检索结果-南通市图书馆

Preventive effects of cannabis on neurotoxic and hepatotoxic activities of malathion in rat

Asian Pacific Journal of Tropical Medicine 2018年第4期11卷 272-279页

作者： Omar M.E.Abdel-Salam Amany A Sleem eman R youness Fatma A.Morsy Department of Toxicology and Narcotics National Research Centre Department of Pharmacology National Research Centre Department of Medical Biochemistry National Research Centre Department of Pathology National Research Centre

Objective: To investigate the effect of Cannabis sativa extract on the development of neuroand hepato-toxicity caused by malathion injection in rats. Methods: The extract of Cannabis sativa was obtained from the plant resin by chloroform treatment. Δ~9-Tetrahydrocannabinol content of the extract(20%) was quantified using gas chromatography–mass spectrometry. The doses of cannabis extract were expressed as Δ~9-tetrahydrocannabinol content of 10 or 20 mg/kg. Malathion(150 mg/kg) was intraperitoneally administered followed after 30 min by the cannabis extract(10 or 20 mg/kg, subcutaneously). Rats were euthanized 4 h later. Malondialdehyde(MDA), reduced glutathione(GSH), nitric oxide and paraoxonase-1(PON-1) activity were determined in brain and liver. Brain 5-lipoxygenase and butyrylcholinesterase(BChE) activity were measured as well. Histopathological examination of brain and liver tissue was also performed. Results: Compared to controls, malathion resulted in increased oxidative stress in brain and liver. MDA and nitric oxide concentrations were significantly increased(P<0.05) and GSH significantly decreased with respect to control levels(P<0.05). Malathion also significantly inhibited PON-1 and BChE activities but had no effect on brain 5-lipoxygenase. Brain MDA concentrations were not altered by cannabis treatment. Cannabis at 20 mg/kg, however, caused significant increase in nitric oxide and restored the GSH and PON-1 activity. Brain BChE activity significantly decreased by 26.1%(P<0.05) after treatment with 10 mg/kg cannabis. Cannabis showed no effect on brain 5-lipoxygenase. On the other hand, rats treated with cannabis exhibited significantly higher levels of liver MDA, nitric oxide and PON-1 activity compared with the malathion control group. Rats treated with only malathion exhibited spongiform changes, neuronal damage in the cerebral cortex and degeneration of some Purkinje cells in the cerebellum. There were also hepatic vacuolar degeneration and dilated an

关键词： Cannabis sativa Malathion Liver damage Brain damage Oxidative stress Paraoxonase 5-Lipoxygenase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Association of vitamin D receptor gene polymorphism(VDR)with vitamin D deficiency,metabolic and inflammatory markers in Egyptian obese women

引用

Genes & Diseases 2017年第3期4卷 176-182页

作者： Moushira Zaki Sanaa Kamal Walaa ABasha eman youness Wafaa Ezzat Hala El-Bassyouni Khalda Amr Biological Anthropology Department National Research CentreGizaEgypt Medical Biochemistry Department National Research CentreGizaEgypt Internal Medicine Department National Research CentreGizaEgypt Clinical Genetics Department National Research CentreCairoEgypt Medical Molecular Genetics Department National Research CentreEgypt

Vitamin D deficiency might contribute to the pathogenesis of metabolic syndrome and could cause immune *** aim of this study is to analyze the associations between Vitamin D receptor(VDR)gene polymorphism,serum 25-hydroxy vitamin D,metabolic and inflammatory biomarkers in Egyptian obese *** study included 201 obese women with vitamin D deficiency and 249 obese matched age healthy controls with sufficient vitamin D *** age ranged between 25 and 35 *** biomarkers(interleukin-6 and C-reactive protein)and serum 25(OH)D were measured by enzyme-linked immunosorbent *** resistance(IR)was determined by the homeostasis model assessment of insulin resistance(HOMA-IR).Vitamin D receptor(VDR)gene polymorphisms of FokI,ApaI,and TaqI were studied by PCR using the restriction fragment length polymorphism(RFLP)*** women with vitamin D deficiency had significant higher values of inflammatory and metabolic parameters compared to ***-logistic regression showed associations between 25(OH)D deficiency and metabolic components when comparing cases with ***,cases carrying polymorphic alleles showed significant lower levels of serum 25(OH)D and higher HOMA-IR,blood pressure levels and lipid parameters compared to those with the wild type homozygote in obese cases with vitamin D *** D deficiency in Egyptian obese women with vitamin D deficiency is associated with abnormal metabolic components and abnormal inflammatory ***,VDR polymorphisms play important role in immune and inflammation status.

关键词： Inflammatory biomarkers Obese women Vitamin D deficiency Vitamin D receptor(VDR)gene polymorphism Egyptian

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acetylcholinesterase,butyrylcholinesterase and paraoxonase 1 activities in rats treated with cannabis,tramadol or both

引用

Asian Pacific Journal of Tropical Medicine 2016年第11期9卷 1066-1071页

作者： Omar M.E.Abdel-Salam eman R.youness Yasser A.Khadrawy Amany A.Sleem Department of Toxicology and Narcotics National Research Centre Cairo Department of Medical Biochemistry National Research Centre Cairo Department of Physiology National Research Centre Cairo Department of Pharmacology National Research Centre Cairo

Objective:To investigate the effect of Cannabis sativa resin and/or tramadol,two commonly drugs of abuse on acetylcholinesterase and butyrylcholinesterase activities as a possible cholinergic biomarkers of neurotoxicity induced by these ***:rats were treated with cannabis resin(5,10 or 20 mg/kg)(equivalent to the active constituent A'-tetrahydrocannabinol),tramadol(5,10 and 20 mg/kg) or tramadol(10 mg/kg)combined with cannabis resin(5,10 and 20 mg/kg) subcutaneously daily for 6 ***(AChE) and butyrylcholinesterase(BChE) activities were measured in brain and *** also measured the activity of paraoxonase-1(PONl) in serum of rats treated with these ***:(i) AChE activity in brain increased after 10-20 mg/kg cannabis resin(by 16.3%-36.5%).AChE activity in brain did not change after treatment with 5-20 mg/kg *** administration of both cannabis resin(5,10 or 20 mg/kg) and tramadol(10 mg/kg) resulted in decreased brain AChE activity by 14.1%,12.9%and 13.6%,respectively;(ii) BChE activity in serum was markedly and dose-dependenlly inhibited by cannabis resin(by 60.9%-76.9%).BChE activity also decreased by 17.6%-36.5%by 10-20mg/kg tramadol and by 57.2%-63.9%by the cannabis resin/tramadol combined treatment;(iii)Cannabis resin at dose of 20 mg/kg increased serum PONl activity by 25.7%.In contrast,tramadol given at 5,10 and 20 mg/kg resulted in a dose-dependent decrease in serum PON1 activity by 19%,36.7%,and 46.1%,***,treatment with cannabis resin plus tramadol resulted in 40.2%,35.8%,30.7%inhibition of PONl activity compared to the saline ***:these data suggest that cannabis resin exerts different effects on AChE and BChE activities which could contribute to the memory problems and the decline in cognitive function in chronic users.

关键词： Cannabis sativa Tramadol Cholinesterases Memory Cognitive decline

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain

引用

Asian Pacific Journal of Tropical Medicine 2018年第1期11卷 40-47页

作者： Omar M.E.Abdel-Salam Amany A Sleem eman R youness Nadia A Mohammed Enayat A Omara Department of Toxicology and Narcotics National Research Centre Department of Pharmacology National Research Centre Department of Medical Biochemistry National Research Centre Department of Pathology National Research Centre

Objective: To investigate the effect of the prostaglandin E1 analogue misoprostol on oxidative stress and neurodegeration caused by subcutaneous rotenone administration in rats. Methods:Rotenone was administered in a dose of 1.5 mg/kg every other day for 2 weeks. Starting from the 1 st day of rotenone injection, rats were subcutaneously treated with misoprostol at doses of10, 100 or 1 000 μg/kg. Rats were evaluated for brain lipid peroxidation(malondialdehyde:MDA), reduced glutathione(GSH), nitric oxide(NO) levels, and paraoxonase-1(PON-1) *** concentrations of the anti-apoptotic protein B cell/lymphoma-2(Bcl-2) were determined in the striatum. Histopathologic examination and the expression of inducible nitric oxide synthase(iNOS) in the cerebral cortex and striatum were also performed. Results: Compared with the vehicle-treated group, rotenone caused a significant increase in brain lipid proxidation(MDA)by 61%(P<0.05) accompanied by an increase in NO by 73.1%(P<0.05) and a decrease in GSH concentration by 29.4%(P<0.05). In addition, brain PON-1 activity significantly decreased by63.0%(P<0.05) and striatal Bcl-2 significantly decreased by 27.9%(P<0.05) with respect to the corresponding control value. Brain sections from rotenone treated rats showed extensive dark pyknotic and apoptotic nuclei in neurons, shrunken cytoplasm and perineuronal *** also caused pronounced expression of iNOS in the cerebral cortex and *** with misoprostol at doses of 100 and 1 000 μg/kg resulted in decreased brain MDA(by 16.5%-23.0%)(P<0.05) and NO levels(by 37.1%-40.7%)(P<0.05) and increased GSH concentrations(by 18.8%-30.1%)(P<0.05). PON-1 activity was significantly increased by80.0%-114.8%(P<0.05) by misoprostol at 100 and 1 000 μg/kg, respectively. In addition,misoprostol treatment restored striatal Bcl-2 concentrations to its normal value. Misoprostol treatment resulted in markedly reduced brain injury and decreased iNOS expression in the cereb

关键词： Misoprostol Rotenone Brain oxidative stress B cell/lymphoma-2 Paraoxonase

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Serum homocysteine,lipid profile and BMI as atherosclerotic risk factors in children with numerical chromosomal aberrations

引用

World Journal of Pediatrics 2022年第6期18卷 443-448页

作者： eman R.youness Sherien MEl-Daly Hanaa Reyad Abdallah Hala T.El-Bassyouni Hisham Megahed Azzah A.Khedr Marwa Elhady Walaa Alsharany Abuelhamd Medical Biochemistry Department Medical Research DivisionNational Research CentreGizaEgypt Biological Anthropology Department Medical Research DivisionNational Research CentreGizaEgypt Clinical Genetics Department Human Genetics and Genome Research DivisionNational Research CentreEl-Tahrir StreetPO:12622GizaEgypt Human Cytogenetics Department Human Genetics and Genome Research DivisionNational Research CentreGizaEgypt Pediatric Department Faculty of Medicine for GirlsAl-Azhar UniversityCairoEgypt Department of Pediatrics Faculty of MedicineCairo UniversityCairoEgypt

Down syndrome,Turner syndrome,and Klinefelter syndrome are the most common chromosomal disorders *** syndrome is caused by an extra copy of the 21st chromosome and has an incidence of 1 in 800 to 1000 live *** syndrom... 详细信息

关键词： chromosomal Turner Serum

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bougainvillea spectabilis flowers extract protects against the rotenone-induced toxicity

引用

Asian Pacific Journal of Tropical Medicine 2017年第5期10卷 457-466页

作者： Omar M.E.Abdel-Salam eman R.youness Nadia A.Ahmed Sayed A.El-Toumy Ahmed M.A.Souleman Nermeen Shaffie Dalia M.Abouelfadl Department of Toxicology and Narcotics National Research CentreCairoEgypt Department of Medical Biochemistry National Research CentreCairoEgypt Department of Chemistry of Tannins National Research CentreCairoEgypt Department of Phytochemistry and Plant Systematic National Research CentreCairoEgypt Department of Pathology National Research CentreCairoEgypt

Objective:To investigate the effect of two extracts of Bougainvillea spectabilis(B. spectabilis) flowers with yellow and pink/purple on brain oxidative stress and neuronal damage caused in rats by systemic rotenone injection. Methods:Rotenone 1.5 mg/kg was given three times per week alone or in combination with B. spectabilis flowers extracts(25 mg or 50 mg) via the subcutaneous route for 2 weeks. Brain concentrations of the lipid peroxidation marker malondialdehyde(MDA),reduced glutathione,nitric oxide(nitrite),the pro-inflammatory cytokine interleukin-1beta(Il-1β) as well as butyrylcholinesterase,and paraoxonase-1(PON-1) activities,were determined. Histopathology and caspase-3 immunohistochemistry were also performed. Results:Rotenone resulted in significant increases of brain MDA(the product of lipid peroxidation),and nitric oxide content along with decreased brain reduced glutathione. There were also marked and significant inhibition of brain PON-1 and BCh E activities and increased Il-1β in brain of rotenone-treated rats. B. spectabilis flowers extract itself resulted in brain oxidative stress increasing both lipid peroxidation and nitrite content whilst inhibiting PON-1 activity. The yellow flowers extract inhibited BCh E activity and increased brain Il-1β. When given to rotenone-treated rats,B. spectabilis extracts,however,decreased lipid peroxidation while their low administered doses increased brain GSH. Brain nitrite decreased by the pink extract but showed further increase by the yellow extract. Either extract,however,caused further inhibition of PON-1 activity while the yellow extract resulted in further inhibition of BChE activity. Histopathological studies indicated that both extracts protected against brain,liver and kidney damage caused by the toxicant. Conclusions:These data indicate that B. spectabilis flowers extracts exert protective effect against the toxic effects of rotenone on brain,liver and kidney. B. spectabilis flowers extracts decreased

关键词： Bougainvillea spectabilis Flowers Extract Rotenone Oxidative stress Parkinson’s disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Novel neuroprotective and hepatoprorective effects of citric acid in acute malathion intoxication

引用

Asian Pacific Journal of Tropical Medicine 2016年第12期9卷 1157-1169页

作者： Omar M.E.Abdel-Salam eman R.youness Nadia A.Mohammed Noha N.Yassen Yasser A.Khadrawy Safinaz Ebrahim El-Toukhy Amany A.Sleem Department of Toxicology and Narcotics National Research Centre Medical Biochemistry National Research Centre Pathology National Research Centre Physiology National Research Centre Pharmacology National Research Centre

Objective: To study the effect of citric acid given alone or combined with atropine on brain oxidative stress, neuronal injury, liver damage, and DNA damage of peripheral blood lymphocytes induced in the rat by acute malathion exposure. Methods: Rats were received intraperitoneal(i.p.) injection of malathion 150 mg/kg along with citric acid(200 or 400 mg/kg, orally), atropine(1 mg/kg, i.p.) or citric acid 200 mg/kg+atropine 1 mg/kg and euthanized 4 h later. Results: Malathion resulted in increased lipid peroxidation(malondialdehyde) and nitric oxide concentrations accompanied with a decrease in brain reduced glutathione, glutathione peroxidase(GPx) activity, total antioxidant capacity(TAC) and glucose concentrations. Paraoxonase-1, acetylcholinesterase(ACh E) and butyrylcholinesterase activities decreased in brain as well. Liver aspartate aminotransferase and alanine aminotransferase activities were raised. The Comet assay showed increased DNA damage of peripheral blood lymphocytes. Histological damage and increased expression of inducible nitric oxide synthase(i NOS) were observed in brain and liver. Citric acid resulted in decreased brain lipid peroxidation and nitric oxide. Meanwhile, glutathione, GPx activity, TAC capacity and brain glucose level increased. Brain ACh E increased but PON1 and butyrylcholinesterase activities decreased by citric acid. Liver enzymes, the percentage of damaged blood lymphocytes, histopathological alterations and i NOS expression in brain and liver was decreased by citric acid. Meanwhile, rats treated with atropine showed decreased brain MDA, nitrite but increased GPx activity, TAC, ACh E and glucose. The drug also decreased DNA damage of peripheral blood lymphocytes, histopathological alterations and i NOS expression in brain and liver. Conclusions: The study demonstrates a beneficial effect for citric acid upon brain oxidative stress, neuronal injury, liver and DNA damage due to acute malathion exposure.

关键词： Citric acid Malathion Oxidative stress Paraoxonase 1 Cholinesterase Comet assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The antioxidant status of the plasma in patients with breast cancer undergoing chemotherapy

引用

Open Journal of Molecular and Integrative Physiology 2011年第3期1卷 29-35页

作者： Omar M. E. Abdel-Salam eman R. youness Hafez F. Hafez 不详

The aim of the present study was to investigate the status of oxidative stress in the serum of patients affected with cancer breast. Changes in the levels of total antioxidant capacity (TAC), uric acid, malondialdehyde (MDA), nitric oxide (nitrite/nitrate), cupper and iron were measured in serum of patients affected by non-metastatic as well as metastatic cancer breast. Significant decrease in TAC (32.7-37.5%), uric acid (28.1%-49.2), MDA (20.7%-25.2%) and nitric oxide (50.4%-61.9) were found in both groups of cancer breast patients compared to the control group. Serum Cu++ concentrations were significantly lower in metastatic cancer patient group compared with both control and non-metastatic cancer groups. Fe++ in serum was significantly lower in patients with non-metastatic cancer compared to normal subjects and patients with metastatic cancer. Significant differences were also observed between patients with non-metastatic and metastatic cancer breast as regards serum uric acid, nitric oxide were higher were observed in non-metastatic compared with metastatic cancer breast.

关键词： Cancer Breast Chemotherapy Oxidative Stress Copper Iron

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nuclear Factor-Kappa B and Other Oxidative Stress Biomarkers in Serum of Autistic Children

引用

Open Journal of Molecular and Integrative Physiology 2015年第1期5卷 18-27页

作者： Omar M. E. Abdel-Salam eman R. youness Nadia A. Mohammed Walaa A. Abu Elhamed Department of Toxicology and Narcotics National Research Centre Cairo Egypt Department of Medical Biochemistry National Research Centre Cairo Egypt Department of Pediatrics Faculty of Medicine Cairo University Cairo Egypt

The aim of the present study was to investigate the status of oxidative stress in the serum of children affected with autism spectrum disorder. Twenty autistic children aged 3 to 12 years, were gender and age-matched with 20 typically developing children. Changes in the levels of the redox-sensing transcription factor nuclear factor-kappa B (NF-κB) was measured in serum of autistic children and controls. Other oxidative stress biomarkers such as malondialdehyde, reduced glutathione, total antioxidant capacity, catalase activity, and paraoxonase 1 activity were determined in serum as well. Significant increase was observed in serum NF-κB of autistic children compared to that in controls (by 138.6%). There was also marked increase in malondialdehyde level by 87.3% in autistic patients. Meanwhile, there were significant decreases in reduced glutathione (by 24%), catalase activity (by 40.8%), paraoxonase 1 activity (by 36.6%), and total antioxidant capacity (by 36.5%) compared to the control group. These data clearly demonstrate increased oxidative stress in serum of autistic children and suggest that the NF-κB signaling pathway is activated in autism, possibly due to increased oxidative burden.

关键词： Autism Spectrum Disorder Oxidative Stress Redox-Sensing Transcription Factor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：