Identification and expression analysis of the Pbr MLO gene family in pear, and functional verification of PbrMLO23

作     者：GUO Bing-bing LI Jia-ming LIU Xing QIAO Xin Musana Rwalinda FABRICE WANG Peng ZHANG Shao-ling WU Ju-you GUO Bing-bing;LI Jia-ming;LIU Xing;QIAO Xin;Musana Rwalinda FABRICE;WANG Peng;ZHANG Shao-ling;WU Ju-you

作者机构：Center of Pear Engineering Technology Research/State Key Laboratory of Crop Genetics and Germplasm EnhancementCollege of HorticultureNanjing Agricultural UniversityNanjing 210095P.R.China School of Life SciencesNanjing UniversityNanjing 210023P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第9期

页      面：2410-2423页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 090201[农学-果树学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (31772276) the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China the China Postdoctoral Science Foundation (2017M620213) 

主　　题：MLO pear gene expression pollen tube 

摘      要：Mildew resistance locus O(MLO) is a plant-specific gene family that plays an important role in the growth and development of plants and their interactions with the environment. However, the available information on this gene family in pear is limited. Here, 24 PbrMLO genes were identified and divided into five subfamilies(I, II, III, IV and V). Wholegenome duplication(WGD) and dispersed duplication contributed to the expansion of the PbrMLO family. In addition, gene expression analysis revealed that PbrMLO genes were distributed in various pear tissues, suggesting their diverse functions. We selected PbrMLO23 for further functional analysis. Expression profile analysis by qRT-PCR showed that PbrMLO23 was highly expressed in pollen. Subcellular localization analysis showed that PbrMLO23 was located on the plasma membrane. When the expression level of PbrMLO23 was knocked down by using antisense oligonucleotides, pollen tube lengths increased, indicating that PbrMLO23 plays a functional role in inhibiting pollen tube growth. In summary, these results provide evolutionary insight into PbrMLO and its functional characteristics and lay a foundation for further analysis of the functions of PbrMLO members in pear.