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中国蚕学会第九届青年学术研讨会

作者： Min-Jin Han Qiu-Zhong Zhou Hua-Hao Zhang Xiaoling Tong Cheng Lu Ze Zhang fangyin dai State Key Laboratory of Silkworm Genome Biology Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural MinistrySouthwest University Laboratory of Evolutionary and Functional Genomics School of Life SciencesChongqing University College of Pharmacy and Life Science Jiujiang University

Miniature inverted-repeat transposable elements（MITEs） have attracted much attention due to their widespread occurrence and high copy numbers in eukaryotic ***,the systematic knowledge about MITEs in insects and other animals is still *** this study,we identified 6012 MITE families from 98 insect species *** of these MITEs with known MITEs in the NCBI non-redundant database and Repbase showed that 5701（95%） of 6012 MITE families are *** abundance of MITEs varies drastically among different insect species,and significantly correlates with genome *** general,larger genomes contain more MITEs than small ***,all identified MITEs were included in a newly constructed database（iMITEdb）（http://***/iMITEdb/）,which has functions such as browse,search,BLAST and ***,our results not only provide insight on insect MITEs but will also improve assembly and annotation of insect *** importantly,the results presented in this study will promote studies of MITEs function,evolution and application in insects.

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Cuticular protein defective Bamboo mutant of Bombyx mori is sensitive to environmental stresses

Cuticular protein defective Bamboo mutant of Bombyx mori is ...

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中国蚕学会第十届青年学术研讨会

作者： Gao Xiong Xiaoling Tong Zhengwen Yan Hai Hu Xiaohui Duan Chunlin Li Minjin Han Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory of Sericultural Biology and Genetic Breeding Ministry of AgricultureCollege of BiotechnologySouthwest University

Insect cuticle acts as a primary protective barrier against environment stresses that may directly impact the insect ***,we report the mechanical defense function of a structural cuticular protein,BmorCPH24,to environmental stresses using a silkworm Bamboo（Bo） mutant with this gene ***（UV） irradiation and topical application of an acetone insecticide were used as environmental stresses to determine the differences in susceptibility between Bo and wild-type *** irradiation resulted in a sunburn phenotype in the Bo strains earlier than the wild-type indicating the sensitivity of *** malondialdehyde（MDA） content and a lower survival ratio were also observed in the Bo *** with deltamethrin revealed that Bo larvae were more sensitive to insecticides than the ***,cuticle analysis by microsection revealed thinner cuticle and a significant decrease in the endocuticle layer（～64.0%） in *** results suggest that BmorCPH24 mutation can lead to deficiency in resources required to construct the cuticle in Bo resulting in thin cuticle and reduced resistance to UV and *** results provide us new insight into the role of structural cuticular proteins in insect cuticle against environment stresses.

关键词： Bombyx mori Cuticle thickness Cuticular protein Environmental stress Resistance

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Hippo pathway regulates somatic development and cell proliferation of silkworm

Hippo pathway regulates somatic development and cell prolife...

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2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Niannian Li Xiaoling Tong Jie Zeng Gang Meng Fuze Sun Hai Hu Jiangbo Song Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural MinistrySouthwest University

Hippo signaling pathway（signaling pathway Hippo, hereinafter referred to as the Hippo pathway） was the earliest found in Drosophila, which can regulate the development of tissues and organs, even some components of the pathway were identified as tumor suppressor. The pathway was more concerned in fruit flies and mice, but little attention has been given in silkworm, an important economic and lepidopteran model insect. In this manuscript, we identified major Hippo pathway related genes（Hippo, Salvador, Warts, Mats, Yorkie） in silkworm and named Bm Hpo, BmSav, BmWts, BmMats, BmYki. The domain organization of these genes was highly conserved in silkworm and other organisms suggesting that they could use similar proteinprotein interactions to construct the Hippo kinase cascades. The expression profiles of these genes in silkworm during embryonic, larval, wandering, pupal and adult stages were analyzed, 14 tissues/organs of the day 3, 5 th instar larvae（L5 D3） as well. Experimental results showed that the expression of Hippo pathway had some influence on the development of silkworm. In order to find out the mechanism of Hippo pathway affecting silkworm development, BmHpo and Bm Yki were up-regulated and de-regulated in the cell line of Bombyx mori-BmN-SWU1（NS）, and the changes of cell proliferation activity and cell cycle were detected. The distribution of BmYki was detected by immunofluorescence technique. This study provides insights into the genes of Hippo pathway which have a certain effect on somatic development and cell proliferation in silkworm.

关键词： Hippo pathway development cell proliferation cell cycle silkworm

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Variation of lifespan in multiple strains,and effects of dietary restriction and BmFoxO on lifespan in silkworm,Bombyx mori

Variation of lifespan in multiple strains,and effects of die...

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中国蚕学会第十届青年学术研讨会

作者： Jiangbo Song Dongmei Tang Zhiquan Li Xiaoling Tong Jianfei Zhang Minjin Han Hai Hu Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology College of BiotechnologySouthwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry College of BiotechnologySouthwest University

Established animal models have accelerated our understanding of the mechanisms involved in lifespan ***,more experimental animals are required to clarify the complex mechanisms behind the phenomena of aging and *** this study,we reported the variation of lifespan in nine distinct silkworm *** correlated significantly with BmFoxO gene expression in the representative silkworm strains tested（Xiafang,Dazao-N,and N4）.In general,the female silkworm was longer lived than the male of the same *** restriction extended the silkworm lifespan compared with that of silkworms fed ad *** expression of BmFoxO was significantly elevated in the dietary restriction group on day 3 of the 4th instar and day 3 of the 5th instar,suggesting that BmFoxO contributes to dietary restriction-mediated lifespan *** RNA interference and overexpression of the BmFoxO gene significantly shortened and extended the silkworm adulthood,*** conclusion,our findings suggest that the silkworm might serve as a promising experimental animal to explore the complex biological mechanisms of lifespan determination.

关键词： BmFoxO Gerotarget dietary restriction experimental animal lifespan silkworm

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Rhodiola rosea extends lifespan and improves stress tolerance in silkworm,Bombyx mori

Rhodiola rosea extends lifespan and improves stress toleranc...

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中国蚕学会第九届青年学术研讨会

作者： Cong Chen Jiangbo Song Min Chen Zhiquan Li Xiaoling Tong Hai Hu Zhonghuai Xiang Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry Southwest University

The root of Rhodiola rosea is widely used in Traditional Chinese *** extract from *** is reported to extend the lifespan of yeast,nematode,and fruit ***,the molecular mechanism is not fully ***,we tested whether *** extends the lifespan of the *** aqueous extract of *** significantly prolonged the lifespan of the silkworm,without affecting its daily food intake,body weight,or fecundity,suggesting that *** did not exhibit obvious side *** rosea extract also enhanced the stress resistance in the silkworm,against heat stress（37 ℃） and *** *** extract increased the activity of the major antioxidant enzymes,glutathione S-transferase and catalase,and altered the content of glutathione and *** rosea increased the expression of Bm FoxO,which is a downstream regulator of insulin/IGF-1 signaling（IIS） pathway in the *** results showed that *** extends lifespan,in which IIS pathway might be involved,and enhances stress resistance in the ***,the silkworm might be used as a novel animal model for lifespan study and efficacy evaluation of Traditional Chinese Medicines.

关键词： Rhodiola rosea Lifespan Oxidative stress IIS pathway Bombyx mori

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Hippo pathway regulates somatic development and cell proliferation of silkworm

Hippo pathway regulates somatic development and cell prolife...

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中国蚕学会第十届青年学术研讨会

Hippo signaling pathway（signaling pathway Hippo,hereinafter referred to as the Hippo pathway） was the earliest found in Drosophila（Schneck [1]）,which can regulate the development of tissues and organs,even some components of the pathway were identified as tumor suppressor [2].The pathway was more concerned in fruit flies and mice（Schneck [1]）,but little attention has been given in silkworm,an important economic and lepidopteran model *** this manuscript,we identified major Hippo pathway related genes（Hippo,Salvador,Warts,Mats,Yorkie） in silkworm and named BmHpo,BmSav,BmWts,BmMats,*** domain organization of these genes was highly conserved in silkworm and other organisms suggesting that they could use similar protein-protein interactions to construct the Hippo kinase *** expression profiles of these genes in silkworm during embryonic,larval,wandering,pupal and adult stages were analyzed,14 tissues/organs of the day 3,5th instar larvae（L5D3） as *** results showed that the expression of Hippo pathway had some influence on the development of *** order to find out the mechanism of Hippo pathway affecting silkworm development,BmHpo and BmYki were up-regulated and de-regulated in the cell line of Bombyx mori-BmN-SWU1（NS）,and the changes of cell proliferation activity and cell cycle were *** distribution of BmYki was detected by immunofluorescence *** study provides insights into the genes of Hippo pathway which have a certain effect on somatic development and cell proliferation in silkworm.

关键词： Hippo pathway Development Cell proliferation Cell cycle Silkworm

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蚕丝及蚕丝织物的改性研究综述

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蚕业科学 2016年第6期42卷 1106-1112页

作者：马艳李智代方银程岚西南大学纺织服装学院重庆400715

蚕丝及蚕丝织物的改性是为了克服天然蚕丝蛋白纤维自身的性能缺陷或赋予其新的功能,达到改善蚕丝及蚕丝织物的服用性能并适合不同用途的目的。蚕丝及其织物改性的方法有物理方法、化学方法、养蚕添食法和分子生物学方法。其中,物理方法... 详细信息

蚕丝及蚕丝织物的改性是为了克服天然蚕丝蛋白纤维自身的性能缺陷或赋予其新的功能,达到改善蚕丝及蚕丝织物的服用性能并适合不同用途的目的。蚕丝及其织物改性的方法有物理方法、化学方法、养蚕添食法和分子生物学方法。其中,物理方法主要采用等离子体技术、高温或低温处理以及辐照处理等进行改性;化学方法是利用蚕丝蛋白分子链上的活性基团,通过化学反应引入目的功能基团、单体或大分子链,从而改变蚕丝性能;养蚕添食法是在家蚕幼虫饲养过程中添食色素、荧光材料或金属纳米颗粒,获得改性蚕丝;分子生物学方法则是基于转基因技术或基因编辑技术让家蚕吐出具有特殊性能的茧丝。本文综述近年来利用上述几种方法,尤其是利用分子生物学技术进行蚕丝及蚕丝织物改性研究取得的重要进展,在此基础上分析了这几种方法各自的特点、适用范围,并展望了未来蚕丝及蚕丝织物改性研究的发展方向。

关键词：蚕丝改性分子生物学方法物理方法化学方法养蚕添食法

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Morphological characterization and molecular mapping of an irradiation-induced Speckled mutant in Silkworm,Bombyx mori

Morphological characterization and molecular mapping of an i...

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中国蚕学会第九届青年学术研讨会

作者： Duan Tan Xiaoling Tong Hai Hu Songyuan Wu Chunlin Li Gao Xiong Zhonghuai Xiang fangyin dai Cheng Lu State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry Southwest University

Speckled（Spc）,an X-ray-induced lethal mutant of Bombyx mori,exhibits a mosaic dark-brown-spotted larval epidermis in both sexes and egg-laying problems only in ***,we report the morphological characterization and molecular mapping of the Spc *** investigations revealed that the epidermal ultrastructure of the small,dark-brown spots was more dense than that of the white regions in both Spc/+ mutants and wild type,and that the lethality of the Spc/Spc mutants occurred during early ***,the ovarioles and ovipositor were disconnected in approximately 85.5% of Spc/+ females,a further 2.5% had a connection between the ovarioles and ovipositor that was too narrow to lay *** remaining females showed a normal connection similar to that of the wild *** successfully narrowed down the location of the Spc mutation to a region on chromosome 4 that was ～1041 kb ***-prediction analysis identified 25 candidate genes in this *** structure analysis indicated that a ～305-kb deletion was included in the mapping *** and spatial reverse transcription（RT-PCR） analysis showed that several genes in the mapped region are associated with the Spc *** the genes responsible for the Spc mutation were not definitively identified,our results further the current understanding of the complex mechanism underlying the multiple morphological defects in Spc mutants.

关键词： Bombyx mori Speckled mutant phenotypic analysis molecular mapping autosomal fragment deletion

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Disruption of PTPS Gene Causing Pale Body Color and Lethal Phenotype in the Silkworm,Bombyx mori

Disruption of PTPS Gene Causing Pale Body Color and Lethal P...

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中国蚕学会第十届青年学术研讨会

作者： Xiaoling Tong Pingfeng Liang Songyuan Wu Yuanhao Li Liang Qiao Hai Hu Zhonghuai Xiang Cheng Lu fangyin dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericulture Biology and Genetic BreedingAgricultural MinistryCollege of BiotechnologySouthwest University College of Biotechnology Southwest University

Phenylketonuria（PKU） is an inborn error of metabolism caused by mutations in the phenylalanine hydroxylase（PAH） gene or by defects in the tetrahydrobiopterin（BH4） synthesis ***,by positional cloning,we report that the 6-pyruvoyl-tetrahydropterin synthase（PTPS） gene,encoding a key enzyme of BH4 biosynthesis,is responsible for the alc（albino C） mutation that displays pale body color,head shaking,and eventually lethality after the first molting in *** to wild type,the alc mutant produced more substrates（phenylalanine（Phe） and tyrosine（Tyr）） and generated less DOPA and *** of 2,4-diamino-6-hydroxypyrimidine（DAHP） to block BH4 synthesis in the wild type effectively produced the alc-like phenotype,while BH4 supplementation rescued the defective body color and lethal phenotype in both alc and DAHP-treated *** detection of gene expressions and metabolic substances after drugs treatments in alc and normal individuals imply that silkworms and humans have a high similarity in the drugs metabolic features and the gene pathway related to BH4 and the dopamine *** propose that the alc mutant could be used as an animal model for drug evaluation for BH4-deficient PKU.

关键词： silkworm model alc PTPS BH4 phenylketonuria

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添食法制备改性蚕丝的研究进展

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材料导报 2020年第23期34卷 23190-23198页

作者：蔡海华程岚李智陈李童晓玲代方银西南大学家蚕基因组生物学国家重点实验室西南大学生物技术学院农业部蚕桑生物学与遗传育种重点实验室西南大学纺织服装学院重庆市生物质纤维材料与现代纺织工程技术研究中心

蚕丝是优异的天然蛋白质纤维,被广泛地应用在纺织、服装、生物医学等领域。蚕丝的形成需经历一个复杂的过程:家蚕首先在后部丝腺合成丝素蛋白,丝素蛋白液经过中部丝腺,与其合成分泌的丝胶液一起,通过腺腔内的压力进入到前部丝腺、吐丝管... 详细信息

蚕丝是优异的天然蛋白质纤维,被广泛地应用在纺织、服装、生物医学等领域。蚕丝的形成需经历一个复杂的过程:家蚕首先在后部丝腺合成丝素蛋白,丝素蛋白液经过中部丝腺,与其合成分泌的丝胶液一起,通过腺腔内的压力进入到前部丝腺、吐丝管,再通过榨丝区的肌肉活动对丝蛋白受到的剪切力进行调节,使其二级结构改变,最后在家蚕头部的牵伸摆动下,迅速固化,形成蚕丝。蚕丝虽然具有优异的性能,但仍存在抗菌性能及抗紫外性能差等缺陷,这在一定程度上限制了蚕丝的应用及蚕丝产业的进一步发展。通过对蚕丝进行改性并赋予其新的功能可拓展蚕丝的应用领域,促进蚕丝产业的发展。添食法具有简单、高效、环保及有利于规模化生产等优点,可在源头改性蚕丝的性能,是大量制备改性蚕丝的最有效方法。添食物质的选择至关重要。首先,添食物质不能有毒性;添食后,不能影响家蚕正常的生理及生命活动。其次,要注意添食物质颗粒的尺寸。此外,选择的添食物质需要有突出的性能。综合以上因素,纳米颗粒成为添食家蚕的首选物质。研究者们选择不同种类的纳米颗粒进行添食,取得了一定的成果。在保证蚕丝质地柔软光滑本质的同时,能大幅提升蚕丝的力学性能,且改善蚕丝的热稳定性能。目前,通过添食纳米颗粒制备的蚕丝的力学性能最高可提高111.25%。荧光蚕丝在生物医药工程、光学和光电子领域都具有巨大的应用价值。近年来,有多种方法已应用于制备荧光蚕丝,但大都不够环保且难以大规模持续生产。研究人员通过给五龄时期的家蚕添食具有荧光效应的染料或稀土荧光粉的方法可获得彩色荧光蚕丝。通过此方法所得的荧光蚕丝不仅具有良好的荧光性能,而且具有优异的荧光稳定性。但添食荧光材料会对家蚕的生理和生命活动造成一定影响,并使蚕丝的力学性能有所降低。为了制备具有不同功能的蚕丝,研究人员拓展了添食物质的种类,利用含氮物质、超细羽绒粉体、色素等进行添食,得到了质量突出的蚕茧及力学性能优异的改性蚕丝。本文以添食物质、添食后制备的改性蚕丝的结构及性能为重点,综述了近年来利用纳米物质、荧光物质及其他物质添食制备改性蚕丝的研究进展,客观分析了不同添食物质对蚕丝结构、力学性能及热性能等的影响,展望了添食法未来的研究及发展方向。

关键词：蚕丝改性添食法纳米颗粒荧光材料

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