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Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains

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Open Journal of Animal Sciences 2020年第2期10卷 254-265页

作者： Sofia Lamas filipa Franquinho Marlene Morgado Fátima Gartner Irina Amorim Institute for Research and Innovation in Health (i3S) University of Porto Porto Portugal Animal Facility i3S/IBMC Porto Portugal Institute for Molecular and Cell Biology (IBMC) Porto Portugal Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP) Porto Portugal Department of Pathology and Molecular Immunology Institute of Biomedical Sciences Abel Salazar (ICBAS) University of Porto Porto Portugal

Superovulation is used to stimulate the production and release of large amounts of oocytes in mice by using two hormones that mimic FSH (PMSG) and LH (hCG) effects. Since superovulation can have a negative impact on oocyte and embryo development, this investigation aimed to compare two alternatives for 2-cells embryo collection in order to reduce the number of females and to benefit from the superovulation process. Data from mouse embryo collection from our facility was analyzed to compare the number of 2-cells embryos collected at 1.5 dpc and the number of 2-cells embryos obtained after overnight incubation of 1-cell embryos, collected at 0.5 dpc. Genetically modified mouse strains with a similar background (C57BL/6J, B6*129 and FVB/NJ) were analyzed and for strains at a C57BL/6J and B6*129 background, the number of 2-cells embryos obtained after incubation was significantly higher when compared to the number of 2-cells embryos collected at 1.5 dpc (1.4-fold and 1.7-fold, respectively). C57BL/6J wild type mice had similar results with a higher number of 2-cells embryos when collection was performed at 0.5 dpc followed by incubation (1.4-fold). These results can help the planning of 2-cells embryo harvesting by reducing the number of females needed for this procedure.

关键词： 0.5 Dpc Embryos 1.5 Dpc Embryos Incubation Superovulation

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Gastric cancer treated with pressurized intraperitoneal aerosol chemotherapy:revising an option for peritoneal carcinomatosis

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Journal of Cancer Metastasis and Treatment 2018年第1期4卷 104-109页

作者： filipa Macedo Katia Ladeira Adhemar Longatto-Filho Sandra F.Martins Portuguese Oncology Institute-Coimbra Coimbra 3000-075Portugal Portuguese Oncology Institute-Lisbon Lisbon 1099-023Portugal Life and Health Science Research Institute School of Health SciencesUniversity of MinhoBraga 4710-057Portugal ICVS/3B's-PT Government Associate Laboratory Braga/GuimaraesBraga 4710-057Portugal Molecular Oncology Research Center Barretos Cancer HospitalBarretosSao Paulo 14784-400Brazil Laboratory of Medical Investigation 14 Faculty of MedicineUniversity of Sao PauloSao Paulo 01246-903Brazil Surgery Department Coloproctology UnitBraga HospitalBraga 4710-243Portugal

Aim:Gastric cancer is the cancer with the highest rate of peritoneal metastization and this type of spread is associated with a higher death rate compared to dista nt orga n *** systemic chemotherapy has a mini mal effect in peritoneal metastasis so new types of treatment have *** authors revised the main studies done in pressurized in traperit on eal aerosol chemotherapy(PIPAC)and prese nted the main con clusi ***:A PubMed search was con ducted focus ing on PIPAC in gastric can *** MeSH database was searched with the terms:"Gastric can cer[MeSH]and in traperit on eal aerosol chemotherapy".Results:Seve n studies were an *** the studies performed the tech nique with aerosol of doxorubic in and cisplat *** cases were well tolerated,with minor adverse *** presented resolution of their abdominal symptoms and regression of macroscopic *** surgery or hypertermic intraperitoneal chemotherapy could be performed in some patients with good response to *** peritonitis caused by the chemotherapy was well ***:PIPAC can induce remission in end-stage and resistant disease with acceptable side effects,good safety levels for patients and health professionals,and quality of life improvement.

关键词： Pressurized intraperitoneal aerosol chemotherapy gastric cancer peritoneal carcinomatosis

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Progressive Multifocal Leukoencephalopathy—A Case Report in an Immunocompetent Patient

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Advances in Infectious Diseases 2018年第1期8卷 10-16页

作者： Ana Faceira Daniela Magalhaes filipa Ceia Joao Nuak Carina Reis Susana Ferreira Internal Medicine Department Hospital de Sao JoaoPortoPortugal Faculdade de Medicina da Universidade do Porto PortoPortugal Endocrinology Diabetes and Metabolism DepartmentHospital de Sao JoaoPortoPortugal Instituto de Investigacao e Inovacao em Saude Universidade do PortoPortoPortugal Infectious Diseases Department Hospital de Sao JoaoPortoPortugal Neuroradiology Department Hospital de Sao JoaoPortoPortugal

Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system due to the reactivation of the JC virus, which usually occurs in immunocompromised patients and is a major opportunistic infection associated with HIV infection. We report a case of a previously healthy patient who was diagnosed with PML.

关键词： Immunosuppression Immunocompetent JC Virus Progressive Multifocal Leukoencephalopathy

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Standardization of BIM Objects: Development of a Proposal for Portugal

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Open Journal of Civil Engineering 2016年第3期6卷 469-474页

作者： filipa Salvado Maria João Falcão Silva Paula Couto Álvaro Vale e Azevedo Buildings Department LNEC Lisbon Portugal

The management of information systems contributes to the economic development of the construction sector by organizing and structuring technical and economic information. It is proposed how BIM (Building Information Modelling) and a Portuguese information system-ProNIC (abbreviation for Protocol for the Standardization of Construction Technical Information) may be interconnected. The scope of this information transmission in construction sector is a step to solve several problems that have been identified in conception of BIM models.

关键词： BIM Objects Standardization Technical Information ProNIC

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Magnetic resonance imaging ancillary features used in Liver Imaging Reporting and Data System:An illustrative review

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World Journal of Radiology 2018年第2期10卷 9-23页

作者： David Campos-Correia Joao Cruz António P Matos filipa Figueiredo Miguel Ramalho Department of Radiology Centro Hospitalar de Lisboa OcidentalLisbon 1349-019Portugal Department of Radiology Hospital Garcia de OrtaAlmada 2805-267Portugal

Hepatocellular carcinoma (HCC) usually develops in the setting of chronic liver disease. In the adequate clinical context, both multiphasic contrast-enhanced CT and magnetic resonance imaging are non-invasive modalities that allow accurate diagnosis and staging of HCC, although the latter demonstrates greater sensitivity and specificity. Imaging criteria for HCC diagnosis rely on hemodynamic features such as hyperenhancement in the arterial phase and washout in the portal or equilibrium phase. However, imaging performance drops considerably for small (< 20 mm) nodules because their tendency to exhibit atypical enhancement patterns. In order to improve accuracy in the diagnosis and staging of HCC, particularly in cases of atypical nodules, ancillary features, i.e., imaging characteristics that modify the likelihood of HCC, have been described and incorporated into clinical reports, especially in Liver Imaging Reporting and Data System. In this paper, ancillary imaging features will be reviewed and illustrated.

关键词： Hepatocellular carcinoma Ancillary features Magnetic resonance imaging Liver Imaging Reporting and Data System Cirrhosis Liver

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Genotypic Assessment by RAPD Markers and Ultrastructural Characteristics of a NaCI-Tolerant Potato Cell Line

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Journal of Life Sciences 2012年第1期6卷 1-8页

作者： filipa Queiros Jose M. Almeida Domingos P.F. Almeida Femanda Fidalgo Biology Department Faculty of Science University of Porto Porto 4169-007 Portugal BioFIG-Center for Biodiversity Functional and Integrative Genomics Plant Molecular Biology ＆ Biotechnology Lab University of Porto Porto 4169-007 Portugal Faculty of Science University of Porto Porto 4169-007 Portugal CBQF College of Biotechnology Portuguese Catholic University Porto 4200-072 Portugal

Salinity is a serious threat to agricultural production. Potato （Solanum tuberosum） is an important food crop characterised for having low to moderate salinity tolerance. Tissue cultures may be relevant to improve salt tolerance in potato through selection of salt-tolerant cell lines and subsequent regeneration of plants. In this work, the authors used the random amplified polymorphic DNA （RAPD） markers to investigate the occurrence of genetic polymorphism in a potato calli line tolerant to 150 mM NaCI. Out of 40 primers screened, eight generated polymorphic patterns that distinguished salt-tolerant line from the control. Although the macroscopic appearance was similar in both lines, ultrastructural study revealed alterations in salt-grown cells. These showed that plastids less differentiated with a lower number of grana had more and larger starch grains than control cells. In conclusion, RAPD analysis revealed that NaCl-adapted line is a somaclonal variant and the ultrastructural study showed changes essentially at the plastids.

关键词： Salt tolerance potato callus tissue polymorphism molecular marker RAPD markers ultrastructure.

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Roadmap Proposal for Implementing Building Information Modelling (BIM) in Portugal

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Open Journal of Civil Engineering 2016年第3期6卷 475-481页

作者： Maria João Falcão Silva filipa Salvado Paula Couto Álvaro Vale e Azevedo Buildings Department LNEC Lisboa Portugal

Building Information Modeling (BIM) is a process involving generation and management of digital representations of physical and functional characteristics of places, which can be exchanged or networked to support decision-making in architecture, engineering and construction (AEC) sector. BIM is a relatively new technology in an industry typically slow to adopt changes, especially in Portugal. The present paper aims to frame the international implementation of BIM in order to propose a roadmap to spread the use of BIM methodologies in Portugal in the next decade.

关键词： BIM Roadmap International Implementation Strategies and Actions

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Effectiveness of Mesotherapy on Temporomandibular Joint Disorders

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Journal of Physical Science and Application 2015年第4期5卷 249-254页

作者： Andre Pedro Oliveira Cruz Ana filipa Rodrigues das Neves Isabel Ramires Manuel Mendonca Servico de Medicina Fisica e de Reabilitacao Hospital de S. Jose Centro Hospitalar de Lisboa Central Lisboa 1150-199 Portugal

TMJDs （Temporomandibular joint disorders） are a common pathology but best treatment remains unclear. The goal of this study was to evaluate the effectiveness of mesotherapy on TMJD. We conducted a case review of all TMJD patients treated since 2006 with mesotherapy procedures only and using a cocktail of drugs composing of lidocaine, piroxicam and pentoxifylline. Patients were treated every 15 days until symptomatic relief was achieved and thereafter every two months. Main outcome was complete symptomatic relief, and associated factors were analysed. 27 patients were included. Complete pain relief was achieved in 26. The necessary number of procedures to get pain relief ranged from 1 to 6 （median of 1） and the total number of treatments from 1 to 40 （median of 11）. 19 patients had other painful complains mainly related with anxiety disorders. A weak correlation （r = 0.357; p = 0.05） between anxiety disorders and the required months of treatments was found. Mesotherapy is a valuable option on pain relief in a temporomandibular pain syndrome with none of the systemic effects of oral medication. Anxiety may have a role on the aetiology and therapeutic success highlights the holistic approach of these patients. Future comparative studies are necessary.

关键词： TMJDs (Temporomandibular joint disorders) mesotherapy pain.

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Almond cold-pressed oil by-product as ingredient for cookies with potential health benefits:Chemical and sensory evaluation

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Food Science and Human Wellness 2019年第3期8卷 292-298页

作者： Joao C.M.Barreira M.Antonia Nunes Beatriz Vieira da Silva filipa B.Pimentel Anabela S.G.Costa M.Alvarez-Ortic J.E.Pardo M.Beatriz P.P.Oliveira REQUIMTE/LAQV Faculdade de Farmácia da Universidade do PortoR.Jorge Viterbo Ferreira 2284050-313PortoPortugal Centro de Investigacade Montanha(CIMO) Instituto Politécnico de BragancaCampus de Santa Apolónia5300-253PortoPortugal Escuela Técnica Superior de Ingenieros Agrónomos y de Montes Universidad de Castilla-La ManchaCampus Universitario02071AlbaceteSpain

Nowadays,food security is a global concern and a great scientific ***-industrial by-products can be used as potential ingredients of innovative food products,contributing to solve this societal ***-products of high specialty oils processing,for instance,have remarkable nutritional and organoleptic ***,the partially delipidified almond flour(PDAF)remaining from almond oil extraction,was used as an alternative ingredient in a highly appreciated almond-based cookie(“almendrados”).For comparison purposes,four different“almendrados”formulations(one industrial,two with PDAF,and one with whole almond flour),were compared regarding nutritional composition,vitamin E and fatty acids *** assess the cookie sensory properties,seventy-four consumers evaluated“appearance”,“taste”,“sweetness”,“crunchiness”,“hardness”,“overall quality”using adequate hedonic *** and protein levels showed significant differences among industrial and the other three *** with PDAF presented similar tocopherols and fatty acids to the traditional(more expensive)formulation,besides reaching the highest scores on“appearance”,“sweetness”,“hardness”,“overall quality”and“buying predisposition”***,the incorporation of PDAF might represent an effective way of adding value to an industrial by-product,while providing a pleasant novel cookie formulation.

关键词： By-products Almond Food security Cookie formulation Principal component analysis

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Giant cell arteritis: Current treatment and management

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World Journal of Clinical Cases 2015年第6期3卷 484-494页

作者： Cristina Ponte Ana filipa Rodrigues Lorraine O'Neill Raashid Ahmed Luqmani Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal Sciences Botnar Research Centre University of Oxford Oxford OX1 2JD United Kingdom Department of Rheumatology Hospital de Santa Maria CHLN Lisbon Academic Medical Centre 1649-035 Lisbon Portugal Department of Internal Medicine Hospital das Caldas da Rainha Centro Hospitalar Oeste 2500-176 Caldas da Rainha Portugal Department of Rheumatology St Vincent’s University Hospital Dublin Ireland

Glucocorticoids remain the cornerstone of medicaltherapy in giant cell arteritis(GCA) and should be started immediately to prevent severe consequences of the disease, such as blindness. However, glucocorticoid therapy leads to significant toxicity in over 80% of the patients. Various steroid-sparing agents have been tried, but robust scientific evidence of their efficacy and safety is still lacking. Tocilizumab, a monoclonal IL-6 receptor blocker, has shown promising results in a number of case series and is now being tested in a multi-centre randomized controlled trial. Other targeted treatments, such as the use of abatacept, are also now under investigation in GCA. The need for surgical treatment is rare and should ideally be performed in a quiescent phase of the disease. Not all patients follow the same course, but there are no valid biomarkers to assess therapy response. Monitoring of disease progress still relies on assessing clinical features and measuring inflammatory markers(C-reactive protein and erythrocyte sedimentation rate). Imaging techniques(e.g., ultrasound) are clearly important screening tools for aortic aneurysms and assessing patients with largevessel involvement, but may also have an important role as biomarkers of disease activity over time or in response to therapy. Although GCA is the most common form of primary vasculitis, the optimal strategies for treatment and monitoring remain uncertain.

关键词： Giant cell arteritis Therapy Disease management Glucocorticoids Immunosuppressive agents

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