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Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis(Diptera:Tephritidae)

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Insect Science 2021年第5期28卷 1326-1337页

作者： Qiu-Li Hou er-Hu Chen Wei Dou Jin-jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqing 400715China College of Horticulture and Plant Protection Yangzhou UniversityYangzhouJiangsu 225009China

Cuticular proteins(CPs)are critical components of the insect cuticle and play important roles in maintaining normal insect development and defense against various environmental *** oriental fruit fly(Bactrocera dorsalis)is one of the most destructive pests worldwide,and its eight CPs analogous to peritrophin 3(BdCPAP3)family genes have been identified in our prev ious *** the present study,we further explored the possible roles of CPAP3 genes in *** *** sequence of BdCPAP3 genes contained three conserved ChtBD2(chitin-binding)*** and temporal expression patterns revealed that the four BdCPAP3 genes(BdCPAP3-AI,B,E,and E2)might play important roles in larval pupariation of ***,treatment with a juvenile hormone analog(methoprene)significantly restricted expression of these four CPAP3 genes,whereas treatment with 20-hydroxy-ecdysone induced *** RNA interference(RNAi)results revealed that down-regulated CPAP3 genes led to signif-icant delay of pupariation,and injection of dsBdCPAP3-E into 5-d-old *** larvae caused approximately 40%***,we also confirmed that BdCPAP3-D2 was involved in *** ovarian *** study showed that some specific CP4P3 genes had crucial roles in *** development,and these CP genes could be used as potential targets to control this pest via RNAi.

关键词： Bactrocera dorsalis CPAP3 family expression patterns larval-pupal tran-sition ovarian development

Effects of salinity on activities of H^+-ATPase, H^+-PPase and membrane lipid composition in plasma membrane and tonoplast vesicles isolated from soybean(Glycine max L.) seedlings

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Journal of Environmental Sciences 2005年第2期17卷 259-262页

作者： YUBing-jun LAMHon-ming SHAOGui-hua LIUYou-ling CollegeofLifeSciences NanjingAgriculturalUniversityNanjing210095China／／DepartmentofBiologyTheChineseUniversityofHongKongShatinN.T.HongKongChina DepartmentofBiology TheChineseUniversityofHongKongShatinN.T.HongKongChina DepartmentofBiology TheChineseUniversityofHongKongShatinN.T.HongKongChina／／InstituteofCropBreedingandCultivationChineseAcademyofAgriculturalSciencesBeijing100081China CollegeofLifeSciences NanjingAgriculturalUniversityNanjing210095China

The effects of NaCl stress on the H +-ATPase, H +-PPase activity and lipid composition of plasma membrane(PM) and tonoplast(TP) vesicles isolated from roots and leaves of two soybean cultivars(Glycine max L.) differing in salt tolerance(Wenfeng7, salt-tolerant; Union, salt-sensitive) were investigated. When Wenfeng7 was treated with 0.3%(W/V) NaCl for 3 d, the H +-ATPase activities in PM and TP from roots and leaves exhibited a reduction and an enhancement, respectively. The H +-PPase activity in TP from roots also increased. Similar effects were not observed in roots of Union. In addition, the increases of phospholipid content and ratios of phospholipid to galactolipid in PM and TP from roots and leaves of Wenfeng7 may also change membrane permeability and hence affect salt tolerance.

关键词： salinity Glycine max L. plasma membrane tonoplast H +-ATPase H +-PPase membrane lipid composition

A Novel Multiscale Reinforcement by In-Situ Growing Carbon Nanotubes on Graphene Oxide Grafted Carbon Fibers and Its Reinforced Carbon/Carbon Composites with Improved Tensile Properties

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Journal of Materials Science & Technology 2016年第5期32卷 419-424页

作者： Yunyu Li ling-jun Guo Ya-wen Wang He-jun Li Qiang Song State Key Laboratory of Solidification Processing Northwestern Polytechnical University Center for Nano Energy Materials Northwestern Polytechnical University

In-situ growing carbon nanotubes （CNTs） directly on carbon fibers （CFs） always lead to a degraded tensile strength of CFs and then a poor fiber-dominated mechanical property of carbon/carbon composites （C/ Cs）. To solve this issue, here, a novel carbon fiber-based multiscale reinforcement is reported. To synthesize it, carbon fibers （CFs） have been first grafted by graphene oxide （GO）, and then carbon nanotubes （CNTs） have been in-situ grown on GO-grafted CFs by catalytic chemical vapor deposition. Characterizations on this novel reinforcement show that GO grafting cannot only nondestructively improve the surface chemical activity of CFs but also protect CFs against the high-temperature corrosion of metal catalyst during CNT growth, which maintains their tensile properties. Tensile property tests for unidirectional C/Cs with different preforms show that this novel reinforcement can endow C/C with improved tensile properties, 32% and 87% higher than that of pure C/C and C/C only doped with in-situ grown CNTs. This work would open up a possibility to fabricate multiscale C/Cs with excellent global performance.

关键词： Multiscale reinforcement Carbon fiber Graphene oxide Carbon nanotube Carbon/carbon composite Tensile property

Microstructure and properties of hydrophobic films derived from Fe-W amorphous alloy

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International Journal of Minerals,Metallurgy and Materials 2014年第4期21卷 395-400页

作者： Song Wang Yun-han ling jun Zhang Jian-jun Wang Gui-ying Xu School of Materials Science and Engineering University of Science and Technology Beijing School of Materials Science and Engineering Tsinghua University

Amorphous metals are totally different from crystalline metals in regard to atom arrangement. Amorphous metals do not have grain boundaries and weak spots that crystalline materials contain, making them more resistant to wear and corrosion. In this study, amorphous Fe-W alloy films were first prepared by an electroplating method and were then made hydrophobic by modification with a water repellent （heptadecafluoro-1,1,2,2-tetradecyl） trimethoxysilane. Hierarchical micro-nano structures can be obtained by slightly oxidizing the as-deposited alloy, accompanied by phase transformation from amorphous to crystalline during heat treatment. The mi-cro-nano structures can trap air to form an extremely thin cushion of air between the water and the film, which is critical to producing hydrophobicity in the film. Results show that the average values of capacitance, roughness factor, and impedance for specific surface areas of a 600°C heat-treated sample are greater than those of a sample treated at 500°C. Importantly, the coating can be fabricated on various metal substrates to act as a corrosion retardant.

关键词： iron tungsten alloys amorphous films hydrophobicity microstructure contact angle capacitance

Spatial distribution of aerosol pollution based on MODIS data over Beijing,China

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Journal of Environmental Sciences 2007年第8期19卷 955-960页

作者： LI ling-jun WANG Ying ZHANG Qiang YU Tong ZHAO Yue JIN jun Beijing Municipal Environmental Monitoring Center Beijing 100044 China. Central University for Nationalities Beijing 100081 China Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China

With the help of regression analysis, the relationships were detected between aerosol＇s contribution to apparent reflectance （ACR） derived from Moderate Resolution Imaging Spectroradiometer （MODIS） on board Terra and hourly PM10 mass concentration measured at 30 ground-based locations in Beijing for the August of 2003 and 2004. It was shown that there was a good correlation between the ACR and PM10 （linear correlation coefficient, R=0.56）. On the basis of this relationship, spatial distribution and possible sources of PM10 derived from MODIS were analyzed and two frequently heavily-polluted regions were found, namely downtown of the city and the district near Xishan Mountain. These two regions coincidently are also urban heat island centers. The foundings of this paper will be greatly useful for environmental monitoring and urban planning for Beijing, especially for the 2008 Olympic game to be held in Beijing.

关键词： MODIS PM10 spatial distribution

THerMO-RESPONSIVE BLOCK COPOLYMerS BASED ON LINEAR-TYPE POLY(ETHYLENE GLYCOL):TUNABLE LCST WITHIN THE PHYSIOLOGICAL RANGE

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Chinese Journal of Polymer Science 2012年第5期30卷 770-776页

作者： Guo-liang Yue Qian-ling Cui Yu-xi Zhang er-jian Wang 吴飞鹏 Technical Institute of Physics and Chemistry Chinese Academy of SciencesBeijing100190China Graduate School of Chinese Academy of Sciences Beijing100049China

Therrno-responsive block copolymers poly（ethylene glycol）-block-poly（N-acryloyl-2,2-dimethyl-1,3-oxazolidine）, PEG-b-PADMO, based on linear PEG were prepared via a versatile reversible addition-fragmentation chain transfer （RAFT） polymerization, PEG22 （Mw = 1000） was used as the hydrophilic component, whose dehydration was the main driving force for the phase transition of these copolymers, as demonstrated by the tH-NMR spectra. Their lower critical solution temperatures （LCSTs） could be tuned in the range of 20℃ to 35℃, by adjusting the degree of polymerization （DP） of PADMO between 14-27. Furthermore, a sharp phase transition at ca. 33℃, close to the physiological temperature with minimal hysteresis, was observed for the PEGzz-b-PADMO14 copolymer. Moreover, excellent reversibility and reproducibility were displayed for the same copolymer over 10 cycles of repeated temperature change between 25℃ （below the LCST） and 40℃ （above the LCST）.

关键词： Thermo-responsive Block copolymers Linear PEG Phase transitions.

Reaction Mechanism of H2-Assisted C3H6-SCR over Ag-CexZr Catalyst as Investigated by In situ FTIR

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Chemical Research in Chinese Universities 2020年第5期36卷 885-893页

作者： DUAN jun ZHAO ling GAO Shengjun ZHANG Yu School of Ecology and Environment Inner Mongolia UniversityHohhot 010021P.R.China

A series of silver-doped cerium zirconium oxide(Ag-CevZr)samples was synthesized successfully for selective catalytic reduction of nitric oxide(NO)with hydrogen and propene(H2/C3H6-SCR)under excess oxygen *** catalytic activity test proved that Ag-Ce0.4Zr exhibited the best C3H6-SCR ***(H2)significantly enlianced NO conversion and widened the temperature ***-technology characterizations were conducted to ascertain the properties of fabricated catalysts including X-ray diflraction(XRD),Fourier transform infrared spectrometry(FTIR),scanning electron microscopy(SEM)and H2 temperature programmed reduction(H2-TPR).In situ FTIR results demonstrated that various types of nitrates and chelating nitrite were generated on Ag-CexZr after introduction of ***,adding H2 could increase the concentration of bidentate nitrate and chelated bidentate nitrate dramatically,especially for Ce0.4Zr *** reaction between pre-adsorbing NO and C3H/C3H6+H2 illuminated that the most active intermediate was chelating nitrite,which was promoted significantly by H2 ***,adding H2 improved the formation of organo-nitro(R-N02),which was the key intennediate in *** reaction mechanism over Ag-CexZr catalysts was proposed at 200℃ as follows:nitric oxide(NO)+pr opene(C 3H6)+hy dr ogen(H2)+oxy gen(O2)→chelating nitrite (N O2-)+acry late-type species(CxHyOz)→organo-nitro(R-NO2)→isocyanate(-NCO)+cyanide(—CN)→nitrogen(N2).

关键词： NO3-H2/CH-SCR In situ Fourier transform infrared(FTIR)spectrometry

Characterization of nanoparticle mixed 316L powder for additive manufacturing

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Journal of Materials Science & Technology 2020年第12期47卷 162-168页

作者： Wengang Zhai Wei Zhou Sharon Mui ling Nai jun Wei School of Mechanical and Aerospace Engineering Nanyang Technological University50 Nanyang Avenue639798Singapore Singapore Institute of Manufacturing Technology 73 Nanyang Drive637662Singapore

Nanoparticles reinforced steels have many advantaged mechanical *** manufacturing offers a new method for fabricating nanoparticles reinforced high performance metal *** this work,we report the application of low energy ball milling in mixing nanoparticles and micron 316 L *** this method,0.3 and 1.0 wt% Y2 O3 nanoparticles can be uniformly distributed on the surface of 316 L powder with the parameters of ball-to-powder ratio at 1:1,speed at 90 rpm and 7 h of *** matrix 316 L powders remain spherical in shape after the mixing *** the meantime,the effect of low energy ball milling and the addition of Y2 O3 nanoparticles on the powder characteristics(flowability,apparent density and tap density) are also *** show that the process of low energy ball milling itself can slightly decrease the flowability and apparent density of the 316 L *** addition of 0.3 and 1.0 wt% Y2 O3 nanoparticles can also decrease the flowability,the tap density and the apparent density compared with the original 316 L *** of these changes result from the rough surface of the mixed powder produced by ball milling and the addition of Y2 O3 *** powder’s rough surface can increase the coefficient of friction of *** mixture of 316 L powder and Y2 O3 nanoparticles can be successfully used for selective laser melting(SLM).The relative density of SLM 316 L-Y2 O3 is measured at 99.5%.However,Y2 O3 agglomerations were observed which is due to the poor wettability between 316 L and Y2 O3.

关键词： Powder mixing Powder characterization Flowability Apparent density Tap density Additive manufacturing

Sequential occurrence of T790M mutation and small cell lung cancer transformation in EGFR-positive lung adenocarcinoma:A case report

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World Journal of Clinical Cases 2022年第9期10卷 2836-2843页

作者： er Hong Xi-er Chen Jia Mao Jing-Jing Zhou ling Chen Jia-Yi Xu Wei Tao Department of Respiratory Ningbo Municipal Hospital of Traditional Chinese MedicineAffiliated to Zhejiang Chinese Medical UniversityNingbo 315000Zhejiang ProvinceChina Department of Radiology Ningbo Municipal Hospital of Traditional Chinese MedicineAffiliated to Zhejiang Chinese Medical UniversityNingbo 315000Zhejiang ProvinceChina

BACKGROUND The emergence of secondary drug resistance when treating epidermal growth factor receptor(EGFR)mutated non-small cell lung cancer(NSCLC)using EGFRtyrosine kinase inhibitors(EGFR-TKIs),seriously affects the therapeutic efficacy and survival of ***,we report a case of advanced NSCLC focusing on the application of multiple biopsy modalities to reveal the development of multiple resistance mechanisms during targeted *** SUMMARY A 54-year-old male patient presented with EGFR 19Del-mutated advanced lung adenocarcinoma,and exhibited the development of a T790M mutation during initial TKI *** 3 mo of Osimertinib treatment,a mixed response was *** biopsy of the progressive lesion showed transformation to small cell lung cancer(SCLC)harboring RB1 and TP53 mutations,with loss of the original T790M mutation.A standard chemotherapy regimen with Anlotinib for SCLC was *** biopsy revealed adenocarcinoma combined with SCLC after tumor *** patient’s overall survival was 24 *** Multiple biopsy modalities can reveal the development of multiple resistance mechanisms which help with treatment *** treatment regimens according to the drug resistance mechanism significantly improved the prognosis of such patients.

关键词： Adenocarcinoma Epidermal growth factor receptor-tyrosine kinase inhibitor Epidermal growth factor receptor-T790M mutation Small cell lung cancer transformation Chemotherapy Case report