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Incidence of urinary incontinence following endoscopic laser enucleation of the prostate by en-bloc and non-en-bloc techniques:a multicenter,real-world experience of 5068 patients

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Asian Journal of Andrology 2024年第3期26卷 233-238页

作者： Daniele Castellani Vineet Gauhar Khi Yung Fong Mario Sofer Moisés Rodríguez Socarrás Azimdjon N Tursunkulov Lie Kwok Ying Sarvajit Biligere Ho Yee Tiong Dean Elterman Abhay Mahajan Mark Taratkin Sorokin Nikolai Ivanovich Tanuj Pal Bhatia Dmitry Enikeev Nariman Gadzhiev Mohammed Taif Bendigeri Jeremy Yuen-Chun Teoh Marco Dellabella fernando Gómez Sancha Bhaskar Kumar Somani Thomas Reinhard William Herrmann Urology Unit IRCCS INRCAAncona 60127Italy Urology Unit Marche University HospitalLe Marche Polytechnic UniversityAncona 60126Italy Department of Urology Ng Teng Fong General HospitalSingapore 609606Singapore Yong Loo Lin School of Medicine National University of SingaporeSingapore 117597Singapore Department of Urology Tel Aviv Sourasky Medical CenterAffiliated to the Sackler School of MedicineTel Aviv UniversityTel Aviv 6423906Israel Department of Urology and Robotic Surgery ICUA-Clínica CEMTROMadrid 28701Spain Urology Division Akfa Medline HospitalTashkent 100211Uzbekistan Advanced Urology Gleneagles HospitalSingapore 258499Singapore Department of Urology National University HospitalSingapore 119228Singapore Divisionof Urology Department of SurgeryUniversity of TorontoOntarioToronto M5G 2C4Canada Department of Urology Mahatma Gandhi Mission’s Medical College and HospitalAurangabad 431003India Institute for Urology and Reproductive Health Sechenov UniversityMoscow119435Russian Federation Department of Urologyand Andrology Lomonosov Moscow State UniversityMoscow 119992Russian Federation Department of Urology Sarvodaya Hospital and Research CentreFaridabadHaryana 121006India Department of Urology Medical University of ViennaVienna 1090Austria Department of Urology Saint-Petersburg State University HospitalSaint-Petersburg 199034Russian Federation Department of Urology Asian Institute of Nephrology and UrologyHyderabad 500082India S.H.Ho UrologyCentre Department of SurgeryFaculty of MedicineThe Chinese University of Hong KongHong Kong 96H2+Q9China Department of Urology University Hospitals Southampton NHS TrustSouthampton SO166YDUnited Kingdom Department of Urology Cantonal Hospital Thurgau AGFraunfeld 8500Switzerland

We aim to evaluate the incidence of incontinence following laser endoscopic enucleation of the prostate(EEP)comparing en-bloc(Group 1)versus 2-lobe/3-lobe techniques(Group 2).We performed a retrospective review of patients undergoing EEP for benign prostaticenlargement in 12 centers between January 2020 and January *** were presented as median and interquartile range(IQR).Univariable and multivariable logistic regression analysis was performed to evaluate factors associated with stress urinary incontinence(SUI)and mixed urinary incontinence(MUI).There were 1711 patients in Group 1 and 3357 patients in Group *** in Group 2were significantly younger(68[62–73]years vs 69[63–74]years,P=0.002).Median(interquartile range)prostate volume(PV)wassimilar between the groups(70[52–92]ml in Group 1 vs 70[54–90]ml in Group 2,P=0.774).There was no difference in preoperativeInternational Prostate Symptom Score,quality of life,or maximum flow ***,morcellation,and total surgical time weresignificantly shorter in Group *** 1 month,overall incontinence rate was 6.3%in Group 1 versus 5.3%in Group 2(P=0.12),and urge incontinence was significantly higher in Group 1(55.1%vs 37.3%in Group 2,P<0.001).After 3 months,the overall rate ofincontinence was 1.7%in Group 1 versus 2.3%in Group 2(P=0.06),and SUI was significantly higher in Group 2(55.6%vs 24.1%in Group 1,P=0.002).At multivariable analysis,PV and IPSS were factors significantly associated with higher odds of transient SUI/***,surgical time,and no early apical release technique were factors associated with higher odds of persistent SUI/MUI.

关键词： benign prostatic hyperplasia en-bloc enucleation endoscopic enucleation of the prostate laser therapy stress urinaryincontinence urge urinary incontinence

Concerns about the application of resistance exercise with blood-flow restriction and thrombosis risk in hemodialysis patients

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Journal of Sport and Health Science 2024年第4期13卷 548-558,F0003页

作者： Hugo de Luca Correa Lysleine Alves Deus Dahan da Cunha Nascimento Nicholas Rolnick Rodrigo Vanerson Passos Neves Andrea Lucena Reis Thais Branquinho de Araujo Carmen Tzanno-Martins Fernanda Silveira Tavares Luiz Sinesio Silva Neto Claudio Avelino Rodrigues Santos Paolo Lucas Rodrigues-Silva fernando Honorato Souza Vitoria Marra da Motta Vilalva Mestrinho Rafael Lavarini dos Santos Rosangela Vieira Andrade Jonato Prestes Thiago dos Santos Rosa Post-graduate Program of Physical Education Catholic University of BrasıliaBrasılia 71966-700Brazil The Human Performance Mechanic Lehman CollegeNew YorkNY 10011USA Clinical Group Home Dialysis Center and RenalClass Sao Paulo 01239-040Brazil Department of Medicine Catholic University of BrasıliaBrasılia 71966-700Brazil Faculty of Medicine Federal University of TocantinsTocantins 77001-090Brazil Embrapa Genetic Resources and Biotechnology Brasılia 70770-917Brazil Graduate Program of Genomic Sciences and Biotechnology Brasılia 71966-700Brazil

Background:Hemodialysis(HD) per se is a risk factor for *** the growing body of evidence on blood-flow restriction(BFR) exercise in HD patients,identification of possible risk factors related to the prothrombotic agent D-dimer is required for the safety and feasibility of this training *** aim of the present study was to identify risk factors associated with higher D-dimer levels and to determine the acute effect of resistance exercise(RE) with BFR on this ***:Two hundred and six HD patients volunteered for this study(all with a glomerular filtration rate of 70 years old),and HD ***,RE+BFR significantly increases D-dimer after 4 *** with borderline baseline D-dimer levels(400-490 ng/mL) displayed increased risk of elevating D-dimer over the normal range(≥500 ng/mL).Conclusion:These results identified factors associated with a heightened prothrombotic state and may assist in the screening process for HD patients who wish to undergo RE+BFR.D-dimer and/or other fibrinolysis factors should be assessed at baseline and throughout the protocol as a precautionary measure to maximize safety during RE+BFR.

关键词： Blood-flow restriction Chronic kidney disease Coagulation Hemodialysis Vascular occlusion exercises

Oxidized organic molecules in the tropical free troposphere over Amazonia

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National Science Review 2024年第1期11卷 174-184页

作者： Qiaozhi Zha Diego Aliaga Radovan Krejci Victoria A.Sinclair Cheng Wu Giancarlo Ciarelli Wiebke Scholz Liine Heikkinen Eva Partoll Yvette Gramlich Wei Huang Markus Leiminger Joonas Enroth Otso Per?kyl? Runlong Cai Xuemeng Chen Alkuin Maximilian Koenig fernando Velarde Isabel Moreno Tuukka Pet?j? Paulo Artaxo Paolo Laj Armin Hansel Samara Carbone Markku Kulmala Marcos Andrade Douglas Worsnop Claudia Mohr Federico Bianchi Institute for Atmospheric and Earth System Research/Physics University of Helsinki Joint International Research Laboratory of Atmospheric and Earth System Sciences Nanjing University Department of Environmental Science & Bolin Centre for Climate Research Stockholm University Department of Chemistry and Molecular Biology University of Gothenburg Institute for Ion and Applied Physics University of Innsbruck Ionicon Analytik GmbH Laboratory for Atmospheric Physics Institute for Physics Research Universidad Mayor de San Andrés Institute of Physics University of Sao Paulo Institute for Geosciences and Environmental Research (IGE) University of Grenoble Alpes Agrarian Sciences Institute Federal University of Uberl?ndia Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Department of Atmospheric and Oceanic Sciences University of Maryland Aerodyne Research Inc. Department of Environmental System Science ETH Zürich Switzerland and Laboratory of Atmospheric Chemistry Paul Scherrer Institute

New particle formation(NPF) in the tropical free troposphere(FT) is a globally important source of cloud condensation nuclei, affecting cloud properties and climate. Oxidized organic molecules(OOMs)produced from biogenic volatile organic compounds are believed to contribute to aerosol formation in the tropical FT, but without direct chemical observations. We performed in situ molecular-level OOMs measurements at the Bolivian station Chacaltaya at 5240 m above sea level, on the western edge of Amazonia. For the first time, we demonstrate the presence of OOMs, mainly with 4–5 carbon atoms, in both gas-phase and particle-phase(in terms of mass contribution) measurements in tropical FT air from Amazonia. These observations, combined with air mass history analyses, indicate that the observed OOMs are linked to isoprene emitted from the rainforests hundreds of kilometers away. Based on particle-phase measurements, we find that these compounds can contribute to NPF, at least the growth of newly formed nanoparticles, in the tropical FT on a continental scale. Thus, our study is a fundamental and significant step in understanding the aerosol formation process in the tropical FT.

关键词： oxidized organic molecules tropical aerosol formation Amazon free troposphere

Elucidating regulatory processes of intense physical activity by multi-omics analysis

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Military Medical Research 2024年第4期11卷 479-499页

作者： Ernesto S.Nakayasu Marina A.Gritsenko Young-Mo Kim Jennifer E.Kyle Kelly G.Stratton Carrie D.Nicora Nathalie Munoz Kathleen M.Navarro Daniel Claborne Yuqian Gao Karl K.Weitz Vanessa L.Paurus Kent J.Bloodsworth Kelsey A.Allen Lisa M.Bramer fernando Montes Kathleen A.Clark Grant Tietje Justin Teeguarden Kristin E.Burnum-Johnson Biological Sciences Division Pacific Northwest National LaboratoryRichlandWA 99352USA Environmental and Molecular Sciences Division Pacific Northwest National LaboratoryRichlandWA 99352USA Centers for Disease Control and Prevention National Institute for Occupational Safety and HealthWestern States DivisionDenverCO 80204USA Computational Analytics Division Pacific Northwest National LaboratoryRichlandWA 99352USA National Security Directorate Pacific Northwest National LaboratoryRichlandWA 99352USA Los Angeles County Fire Department Los AngelesCA 90063USA Centers for Disease Control and Prevention National Institute for Occupational Safety and HealthRespiratory Health DivisionMorgantownWV 26505USA Environmental and Molecular Toxicology Oregon State UniversityCorvallisOR 97331USA

Background:Physiological and biochemical processes across tissues of the body are regulated in response to the high demands of intense physical activity in several occupations,such as firefighting,law enforcement,military,and sports.A better understanding of such processes can ultimately help improve human performance and prevent illnesses in the work ***:To study regulatory processes in intense physical activity simulating real-life conditions,we performed a multi-omics analysis of 3 biofluids(blood plasma,urine,and saliva)collected from 11 wildland firefighters before and after a 45 min,intense exercise *** profiles ***-exercise were compared by Student’s t-test followed by pathway analysis and comparison between the different omics ***:Our multi-omics analysis identified and quantified 3835 proteins,730 lipids and 182 metabolites combining the 3 different types of *** blood plasma analysis revealed signatures of tissue damage and acute repair response accompanied by enhanced carbon metabolism to meet energy *** urine analysis showed a strong,concomitant regulation of 6 out of 8 identified proteins from the renin-angiotensin system supporting increased excretion of catabolites,reabsorption of nutrients and maintenance of fluid *** saliva,we observed a decrease in 3 pro-inflammatory cytokines and an increase in 8 antimicrobial peptides.A systematic literature review identified 6 papers that support an altered susceptibility to respiratory ***:This study shows simultaneous regulatory signatures in biofluids indicative of homeostatic maintenance during intense physical activity with possible effects on increased infection susceptibility,suggesting that caution against respiratory diseases could benefit workers on highly physical demanding jobs.

关键词： Multi-omics analysis Intense exercise Human performance Biofluids Metabolism Immunity

Surface Soil Properties Influence Carbon Oxide Pulses After Precipitation Events in a Semiarid Vineyard Under Conventional Tillage and Cover Crops

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Pedosphere 2016年第4期26卷 499-509页

作者： fernando PEREGRINA CSIC-CAR-UR Instituto de Ciencias de la Vid y el Vino(ICVV)

In semiarid regions of the Mediterranean basin, a rainfall event can induce a respiratory pulse that releases a large amount of soil carbon dioxide （CO2） into the atmosphere; this pulse can significantly contribute to the annual ecosystem carbon （C） balance. The impacts of conventional tillage and two different cover crops, resident vegetation and Bromus catharticus L., on soil CO2 effiux were evaluated in a Vitis vinifera L. vineyard in La Rioja, Spain. Soil CO2 efflux, gravimetric water content, and temperature were monitored at a depth of 0-5 cm after rainfall precipitation events approximately every 10 d in the period from May 17 to July 27, 2012, during which the cover crops had withered. Additionally, on June 10, 2012, soil organic C, microbial biomass C, and I^-glucosidase activity were determined at soil depths of 0-2.5, 2.5-5, 5-15, and 15-25 cm. The results show that pulses of soil CO2 were related to the increase in soil water content following precipitation events. Compared to the conventional tillage treatment, both cover crop treatments had higher soil CO2 efflux after precipitation events. Both cover crop treatments had higher soil organic C, microbial biomass C, and β-glucosidase activity at the soil surface （0-2.5 cm） than the conventional tillage treatment. Each pulse of CO2 was related to the surface soil properties. Thus, this study suggests that the enhancement of soil organic C and microbiological properties at the soil surface under cover crops may increase soil CO2 efflux relative to conventional tillage immediately after precipitation events during the dry season.

关键词： microbial biomass C β-glucosidase activity soil CO2 effiux soil water content soil temperature

Dinophysoid dinoflagellates from subphotic depths:Amphisolenia ***,Dinofurcula tricornuta ***.,and Dinophysis profunda ***.(Dinophysales,Dinophyceae)

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Journal of Oceanology and Limnology 2022年第2期40卷 703-711页

作者： fernando GÓMEZ Department of Biological and Environmental Sciences and Technologies University of SalentoLecce 73100Italy

Three species of dinophysoid planktonic dinoflagellates are described from the eastern Mediterranean *** *** was collected at 70-m depth in the Ionian *** small species showed a more asymmetric midbody and a proportionally longer neck when compared to the closer relative *** individuals of Dinofurcula tricornuta *** at 154-m depth in the Marmara Sea are characterized by three posterior-oriented processes when compared to its congeneric *** is the first record of the genus Dinofurcula beyond the eastern tropical Pacific *** profunda *** at 500-m depth in the Ionian Sea is characterized by ovate hypotheca and a serrate crest-like left sulcal list when compared to its close relative *** findings evidence an undescribed biodiversity in the under-sampled subphotic depths.

关键词： deep ocean Dinophyta Dinoflagellata Mediterranean Sea mesopelagic new species

Photoinactivation of Escherichia coli using five photosensitizers and the same number of photons

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Journal of Innovative Optical Health Sciences 2022年第6期15卷 53-67页

作者： Daniel Ortega-Zambrano Denisse Fuentes-López Hilda Mercado-uribe CINVESTAV-Monterrey Parque PIITAutopista al Aeropuerto km.9.5ApodacaNL 66600Mexico

Bacterial resistance is today a matter of great medical concern,so it is urgent to investigate alternatives to alleviate *** inactivation(PDI)is a method that has been endorsed to inactivate diferent pathogens,including bacteria,fungi and *** is achieved by using a photosensitizer(PS)molecule which generates reactive oxygen species under visible or UV *** use visible light and UV-A radiation to excite four commercial PSs(methylene blue,rose bengal,riboflavin and curcumin),and nanopartides synthesized in our *** these PSs having been thoroughly studied in the past by other research groups,in order to compare their effects in an appropriate way,we matched the number of photons they *** found that methylene blue leads to the major inactivation of Escherichia ***,we evaluated the production of singlet axygen and hydroxyl radicals in the photoinactivation process.

关键词： Photodynamic inactivation E.coli ROS photons

Integrating VGI and 2D hydraulic models into a data assimilation framework for real time flood forecasting and mapping

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Geo-Spatial Information Science 2019年第4期22卷 223-236,I0001页

作者： Antonio Annis fernando Nardi Water Resources Research and Documentation Centre(WARREDOC) University for Foreigners of PerugiaPerugiaItaly Department of Civil and Environmental Engineering(DICEA) University of FlorenceFlorenceItaly Institute of Water and Environment(InWE) Florida International UniversityMiamiFLUSA

Crowdsourced data can effectively observe environmental and urban ecosystem *** use of data produced by untrained people into flood forecasting models may effectively allow Early Warning Systems(EWS)to better perform while support decision-making to reduce the fatalities and economic losses due to inundation *** this work,we develop a Data Assimilation(DA)method integrating Volunteered Geographic Information(VGI)and a 2D hydraulic model and we test its *** proposed framework seeks to extend the capabilities and performances of standard DA works,based on the use of traditional in situ sensors,by assimilating VGI while managing and taking into account the uncertainties related to the quality,and the location and timing of the entire set of observational *** November 2012 flood in the Italian Tiber River basin was selected as the case *** show improvements of the model in terms of uncertainty with a significant persistence of the model updating after the integration of the VGI,even in the case of use of few-selected observations gathered from social *** will encourage further research in the use of VGI for EWS considering the exponential increase of quality and quantity of smartphone and social media user worldwide.

关键词： Crowdsourced data VGI data assimilation(DA) flood forecasting 2D hydraulic modelling

Cell-free systems in the new age of synthetic biology

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Frontiers of Chemical Science and Engineering 2017年第1期11卷 58-65页

作者： fernando Villarreal Cheemeng Tan Department of Biomedical Engineering University of California Davis Davis One Shields Avenue CA 95616 USA

The advent of synthetic biology has ushered in new applications of cell-free transcription-translation systems. These cell-free systems are reconstituted using cellular proteins, and are amenable to modular control of their composition. Here, we discuss the historical advance- ment of cell-free systems, as well as their new applications in the rapid design of synthetic genetic circuits and components, directed evolution ofbiomolecules, diagnosis of infectious diseases, and synthesis of vaccines. Finally, we present our vision on the future direction of cell-free synthetic biology.

关键词： cell-free system application