Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity

作     者：Marianna Barbalinardo Andrea Antosova Marta Gambucci Zuzana Bednarikova Cristiano Albonetti Francesco Valle Paola Sassi Loredana Latterini(ISI) Zuzana Gazova Eva Bystrenova Marianna Barbalinardo;Andrea Antosova;Marta Gambucci;Zuzana Bednarikova;Cristiano Albonetti;Francesco Valle;Paola Sassi;Loredana Latterini;Zuzana Gazova;Eva Bystrenova

作者机构：C.N.R.-I.S.M.Nvia gobetti101bologna 40129italy Institute of experimental physicsdepartment of biophysicsslovak academy of sciencewatsonova 4704001kosiceslovakia department of chemistrybiology and biotechnologyuniversity of perugiaperugia 06123italy consorzio interuniversitario per lo sviluppo dei sistemi a grande interfase(csgi)ismn-cnrbologna 40129italy department of medical and clinical biochemistryfaculty of medicinesafarik universitykosice 04001slovakia 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2020年第13卷第4期

页      面：1081-1089页

核心收录：

学科分类：0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：This work was partially supported by Slovak grand agency VEGA 2/0145/17 APW-18-0284 Italian flagship NANOMAX N-CHEM Ministery o f Education University and Research(PRIN grant 20173L7W8K).Microscopy was carried out at the SPM@ISMN facility 

主　　题：lysozyme amyloid fibrils nanoparticles spectroscopy toxicity 

摘      要：The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related *** influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of *** physico-chemical parameters play a role in the interaction of proteins and nanoparticles in solution,thus influencing the disaggregation of preformed *** have examined the influence of two kinds of metallic nanoparticles on lysozyme amyloid fibrils using a multi-technique approach and focalized their impact on cytotoxicity on human neuroblastoma cells(SH-SY5Y).In particular,fluorescence,infrared and circular dichroism spectroscopies,optical and atomic force microscopy experiments have been carried out;the results are analyzed to rationalize the effects of these complexes on neural cell *** is remarkable,that the fibrils in the presence of AuNPs,unlike fibrils alone or with AgNPs,do not generate a significant cytotoxic effect even at high concentration and an amyloid degradation effect is visible.