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Robust transcriptional indicators of immune cell death revealed by spatiotemporal transcriptome analyses

Molecular Plant 2022年第6期15卷 1059-1075页

作者： Jose Salguero-Linares Irene Serrano Nerea Ruiz-Solani marta Salas-Gómez Ujjal Jyoti Phukan Victor Manuel González martíBernardo-Faura Marc Valls David Rengel Nuria S.Coll Centre for Research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBCampus UABBellaterra08193 BarcelonaSpain LIPM Universitéde ToulouseINRACNRS84195 Castanet-TolosanFrance INRAE GeT-PlaGeGenotoul31326 Castanet-TolosanFrance Department of Genetics Universitat de Barcelona08028 BarcelonaSpain Consejo Superior de Investigaciones Científicas(CSIC) BarcelonaSpain Department of Plant Molecular Biology and Physiology.Albrechtvon Haller Institute for Plant Sciences niversity of GottingenJulia-Lermontowa-Weg3.37077 GottingenGrmany Institut de Phammacologie et de Biologie Structurale IPBS Universite deToulouse CNRSUPSBP 64182205 route de Nartxonne310077 Toulouse Cedex 04France

Recognition of a pathogen by the plant immune system often triggers a form of regulated cell death traditionally known as the hypersensitive response(HR).This type of cell death occurs precisely at the site of pathogen recognition,and it is restricted to a few *** research has shed light on how plant immune receptors are mechanistically ***,two central key questions remain largely unresolved:how does cell death zonation take place,and what are the mechanisms that underpin this phenomenon?Consequently,bona fide transcriptional indicators of HR are lacking,which prevents deeper insight into its mechanisms before cell death becomes macroscopic and precludes early or live *** this study,to identify the transcriptional indicators of HR we used the paradigmatic Arabidopsis thaliana–Pseudomonas syringae pathosystem and performed a spatiotemporally resolved gene expression analysis that compared infected cells that will undergo HR upon pathogen recognition with bystander cells that will stay alive and activate *** data revealed unique and time-dependent differences in the repertoire of differentially expressed genes,expression profiles,and biological processes derived from tissue undergoing HR and that of its ***,we generated a pipeline based on concatenated pairwise comparisons between time,zone,and treatment that enabled us to define 13 robust transcriptional HR *** these genes,the promoter of an uncharacterized AAA-ATPase was used to obtain a fluorescent reporter transgenic line that displays a strong spatiotemporally resolved signal specifically in cells that will later undergo pathogen-triggered cell *** valuable set of genes can be used to define cells that are destined to die upon infection with HR-triggering bacteria,opening new avenues for specific and/or high-throughput techniques to study HR processes at a single-cell level.

关键词： Arabidopsis thaliana cell death indicator effector-triggered immunity hypersensitive response pattern-triggered immunity plant immunity Pseudomonas syringae

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Education in trauma： An educational alternative that promotes injury prevention

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Chinese Journal of Traumatology 2017年第5期20卷 275-277页

作者： Jose Daniel Charry Juan Daniel ochoa Jorman Harvey Tejada Sandra Liliana Navarro-Parra Nicolas Esquivel Yolercy Vasques Research Department Fundaci6n Universitaria Navarra Uninavarra Neiva Colombia Department of Medicine Universiclad Surcolombiana Neiva Colombia Research Department Universidad de Jaen Spain

Purpose： As trauma is a public health problem, different programs have been designed to prevent in- juries. The aim of this study was to evaluate the effectiveness of an educational model that measures the adolescents＇ attitudes towards the rules of road safety, alcohol and road accidents in Colombia. Methods： A pedagogical model evaluating the effect of road safety education and adolescents＇ attitudes towards and experiences of alcohol and road accidents in Colombia was created. After the education concluded, this educational process is analyzed by its impact on adolescents＇ behavior. The educational program included 160 adolescents with the mean age being 1Z5 years. Results： The test results indicated that before the educational program 80g of adolescents did not use a safety element when driving, while after the educational program the percentage of no helmet use among adolescents decreased from 72.5% to 24.3% （p = 0.0001 ） and driving a vehicle under the state of drunkenness from 49.3% to 8.1% （p = 0.0001）. Conclusion： An educational model aimed at preventing injuries caused by traffic accidents is shown to be effective in generating changes in adolescents＇ customs of and attitudes towards alcohol and road safety standards in Colombia.

关键词： Models Educational Trauma Injury prevention Alcohol

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Microstructure and hydrogen storage properties of as-cast and rapidly solidified Ti-rich Ti-V alloys

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Transactions of Nonferrous Metals Society of China 2012年第8期22卷 1831-1838页

作者： Suwarno SUWARNO Jan Ketil SOLBERG Jan Petter MAEHLEN Bente KROGH Bφrre Tore BφRRESEN Esther ochoa-FERNANDEZ Erling RYTTER Mario WILLIAMS Roman DENYS Volodymyr A.YARTYS Department of Materials Science and Engineering Norwegian University of Science and TechnologyNO-7491TrondheimNorway Institute for Energy Technology P.O.Box 40NO-2027KjellerNorway Statoil Research Centre RotvollN-7005TrondheimNorway University of the Western Cape Private Bag X17Bellville7535South Africa

The goal of the present work was to optimize the phase-structural composition and microstructure of binary Ti0.8-0.9V0.2-0.1 alloys with respect to their hydrogen sorption properties. Application of these alloys is for hydrogen absorption from gaseous mixtures containing substantial amounts of carbon monoxide （CO） at high temperatures. Irrespective of alloy composition, both α（HCP） and β（BCC） phases in Ti0.8-0.9V0.2-0.1 formed single phase FCC hydrides upon hydrogenation in pure H2. An in situ synchrotron X-ray diffraction study showed that only the β-phase transformed to the corresponding hydride when the alloy was hydrogenated in a mixture of H2＋10%CO. Rapid solidification （RS） of the alloy resulted in refined grain sizes both in the Ti0.8V0.2 and Ti0.9V0.1 alloys. Furthermore, RS was found to increase the β-phase fraction in Ti0.9V0.1, being twice larger than that of the as-cast alloy. Ti0.9V0.1 had a platelike microstructure as observed by scanning electron microscopy （SEM）, the plates were about 300 nm thick. The microstructure refinement resulted in a faster kinetics of H desorption as observed by temperature desorption spectroscopy （TDS）.

关键词： hydrogen storage Ti-V alloys rapid solidification synchrotron X-ray diffraction carbon monoxide

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深水储层非均质性描述中近海泥岩和深海泥岩的识别标准

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石油地质科技动态 2016年第2期 77-95页

作者： Jesus ochoa 宋建平张生

浊积事件的规模及其频率的确定依旧带有主观性，而且难以进行。其原因是浊积事件一般同时涉及砂和泥的沉积，而泥组分通常无法与浊流建立成因联系，因而未被纳入地层厚度研究之中。深海泥岩是主要由生物成因颗粒组成的细粒沉积岩，而近... 详细信息

浊积事件的规模及其频率的确定依旧带有主观性，而且难以进行。其原因是浊积事件一般同时涉及砂和泥的沉积，而泥组分通常无法与浊流建立成因联系，因而未被纳入地层厚度研究之中。深海泥岩是主要由生物成因颗粒组成的细粒沉积岩，而近海泥岩是由生物成因颗粒和陆源颗粒共同组成的细粒沉积岩。遗憾的是，这些基于组分的岩石定义并没有考虑沉积过程的差异。利用扫描电子显微镜、场致发射扫描电子显微镜和x射线衍射技术对取自埃尔罗萨里奥（E1Rosario）组露头（墨西哥下加利福尼亚州）和伍德福德组页岩岩心（美国俄克拉何马州）的70块样本进行了分析，揭示了两者在成岩过程方面的差别。此外，还采用192块露头样本对实验室测量结果进行了标定，并在此基础上建立了野外识别粘土组构和矿物的方法，用于戈1分浊积地层单元。深海岩中可见规则的粘土小片晶（clayplatelets）层理、极少数的絮凝体和较少量的高密度矿物。而近海岩具有以可见的絮凝体为特征的不规则的杂乱粘土组构，而且合较多的较高密度颗粒。此外，这两种岩石的粘土矿物成分也会相应地表现出一定的变化，例如深海岩中含有蒙脱石，而近海岩中含有高岭石。这些结果表明，深海岩沉积时沉积物沉降速度大于剪切速度，而近海岩则是在与之相反的沉积条件下沉积的。浊流支撑和悬浮密度较大的颗粒，形成不规则的杂乱粘土组构，并为絮凝作用提供了更多的时间。深海岩和近海岩的区分对于确定浊积事件的规模和频率具有重要的意义，而后者对多期次浊流沉积砂体的连通性和连续性有影响。此外，区分深海岩和近海岩有助于人们更好地认识泥岩储层的建筑结构。

关键词：储层非均质性泥岩识别标准深海近海扫描电子显微镜 x射线衍射技术颗粒组成

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考虑剪切和轴向效应的半刚性连接Timoshenko梁柱的大挠度和后屈曲性态

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钢结构(中英文) 2008年第1期 86-87页

作者： J.Dario Aristizabal-ochoa

研究Timoshenko梁-柱的非线性大挠度-小应变分析和后屈曲行为。这种梁具有半刚性连接的对称横截面,其连接经受保守和非保守端部荷载(力和弯矩)作用,分析中将组合剪切作用、轴向和弯曲变形、轴向偏心力、水平支撑和移位等都简化为单一的... 详细信息

研究Timoshenko梁-柱的非线性大挠度-小应变分析和后屈曲行为。这种梁具有半刚性连接的对称横截面,其连接经受保守和非保守端部荷载(力和弯矩)作用,分析中将组合剪切作用、轴向和弯曲变形、轴向偏心力、水平支撑和移位等都简化为单一的荷载作用。基于改进的剪力公式,推导出一套包括剪切变形和轴向外力导致的剪力等效应的函数集合。同时,还推出了因梁柱在端部力和弯矩作用下弯曲而产生的轴向位移δb的表达方式。尽管只是近似推算,但所提出的方法和相应的非线性方程,却可以用于带刚性、半刚性连接和铰接的Timoshenko梁-柱的拉压稳定性和非线性大挠度-小应变弹性分析。分析研究表明,剪切变形增加了纵横向的挠度,减小了梁柱由于轴向压力而产生的屈曲。在具有相对低效剪力区域或低抗剪刚度的梁柱分析中必须考虑剪切变形的作用。剪切效应在板式橡胶支座的拉压稳定性和动力特性方面的影响上同样非常重要。采用4个范例说明该方法和公式的有效性。

关键词：梁梁-柱大挠度 P-Δ分析非线性分析半刚性连接稳定性结构分析后屈曲性态 Timoshenko梁

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Fast Detection of Auxins by Microplate Technique

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American Journal of Plant Sciences 2017年第2期8卷 171-177页

作者： Julia Cecilia Anguiano-Cabello Alberto Flores-Olivas Yisa María ochoa-Fuentes Roberto Arredondo-Valdés Victor Olalde-Portugal Department of Parasitology Antonio Narro Agrarian Autonomous University Saltillo Mexico

Plant growth promotion indole-3-acetic acid (IAA) is the most abundant natural auxin that plays diverse roles in plant growth, development and plant immunity. Perturbing auxin homeostasis appears to be a common virulence mechanism, as many pathogens can synthesize auxin-like molecules. In other hand, the addition of plant growth promotion rhizobacteria (PGPR) that are able to produce auxins promotes plant growth and provides protection against pathogens. Techniques as high performance liquid chromatography (HPLC) and gas chromatography (GC) are used to quantify auxins produced by microorganism and plants at high precision and sensitivity, even though those techniques are expensive and require a big number of solvents. For these reasons, the aim of the present study was to develop a fast microplate technique for auxin detection, in Bacillus subtilis strains using salkowski reagent. For auxin quantification, calibration curves were done with alcohol, landy medium and water and the R2 were calculated. The microplate techniques were able to quantify auxin production by B. subtillis stains.

关键词： Auxin Microplate Bacillus subtilis

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新发现的激动

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医学分子生物学杂志 1982年第1期 38-41页

作者： ochoa S 杨严礼

我于1955年在伦敦国际生化协会临时委员会会议上第一次迂见马塞尔·弗洛金(Macel Florkin),他是该协会的主席并召集起草了新协会的章程,接着任1955年到1961年国际生化协会(IUB)第一任主席。1961年我继任弗洛金(Florkin)国际生化协会主... 详细信息

我于1955年在伦敦国际生化协会临时委员会会议上第一次迂见马塞尔·弗洛金(Macel Florkin),他是该协会的主席并召集起草了新协会的章程,接着任1955年到1961年国际生化协会(IUB)第一任主席。1961年我继任弗洛金(Florkin)国际生化协会主席的职务。由于他的帮助,我任此职一直到

关键词：异柠檬酸脱氢酶洛金生化 NADPH mRNA 三氯醋酸柠檬酸循环磷酸化酶糖基转移酶多聚体 ADP 核昔酸密码子实验结果丙酮酸蛋白质合成系统苹果酸酶脱氢酶氨基酸氨基羧酸次黄

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Integrated sensing and delivery of oxygen for next- generation smart wound dressings

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Microsystems & Nanoengineering 2020年第1期6卷 991-1006页

作者： Manuel ochoa Rahim Rahimi Jiawei Zhou Hongjie Jiang Chang Keun Yoon Dinesh Maddipatla Binu Baby Narakathu Vaibhav Jain Mark Michael Oscai Thaddeus Joseph Morken Rebeca Hannah Oliveira Gonzalo L.Campana Oscar W.Cummings Michael A.Zieger Rajiv Sood Massood Z.Atashbar Babak Ziaie School of Electrical and Computer Engineering Purdue UniversityWest Lafayette 47907 INUSA Birck Nanotechnololgy Center 1205W State StreetWest Lafayette 47907 INUSA School of Material Science Engineering Purdue UniversityWest Lafayette 47907 INUSA Electrical and Computer Engineering Department Western Michigan University4601 Campus Drive B-236Kalamazoo 49008 MIUSA School of Mechanical Engineering Purdue UniversityWest Lafayette 47907 INUSA Indiana University School of Medicine 980 West Walnut StreetBuilding R3 Room C634Indianapolis 46202 INUSA Department of Pathology and Laboratory Medicine Indiana University School of MedicineIU Health Pathology Laboratory350W 11th StreetRoom 4054IndianapolisIN 46202USA Weldon School of Biomedical Engineering Purdue UniversityWest Lafayette 47907 INUSA Present address:Shenzhen MSU-BIT University ShenzhenChina

Chronic wounds affect over 6.5 million Americans and are notoriously difficult to *** oxygenation of the wound bed is one of the most critical and treatable wound management factors,but existing oxygenation systems do not enable concurrent measurement and delivery of oxygen in a convenient wearable ***,we developed a low-cost alternative for continuous O 2 delivery and sensing comprising of an inexpensive,paper-based,biocompatible,flexible platform for locally generating and measuring oxygen in a wound *** platform takes advantage of recent developments in the fabrication of flexible microsystems including the incorporation of paper as a substrate and the use of a scalable manufacturing technology,inkjet ***,we demonstrate the functionality of the oxygenation patch,capable of increasing oxygen concentration in a gel substrate by 13%(5ppm)in *** platform is able to sense oxygen in a range of 5–26 *** vivo studies demonstrate the biocompatibility of the patch and its ability to double or triple the oxygen level in the wound bed to clinically relevant levels.

关键词： patch smart printing

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Interplay among cellular polarization,lipoprotein metabolism and hepatitis C virus entry

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World Journal of Gastroenterology 2011年第22期17卷 2683-2690页

作者： Ignacio Benedicto Francisca Molina-Jiménez Ricardo Moreno-Otero Manuel López-Cabrera Pedro L Majano Molecular Biology Unit Hospital Universitario de la Princesa Instituto de Investigación Sanitaria Princesa (IP) Madrid 28006 Spain CIBERehd Instituto de Salud Car- los Ⅲ Madrid 28029 Spain Liver Unit Hospital Universitario de la Princesa Instituto de Investigación Sanitaria Princesa (IP) Madrid 28006 Spain Centro de Biología Molecular Severo ochoa CSIC-UAM Madrid 28049 Spain

Hepatitis C virus (HCV) infects more than three million new individuals worldwide each year. In a high percent age of patients, acute infections become chronic, eventually progressing to fibrosis, cirrhosis, and hepatocellular carcinoma. Given the lack of effective prophylactic or therapeutic vaccines, and the limited sustained virological response rates to current therapies, new approaches are needed to prevent, control, and clear HCV *** into the host cell, being the first step of the viral cycle, is a potential target for the design of new antiviral compounds. Despite the recent discovery of the tight junction-associated proteins claudin-1 and occludin as HCV co-receptors, which is an important step towards the understanding of HCV entry, the precise mechanisms are still largely unknown. In addition, increasing evidence indicates that tools that are broadly employed to study HCV infection do not accurately reflect the real process in terms of viral particle composition and host cell phenotype. Thus, systems that more closely mimic natural infection are urgently required to elucidate the mechanisms of HCV entry, which will in turn help to design antiviral strategies against this part of the infection process.

关键词： Cellular polarization Tight junctions Lipoprotein metabolism Hepatitis C virus

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杀戮还是艺术?

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中国慈善家 2020年第4期 78-91页

作者：吴可言 Daniel ochoa de Olza(摄影) 不详

华丽的斗牛士服装、血腥的场面、奄奄一息的公牛……这项涎生于18世纪中叶的西班牙传统表演如今仍令许多观众趋之若鹜;同时它也饱受争议,以动物杀戮为乐的斗牛表演正在减少。西班牙摄影师丹尼尔·奥乔亚花了7年时间、观看上百场斗牛表... 详细信息

华丽的斗牛士服装、血腥的场面、奄奄一息的公牛……这项涎生于18世纪中叶的西班牙传统表演如今仍令许多观众趋之若鹜;同时它也饱受争议,以动物杀戮为乐的斗牛表演正在减少。西班牙摄影师丹尼尔·奥乔亚花了7年时间、观看上百场斗牛表演后拍摄出这组照片,“爱它的人为之疯狂,恨它的人嗤之以鼻。”奥乔亚告诉《中国慈善家》。

关键词：斗牛奥乔亚杀戮慈善家丹尼尔摄影师西班牙

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