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CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish(Gambusia affinis Baird and Girard 1853)juveniles

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Emerging Contaminants 2022年第1期8卷 280-287页

作者： Moh Awaludin Adam Agoes Soegianto Carolyn Melissa Payus Ach Khumaidi Ramli Ramli ernawati ernawati Irawati Mei Widiastuti Era Insivitawati Yenny Risjani Faculty of Science and Technology University of IbrahimySitubondoIndonesia Research Center for Marine and Land Bioindustry National Research and Innovation AgencyLombokNTBIndonesia Department of Biology Faculty of Science and TechnologyUniversitas AirlanggaSurabayaIndonesia Faculty of Science and Natural Resources Universiti Malaysia SabahKota KinabaluSabahMalaysia Aquatic Product Technology Department Faculty of AgricultureUniversity of YudhartaPasuruanEast JavaIndonesia Aquaculture Department Faculty of Animal Husbandry and FisheriesUniversity of TadulakoPaluCentral SulawesiIndonesia Politeknik Kementerian Kelautan dan Perikanan SidoarjoJawa TimurIndonesia Department of Aquatic Resources Management Faculty of Fisheries and Marine SciencesBrawijaya UniversityMalangIndonesia

This experiment aimed to determine how Cd exposure impacts CD4 cell activation,macrophage cells,pinocytosis activity,metallothionine expression,and Cd levels in juvenile Gambusia affinis *** treatment groups were used,with one control and treatments A,B,C,and D at dosages of 0.03 mg/L,0.023 mg/L,0.015 mg/L,and 0.008 mg/L,*** results showed that the number of CD4 with CD8 cell markers differed significantly from each treatment compared to the control(0.33%).The value in A,namely 0.54%,was the highest,followed by B,C,and D of 0.46%,0.44%,and 0.42%,*** number of macrophages increased as the Cd level of the medium increased,as did the activity of ***,the immunofluorescence test on the gills with the Anti-MT Mouse and goat IgG fluoresce in Rhodamine on the gills showed that luminescence increased with increasing Cd levels in the ***,the MT intensity increased at Cd-exposed gills as compared to the *** the Cd level in the gills increased,the MT levels ascended significantly.

关键词： Cadmium CD4 cell Fish health Immunity Metallothionein Water pollution

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Diversity and abundance of mangrove fiddle crabs, genus Uca(Decapoda, Ocypodidae) at a mangrove in Kema,North Sulawesi, Indonesia

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Acta Oceanologica Sinica 2018年第12期37卷 92-96页

作者： RIANTA Pratiwi ernawati Widyastuti CHEN Guangcheng CHEN Shunyang Research Center for Oceanography Indonesian Institute of Sciences Third Institute of Oceanography Ministry of Natural Resources

Mangrove ecosystems are sites with high biodiversity of benthic fauna, and fiddler crabs(genus Uca) are common benthic fauna in mangroves. The North Sulawesi in Indonesia has a good condition of mangrove while the information of the fiddler crabs is still limited. Manual samplings were conducted in wet, dry and transient seasons at a mangrove in Kema, North Sulawesi to investigate the species composition, density and distribution pattern of fiddler crabs. A total of 168 individuals, subjected to eight species of genus Uca crabs were collected at the mangrove, with U. triangularis having the highest abundance and U. annulipes having the lowest *** densities of fiddle crabs were 27.56 ind./m2, 32.89 ind./m2and 14.22 ind./m2at the seaward, middle and landward zones, respectively, and the density was higher in dry and wet seasons than in transient season.

关键词： Crustacea genus Uca species mangrove North Sulawesi Indonesia

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Bioinspired cellulose-based membranes in oily wastewater treatment

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Frontiers of Environmental Science & Engineering 2022年第7期16卷 139-172页

作者： Abdul Halim Lusi ernawati Maya Ismayati Fahimah Martak Toshiharu Enomae Department of Chemical Engineering Universitas Internasional Semen IndonesiaGresik61122Indonesia Department of Chemical Engineering Kalimantan Institute of TechnologyBalikpapan76127Indonesia Research Center for Biomaterials National Research and Innovation Agency(BRIN)Bogor16911Indonesia Department of Chemistry Faculty of SciencesSepuluh Nopember Institute of TechnologyKampus ITS SukoliloSurabaya60111Indonesia Faculty of Life and Environmental Sciences University of TsukubaTsukuba305-8572Japan

It is challenging to purify oily wastewater, which affects water-energy-food production. One promising method is membrane-based separation. This paper reviews the current research trend of applying cellulose as a membrane material that mimics one of three typical biostructures: superhydrophobic, underwater superoleophobic, and Janus surfaces. Nature has provided efficient and effective structures through the evolutionary process. This has inspired many researchers to create technologies that mimic nature’s structures or the fabrication process. Lotus leaves, fish scales, and Namib beetles are three representative structures with distinct functional and surface properties: superhydrophobic, underwater superoleophobic, and Janus surfaces. The characteristics of these structures have been widely studied and applied to membrane materials to improve their performance. One attractive membrane material is cellulose, whichhas been studied from the perspective of its biodegradability and sustainability. In this review, the principles, mechanisms, fabrication processes, and membrane performances are summarized and compared. The theory of wettability is also described to build a comprehensive understanding of the concept. Finally, future outlook is discussed to challenge the gap between laboratory and industrial applications.

关键词： Cellulose Bioinspired membrane Superhydrophobic surface Underwater superoleophobic surface Oil-water separation

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Hepatoprotective and antioxidant activity of pentagamavunon-0 against carbon tetrachloride-induced hepatic injury in rats

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Asian Pacific Journal of Tropical Medicine 2013年第6期6卷 438-442页

作者： Arief Nurrochmad Supardjan Amir Margono Sardjiman Arief Rahman Hakim ernawati Erna Kurniawati Erva Fatmawati Department of Pharmacology and Clinical Pharmacy Faculty of PharmacyGadjah Mada UniversitySekip Utara Yogyakarta-55281Indonesia Curcumin Research Center Faculty of PharmacyCadjah Mada UniversitySekip Utara Yogyakarta-55281Indonesia

Objective:To investigate the hepatoproteetivc ami antioxidant activity of pentagamavunon-0(PGV-0) against CCl-4-induced hepatic injury in ***:The groups of animals were administered with PGV-0 at die doses 2.5.5,10,and 20 mg/kg b.w.,*** in a day for 6 days and at day 7 the animals were administrated with carbon tetrachloride(CClj)(20%,2 ml/kg *** liquid paraffin dp.).The effect of PGV-0 on serum transaminase(SGPT),alkaline phosphates(ALP and total bilirubin were determined in CCl-4-indueed hepatotoxicity in ***,the effects of PGV-0 on glutathione(GSU) content,cutalase(CAT) and NO free radical scavenging activity also were ***:The results demonstrated that PCV-0 significantly reduced the activity of SGPT,serum ALP and total bilirubin in CCl-4 induced rat ***-0 has effect on the antioxidant and free radical defense *** prevented the depletion level of GSH and decrease activity of CAT in CCl-4-induced liver injury in ***-0 also demonstrated the free radical scavenger effects on NO free radical scavenging activity with ES value of 32.32μM. Convulsion:All of our findings suggests that PGV-0 could protect the liver cells from CCl-4- induced liver damages and the mechanism may through the antioxidative effect of PGV-0 to prevent the accumulation of free radicals and protect the liver damage.

关键词： Hepatoprotective Antioxidant Pentagamavunon-0 Carbon tetrachloride Rats

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Carbon Sources of Sediment and Epifaunal Food Sources in a Tropical Mangrove Forest in North Sulawesi, Indonesia

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Journal of Ocean University of China 2020年第5期19卷 1169-1176页

作者： WU Zhiqiang XIE Limei CHEN Bin DHAMAWAN I Wayan Eka SASTROSUWONDO Pramudji CHEN Shunyang RIANTA Pratiwi ernawati Widyastuti CHEN Guangcheng College of Marine Life Sciences Ocean University of ChinaQingdao 266000China Third Institute of Oceanography Ministry of Natural ResourcesXiamen 361005China Research Center for Oceanography Indonesian Institute of SciencesJakarta 11048Indonesia Observation and Research Station of Coastal Wetland Ecosystem in Beibu Bay Ministry of Natural ResourcesBeihai 536015China

The stable isotope ratios of nitrogen(δ^(15)N) and carbon(δ^(13)C) were applied in this study to analyze sediment carbon sources and primary food sources of epifauna in an oceanic mangrove forest in tropical North Sulawesi, Indonesia. Proportional contribution of mangrove-derived carbon to the food sources was compared among epifauna belonging to different feeding guilds. The sediment in the mangrove site with a depleted δ^(13)C signature(-28.02‰± 0.24‰) mainly consists of mangrove-derived carbon. Analysis using Bayesian stable isotope mixing model in the R program shows that mangrove-derived carbon can dominate the food sources of a majority of the epifauna(11 out of the total 18 species), especially 2 sesarmid crabs Parasesarma semperi and Sesarma sp., crab Epixanthus dentatus, and snails Terebralia sulcata and Optediceros breviculum, which belong to various feeding guilds. Mangrove-derived carbon has a proportional contribution to the food sources of fiddle crab Tubuca coarctata and 3 littorinid snails(Littoraria spp.) close to that of suspended particulate organic matter. Three planktophagous bivalve species(Anadara antiquata, Anadara sp. and Callista erycina) were found to mainly feed on seagrass-derived materials. Mean proportional contributions of mangrove-derived carbon to the food sources were 50.15%, 59.60%, 46.20% and 27.58% for the carnivorous, omnivorous, phytophagous and planktophagous groups, respectively. The results suggest that mangrove-derived carbon can directly(via grazing plant tissues) or indirectly(via deposit feedings) make an important contribution to the food sources of epifauna in the oceanic mangrove forest where the allochthonous input of organic carbon is low.

关键词： epifauna stable isotope carbon nitrogen sediment feeding guilds

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Distribution of Axial Length, Anterior Chamber Depth and Lens Thickness of Pre-Operative Cataract Patients in Indonesian Population

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Open Journal of Ophthalmology 2020年第4期10卷 288-296页

作者： Titiek ernawati Maharani Kartika Anggraeni Kevin Anggakusuma Hendrawan Rianti Wulandari Pratiwi Ophthalmology Department Faculty of Medicine Widya Mandala Catholic University Surabaya Surabaya Indonesia Ophthalmology Department Primasatya Husada Citra Hospital Surabaya Indonesia Faculty of Medicine Airlangga University Surabaya Indonesia

Purpose: Biometry measurement of pre-operative cataract patients plays a major role in calculating intraocular lens power. This study aimed to review and determine the distribution of biometry components, such as axial length (AL), anterior chamber depth (ACD), and lens thickness (LT) of pre-operative cataract patient in Indonesian population. Methods: A cross-sectional study data were obtained from 2015-2018 cataract surgery at Prima Husada Citra Hospital, Surabaya, East Java, Indonesia. A total of 1295 eyes with cataract was included in the study based on their medical record. We analyzed the data using descriptive analysis and correlated each variable using the Spearman’s Rho analysis. Results: Mean AL, LT and ACD was 23.81 ± 1.46 mm, 4.49 ± 0.55 mm, 3.25 ± 0.70 mm respectively. Male has longer AL and deeper ACD with (p p = 0.005). The increase of AL was accompanied by ACD (r = 0.457;p r = -0.101;p Conclusions: LT is increased with age while AL and ACD are shortened with age. There was a positive correlation between AL and ACD, but an inverse correlation between AL and LT.

关键词： Anterior Chamber Depth Axial Length Cataract Lens Thickness

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Hypoxic Preconditioning Improved Neuroprotective Effect of Bone Marrow-Mesenchymal Stem Cells Transplantation in Acute Glaucoma Models

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Journal of Biomedical Science and Engineering 2016年第4期9卷 245-257页

作者： Titiek ernawati Gatut Suhendro I Ketut Sudiana Suhartono Taat Putra Harjanto JM Sunarjo Agus Turchan Fedik Abdul Rantam Department of Ophthalmology School of Medicine Widya Mandala University Surabaya Indonesia PHC Hospital Surabaya Indonesia Department of Ophthalmology School of Medicine Airlangga University Surabaya Indonesia Dr. Soetomo General Hospital Surabaya Indonesia Department of Pathology Anatomy School of Medicine Airlangga University Surabaya Indonesia Department of Physiology School of Medicine Airlangga University Surabaya Indonesia Department of Public Health School of Medicine Airlangga University Surabaya Indonesia Department of NeuroSurgery School of Medicine Airlangga University Surabaya Indonesia Laboratory of Stem Cell Institute of Tropical Disease Airlangga University Surabaya Indonesia Regenerative Medicine & Stem Cell Centre Airlangga University Surabaya Indonesia Laboratory of Virology and Immunology Department of Microbiology School of Veterinary Medicine Airlangga University Surabaya Indonesia

This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells (RGCs) in Acute Glaucoma Models. The methods of this research were isolated mesenchymal stem cells from the bone marrow of adult wild-type Sprague-Dawley (SD) rats. BM-MSCs were cultured under normoxic or hypoxic (1% oxygen for 24 hours) conditions. Normoxic or hypoxic BM-MSCs were transplanted intravitreally 1 week after ocular hypertension induction by acutely increasing IOP to 100 - 120 mmHg for 60 minutes. Rats were killed 4 weeks after transplanted. Apoptosis was examined by tunnel assay and expression Brn3b (Brn3b = RGCs marker) by immunohistochemical analysis of the retina. Results showed that transplantation of hypoxic preconditioning BM-MSCs in acute glaucoma models resulted in a significant apoptosis decreasing (p < 0.05) and an significant increasing in RGCs (p < 0.05), as well as enhanced mor-phologic and functional benefits of stem cell therapy versus normoxic BM-MSCs transplantation. Conclusions: Hypoxic preconditioning enhances the capacity of BM-MSCs transplantation to improve neuroprotective effects of RGCs in Acute Glaucoma Models.

关键词： Hypoxic Preconditioning Transplantation Bone Marrow-Mesenchymal Stem Cells BM-MSCs Glaucoma Neuroprotective

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马来西亚鸡新城疫的免疫:油剂乳化疫苗的应用

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青海畜牧兽医杂志 1985年第3期 30-30页

作者： ernawati R. 景鲁

一种油剂乳化鸡新城疫疫苗,对童鸡免疫试验的效果评价表明。一组一日龄雏鸡用缓发性鸡新城疫消毒疫苗免疫,随后在3与8周龄时用油剂乳化疫苗免疫后,得到了满意的结果,血凝抑制抗体应答维持至18周。免疫鸡用速发型亲内脏的鸡新城疫强毒攻... 详细信息

一种油剂乳化鸡新城疫疫苗,对童鸡免疫试验的效果评价表明。一组一日龄雏鸡用缓发性鸡新城疫消毒疫苗免疫,随后在3与8周龄时用油剂乳化疫苗免疫后,得到了满意的结果,血凝抑制抗体应答维持至18周。免疫鸡用速发型亲内脏的鸡新城疫强毒攻击时,有90—100％的保护率。尽管免疫能保护有症状的鸡,仍能分出残留病毒,而攻毒后10—20天后所有免疫鸡再无鸡新城疫的感染。

关键词：鸡新城疫疫苗免疫油剂农药剂型

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