Genotypic Assessment by RAPD Markers and Ultrastructural Characteristics of a NaCI-Tolerant Potato Cell Line
Genotypic Assessment by RAPD Markers and Ultrastructural Characteristics of a NaCI-Tolerant Potato Cell Line作者机构：Biology Department Faculty of Science University of Porto Porto 4169-007 Portugal BioFIG-Center for Biodiversity Functional and Integrative Genomics Plant Molecular Biology ＆ Biotechnology Lab University of Porto Porto 4169-007 Portugal Faculty of Science University of Porto Porto 4169-007 Portugal CBQF College of Biotechnology Portuguese Catholic University Porto 4200-072 Portugal
出 版 物：《Journal of Life Sciences》 (生命科学（英文版）)
年 卷 期：2012年第6卷第1期
页 面：1-8页
学科分类：09[农学] 0901[农学-作物学] 0902[农学-园艺学] 090201[农学-果树学]
主 题：Salt tolerance potato callus tissue polymorphism molecular marker RAPD markers ultrastructure.
摘 要：Salinity is a serious threat to agricultural production. Potato （Solanum tuberosum） is an important food crop characterised for having low to moderate salinity tolerance. Tissue cultures may be relevant to improve salt tolerance in potato through selection of salt-tolerant cell lines and subsequent regeneration of plants. In this work, the authors used the random amplified polymorphic DNA （RAPD） markers to investigate the occurrence of genetic polymorphism in a potato calli line tolerant to 150 mM NaCI. Out of 40 primers screened, eight generated polymorphic patterns that distinguished salt-tolerant line from the control. Although the macroscopic appearance was similar in both lines, ultrastructural study revealed alterations in salt-grown cells. These showed that plastids less differentiated with a lower number of grana had more and larger starch grains than control cells. In conclusion, RAPD analysis revealed that NaCl-adapted line is a somaclonal variant and the ultrastructural study showed changes essentially at the plastids.
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