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Identification and expression analysis of the Pbr MLO gene family in pear, and functional verification of PbrMLO23

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Journal of Integrative Agriculture 2021年第9期20卷 2410-2423页

作者： GUO Bing-bing LI Jia-ming LIU Xing QIAO Xin Musana Rwalinda fabrice WANG Peng ZHANG Shao-ling WU Ju-you Center of Pear Engineering Technology Research/State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095P.R.China School of Life Sciences Nanjing UniversityNanjing 210023P.R.China

Mildew resistance locus O(MLO) is a plant-specific gene family that plays an important role in the growth and development of plants and their interactions with the environment. However, the available information on this gene family in pear is limited. Here, 24 PbrMLO genes were identified and divided into five subfamilies(I, II, III, IV and V). Wholegenome duplication(WGD) and dispersed duplication contributed to the expansion of the PbrMLO family. In addition, gene expression analysis revealed that PbrMLO genes were distributed in various pear tissues, suggesting their diverse functions. We selected PbrMLO23 for further functional analysis. Expression profile analysis by qRT-PCR showed that PbrMLO23 was highly expressed in pollen. Subcellular localization analysis showed that PbrMLO23 was located on the plasma membrane. When the expression level of PbrMLO23 was knocked down by using antisense oligonucleotides, pollen tube lengths increased, indicating that PbrMLO23 plays a functional role in inhibiting pollen tube growth. In summary, these results provide evolutionary insight into PbrMLO and its functional characteristics and lay a foundation for further analysis of the functions of PbrMLO members in pear.

关键词： MLO pear gene expression pollen tube

Proteomics as a tool to gain next level insights into photo-crosslinkable biopolymer modifications

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Bioactive Materials 2022年第11期7卷 204-220页

作者： Nele Pien fabrice Bray Tom Gheysens Liesbeth Tytgat Christian Rolando Diego Mantovani Peter Dubruel Sandra Van Vlierberghe Ghent University Polymer Chemistry and Biomaterials Research GroupCentre of Macromolecular Chemistry(CMaC)Krijgslaan 281 S4bis9000 GentBelgium Laval University Laboratory for Biomaterials and BioengineeringCRC-IPavillon PouliotQuebec G1V 0A6Canada Univ.Lille CNRSUSR 3290MSAPMiniaturisation pour la Synthese l’Analyse et la ProteomiqueF-59000LilleFrance

The distribution of photo-crosslinkable moieties onto a protein backbone can affect a biomaterial’s crosslinking behavior, and therefore also its mechanical and biological properties. A profound insight in this respect is essential for biomaterials exploited in tissue engineering and regenerative medicine. In the present work, photo-crosslinkable moieties have been introduced on the primary amine groups of: (i) a recombinant collagen peptide (RCPhC1) with a known amino acid (AA) sequence, and (ii) bovine skin collagen (COL BS) with an unknown AA sequence. The degree of substitution (DS) was quantified with two conventional techniques: an ortho-phthalic dialdehyde (OPA) assay and ^(1)H NMR spectroscopy. However, neither of both provides information on the exact type and location of the modified AAs. Therefore, for the first time, proteomic analysis was evaluated herein as a tool to identify functionalized AAs as well as the exact position of photo-crosslinkable moieties along the AA sequence, thereby enabling an in-depth, unprecedented characterization of functionalized photo-crosslinkable biopolymers. Moreover, our strategy enabled to visualize the spatial distribution of the modifications within the overall structure of the protein. Proteomics has proven to provide unprecedented insight in the distribution of photo-crosslinkable moieties along the protein backbone, undoubtedly contributing to superior functional biomaterial design to serve regenerative medicine.

关键词： (Photo-crosslinkable)biomaterials Chemical modifications Biomaterial characterization Proteomics Localization of functionalizations

Smoothelin,a new marker to determine the origin of liver fibrogenic cells

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World Journal of Gastroenterology 2013年第48期19卷 9343-9350页

作者： Sébastien Lepreux Christelle Guyot fabrice Billet Chantal Combe Charles Balabaud Paulette Bioulac-Sage Alexis Desmoulière CHU Bordeaux Hopital Pellegrin Service d’Anatomie Pathologique F-33076 Bordeaux France U889 and Université Victor Segalen Bordeaux 2 F-33076 Bordeaux France Division of Clinical Pharmacology and Toxicology Department of Medicine University Hospital Zurich 8006 Zurich Switzerland Facultés de Médecine et de Pharmacie Université de Limoges EA 6309 F-87025 LimogesFrance

AIM: To explore this hypothesis that smooth muscle cells may be capable of acquiring a myofibroblastic phenotype, we have studied the expression of smoothelin in fibrotic conditions.

关键词： Smooth muscle cells, Myofibroblasts, α -smooth muscle actin, Smoothelin, Fibrosis/cirrhosis, Hepatocellular carcinoma

Modern post-mortem imaging:an update on recent developments

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Forensic Sciences Research 2017年第2期2卷 52-64页

作者： Silke Grabherr Coraline Egger Raquel Vilarino Lorenzo Campana Melissa Jotterand fabrice Dedouit University Centre of Legal Medicine Lausanne-Geneva LausanneSwitzerland Service of Legal Medicine Central Institute of HospitalsSionSwitzerland

Modern post-mortem investigations use an increasing number of digital imaging methods,which can be collected under the term“post-mortem imaging”.Most methods of forensic imaging are from the radiology field and are therefore techniques that show the interior of the body with technologies such as X-ray or magnetic resonance *** digitally image the surface of the body,other techniques are regularly applied,***-dimensional(3D)surface scanning(3DSS)or ***’s most frequently used techniques include post-mortem computed tomography(PMCT),post-mortem magnetic resonance imaging(PMMR),post-mortem computed tomographic angiography(PMCTA)and 3DSS or *** of these methods has specific advantages and ***,the indications for using each method are *** PMCT gives a rapid overview of the interior of the body and depicts the skeletal system and radiopaque foreign bodies,PMMR allows investigation of soft tissues and parenchymal *** is the method of choice for viewing the vascular system and detecting sources of ***,none of those radiological methods allow a detailed digital view of the body’s surface,which makes 3DSS the best choice for such a *** 3D surface scanners are not available,photogrammetry is an *** review article gives an overview of different imaging techniques and explains their applications,advantages and *** hope it will improve understanding of the methods.

关键词： Forensic science forensic imaging post-mortem radiology post-mortem computed tomography post-mortem magnetic resonance imaging 3D scanning post-mortem angiography photogrammetry

Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology

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Avian Research 2019年第1期10卷 89-100页

作者： Ségolène Humann?Guilleminot Pierre Blévin Antonin Azou?Barré Agathe Yacoumas Geir Wing Gabrielsen Olivier Chastel fabrice Helfenstein Laboratory of Evolutionary Ecophysiology Institute of BiologyUniversity of Neuchatel Centre d'Etudes Biologiques de Chizé(CEBC) UMR 7372-CNRSUniversité de La Rochelle Norwegian Polar Institute Fram Centre

Background:Collecting and studying live sperm is central to many important fields of ***,a simple method to collect live sperm is lacking in wild seabird ***,we describe a non?invasive method to collect viable sperm samples based on a simple massage technique applied to male Black?legged Kittiwakes(Rissa tridactyla).Methods:We studied a colony breeding at Kongsfjorden,Svalbard and successfully obtained sperm samples from 32 *** a subset of samples(n = 12 males),we compared the suitability of several extenders(0.9% NaCl,PBS,Earle's balance salt solution,Dulbecco's modified Eagle medium) in maintaining sperm alive long enough for *** another 18 ejaculates,we conducted computer assisted sperm analyses using the CASA plugin for *** provide details about the settings to be used for such ***,droplets from 20 ejaculates were smeared on glass slides and preserved with formalin to characterize sperm morphology in terms of total sperm length,sperm head length,midpiece length and flagellum length,and percentage of abnormal ***:With this method and under field conditions,we were able to obtain sufficient amounts of live sperm to assess traits related to sperm quality(*** morphology,percentage of motile sperm,sperm velocity).We found that two extenders,Earle's balanced salt solution and Dulbecco modified Eagle's medium,yielded similarly good ***,we investigated whether specific behaviours were associated with successful sperm collection and whether sperm collection success depended on how long before laying sperm collection was ***,we provide mean values for sperm morphology,sperm swimming ability and percentage of motile sperm,which may prove useful for future comparative analyses,and we report high levels of sperm abnormality and within?ejaculate variation in sperm ***:We discuss the high percentage of abnormal sperm and high within?ejaculate variation in sperm

关键词： Black-legged Kittiwakes Sperm Spermatozoa Semen collection Non?invasive method Larids Sperm velocity Sperm morphology Abnormal sperm

Nickel phosphonate MOF as efficient water splitting photocatalyst

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Nano Research 2021年第2期14卷 450-457页

作者： Pablo Salcedo-Abraira Sergio M.F.Vilela Artem A.Babaryk Maria Cabrero-Antonino Pedro Gregorio fabrice Salles Sergio Navalon Hermenegildo Garcia Patricia Horcajada IMDEA Energy Advanced Porous Materials Unit(APMU)Avda.Ramon de Ia Sagra 3E 28935 MostolesMadridSpain Deparrtamento de Quimica Inorginica I.Fac.CC.Quimicas Universidad Complutense de MadridAvda.Complutense s/n28040 MadridSpain Departamento de Quimica and Instituto de Tecnologia Quimica(CSIC-UPV) Universitat Politecnica de ValenciaC/Camino de Veras/n46022 ValenciaSpain Institut Charles Gerhardt Montpellier UMR 5253 CNRS UM ENSCMUniversite MontpellierPlace E.Bataillon34095 Montpellier Cedex 05France

A novel microporous two-dimensional(2D)Ni-based phosphonate metal-organic framework(MOF;denoted as IEF-13)has been successfully synthesized by a simple and green hydrothermal method and fully characterized using a combination of experimental and computational *** resolution by single-crystal X-ray diffraction reveals that IEF-13 crystallizes in the triclinic space group Pi having bi-octahedra nickel nodes and a photo/electroactive tritopic phosphonate ***,this material exhibits coordinatively unsaturated nickel(II)sites,free-P0_(3)H_(2)and-P0_(3)H acidic groups,a C0_(2)accessible microporosity,and an exceptional thermal and chemical ***,its in-deep optoelectronic characterization evidences a photoresponse suitable for *** this sense,the photocatalytic activity for challenging H_(2)generation and overall water splitting in absence of any co-catalyst using UV-Vis irradiation and simulated sunlight has been evaluated,constituting the first report for a phosphonate-MOF ***-13 is able to produce up to 2,200 fimol of H_(2)per gram using methanol as sacrificial agent,exhibiting stability,maintaining its crystal structure and allowing its *** more,170μmol of H_(2)per gram were produced using IEF-13 as photocatalyst in the absence of any co-catalyst for the overall water splitting,being this reaction limited by the 0_(2)*** present work opens new avenues for further optimization of the photocatalytic activity in this type of multifunctional materials.

关键词： metal-organic framework phosphonates photocatalysis water splitting

Spatio-Temporal Land Cover Analysis and the Impact of Land Cover Variability Indices on Land Surface Temperature in Greater Accra, Ghana Using Multi-Temporal Landsat Data

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Journal of Geographic Information System 2022年第3期14卷 240-258页

作者： Aarthi Aishwarya Devendran fabrice Banon Department of Built Environment and Energy Technology Linnaeus University V&#228 xjö Sweden Ecole Africaine des Métiers de l’Architecture et de l’Urbanisme Lomé Togo

Urbanization, as a result of anthropogenic activities, reduces the vegetated and green spaces and thereby increases the impervious surfaces in cities. This in turn increases the surface temperature of cities when compared to the rural regions resulting in the formation of urban heat island. Especially, in under-developed countries, it is very crucial to obtain timely and accurate information on the urban trend and its development due to the higher increasing rate of population growth and lack of infrastructural facilities and regulations to mitigate the adverse consequences of urbanization. The current study analyzes the urban development of Greater Accra, Ghana using Landsat 7 dataset acquired in 2002, 2013, and 2020. Further, the influence of urban growth on the land surface temperature (LST) and land cover variability (LCV) indices including NDVI (Normalized Difference Vegetation Index), NDBI (Normalized Difference Built-Up Index), and NDWI (Normalized Difference Water Index) is identified during the study periods. Results suggest that the area of built-up quadrupled between 2002 and 2020 in the study region. Shannon’s entropy-based analysis suggests that the urban development in the region is heterogeneous or dispersive in nature. Further, the regression analysis highlights a strong positive relation between LST and NDBI (0.755) and a negative relation is found associated between LST and NDVI (0.4417) and LST and NDWI (0.76). Results of the study could be useful to design sustainable urban socio-economic and environmental planning policies in the study region.

关键词： Land Surface Temperature Urbanization Land Cover Variability Indices Landsat 7 ETM Greater Accra

Adaptive Performance Improvement of Fiber Bragg Grating in Radio over Fiber System

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Journal of Computer and Communications 2016年第3期4卷 1-6页

作者： fabrice Mfuamba Kabonzo Yunfeng Peng School of Computer and Communication Engineering University of Science and Technology Beijing China

The combination of Radio Frequency and Optical Fiber has resulted high capacity transmission at lower costs components and makes Radio over Fiber as a current trend of large broadband communication. In Fiber optics field, the use of Fiber Bragg Grating (FBG) was been proposed in recent research with different purpose of uses. However, the compensation of dispersion method of Fiber Bragg Grating (FBG) can boost significantly the system performance. This paper investigates the performance capacity improvement of adaptive Radio over Fiber system. The system design was performed using OptiSystem 7.0 software, which 10 Gb/s Non Return to Zero (NRZ) signal was launched into 50 Km Universal Mode Fiber and Fiber Bragg Grating was used as a compensator of dispersion before frequency up conversion. Therefore, the system performances were investigated by comparing the Bit Error Rate (BER) and Q-factors of Positive Intrinsic Negative (PIN) and Ultrafast Avalanche Photodiode (APD) as optical receivers. The Eye diagram analyzer showed acceptable improvement due to use of Fiber Bragg Grating as a compensator of dispersion.

关键词： Radio over Fiber (RoF) Fiber Bragg Grating (FBG) Dispersion Compensating Fiber (DCF) Positive Intrinsic Negative (PIN) Ultrafast Avalanche Photodiode (APD)

硅光电倍增器的变温伏安特性及水汽凝结对伏安特性的影响

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半导体光电 2018年第3期39卷 337-340页

作者：张国青 fabrice RETIERE 西安工程大学理学院物理系西安710048 加拿大粒子加速器中心(TRIUMF) 温哥华V6T2A32

为了研究硅光电倍增器(SiPM)在低温下能否正常工作,选取了两种典型的SiPM,通过液氮制冷方式,对SiPM在不同温度下的反向伏安特性进行了研究。结果显示,不同SiPM的过偏压范围(V_B^V_b)随温度的变化差别很大,并且微量水蒸气凝结仅对未封装... 详细信息

为了研究硅光电倍增器(SiPM)在低温下能否正常工作,选取了两种典型的SiPM,通过液氮制冷方式,对SiPM在不同温度下的反向伏安特性进行了研究。结果显示,不同SiPM的过偏压范围(V_B^V_b)随温度的变化差别很大,并且微量水蒸气凝结仅对未封装的SiPM伏安特性的V_b^V_B段有明显影响。分析实验结果得出,SiPM正常工作电压的范围在很大程度上受到衬底材料中缺陷和陷阱浓度的影响。在低温下工作的SiPM,要求其衬底材料中缺陷和陷阱的浓度更低。在进行SiPM的低温应用和测量时,应密切监视偏压加在V_b^V_B区间时,器件的电流是否有变化,而不能只观察击穿之前SiPM的漏电情况。

关键词：硅光电倍增器多像素光子计数器伏安特性水汽凝结