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Research methods in complementary and alternative medicine： an integrative review

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Journal of Integrative Medicine 2018年第1期16卷 6-13页

作者： Fabiana de Almeida andrade Caio fabio Schlechta Portella Anhembi Morumbi University Sao Paulo 01423-001 Brazil department of Naturology Anhembi Morumbi University Sao Paulo 06730-000 Brazil

The scientific literature presents a modest amount ot evidence m the use or complementary ana al[erna- tire medicine （CAM）. On the other hand, in practice, relevant results are common. The debates among CAM practitioners about the quality and execution of scientific research are important, Therefore, the aim of this review is to gather, synthesize and describe the differentiated methodological models that encompass the complexity of therapeutic interventions. The process of bringing evidence-based medicine into clinical practice in CAM is essential for the growth and strengthening of complementary medicines worldwide.

关键词： Research methodology Complementary medicine Alternative medicine Complementary therapies Comparative effectiveness research Outcome assessment Nonlinear dynamics Evaluation studies

Microbial enzymatic stoichiometry and the acquisition of C, N,and P in soils under different land-use types in Braziliansemiarid

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Soil Ecology Letters 2023年第3期5卷 101-108页

作者： Erika Valente de Medeiros Érica de Oliveira Silva Gustavo Pereira Duda Mario andrade Lira Junior Uemeson Josédos Santos Claude Hammecker Diogo Paes da Costa fabio Fernando Araujo Arthur Prudêncio de Araujo Pereira Lucas William Mendes Ademir Sergio Ferreira Araujo Laboratory of Microbiology and Enzymology-LEMA Federal University of Agreste PernambucoGaranhuns 55292-270Brazil Federal Rural University of Pernambuco Rua Dom Manuel de Medeiross/nDois Irmãos52171-900RecifeBrazil Soil-Agrosystem-Hydrosystem Interaction Lab-LISAH Place Pierre Viala234060 MontpellierFrance Universidade do Oeste Paulista Presidente PrudenteSPBrazil departamento de Ciência Do Solo Universidade Federal Do CearáFortalezaCEBrazil Center for Nuclear Energy in Agriculture University of Sao PauloPiracicabaSPBrazil Soil Quality Lab Agricultural Science CenterFederal University of PiauíTeresinaPI 60049-550Brazil

This study hypothesized that different land-use affect the microbial enzymatic stoichiometry and C-,N-,and P-acquisition in Brazilian semiarid ***,the enzymesβ-glucosidase(C-acquiring enzyme),urease(N-acquiring enzyme),and acid phosphatase(P-acquiring enzyme)were assessed in soil samples collected at 0−5 and 5−10 cm depth from a tropical dry forest,a protected area with Angico,a protected area with Ipê,scrub area,and an agricultural area with *** values of C-,N-,and P-acquiring enzymes were used to calculate the enzymatic C:N,C:P,and N:P *** values of C:P and N:P ratios were higher at 0−5 cm depth,while no significant variation,between soil depth,was observed for C:N *** values of C-and N-acquiring enzymes were higher at 0−5 cm in tropical dry forest areas and Angico forest,*** all land use types,the values of vectors L and A were higher than 1°and 45°,*** study showed that both land-use and soil depth influence the enzymatic stoichiometry,showing higher values of C-and N-acquiring enzymes in native and protected forests at soil surface.

关键词： C:N:P stoichiometry tropical forests soil depth Luvisols

New translational and experimental insights into the role of proresolving lipid mediators in inflammatory bowel disease

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World Journal of Experimental Medicine 2022年第1期12卷 1-15页

作者： Lívia Bitencourt Pascoal Bruna Biazon Palma fabio Henrique Mendonça Chaim Marina Moreira de Castro Tiago andrade Damázio Ana Paula Menezes de Freitas Franceschini Marciane Milanski Lício AugustoVelloso Raquel Franco Leal Inflammatory Bowel Disease Research Laboratory Colorectal Surgery UnitDepartment of SurgerySchool of Medical SciencesUniversity of CampinasCampinas 13083-878São PauloBrazil Laboratory of Metabolic Disorders School of Applied SciencesUniversity of CampinasCampinas 13083-878São PauloBrazil Laboratory of Cell Signaling School of Medical SciencesUniversity of CampinasCampinas 13083-864São PauloBrazil

The resolution of inflammation is an active process,guided by specialized proresolution lipid mediators(SPMs).These mediators originate from polyunsaturated fatty acids,such as *** evidence suggests that the beneficial effects attributed to omega-3 are,at least in part,the result of the immunomodulatory action of the SPMs,which act systemically by overcoming inflammation and repairing tissue damage,without suppressing the immune *** studies suggest that an imbalance in the synthesis and/or activity of these compounds may be associated with the pathogenesis of several inflammatory conditions,such as inflammatory bowel disease(IBD).Thus,this review highlights the advances made in recent years with regard to the endogenous synthesis and the biological role of lipoxins,resolvins,protectins,and maresins,as well as their precursors,in the regulation of inflammation;and provides an update on the participation of these mediators in the development and evolution of IBD and the therapeutic approaches that these immunomodulating substances are involved in this context.

关键词： Pro-resolving lipid mediators Anti-inflammatory cytokines Omega-3 Chronic inflammation Pro-inflammatory cytokines Inflammatory bowel disease

Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia(Hemsl) *** leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 245-253页

作者： Bruno Rafael Barboza Bárbara Rafaela da Silva Barros Bárbara de Azevedo Ramos Maiara Celine de Moura Thiago Henrique Napoleao Maria Tereza dos Santos Correia Luana Cassandra Breitenbach barroso Coelho Iranildo José da Cruz Filho Ana Maria Souto Maior Túlio Diego da Silva Leylianne de Cássia Rodrigues Nerys Edson Renan Barros de Santana Cláudia Sampaio de andrade Lima Virgínia Maria Barros de Lorena Cristiane Moutinho Lagos de Melo department of Antibiotics Biosciences Centre Federal University of Pernambuco department of Biochemistry Biosciences Centre Federal University of Pernambuco Center for Strategic Techmologies of the Northeast department of Biophysics Biosciences Centre Federal University of Pernambuco Aggeu Magalhaes Institute IAM/FIOCRUZ

Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal ***:The saline extract was prepared,with NaCl(0.15 M),by constant stirring of the dried and pulverized leaves,followed by volume reduction by *** extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was *** antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice ***:Fifteen compounds were identified belonging to two main classes terpenoids and *** extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of ***,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these ***:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.

关键词： Tithonia diversifolia Extract Antioxidant Antifungal properties Immunostimulation

Biodiesel Production from Waste Cooking Oil by Enzymatic Catalysis Process

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Journal of Chemistry and Chemical Engineering 2013年第10期7卷 993-1000页

作者： Ignacio Contreras andrade fabio Emiro Sierra Vargas Carlos Alberto Guerrero Fajardo department of Biology and Chemistry University of Sinaloa Culiacan 80007 Mexico department of Chemistry National University of Colombia Bogota 11001 Colombia

Biodiesel is an excellent option for reducing dependence on fossil fuels with environmental advantages by reducing hazardous emissions. The enzymatic transesterification has attracted the attention of researchers in the last decade and the advantages of enzymatic catalysis show that the production of biodiesel by this route has good potential, mainly because it is friendly environment. For biodiesel, production process by enzyme catalysis is chosen the response surface methodology. It is an experimental strategy to find the best operating conditions oftransesterification reaction to improve the biodiesel quality. The Process has three variables： temperature, molar ratio oil-alcohol and catalyst quantity. The process was monitored by GC-FID （gas chromatography with flame ionization detector）. The yield of the transesterification reaction by enzymatic catalysis decreases with increasing temperature, and may be due to inactivation of the enzyme by denaturation at temperatures above 50 ℃. The second-order design used was the ＂CDC （central design composition）＂ which produced a maximum yield of 95.5% in the transesterification reaction by enzymatic catalysis obtained at a temperature of 45 ℃, molar ratio methanol：oil of 8：1 and a catalyst loading of 8% wt.

关键词： Biodiesel waste cooking oil transesterification reaction enzymatic catalysis biofuel

Computational Simulation to Predict the Syngas Composition Produced during Charcoal Gasification

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Journal of Chemistry and Chemical Engineering 2014年第9期8卷 889-897页

作者： Ignacio Contreras-andrade Santiago Ramirez-Rubio Jonathan Parra-Santiago fabio Sierra-Vargas Carlos Alberto Guerrero-Fajardo Doctor Chemical Engineering Autonomous University of Sinaloa Culiacan Rosales 80040 Mexico department of Aeronautical Engineering San Buenaventura University Bogota 11001 Colombia Chemical Engineering (C) National University of Colombia Bogota 11001 Colombia Oepartment of Mechanical Engineering National University of Colombia Bogota 11001 Colombia Chemical Engineering and Mechanical Master in Environmental Engineering PhD in Chemical Engineering National Universityof Colombia Bogota 11001 Colombia

Charcoal gasification could mitigate the energetic problems in the rural zones since these regions have considerable amounts of wood, which is the base of such a fuel available. This paper presents some CFD （computational fluid dynamic） predictions of the experimental results obtained from the fixed bed gasification of charcoal made in a pilot-scale downdraft reactor using air, which was designed and built by the Research Group in Clean development Mechanisms and Energy Management, from the National University of Colombia. The quality of the syngas obtained from the process was evaluated through the CO and CO2 percentages measured in its composition. The performance at various air flow rates （measured at the system entrance, through an analog flow meter） is evaluated with the help of 11 thermocouples, which give the information to create a temperature profile, and three load cells to measure the solid fuel conversion rate. To simulate the process, the information from temperature profile, charcoal proximate analysis, air flow meter and load cells were taken as inputs and the syngas composition was obtained as the result from the calculation. The domain was defined as 2D with an axis-symmetric description, using quads as mesh elements. The calculation and results were performed in a CFD commercial code widely used for this type of simulations： ANSYS FLUENT. The predictions made by the software were validated with the experimental results obtained in the laboratory.

关键词： Biomass charcoal fixed bed gasification syngas CFD.

Tetramethrin Identified in Fat Adjacent to Mammary Carcinoma in a Male Dog—Case Report

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Open Journal of Pathology 2012年第3期2卷 102-105页

作者： Fernanda C.Figueiroa Paulo Ricardo O.Bersano fabio Henrique E.andrade Noeme S.Rocha School of Veterinary Medicine and Animal Science Sao Paulo State University(UNESP)BotucatuBrazil Maranhao State University(UEMA) Sao LuisBrazil

Canine mammary tumors (CMT) are very common in female dogs, representing approximately 50% of all tumors in this species, but are highly uncommon in male dogs and are primarily related to hyperestrogenism. There is considerable scientific interest in the possible role of environmental contaminants in the etiology of mammary tumors, specifically in relation to synthetic chemical substances released into the environment to which living beings are either directly or indirectly exposed. In this study, the presence of pyrethroid insecticide was observed in adipose tissue adjacent to canine mammary tumors. High Precision Liquid Chromatography—HPLC was adapted to detect and identify environmental contaminants in adipose tissue adjacent to malignant mammary tumor in one male dog, Akita, 12 years old. After surgery, the mass was carefully examined for malignant neoplastic lesions. Five grams of adipose tissue adjacent to the tumor was collected to detect environmental contaminants. The pyrethroid was identified as tetramethrin, at 0.20 μg/g. This is the first report in which the environmental contaminant level was detectable in adipose tissue of this male dog with a malignant mammary tumor, by HPLC. Results suggest the possible involvement of pyrethroid in the canine mammary tumor carcinogenesis since this animal did not present any other tumor that could cause hyperestrogenism.

关键词： Tetramethrin Mammary Tumor Male Dog Environmental Contaminants

Comparison of Three Experimental Models for Rat Osteoarthritis Induction

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Journal of Biosciences and Medicines 2016年第12期4卷 62-69页

作者： Henrique Ribeiro Rodrigues-Neto Edilson Ferreira andrade-Junior denilson Jose Silva Feitosa-Junior André Lopes Valente Thiago Cesar Xavier Bianca Caroline do Nascimento Alho Renan Kleber Costa Teixeira fabio Vidal Moriya Rui Sérgio Monteiro de Barros department of Orthopaedics and Traumatology Porto Dias Hospital Bel&eacute m Brazil Experimental Surgery Laboratory Par&aacute State University Bel&eacute m Brazil

Background: Osteoarthritis is a slowly progressive and debilitating disease with high prevalence in adult population. Knee is one of the joints most affected by this disorder. There are several models for animals’ osteoarthritis induction, however it is not identified any paper that compares these techniques. The present study was aimed to define the most appropriate model for rats osteoarthritis induction. Material and Methods: 40 Wistar rats were distributed into 4 groups of 10 animals each: normality group (NG);meniscectomy group (MG);quinolone group (QG) and iodoacetate group (IG). Radiographic images of the rat’s knees were analyzed as well as the amount of chondrocytes in the epiphyseal and articular cartilage. Results: In the radiographic analysis, there was a low correlation between the raters. Regarding the amount of chondrocytes in the epiphyseal cartilage, it was noticed that the IG and QG groups had fewer chondrocytes than NG, in contrast to MG that reported similar results to normality (p > 0.05). There was no significant difference between IG and QG groups (p > 0.05). Regarding the amount of chondrocytes in articular cartilage, it was noticed that the IG group showed fewer chondrocytes than NG (p 0.05). There was no significant difference between QG and MG groups (p > 0.05). Conclusion: Intraarticular injection of iodoacetate in rats is the model with greatest effect on reduction of chondrocytes amount.

关键词： Osteoarthritis Knee Joint Experimental Arthritis Animal Disease Models Rats

dengue in renal transplant recipients: Clinical course and impact on renal function

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World Journal of Transplantation 2017年第1期7卷 57-63页

作者： Paula Frassinetti Castelo Branco Camur?a Fernandes Reed AndréSiqueira Evelyne Santana Gir?o Rainne AndréSiqueira Márcia Uchoa Mota Leyla Castelo Branco Fernandes Marques Silvana Cristina Albuquerque andrade Wilson Mendes barroso S?nia Leite Silva Bruno Gomes Rodrigues dos Santos Claúdia Maria Costa de Oliveira Kidney Transplant Unit Hospital Universitário Walter Cantídio Universidade Federal do Ceará department of Nephrology of Universidade Estadual do Ceará Universidade Estadual do Ceará

AIM To present clinical characteristics from renal transplant recipients with dengue fever and its impact on graft *** We retrospectively evaluated 11 renal transplant recipients(RTR) with dengue infection confirmed by laboratory test, between January 2007 and July 2012, transplanted in the Renal Transplant Center of Walter Cantídio University Hospital from Federal University of Ceará. RESULTS Positive dengue serology(IgM) was found in all patients. The mean time between transplant and dengue infection was 43 mo. Fever was presented in all patients. Nine patients presented with classical dengue and two(18%) with dengue hemorrhagic fever. All cases had satisfactory evolution with complete recovery of the symptoms. The time for symptom resolution varied from 2 to 20 d, with an average of 9 d. An increase of creatinine after the infection was observed in three(27.2%) patients with no clinically impact on the kidney graft function. CONCLUSION RTR with dengue infection seems to have a clinical presentation and evolution similar to those seen in the general population, with no long-term damage to patient and to the graft.

关键词： Kidney Renal Transplant dengue Clinical Brazil