species Richness on the Rocky Shores of the State of Guerrero, Mexico, as Affected by Rains and Their Geographical Distribution

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Natural Resources 2012年第4期3卷 248-260页

作者： flores-rodríguez pedro flores-Garza Rafael García-Ibánez Sergio Valdés-González Arcadio Violante-González Juan Santiago Cortés Enedina Galeana-Rebolledo Lizeth Torreblanca-Ramírez Carmina Unidad Académica de Ecología Marina Universidad Autónoma de Guerrero Acapulco México Universidad Autónoma de Nuevo León Laboratorio de Acuacultura Facultad de Ciencias Biológicas Ciudad Universitaria San Nicolás de los Garza México

Natural processes in ecosystems shows significant variations in species richness with regular cycles that can be altered by natural or anthropogenic activities;the present research was in order to understand on some of these variations. The objectives were: 1) determine the species richness of rocky intertidal mollusks;2) track their geographical distribution at State level;and 3) determine the changes in species richness as a result of rainfall on an annual cycle. For these purposes it was sampled in March, June, September and December 2005, the sampled area was 20 m2for each collecting site;the sampling unit was a PVC rectangle frame one by two meter per side. The mollusks found within the sampling units were collected, identified and counted. The species distribution was analyzed by sites and regions, considered species richness per season, and the distributional patterns by rainy season historical records. A total of 62 mollusks species were found, its richness associated with substrate stability and wave intensity on each site, Gastropods had the highest species richness. When analyzing the regional distribution and sites, it was found a pattern of species richness roughly constant in the proportion of species with wide distribution (generalists) versus those of restricted spatial representation (specialists). Gastropods had the highest number of species with restricted spatial representation. Seasonal rainfall differences did not quantitatively altered on the overall the species of mollusks in the rocky intertidal sampled zone however, in the analysis per Class, Gastropoda indicated changes in species richness influenced by rainfall, which were not observed in Bivalvia and Polyplacophora, suggesting that those changes might depend on adaptive processes or the lack of adaptation of gastropod species to the characteristics of the rocky intertidal habitat.

关键词： Rocky Shore Species Richness Geographic Distribution Rain Effects Mollusks

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Effects of Imazethapyr-Based Herbicide Formulation in the Zebrafish (Danio rerio) Hepatocyte Cell Line (ZF-L): Cytotoxicity and Oxidative Stress

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Journal of Environmental Protection 2024年第6期15卷 747-765页

作者： Edila Maria Kickhöfel Ferrer Milena Mattes Cerveira pedro Henrique flores Da Cruz Cleiton Jesus Andrade Pereira Lucas Moraes Berneira Claudio Martin Pereira de Pereira Nathalia Stark Pedra Roselia Maria Spanevello Jean Carlos Bauer Vieira Clarissa Piccinin Frizzo Mateus Tavares Kütter Janice Luehring Giongo rodrigo de Almeida Vaucher Center of Chemical Pharmaceutical and Food Sciences Microorganism Biochemistry and Molecular Biology Research Laboratory (LAPEBBIOM) Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Center of Chemical Pharmaceutical and Food Sciences Laboratory for Lipidomic and Bio-Organic Research Bioforensic Research Group Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Center of Chemical Pharmaceutical and Food Sciences Neurochemistry Inflammation and Cancer Laboratory Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Department of Chemistry Heterocycle Chemistry Center (NUQUIMHE) Federal University of Santa Maria (UFSM) Santa Maria Rio Grande do Sul Brazil Department of Microbiology and Parasitology Biology Institute Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil

Seizures of agrochemical formulations have increased in Brazil and Rio Grande do Sul is among the Brazilian states with the highest number of seizures of these products obtained illicitly. The use of illicit formulations can cause significant harm to agricultural production, the environment, and non-target species. This study evaluated the cytotoxicity and oxidative stress of a seized formulation containing the herbicide imazethapyr (IMZT). Characterization of the herbicide included gas chromatography-mass spectrometry (GC-MS) and thermal analyses (thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC)). Hemolytic and cytotoxicity assays in ZF-L hepatic cells showed IC50 values of 12.75 µg/mL, 3.01 µg/mL, 2.67 µg/mL, and 1.61 µg/mL for erythrocytes, [3(4,5-dimethyl)-2 bromide-5 diphenyl tetrazolium] (MTT), neutral red (NR), and lactate dehydrogenase (LDH) assays, respectively. The median IC50 of 2.84 µg/mL was used in oxidative stress assays, revealing increased reactive oxygen species (ROS) production, reduced total sulfhydryl content, and decreased superoxide dismutase (SOD) and catalase (CAT) activity. This study is the first to report in vitro oxidative stress induced by IMZT in the ZF-L cell line, emphasizing the importance of in vitro assays for assessing the toxic effects of seized agrochemicals on human health and the environment.

关键词： Agrochemical Imidazolinone Zebrafish Cell Viability Oxidative Damage

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Diversity, Distribution and Composition of the Bivalvia Class on the Rocky Intertidal Zone of Marine Priority Region 32, Mexico

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Open Journal of Ecology 2014年第15期4卷 961-973页

作者： Rafael flores-Garza Victor López-Rojas pedro flores-rodríguez Carmina Torreblanca Ramírez Unidad Académica de Ecología Marina Universidad Autónoma de Guerrero Acapulco Mexico Unidad de Ciencias de Desarrollo Regional Universidad Autónoma de Guerrero Acapulco Mexico

The Bivalvia Class, is widely represented in marine ecosystems and is economically important;this research focused on this class and was carried out by sampling the rocky intertidal zone of seven sites located in the Marina Region Priority No. 32, Guerrero, Mexico, where the National Commission for the Knowledge and Use of Biodiversity reported lack of knowledge on marine species living there. The objectives were to examine species richness associated with the rocky intertidal zone, determine how the community is composed by the representation of families based on species richness and abundance, know the distribution of the species, analyze the structure sizes of populations and estimate the diversity index. Sampling was conducted at seven sites;the sampling unit was one square meter and the sampling area was 10 m2. 32 species were identified. Four species are new records for the Mexican Pacific Transitional: one for the state of Guerrero and one for Marine Priority Region 32. Arcidae and Mytilidae Families were better represented in species richness and Chamidae Family was the best represented in abundance. Chama coralloides was considered to be the representative species of the area;Striostrea prismatica showed the greatest size. The diversity index was estimated (H' = 3.65 bits/individuals). Species richness is high and corresponds to that expected in a tropical area.

关键词： Bivalvia Distribution Intertidial Zone Guerrero Mexico

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Potential role of noise to improve intracortical microstimulation in tactile neuroprostheses

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Neural Regeneration Research 2021年第8期16卷 1533-1534页

作者： pedro Mabil Nayeli Huidobro Amira flores Elias Manjarrez Laboratory of Integrative Neurophysiology Instituto de FisiologíaBenemérita Universidad Autónoma de PueblaCPMéxico

The intracortical microstimulation(ICMS) from 40 to 100 μA is useful to elicit tactile sensations,which could be employed in neuroprostheses to control the robotic arms' ***,this electrical current applied for prolonged periods of time could damage the neuronal ***,there is a necessity to create new strategies for the practical use of ICMS in a safe intensity range for potential clinical applications in tetraplegic *** we describe crucial studies supporting the use of electrical and optical noise to guarantee a safe ICMS delivered through brain-machine interface technologies.

关键词： guarantee supporting sensation

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Mollusks of the Rocky Intertidal Zone at Three Sites in Oaxaca,Mexico

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Open Journal of Marine Science 2014年第4期4卷 326-337页

作者： pedro flores-rodríguez Rafael flores-Garza Sergio García-Ibánez Carmina Torreblanca-Ramírez Lizeth Galeana-Rebolledo Enedina Santiago-Cortes Laboratorio de Ecologia Costera y Sustentabilidad Unidad Academica de Ecologia MarinaUniversidad Autonoma de GuerreroAcapulcoMexico Doctorado en Ciencias Ambientales Unidad de Ciencias de Desarrollo RegionalUniversidad Autonoma de GuerreroAcapulcoMexico

The community of mollusks at three rocky intertidal zone in Oaxaca, Mexico was analyzed. Two samplings per site were conducted. At each sampling the inspected area was 10 m2. The sampling unit was 1 m2. 5862 specimens were examined. 68 mollusks species were identified. The families better represented in species richness were: FISSURELLIDAE, MURICIDAE, LOTIIDAE, MYTILIDAE, CHAMIDAE, ISCHNOCHITONIDAE y CHITONIDAE. The species that had the greatest density were Lottia discors, Siphonaria palmata, Lottia acutapex, Chormoytilus palliopunctatus, Brachidontes adamsianus, Chton articulatus e Ischnochiton muscarius. The species with the widest distribution represent 11.76%. With irregular distribution was found in 58.82% of the species. The gastropod Crucibulummonticulus, the bivalve C. palliopunctatus and the polyplacophoran C. articulatus had the greatest size. The value of diversity index was H’ = 4.29 bits/ind. and J’ = 0.72. The mollusks community in the rocky intertidal zone study sites is characterized by small size, because they rarely exceed seventy millimeters. The values of H’ and J’ indicate that the mollusk community in the study area has a high diversity and high uniformity, corresponding to mature and stable communities in a tropical region.

关键词： Intertidal Zone Oaxaca Biodiversity Mollusks

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Density,Sex Ratio,Size,Weight,and Recruitment of Plicopurpura pansa(Gastropoda:Muricidae)in Costa Chica,Guerrero,Mexico

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Open Journal of Marine Science 2012年第4期2卷 157-166页

作者： Rafael flores-Garza Arcadio Valdés González pedro flores-rodríguez Sergio García-Ibánez Norma L.Cruz-Ramirez Unidad Académica de Ecología Marina Universidad Autónoma de GuerreroFraccionamiento las PlayasAcapulcoMéxico Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo LeónSan Nicolas de los GarzaMéxico

Plicopurpura pansa is a cultural resource with economic importance since ink can be extracted from it to dye clothes. This snail inhabits the rocky intertidal zone of Mexican Pacific, is found attached to rocks in crevices and holes. The objective was to analyze the P. pansa population structure in Costa Chica of the State of Guerrero based in density, sex ratio, size and weight, besides to realize observations on mating and recruitment, for which quarterly sampling was made during an annual cycle in three beaches. The density was 4.83 snails/m2. Sex ratio ranged from 0.76:1.00 to 1.00: 0.96 (F:M). Average size was 20.91 mm. The best represented sizes in females were from 10.5 to 28.5 mm and in males 13.5 to 28.5 mm. Averaged weight was 1.99 g, females showed a coefficient of variation in weight of 96.53%. Recruitment occurred for the entire year. In December 2006 and September 2007 a larger frequency of recruits was shown. Sex ratio showed a 1:1 tendency. Snail population was characterized by being small sized and in high densities. Weight variations in females were found related to their reproductive cycle.

关键词： Gender Recruitment Intertidal Ink

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The Conidae Family (Snails Producers of Poisons) Associated with the Rocky Intertidal Zone of Acapulco, Mexico

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Natural Resources 2014年第8期5卷 343-350页

作者： Rafael flores-Garza Yareni M. Garcia-Moctezuma pedro flores-rodríguez Jesús E. Michel-Morfín Carmina Torreblanca-Ramírez Departamento de Estudios para el Desarrollo Sustentable de Zonas Costeras Universidad de Guadalajara Jalisco Mexico Laboratorio de Ecología Costera y Sustentabilidad Unidad Académica de Ecología Marina Universidad Autónoma de Guerrero Acapulco Mexico Unidad de Ciencias de Desarrollo Regional Universidad Autónoma de Guerrero Acapulco Mexico

Acapulco, Mexico has a coastline of 62 km where large areas of rocky substrate inhabited by organisms such as the GASTROPODA Class can be found. Conidae (Fleming, 1822) Family is among the members of this Class, which has been poorly studied in the Mexican Pacific, despite their biomedical potential. This research was carried out in the rocky intertidal of nine sites in Acapulco. The objectives were: 1) Prepare an inventory of species associated with rocky intertidal of Conidae family, 2) examine the composition of the family based on two criteria: a) species richness and b) abundance, 3) determine the geographical distribution and 4) analyze the size composition. The unit sampled was 1 m2 and the sampled area was 10 m2 persite. A total of 97 organisms were analyzed, corresponding to two subfamilies, five genera and five species. The Puncticuliinae (Tucker & Tenorio, 2009) subfamily had higher species richness, abundance and frequency of occurrence. The most abundant and most common species were Harmoniconus nux (Broderip, 1833) and Gladioconus gladiator (Broderip, 1833) both showed regular distribution. The size structure found for Conidae family is similar to what has been reported in other research. For the first time G. gladiator is being reported for the state of Guerrero.

关键词： Conidae Acapulco Poison Distribution Rocky Intertidal Zone

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New Records for GASTROPODA Class of Species Found in the Rocky Intertidal Zone of the Marine Priority Region 32,Guerrero,Mexico

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Open Journal of Marine Science 2014年第3期4卷 221-237页

作者： Carmina Torreblanca-Ramírez Rafael flores-Garza Jesús Emilio Michel-Morfin Jose Luis Rosas-Acevedo pedro flores-rodríguez Sergio García-Ibánez Unidad de Ciencias de Desarrollo Regional Universidad Autónoma de GuerreroAcapulcoMexico Laboratorio de Ecología Costera y Sustentabilidad Unidad Académica de Ecología MarinaUniversidad Autónoma de GuerreroAcapulcoMexico Departamento de Estudios para el Desarrollo Sustentable de Zonas Costeras Universidad de GuadalajaraMelaqueMexico

The GASTROPODA class, one of the best known marine environments, is formed by a large number of species and the shape of their shell structure varies greatly. These mollusks are distributed from the intertidal zone all the way to the abyssal zone, and there are also swimming and floating species. This research was conducted at seven sites, located in the Marina Priority Region 32 (MPR 32), located in the State of Guerrero, Mexico. The aim of this report is to document the scope of the geographic distribution of the 34 species of GASTROPODA class. 11,263 total specimens were analyzed. 108 species were identified, of which four species are new records for Mexico, 15 for the Transitional Mexican Pacific, 11 for the State of Guerrero and four for the MPR 32. Here we report a significant number of new records of species of class GASTROPODA found on the rocky intertidal zone of the MPR 32. The reporting of these new records, demonstrates the need to focus research efforts on the study of marine diversity, since knowledge in this respect is quite incomplete, especially regarding marine mollusks, a fact that has been reported by the National Commission for the Management and Use of Biodiversity.

关键词： GASTROPODA Biodiversity New Records Intertidal Zone and Guerrero

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Statins for primary cardiovascular prevention in the elderly

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Journal of Geriatric Cardiology 2015年第4期12卷 431-438页

作者： Juan pedro-Botet Elisenda Climent Juan J Chillarón Rocio Toro David Benaiges Juana A flores-Le Roux Lipid and Vascular Risk Unit Endocrinology department Hospital del Mar. Spain Department of Medicine School of Medicine. Cáldiz University Cádiz Spain

老人口全球正在增加，与题目 >；组成快成长的年龄组的 65 岁。而且，老脸心血管的疾病死亡和病态的最大的风险和负担。尽管老病人，特别地那些更旧的 >；75，很好没在评估类脂化合物阴沉的治疗的使随机化的临床的审判被... 详细信息

老人口全球正在增加，与题目 >；组成快成长的年龄组的 65 岁。而且，老脸心血管的疾病死亡和病态的最大的风险和负担。尽管老病人，特别地那些更旧的 >；75，很好没在评估类脂化合物阴沉的治疗的使随机化的临床的审判被代表，在更老的个人在主要预防支持 statin 治疗的使用的可得到的证据主要从亚群分析和 hoc 以后的数据被导出。在另一方面，老病人经常有要求并发的药的一个高数字的多重合作病态；这可以为药药相互作用增加风险，从而减少 statin 的潜在的好处治疗。这评论的目的是关于 statin 使用介绍相关文学在为他们的主要心血管的疾病老，与治疗的联系风险和好处。

关键词：心血管疾病他汀类药物中老年人预防药物相互作用药物治疗年龄组世界范围

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Cycloheximide prevents production of arresting,a fraction of 30-50 kDa obtained from seminiferous tubule conditioned medium

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Asian Journal of Andrology 2004年第4期6卷 359-364页

作者： Gustavo F.Gonzales Stella Hartinger Yetssy flores Kelly Zárate pedro Yi Instituto de Investigations de la Altura Department of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesFaculty of Veterinary Medicine and Animal Sciences Department of Biological and Physiological Sciences and and and and Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima Peru

Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult rats cultured in vitro with or without cycloheximide to condition culture media, which have been concentrated, size fractioned (30-50 kDa) and administered 7 days to adult rats by intraperitoneal injections. The effects on testicular and epididymal weights, spermatogenesis and epididymal sperm count were determined. Results: The fraction (30-50 kDa), named arresting, obtained from the culture without cycloheximide decreased testicular and epididymal weights (P<0.01) and reduced the epididymal sperm count significantly. Study of the spermatogenic cycle by transillumination showed spermatogenic arrest at stage VII in rats treated with arresting compared to that observed in controls. The length of stage VII in the group receiving the seminiferous tubules culture media with cycloheximide (30-50 KDa CHX-STCM fraction) was similar to control. Conclusion: The difference in the effect may be the result of the presence or absence of arresting, a protein secreted by the tubules.

关键词： cycloheximide arresting conditioned medium seminiferous tubule sperm production transillumination

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