Hypoxic Preconditioning Improved Neuroprotective Effect of Bone Marrow-Mesenchymal Stem Cells Transplantation in Acute Glaucoma Models

作     者：Titiek Ernawati Gatut Suhendro I Ketut Sudiana Suhartono Taat Putra Harjanto JM Sunarjo Agus Turchan Fedik Abdul Rantam Titiek Ernawati;Gatut Suhendro;I Ketut Sudiana;Suhartono Taat Putra;Harjanto JM;Sunarjo;Agus Turchan;Fedik Abdul Rantam

作者机构：Department of Ophthalmology School of Medicine Widya Mandala University Surabaya Indonesia PHC Hospital Surabaya Indonesia Department of Ophthalmology School of Medicine Airlangga University Surabaya Indonesia Dr. Soetomo General Hospital Surabaya Indonesia Department of Pathology Anatomy School of Medicine Airlangga University Surabaya Indonesia Department of Physiology School of Medicine Airlangga University Surabaya Indonesia Department of Public Health School of Medicine Airlangga University Surabaya Indonesia Department of NeuroSurgery School of Medicine Airlangga University Surabaya Indonesia Laboratory of Stem Cell Institute of Tropical Disease Airlangga University Surabaya Indonesia Regenerative Medicine & Stem Cell Centre Airlangga University Surabaya Indonesia Laboratory of Virology and Immunology Department of Microbiology School of Veterinary Medicine Airlangga University Surabaya Indonesia 

出 版 物：《Journal of Biomedical Science and Engineering》 (生物医学工程（英文）)

年 卷 期：2016年第9卷第4期

页      面：245-257页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

主　　题：Hypoxic Preconditioning Transplantation Bone Marrow-Mesenchymal Stem Cells BM-MSCs Glaucoma Neuroprotective 

摘      要：This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells (RGCs) in Acute Glaucoma Models. The methods of this research were isolated mesenchymal stem cells from the bone marrow of adult wild-type Sprague-Dawley (SD) rats. BM-MSCs were cultured under normoxic or hypoxic (1% oxygen for 24 hours) conditions. Normoxic or hypoxic BM-MSCs were transplanted intravitreally 1 week after ocular hypertension induction by acutely increasing IOP to 100 - 120 mmHg for 60 minutes. Rats were killed 4 weeks after transplanted. Apoptosis was examined by tunnel assay and expression Brn3b (Brn3b = RGCs marker) by immunohistochemical analysis of the retina. Results showed that transplantation of hypoxic preconditioning BM-MSCs in acute glaucoma models resulted in a significant apoptosis decreasing (p 0.05) and an significant increasing in RGCs (p 0.05), as well as enhanced mor-phologic and functional benefits of stem cell therapy versus normoxic BM-MSCs transplantation. Conclusions: Hypoxic preconditioning enhances the capacity of BM-MSCs transplantation to improve neuroprotective effects of RGCs in Acute Glaucoma Models.