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Immune response to inactivated bacterial vector carrying the recombinant K39 antigen of Leishmania infantum in mice

Asian pacific Journal of tropical Biomedicine 2024年第5期14卷 199-206页

作者： Lucelina S.Araújo Bruno B.Silva Eduarda N.F.N.Santos Arnaldo S.Bezerra Samuel S.Frota Assis R.Montenegro eridan o.p.t.florean Maurício Fvan tilburg Maria Izabel F.Guedes Laboratory of Biotechnology and Molecular Biology Northeast Biotechnology NetworkState University of CearáFortalezaBrazil Faculty of Veterinary Medicine State University of CearáFortalezaBrazil

objective:to evaluate the immunological response elicited by an inactivated bacterial vector carrying the K39 antigen of Leishmania infantum,and a purified ***:Mice were subjected to the following treatments:(1)purified recombinant K39(rK39)protein at a 20μg dose with complete Freund’s adjuvant;(2)Inactivated Escherichia coli(BL21 DE3)carrying the K39 protein at an equivalent total protein content of 200μg;(3)Inactivated bacteria lacking the K39 protein;(4)Non-immunized control *** monitoring was *** groups were challenged by intraperitoneal injection of 10^(7) Leishmania infantum *** euthanasia,the liver and spleen were collected to analyze the levels of tNF,IFN-γ,IL-12,IL-4,and ***:Mice immunized with purified rK39 or the inactivated bacterial vector carrying the K39 antigen of Leishmania infantum showed a long-lasting immune response with high levels of polyclonal antibodies specifically recognizing the recombinant *** IgG1 subclass was the predominant immunoglobulin;however,the induction of IgG2a and the profile of cytokines produced were indicative of the induction of a mixed-type ***:the inactivated bacterial vector carrying the K39 antigen,as well as the purified antigen can induce a long-lasting immune response in immunized mice,predominantly favouring a th2 profile response.

关键词： Visceral leishmaniasis K39 Inactivated bacterial vector Vaccine Immune response th1 th2 Leishmania infantum

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Antidiabetic effects of galactomannans from Adenanthera pavonina *** streptozotocin-induced diabetic mice

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Asian pacific Journal of tropical Medicine 2018年第2期11卷 116-122页

作者： Icaro Gusmao pinto Vieira Francisca Noelia pereira Mendes Sabrina Cesar da Silva Raquel teixeira terceiro paim Bruno Bezerra da Silva Stephen Rathinaraj Benjamin eridan orlando pereira tramontina florean Maria Izabel Florindo Guedes technological Development park(pADEtEC) Federal University of CearaPici Campus60455-970FortalezaCearaBrazil Department of Health and Nutrition State University of CearaAv.Dr.Silas Munguba 1700Itaper campus60714-903ForalezaCearaBrazil

objective: to evaluate the antidiabetic effect of galactomannans extracted from Adenanthera pavonina's ***(GAp) in streptozotocin(StZ) induced diabetic ***:the preliminary galactomannan yield from Adenanthera pavonina *** and extraction products composition were *** chemical characterization methods like thin layer chromatography, Fourier transform infrared spectroscopy, ~1H and ^(13)C nuclear magnetic resonance, and molecular weight by gel permeation chromatography have been employed to characterize the extracted *** mice were divided in four groups: Normal control, diabetic control, GAp(1% and 2%) treated and standard drug treated *** mice received treatment daily for 30 *** was induced by StZ at a single dose of 120 mg/*** weight, water and food intake, fasting blood glucose, total cholesterol and triglycerides were *** analysis of pancreas and liver were performed to evaluate StZ-induced tissue ***: the isolated and extracted galactomannan from Adenanthera pavonina was confirmed by various chemical characterization *** exhibited a 1.46:1 mannose: galactose ratio, and high molar *** GAp enriched food decreased glycaemia, total cholesterol and *** didn't interfere on food intakes or body weight, although it increased water ***, the relative liver weight indicated toxic galactomannan effects on the histopathological changes of the pancreas in StZ induced ***: It is concluded that GAp is a natural product that contains potent galactomannan and is useful in preventing and treating diabetes.

关键词： Adenanthera pavonina Galactomannans Diabetes Glycaemia total cholesterol triacylglycerol

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Characterization of Cnidoscolus quercifolius pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines

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Asian pacific Journal of tropical Biomedicine 2018年第7期8卷 345-351页

作者： paulo Fernando Machado paredes Selene Maia de Morais Fernando Cesar Rodrigues Brito Luiz Francisco Wemmenson Goncalves Moura patricia de Araujo Rodrigues Stephen Rathinaraj Benjamin Francisco Ernani Alves Magalhaes eridan orlando pereira tramontina florean Maria Izabel Florindo Guedes Northeast Biotechnology Network Graduate Program of Biotechnology State University of Ceara Itaperi campus Department of Chemistry State University of Ceara Itaperi campus Laboratory of Bioprospecting of Natural products and Biotechnology State University of CearaCECITEC

objective: to evaluate the chemical components of active extract from Cnidoscolus quercifolius root bark and its cytotoxic potential against several tumor strains. Methods: the high-performance liquid chromatography with diode-array detection and ~1H and ^(13)C nuclear magnetic resonance spectroscopy of the extract were used to distinguish the existence of possible functional groups in the root bark extract. the in vitro cytotoxic activity of methanol extract on human colon cancer cell lines was evaluated using oVCAR-8, SF-295, HCt-116, HL-60 strains and the samples were assessed by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide method. Results: the analysis of nuclear magnetic spectra of the active chloroform fraction revealed the presence of absorptions bands correspondent to a mixture of favelines such as neofavelanone, deoxofaveline or methyl-faveline, which structures were confirmed by ultraviolet spectra upon high-performance liquid chromatography with diode-array detection analysis. the active fraction showed cytotoxic effects in the tested strains, HCt-116, SF-295, oVCAR-8 and HL-60 cells with IC_(50) of 72 hours ranging from 4.95 to 15.23 μg/mL. Conclusions: the results suggest that the substances present in faveleira(Cnidoscolus quercifolius) root bark extract have a cytotoxic potential against several tumor lines, showing a broader antitumour potential, and in addition no adverse to healthy cells. therefore, the root bark extract of Cnidoscolus quercifolius has a possibility of use for anticarcinogenic therapies.

关键词： Cnidoscolus quercifolius pohl. methyl-faveline Deoxofaveline Neofavelanone Faveline Cytotoxicity

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