SPECIES-SPECIFIC MTCOI MAKER FOR RAPID DIAGNOSE OF INVASIVE SPEICES,FRANKLINIELLA OCCIDENTALIS (PERGANDE)
会议名称：《International Congress on Biological Invasions》
会议日期：2009年
学科分类：09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治]
摘 要：The western flower thrips,Frankliniella occidentalis(Pergande)(Thysanoptera: Thripidae),is an invasive species and currently occurs only in a few areas in China.A quick and correct and developmental-stage non-limiting method of the identification of the western flower thrips is essential for preventing the spread of this thrips. Morphological identification of thrips is performed on adults and to a lesser extent on second-stage larvae *** identification,however,can be easily followed by a non-thrips-specialist with a little training.A species-specific marker within the mitochondrial cytochrome oxidase I(COI) sequence for *** was developed in the present *** specific primers amplified a single band of 340 *** specificity of the primers was assessed using 28 other thrips species commonly occurring in *** *** specimens were detected,and no cross reactions with other thrips species were *** method was tested on single egg,first- and second-stage larvae,pre-pupae,pupae and adults,and proved to be applicable for all these ***,the method was used to clarify the distribution of the western flower thrips in Beijing metro area *** results suggested that the species-specific mtCOI marker without gene sequencing analysis is useful in the early detection and rapid identification of ***,and makes it possible for diagnostic testing and routine screening of samples at ports of entry and preventing new incursions and further damage of this thrips in China.