检索结果-南通市图书馆

Influence of chelator and near-infrared dye labeling on biocharacteristics of dual-labeled trastuzumab-based imaging agents

引用

Chinese Journal of Cancer Research 2016年第3期28卷 362-369页

作者： Xuejuan Wang melissa B Aldrich Zhi Yang Nina Zhou Qing Xie Chen Liu eva Sevick-muraca Key Laboratory of Carcinogenesis and Translational Research(ministry of Education/Beijing) Department of Nuclear MedicinePeking University Cancer Hospital&InstituteBeijing 100142China Center for molecular Imaging the Brown Foundation Institute of Molecular MedicineUniversity of Texas Health Science Center at HoustonHouston TX 77030USA

Objective： To investigate the effect of fluorescent dye labeling on the targeting capabilities of 111 In- （DTPA）.-trastuzumab-（IRDye 800）m. methods： Trastuzumab-based conjugates were synthesized and conjugated with diethylenetriaminepentaacetic acid （DTPA） at molar ratios of I, 2, 3 and 5 and with a fluorescent dye （IRDye 800CW） at molar ratios of 1, 3 and 5. Immunoreactivity and internalization were assessed on SKBR-3 cells, overexpressing human epidermal growth factor receptor 2. The stability in human serum and phosphate-buffered saline （PBS） was evaluated. The biodistribution of dual-labeled conjugates was compared with that of 111In-（DTPA）2-trastuzumab in a SKBR-3 xenograft model to evaluate the effect of dye-to-protein ratio. Results： All trastuzumab-based conjugates exhibited a high level of chemical and optical purity. Flow cytometry results showed that increasing dye-to-protein ratios were associated with decreased immunoreactivity. Stability studies revealed that the conjugate was stable in PBS, while in human serum, increased degradation and protein precipitation were observed with increasing dye-to-protein ratios. At 4 h, the percentages of internalization of dual-labeled conjugates normalized by dye-to-protein ratio （m） were 24.88%±2.10%, 19.99%±0.59%, and 17.47%±1.26% for ＂m＂ equal to 1, 3, and 5, respectively. A biodistribution study revealed a progressive decrease in tumor uptake with an increase in the dye-to-protein ratios. The liver, spleen and kidney showed a marked uptake with increased dye-to-protein ratios, particularly in the latter. Conclusions： With non-specific-site conjugation of the fluorescent dye with a protein based on imaging agent, the increase in dye-to-protein ratios negatively impacted the immunoreactivity and stability, indicating a reduced tumor uptake.

关键词： Influence near infrared dye trastuzumab human epidermal growth factor receptor 2 dual-labeled

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gastrointestinal adverse events associated with immune checkpoint inhibitor therapy

引用

Gastroenterology Report 2020年第1期8卷 25-30,I0001页

作者： eva Rajha Patrick Chaftari mona Kamal Julian maamari Christopher Chaftari Sai-Ching Jim Yeung Department of Emergency medicine The University of Texas MD Anderson Cancer CenterHoustonTXUSA Schoool of medicine Lebanese American UniversityByblosLebanon Biomedical Engineering College of EngineeringTexas A&M UniversityCollege StationTXUSA

Immunotherapy with checkpoint inhibitors has revolutionized cancer therapy and is now the standard treatment for several different types of cancer,supported by favorable outcomes and good ***,it is linked to multiple immune manifestations,referred to as immune-related adverse events(irAEs).These adverse events frequently affect the skin,colon,endocrine glands,lungs,and *** gastrointestinal system is one of the most commonly affected organ systems and is responsible for the most frequent emergency visits resulting from ***,because immune checkpoint inhibitors are a recent addition to our arsenal of cancer drugs,many health-care providers remain unfamiliar with the management of *** involved in the treatment of oncology patients who have received checkpoint inhibitors are currently encountering cases of abdominal pain,diarrhea,and other nonspecific symptoms that may be challenging to *** article reviews the gastrointestinal,hepatic,and pancreatic toxicities of checkpoint inhibitors and provides an approach to their diagnosis and recommended *** also highlights the management of irAEs according to their toxicity grading and specifically discusses the instances in which corticosteroids should be administered and/or the immune checkpoint inhibitors should be withheld.

关键词： immune checkpoint inhibitor immune-related adverse events colitis enterocolitis liver toxicity pancreatic toxicity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity

引用

Nano Research 2020年第4期13卷 1081-1089页

作者： marianna Barbalinardo Andrea Antosova marta Gambucci Zuzana Bednarikova Cristiano Albonetti Francesco Valle Paola Sassi Loredana Latterini(ISI) Zuzana Gazova eva Bystrenova C.N.R.-I.S.m.N via gobetti101bologna 40129italy Institute of experimental physics department of biophysicsslovak academy of sciencewatsonova 4704001kosiceslovakia department of chemistry biology and biotechnologyuniversity of perugiaperugia 06123italy consorzio interuniversitario per lo sviluppo dei sistemi a grande interfase(csgi) ismn-cnrbologna 40129italy department of medical and clinical biochemistry faculty of medicinesafarik universitykosice 04001slovakia

The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related *** influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of *** physico-chemical parameters play a role in the interaction of proteins and nanoparticles in solution,thus influencing the disaggregation of preformed *** have examined the influence of two kinds of metallic nanoparticles on lysozyme amyloid fibrils using a multi-technique approach and focalized their impact on cytotoxicity on human neuroblastoma cells(SH-SY5Y).In particular,fluorescence,infrared and circular dichroism spectroscopies,optical and atomic force microscopy experiments have been carried out;the results are analyzed to rationalize the effects of these complexes on neural cell *** is remarkable,that the fibrils in the presence of AuNPs,unlike fibrils alone or with AgNPs,do not generate a significant cytotoxic effect even at high concentration and an amyloid degradation effect is visible.

关键词： lysozyme amyloid fibrils nanoparticles spectroscopy toxicity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Linear maximum Likelihood Regression Analysis for Untransformed Log-Normally Distributed Data

引用

Open Journal of Statistics 2012年第4期2卷 389-400页

作者： Sara m. Gustavsson Sandra Johannesson Gerd Sallsten eva m. Andersson Department of Occupational and Environmental medicine Sahlgrenska University Hospital and Academy at University of Gothenburg Gothenburg Sweden

medical research data are often skewed and heteroscedastic. It has therefore become practice to log-transform data in regression analysis, in order to stabilize the variance. Regression analysis on log-transformed data estimates the relative effect, whereas it is often the absolute effect of a predictor that is of interest. We propose a maximum likelihood (mL)-based approach to estimate a linear regression model on log-normal, heteroscedastic data. The new method was evaluated with a large simulation study. Log-normal observations were generated according to the simulation models and parameters were estimated using the new mL method, ordinary least-squares regression (LS) and weighed least-squares regression (WLS). All three methods produced unbiased estimates of parameters and expected response, and mL and WLS yielded smaller standard errors than LS. The approximate normality of the Wald statistic, used for tests of the mL estimates, in most situations produced correct type I error risk. Only mL and WLS produced correct confidence intervals for the estimated expected value. mL had the highest power for tests regarding β1.

关键词： Heteroscedasticity maximum Likelihood Estimation Linear Regression model Log-Normal Distribution weighed Least-Squares Regression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Halomethoxybenzenes in air of the Nordic region

引用

Environmental Science and Ecotechnology 2023年第1期13卷 57-63页

作者： Terry Bidleman Agneta Andersson eva Brorstrom-Lunden Sonia Brugel Lars Ericson Katarina Hansson mats Tysklind Department of Chemistry UmeåUniversity90187UmeåSweden Department of Ecology&Environmental Science UmeåUniversity90187UmeåSweden Umeåmarine Science Centre UmeåUniversity90571HorneforsSweden IVL Swedish Environmental Research Institute(IVL)Aschebergsgatan 4441133GothenburgSweden

Halomethoxybenzenes(HmBs)are a group of compounds with natural and anthropogenic *** we extend a 2002e2015 survey of bromoanisoles(BAs)in the air and precipitation at Rå€o on the Swedish west coast and Pallas in Subarctic *** BAs data are reported for 2018 and 2019 and chlorinated HmBs are included for these and some previous years:drosophilin A methyl ether(DAmE:1,2,4,5-tetrachloro-3,6-dimethoxybenzene),tetrachloroveratrole(TeCV:1,2,3,4-tetrachloro-5,6-dimethoxybenzene),and pentachloroanisole(PeCA).The order of abundance of HmBs at Råo was SBAs>DAmE>TeCV>PeCA,whereas at Pallas the order of abundance was DAmE>SBAs>TeCA>*** lower abundance of BAs at Pallas reflects its inland location,away from direct marine ***-Clapeyron(CC)plots of log partial pressure(Pair)/Pa versus 1/T suggested distant transport at both sites for PeCA and local exchange for DAmE and *** were dominated by distant transport at Pallas and by both local and distant sources at Rå€*** between air and precipitation concentrations were examined by scavenging ratios,SR=(ng m^(-3))precip/(ng m^(-3))*** were higher at Pallas than Rå€o due to greater Henry's law partitioning of gaseous compounds into precipitation at colder *** is produced by terrestrial *** screened 19 fungal species from Swedish forests and found seven of them contained 0.01e3.8 mg DAmE per kg fresh *** suggest that the volatilization of DAmE from fungi and forest litter containing fungal mycelia may contribute to atmospheric levels at both sites.

关键词： Halomethoxybenzenes(HmBs) Bromoanisoles(BAs) Drosophilin A methyl ether(DAmE) Tetrachloroveratrole(TeCV) Atmospheric transport Sources

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

便携式微型发射光谱仪在金属工业中的应用(英文)

引用

冶金分析 2007年第5期27卷 8-13页

作者： TUSSET Victor LEmAIRE Olivier ZEImETZ eva DUPONT Alain C.R.m. LiègeBelgium

通过有证参考物质对便携式在线发射光谱仪在不同金属类别的快速成分控制的应用进行了考察。分析了大量的不同样品(低碳和超低碳钢,不锈钢,铝、镍和铜基样品),并通过采用参考的化学分析方法如ICP-OES,XRF,C和S的燃烧等等方法对结果进行... 详细信息

通过有证参考物质对便携式在线发射光谱仪在不同金属类别的快速成分控制的应用进行了考察。分析了大量的不同样品(低碳和超低碳钢,不锈钢,铝、镍和铜基样品),并通过采用参考的化学分析方法如ICP-OES,XRF,C和S的燃烧等等方法对结果进行了评价。对于低碳钢中的C,S和P的分析,通过特别的校正方法,采用新型的便携式光谱仪能获得与传统的固定式光谱仪相当的分析效果。本文也列举了多种非进样直接分析的相关应用实例。

关键词：便携式在线发射光谱仪快速成分控制金属

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Commentary of "skin grafting the vascular pedicle:a useful technique to avoid microvascular collapse in free tissue transfer for limb salvage"

引用

Plastic and Aesthetic Research 2019年第7期6卷 3-5页

作者： Francesco m.Egro eva Roy mario G.Solari Department of Plastic Surgery University of Pittsburgh Medical Center3550 Terrace Street 6B Scaife Hall PittsburghPittsburghPA 15261USA

We commend the authors on an excellent paper comparing the outcomes of microvascular transfers that utilized a skin graft for closure over the pedicle to a tension-free primary closure[1].

关键词： closure utilized microvascular

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Alternative Tapping Systems for RRIC 100 Clone from Opening

引用

材料科学与工程（中英文B版） 2013年第9期3卷 590-596页

作者： eva Herlinawati Kuswanhadi Sembawa Research Centre Indonesian Rubber Research Institute Palembang 30001 Indonesia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

携带污染物降解基因的可移动基因元件及其介导的生物修复

引用

应用生态学报 2011年第2期22卷 526-536页

作者：李慧周丽莎王亚菲 eva m.Top 张颖徐慧中国科学院沈阳应用生态研究所中国科学院陆地生态过程重点实验室沈阳110016 Department of Biological Sciences University of Idaho

可移动基因元件(mobile genetic elements,mGEs)在环境微生物群落中的水平转移是细菌基因组进化和适应特定环境压力的重要机制.在污染土壤和水体中接种携带具有降解基因mGEs的菌株后,随着mGEs的水平基因转移,可使降解基因转移至具有竞... 详细信息

可移动基因元件(mobile genetic elements,mGEs)在环境微生物群落中的水平转移是细菌基因组进化和适应特定环境压力的重要机制.在污染土壤和水体中接种携带具有降解基因mGEs的菌株后,随着mGEs的水平基因转移,可使降解基因转移至具有竞争性的土著微生物中并在其中表达,从而不必考虑供体菌在环境中是否能够长期存活.这种由可移动降解基因元件水平转移介导的生物修复为探索新的生物修复途径提供了可行性.本文重点综述了环境样品中携带降解基因mGEs的多样性及其在促进污染物降解过程中的重要作用,介绍了从环境样品中分离代谢mGEs的方法,并列举了在污染土壤、活性污泥、其他生物反应器等生态系统中mGEs水平转移的几个实例.

关键词：可移动基因元件(mGEs) 水平基因转移(HGT) 外源分离法生物修复

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

RIG-I immunotherapy overcomes radioresistance in p53-positive malignant melanoma

引用

Journal of molecular Cell Biology 2023年第1期15卷 13-26页

作者： Silke Lambing Yu Pan Tan Paraskevi Vasileiadou Stefan Holdenrieder Patrick müller Christian Hagen Stephan Garbe Rayk Behrendt martin Schlee Jasper G.van den Boorn eva Bartok marcel Renn Gunther Hartmann Institute of Clinical Chemistry and Clinical Pharmacology University Hospital BonnBonn 53127Germany Institute of Laboratory medicine German Heart CentreMunich 80636Germany Department of Radiation Oncology University Hospital BonnBonn 53127Germany Unit of Experimental Immunology Department of Biomedical SciencesInstitute of Tropical MedicineAntwerp 2000Belgium Institute of Experimental Haematology and Transfusion medicine University Hospital BonnBonn 53127Germany mildred Scheel School of Oncology BonnUniversity Hospital BonnMedical FacultyBonn 53127Germany

Radiotherapy induces DNA damage,resulting in cell cycle arrest and activation of cell-intrinsic death ***,the radioresistance of some tumour entities such as malignant melanoma limits its clinical *** innate immune sensing receptor retinoic acid-inducible gene I(RIG-I)is ubiquitously expressed and upon activation triggers an immunogenic form of cell death in a variety of tumour cell types including *** date,the potential of RIG-I ligands to overcome radioresistance of tumour cells has not been ***,we demonstrate that RIG-I activation enhanced the extent and immunogenicity of irradiation-induced tumour cell death in human and murine melanoma cells in vitro and improved survival in the murine B16 melanoma model in *** analysis pointed to a central role for p53,which was confirmed using p53^(-/-)B16 *** vivo,the additional effect of RIG-I in combination with irradiation on tumour growth was absent in mice carrying p53^(-/-)B16 tumours,while the antitumoural response to RIG-I stimulation alone was *** results identify p53 as a pivotal checkpoint that is triggered by RIG-I resulting in enhanced irradiation-induced tumour cell ***,the combined administration of RIG-I ligands and radiotherapy is a promising approach to treating radioresistant tumours with a functional p53 pathway,such as melanoma.

关键词： RIG-I p53 melanoma immunotherapy irradiation radiotherapy radioresistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：