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Journal of Integrative Agriculture 2014年第10期13卷 2211-2220页

作者： ZHANG Xiao-ming YANG Nian-wan wan fang-hao Gabor L Lvei State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Department of Agroecology Aarhus UniversityFlakkebjerg Research Centre DK-4200 SlagelseDenmark

The density seasonal dynamics of Bemisia tabaci MED were evaluated over two years in a cotton-growing area in Langfang, Hebei Province, northern China on cotton （Gossypium hirsutum L.） and six other co-occurring common plants, common ragweed （Ambrosia artemisiifolia L.）, piemarker （Abutilon theophrasti Medicus）, sunflower （Helianthus annuus L.）, sweet potato （Ipomoea batatas L.）, soybean （Glycine max L.）, and maize （Zea mays L.）. The whitefly species identity was repeatedly tested and confirmed; seasonal dynamics on the various host plants were standardized by the quartile method. B. tabaci MED appeared on weeds （the common ragweed and piemarker） about 10 days earlier than on cotton, or the other cultivated plants. The peak population densities were observed over a span of 2 to 3 weeks on cotton, starting in early （2010） or mid-August （2011）. The common ragweed growing adjacent to cotton supported the highest B. tabaci densities （no. on 100 cm2 leaf surface）, 12-22 fold higher than on cotton itself. Sunflower supported more B. tabaci than the other plants, and about 1.5-2 fold higher than cotton did, Our results indicate that weeds （esp. the common ragweed） around cotton fields could increase the population density of B. tabaci MED on cotton, while sunflower could act as a trap crop for decreasing pest pressure on cotton.

关键词： Bemisia tabaci whitefly cotton sunflower ragweed population dynamics seasonal dynamics quartile method

SPECIES-SPECIFIC MTCOI MAKER FOR RAPID DIAGNOSE OF INVASIVE SPEICES,FRANKLINIELLA OCCIDENTALIS (PERGANDE)

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SPECIES-SPECIFIC MTCOI MAKER FOR RAPID DIAGNOSE OF INVASIVE ...

DISTRIBUTION CHARACTERISTICS OF AGERATINA ADENOPHORA (COMPOSITAE) ROOTS IN DIFFERENT INVADED AREAS

International Congress on Biological Invasions

作者： Gui-Fen ZHANG~(1,2),Xun LIU~1,Xiang-Qin MENG~1,Liang MIN~1,and fang-hao wan~(1,2) 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China, 2 Center for Management of Invasive Alien Species,MOA,Beijing 100081,China

The western flower thrips,Frankliniella occidentalis(Pergande)(Thysanoptera: Thripidae),is an invasive species and currently occurs only in a few areas in China.A quick and correct and developmental-stage non-limiting method of the identification of the western flower thrips is essential for preventing the spread of this thrips. Morphological identification of thrips is performed on adults and to a lesser extent on second-stage larvae *** identification,however,can be easily followed by a non-thrips-specialist with a little training.A species-specific marker within the mitochondrial cytochrome oxidase I(COI) sequence for *** was developed in the present *** specific primers amplified a single band of 340 *** specificity of the primers was assessed using 28 other thrips species commonly occurring in *** *** specimens were detected,and no cross reactions with other thrips species were *** method was tested on single egg,first- and second-stage larvae,pre-pupae,pupae and adults,and proved to be applicable for all these ***,the method was used to clarify the distribution of the western flower thrips in Beijing metro area *** results suggested that the species-specific mtCOI marker without gene sequencing analysis is useful in the early detection and rapid identification of ***,and makes it possible for diagnostic testing and routine screening of samples at ports of entry and preventing new incursions and further damage of this thrips in China.

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DISTRIBUTION CHARACTERISTICS OF AGERATINA ADENOPHORA (COMPOS...

海氏桨角蚜小蜂对不同龄期“Q”烟粉虱的取食和寄生反应

International Congress on Biological Invasions

作者： Fu-Rong GUI~(1,2),wan-Xue LIU~2,and fang-hao wan~2 1 Key Laboratory for Agricultural Biodiversity and Pest Management of Ministry of Education, Plant Protection College,Yunnan Agricultural University,Kunming 650201,China 2 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,100081,China

The Crofton weed Ageratina adenophora(Spreng.) King & Robinson is a global worst invasive weed,it rapidly became the dominant flora in the invaded areas,where it threatened the native biodiversity and ecosystem,and caused serious economic *** present paper investigated and compared the distribution of A. adenophora roots in newly,moderate and heavily invaded areas,the objective is to find the distribution characteristics of roots in different invaded areas of ***. The results showed that,the mean root length density(RLD) of *** increasing following with its invading *** with moderate and heavily invaded areas of ***,roots system in newly invaded area is obviously under developed,their mainly exist during 0-10cm depth soil,and the mean RLD in this area visibly less than the above two invaded areas(p<0.01).RLD of A. adenophora in three invaded areas are all become few gradually following with the increasing distance from center of the samples,and root contents are distinctly differ in different horizontal distributions(P<0.01).On the vertical distribution,roots of A. adenophora in three invaded areas are all mainly exist during 0-10cm depth,and the root content of 10-20cm depth in the newly invaded areas is evidently lower than that of moderate and heavily invaded areas.

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生物安全学报 2012年第1期21卷 9-13页

作者：戴鹏阮长春臧连生万方浩吉林农业大学生物防治研究所天敌昆虫应用技术工程研究中心吉林长春130118 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193

【背景】近年来"Q"烟粉虱在我国快速扩张,已成为农业生产上的一种重要入侵害虫。由于化学农药的大量使用,"Q"烟粉虱已对多种农药产生高抗药性,保护和利用天敌昆虫对其进行控制具有重要意义。【方法】本文在室内研究了近年引入我国的海... 详细信息

【背景】近年来"Q"烟粉虱在我国快速扩张,已成为农业生产上的一种重要入侵害虫。由于化学农药的大量使用,"Q"烟粉虱已对多种农药产生高抗药性,保护和利用天敌昆虫对其进行控制具有重要意义。【方法】本文在室内研究了近年引入我国的海氏桨角蚜小蜂对不同龄期"Q"烟粉虱若虫的取食和寄生能力。【结果】各龄期的"Q"烟粉虱若虫均可被海氏桨角蚜小蜂取食和寄生。在48h内,寄生蜂取食1、2、3、4龄烟粉虱若虫的数量分别为10.7、6.4、6.7、5.0头,呈现随龄期增大取食数量下降的趋势;海氏桨角蚜小蜂对不同龄期烟粉虱若虫的寄生能力存在明显差异,其更偏好寄生2、3龄若虫(25.4和27.5头),其次是1龄若虫(22.1头),而寄生4龄粉虱的数量最低(16.5头)。【结论与意义】寄主龄期对海氏桨角蚜小蜂的取食和寄生能力具有显著影响。综合来看,该寄生蜂表现出对"Q"烟粉虱较好的生物防治潜能,是防治烟粉虱的理想寄生性天敌昆虫。

关键词：烟粉虱海氏桨角蚜小蜂取食寄主行为寄主选择生物防治

Rényi多样性指数曲线在评价和监测转基因作物对生物多样性影响中的应用(英文)

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生物安全学报 2013年第1期22卷 43-50页

作者： Gabor L.LOVEI 刘万学郭建英万方浩中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193 Department of Agroecology Aarhus UniversityFlakkebjerg Research Centre

【背景】多样性比较是评价转基因作物对生物群落影响的标准参数之一。以往用于描述多样性的指数很多,但这些指数各有优缺点,生物学解释不全面。运用现代生物多样性表示方法,能够描述更复杂情况下的多样性关系。本文主要介绍了Rényi多... 详细信息

【背景】多样性比较是评价转基因作物对生物群落影响的标准参数之一。以往用于描述多样性的指数很多,但这些指数各有优缺点,生物学解释不全面。运用现代生物多样性表示方法,能够描述更复杂情况下的多样性关系。本文主要介绍了Rényi多样性指数曲线在生物安全研究中的应用。【方法】Rényi多样性指数曲线与以往常用的一维多样性指数不同,当其等级参数为某些特定值时代表了几个著名的多样性指数,该方法可较为确切地评价复杂的生物群落的多样性。本文在介绍其计算公式后,通过比较河北省2种转Bt基因棉田(孟山都33B和中棉30,均未施农药)以及常规管理(施用农药)和综合治理(IPM,施用农药和释放赤眼蜂相结合)条件下非转基因棉田的蜘蛛群落,证明了该方法的适用性。【结果】Rényi多样性指数曲线分析表明了所有可能的互作类型:1个明确的多样性排序以及2类棉田蜘蛛群落间不同的潜在关系。Rényi指数分析表明,在供试棉田中,中棉30棉田的蜘蛛多样性指数最高,其他3种棉田蜘蛛的多样性高低则难以一概而论。蜘蛛稀有种的多样性以孟山都33B棉田最高,IPM和常规棉田次之;蜘蛛常见种的多样性以IPM棉田最高,常规棉田和孟山都33B棉田次之。【结论与意义】Rényi多样性指数可用于生物群落多样性的综合评价,并可用于评价不同管理措施对生物多样性的影响。该方法弥补了仅用单一数量参数评价多样性的传统方法的缺陷,将不同参数整合成一个具有内在关联的概念框架。

关键词： Bt棉可扩展多样性指数农药效应生物安全评价生物多样性比较 Rényi多样性指数

Species-specific COI primers for rapid identification of a globally significant invasive pest, the cassava mealybug Phenacoccus manihoti Matile-Ferrero

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Journal of Integrative Agriculture 2019年第5期18卷 1042-1049页

作者： wanG Yu-sheng TIAN Hu wan fang-hao ZHANG Gui-fen State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Integrated Pest Management of Crop Ministry of Agriculture/Institute of Plant Protection Chinese Academy of Agricultural Sciences Caofeidian Sub-Center of Hebei Entry-Exit Inspection and Quarantine Technical Center

The globally invasive cassava mealybug Phenacoccus manihoti Matile-Ferrero is a pernicious pest of cassava,and its recent introduction into Asia has raised considerable *** slow or prevent further invasion,an accurate,simple,and developmental-stage-independent detection method for *** is *** the present study,a PCR method based on a species-specific mitochondrial DNA cytochrome oxidase I(SS-COI)marker was developed for rapid identification of *** pair of SS-COI primers(PMSSZW-1F and PMSSZW-1R)was designed based on sequence variations in the COI gene among *** and related mealybug *** of the primer pair was validated on 21 closely related *** tests were performed on four immature developmental stages and female *** tests demonstrated that at the relatively low concentration of(135.2±14.7)pgresuspended DNA,the specific fragment was detected in all ***,the SS-COI primer pair was assayed on three populations of *** from major exporting countries of *** PCR assay was proved to be a rapid,simple,and reliable molecular measure for the identification of *** tool will be useful for quarantine,monitoring,and management of this invasive pest.

关键词： Phenacoccus manihoti cassava mealybug invasive pest molecular identification species-specific COI primers

国家自然科学基金资助的生物入侵基础研究概况

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生物安全学报 2011年第1期20卷 20-28页

作者：侯有明胡琼波万方浩尤民生福建农林大学植物保护学院农业部亚热带农业生物灾害与治理重点开放实验室国家自然科学基金委员会中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室

基于1999~2010年国家自然科学基金资助项目,分析了我国生物入侵基础研究概况。在这期间,生物入侵领域得到资助的项目数和经费均呈现快速上升的趋势,资助的项目从1999年的3个增加到2010年的69个,资助的经费从1999年的94万元增加到2010年... 详细信息

基于1999~2010年国家自然科学基金资助项目,分析了我国生物入侵基础研究概况。在这期间,生物入侵领域得到资助的项目数和经费均呈现快速上升的趋势,资助的项目从1999年的3个增加到2010年的69个,资助的经费从1999年的94万元增加到2010年的1838万元。得到资助的项目数在不同学科的分布不均匀,其中,植物保护学科得到资助的项目数为123个,生态学科得到资助的项目数为82个,这2个学科得到资助的项目数占总项目数的67.9%,得到资助的经费占总经费的66.3%。资助项目的主要研究内容涉及22种入侵植物病原微生物、26种入侵动物和24种入侵植物,从中可以透视本领域的研究重点。资助的项目主要集中在华北、华东和华南地区的国家级科研机构和高等院校,这种区域分布格局与我国入侵生物的地理分布格局相吻合。为了进一步推动国家自然科学基金对生物入侵领域各项工作的资助,本文提出了一些合理化的建议。

关键词：生物入侵基础研究国家自然科学基金资助情况

Estimation of the potential geographical distribution of a new potato pest(Schrankia costaestrigalis) in China under climate change

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Journal of Integrative Agriculture 2023年第8期22卷 2441-2455页

作者： XIAN Xiao-qing Zhao hao-xiang GUO Jian-yang ZHANG Gui-fen LIU Hui LIU wan-xue wan fang-hao State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193P.R.China The National Agro-Tech Extension and Service Center Beijing 100193P.R.China

Global food security is threatened by the impacts of the spread of crop pests and changes in the complex interactions between crops and pests under climate *** costaestrigalis is a newly-reported potato pest in southern ***-warning monitoring of this insect pest could protect domestic agriculture as it has already caused regional yield reduction and/or quality decline in potato *** research aimed to confirm the potential geographical distributions(PGDs)of *** in China under different climate scenarios using an optimal MaxEnt model,and to provide baseline data for preventing agricultural damage by *** findings indicated that the accuracy of the optimal MaxEnt model was better than the default-setting model,and the minimum temperature of the coldest month,precipitation of the driest month,precipitation of the coldest quarter,and the human influence index were the variables significantly affecting the PGDs of *** highly-and moderately-suitable habitats of *** were mainly located in eastern and southern *** PGDs of *** in China will decrease under climate *** conversion of the highly-to moderately-suitable habitat will also be significant under climate *** centroid of the suitable habitat area of *** under the current climate showed a general tendency to move northeast and to the middle-high latitudes in the *** agricultural practice of plastic film mulching in potato fields will provide a favorable microclimate for *** in the suitable *** attention should be paid to the early warning and monitoring of *** in order to prevent its further spread in the main areas in China’s winter potato planting regions.

关键词： climate change potential geographical distribution crop insect pests Schrankia costaestrigalis MaxEnt model

Enrichment Mechanism and Prospects of Deep Oil and Gas

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Acta Geologica Sinica(English Edition) 2022年第3期96卷 742-756页

作者： hao fang Key Laboratory of Deep Oil and Gas China University of Petroleum(East China)Qingdao 266580China School of Geosciences China University of Petroleum(East China)Qingdao 266580China

With the deepening of oil and gas exploration,the importance of depth is increasingly *** risk of preservation of storage space in deep reservoirs is greater than that in shallow and medium *** layers mean older strata,more complex structural evolution and more complex hydrocarbon accumulation processes,and even adjustment and transformation of oil and gas *** paper systematically investigates the current status and research progress of deep oil and gas exploration around the world and looks forward to the future research focus of deep oil and *** the deep,especially the ultra-deep layers,carbonate reservoirs play a more important role than clastic ***,fault-karst and dolomite reservoirs are the main types of deep and ultra-deep *** common feature of most deep large and medium-sized oil and gas reservoirs is that they formed in the early with shallow *** activity and evolution of trap highs are the main ways to cause physical adjustment of oil and gas *** oil cracking and thermochemical sulfate reduction(TSR)are the main chemical modification effects in the ***-scale high-quality dolomite reservoirs is the main direction of deep oil and gas *** identification of oil and gas charging,adjustment and reformation processes is the key to understanding deep oil and gas ***-precision detection technology and high-precision dating technology are an important guarantee for deep oil and gas research.

关键词： deep oil and gas carbonate reservoir main accumulation period reservoir adjustment and reconstruction enrichment mechanism

Population Growth and Development of the Nipa Palm Hispid Beetle,Octodonta nipae(Maulik)(Coleoptera:Chrysomelidae)at Constant Temperatures

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Population Growth and Development of the Nipa Palm Hispid Be...