Cycloheximide prevents production of arresting,a fraction of 30-50 kDa obtained from seminiferous tubule conditioned medium

作     者：Gustavo F.Gonzales Stella Hartinger Yetssy Flores Kelly Zárate Pedro Yi GUSTAVO F. GONZALES;STELLA HARTINGER;YETSSY FLORES;KELLY ZARATE;PEDRO YIINSTITUTO DE INVESTIGACIONES DE LA ALTURA;DEPARTMENT OF BIOLOGICAL AND PHYSIOLOGICAL SCIENCES;AND FACULTY OF VETERINARY MEDICINE AND ANIMAL SCIENCES;UNIVERSIDAD PERUANA CAYETANO HEREDIA. POSTAL OFFICE . LIMA;PERU

作者机构：Instituto de Investigations de la AlturaDepartment of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesDepartment of Biological and Physiological SciencesFaculty of Veterinary Medicine and Animal Sciences Department of Biological and Physiological Sciences and and and and Universidad Peruana Cayetano Heredia. Postal Office 1843. Lima Peru 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2004年第6卷第4期

页      面：359-364页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 071008[理学-发育生物学] 

主　　题：cycloheximide arresting conditioned medium seminiferous tubule sperm production transillumination 

摘      要：Aim: To evaluate the effect of a protein synthesis inhibitor cycloheximide on arresting activity in spermato-genesis and sperm count in male rats. Methods: The study used seminiferous tubule (ST) segments from adult rats cultured in vitro with or without cycloheximide to condition culture media, which have been concentrated, size fractioned (30-50 kDa) and administered 7 days to adult rats by intraperitoneal injections. The effects on testicular and epididymal weights, spermatogenesis and epididymal sperm count were determined. Results: The fraction (30-50 kDa), named arresting, obtained from the culture without cycloheximide decreased testicular and epididymal weights (P0.01) and reduced the epididymal sperm count significantly. Study of the spermatogenic cycle by transillumination showed spermatogenic arrest at stage VII in rats treated with arresting compared to that observed in controls. The length of stage VII in the group receiving the seminiferous tubules culture media with cycloheximide (30-50 KDa CHX-STCM fraction) was similar to control. Conclusion: The difference in the effect may be the result of the presence or absence of arresting, a protein secreted by the tubules.