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Effect of Compost Based Substrate and Mycorrhizal Inoculum in Potted Geranium Plants

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Journal of Life Sciences 2012年第7期6卷 771-775页

作者： Monica Tullio Federico Calviello elvira Rea CRA Agricultural Research Council Research Centre for the Soil-Plant System Via Della Navicella 2-4 Rome 00184 Italy

Two are the critical factors in the commercial production of ornamental plants： the substrate and the fertilization method, since the ＂sustainable floriculture＂ discourages the use of synthetic chemical fertilizers and peat-based substrates. The aim of this paper was to test a peat/compost based substrate and a guano ＋ mycorrhizal inoculum fertilizer mixture, in order to obtain a sustainable quality yield of geranium plants. Geranium cuttings （Pelargonium zonale cv. real polaris and Pelargonium grandiflorum cv. lotus） were grown for two months in a glasshouse. Peat, in the growing substrate, was replaced with high quality compost （20% and 40% as reduction percentages）. The fertilizer mixture was made up of guano （3 g/L） and mycorrhizal inoculum （7.5 L/m^3）. Plants of Pelargonium cv. Real polaris, grown on a substrate made up of peat and 20% of high quality compost, presented the better trade features, so to satisfy the ＂sustainable floriculture＂ need.

关键词： Compost-peat horticultural products pelargonium guano mycorrhizal inoculums.

Regulation of Genetically Modified Food Use in the Russian Federation

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Food and Nutrition Sciences 2016年第9期7卷 743-751页

作者： Nadezhda V. Tyshko elvira O. Sadykova Federal State Budgetary Scientific Institution “Federal Research Centre of Nutrition Biotechnology and Food Safety” Moscow Russian Federation

Practical application of new methods of а plant genome transformation resulted in the need for strict regulation of the safety assessment process of genetically modified organisms of plant origin, intended for use in food. The devise of Genetically Modified Organisms (GMO) of plant origin safety assessment system, which is currently valid in the Russian Federation, was initiated in 1995-1996. Not only does the system accumulate all domestic and foreign experience, it also includes the latest scientific approaches, based on achievements of fundamental science. In the present article authors focused on issues of the basic legislative regulations of plant biotechnology in the Russian Federation, and approaches to the safety assessment of GM food and control over such food. GMO safety assessment is carried out at the stage of State registration. The subject of registration is novel food products derived from GMO, manufactured in the Russian Federation, as well as food products, entering the Russian Federation for the first time. In the system of medical and biological assessment of the GMO safety, along with general toxicological research, the study of specific types of toxicity in in vivo experiments takes a prominent place. In accordance with established research practice, which uses an integrated approach, and provides with the most complete and reliable information on potential reprotoxic, genotoxic, immunotoxic and allergenic effects of GMO, as well as enables to reveal possible unintended effects of genetic modification. From 1999 to 2016 22 GM lines passed the entire cycle of medical-biological investigations. By 2016 there had been amassed scientific foundation on GMO safety, which includes the analysis of result of research, carried out within the framework of GMO state registration in the Russian Federation, as well as domestic and international data, presented by scientific literature on the problem of biotechnological food safety, at both registration stage

关键词： Plant Biotechnology GM Food GMO Safety Assessment GMO Legislative Regulation GM Food Labelling Control over GMO

Potential of Lunasin Orally-Administered in Comparison to Intraperitoneal Injection to Inhibit Colon Cancer Metastasis <i>in Vivo</i>

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Journal of Cancer Therapy 2013年第6期4卷 34-43页

作者： Vermont P. Dia elvira Gonzalez de Mejia Department of Food Science and Human Nutrition University of Illinois at Urbana-Champaign Urbana USA

Lunasin is a bioactive peptide originally isolated from soybean and has demonstrated chemopreventive and anti-cancer properties against skin, colon and breast cancers. The objective of the study was to evaluate the capability of intraperitoneally and orally-administered soybean-derived peptide lunasin to inhibit KM12L4 human colon cancer cell metastasis in a mouse model. Intraperitoneal (i.p.) injection of lunasin (4 mg/kg bw/day) reduced the number of liver metastasis by 50% (P = 0.047) and the liver weight/body weight ratio by 23% (P = 0.039). Oral administration of lunasin reduced the number of liver metastasis by 56% (8 mg/kg bw/day, P = 0.293) and 94% (20 mg/kg bw/day, P = 0.247). Immunohistochemical staining of the liver-tissue section showed that lunasin at 20 mg/kg bw dose did not significantly reduce the expression of proliferating cell nuclear antigen, increased the expression of proapoptotic Bax by 2.7-fold but also increased the expression of the antiapoptotic Bcl-2 by 3.8-fold. Regarding epigenetic markers, H3K18 was not significantly affected by either oral dose while H4K8 was dose dependently increased by 2.3-fold (P = 0.001, 8 mg/kg bw) and 2.7-fold (P s of administration used leading to digestion of lunasin when given by oral gavage. In conclusion, lunasin reduced colon cancer metastasis in vivo;however, more studies are needed to determine the oral dose of lunasin and prevent colon cancer metastasis.

关键词： Lunasin Colon Cancer Metastasis Apoptosis Histones

Prevalence and clinical significance of antiphospholipid antibodies among hospitalized COVID-19 patients

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Asian Pacific Journal of Tropical Medicine 2021年第8期14卷 350-355页

作者： Cesarius Singgih Wahono Hani Susianti Tri Wahyudi Iman Dantara Perdana Aditya Rahman Mirza Zaka Pratama Indah Adhita Wulanda Khoirunisah Dwi Hartanti elvira Sari Dewi Kusworini Handono Rheumatology and Immunology Division Department of Internal MedicineFaculty of MedicineUniversity of BrawijayaIndonesia Department of Clinical Pathology Faculty of MedicineUniversity of BrawijayaIndonesia Basic Nursing Department Faculty of MedicineUniversity of BrawijayaIndonesia

Objective:To describe the prevalence of antiphospholipid antibodies in coronavirus disease-19(COVID-19)and to find potential associations between antiphospholipid antibody positivity and clinical ***:From September to November 2020,clinical and laboratory data were collected from 50 COVID-19 patients hospitalized at Saiful Anwar General Hospital in Malang,*** antibodies were measured by finding Ig M anti-β2 glycoprotein,lupus anticoagulant,and Ig M/Ig G *** characteristics,thrombotic events,ICU admission,and mortality during hospitalization were *** severity was defined by the Guidelines for the Prevention and Control of COVID-19,***:Among 50 patients,5 patients(10.0%)were positive for antiphospholipid antibodies:4 patients(80.0%)had Ig M anti-β2 glycoprotein and 1 patient had Ig G anti-cardiolipin(20.0%)and Ig M anti-cardiolipin(20.0%),none of lupus anticoagulant was *** antibodies were associated with anosmia(OR 8.1;95%CI 1.1-57.9;P=0.018),nausea and vomiting(OR 12.4;95%CI 1.2-122.6;P=0.010),diarrhea(OR 9.8;95%CI 1.3-70.9;P=0.010),cardiovascular disease(OR 1.4;95%CI 1.0-1.9;P=0.001),chronic kidney disease(OR 12.0;95%CI 1.6-90.1;P=0.05),acute coronary syndrome(OR 29.3;95%CI 2.0-423.7;P=0.001),moderate(OR 0.11;95%CI 0.01-1.10;P=0.031)and severe(OR 18.5;95%CI 1.8-188.4;P=0.002)disease severity,and in-hospital mortality(OR 8.1;95%CI 1.1-57.9;P=0.018).However,there is no correlation between the presence of antiphospholipid antibody and ICU ***:In summary,the prevalence of antiphospholipid antibodies in COVID-19 patients is low,mainly against Ig M anticardiolipin,and is associated with an acute coronary syndrome,gastrointestinal manifestations,moderate and severe disease severity,and increased risk of mortality.

关键词： Antiphospholipid antibodies COVID-19 COVID-19 disease severity Mortality

Prozessorientiertes Qualit?tsmanagement im Dienstleistungsbereich übersetzen

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Current surgical treatment of diverticular disease in the Netherlands

2006年

作者： Mertin elvira.

来源：畅想之星图书光盘版（南... 评论

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World Journal of Gastroenterology 2010年第14期16卷 1742-1746页

作者： Annelien N Morks Bastiaan R Klarenbeek elvira R Flikweert Donald L van der Peet Thomas M Karsten Eric H Eddes Miguel A Cuesta Peter W de Graaf Department of Surgery Reinier de Graaf Gasthuis PO Box 5011 2600 GA Delft The Netherlands Department of Surgery VU University Medical Center PO Box 7057 1007 MB Amsterdam The Netherlands Department of Surgery Deventer Ziekenhuis PO Box 5001 7400 GC Deventer The Netherlands

AIM: To evaluate the development of diagnostic tools, indications for surgery and treatment modalities concerning diverticular disease (DD) in the Netherlands. METHODS: Data were collected from 100 patients who underwent surgery for DD in three Dutch hospitals. All hospitals used the same standardized data base. The collected data included patient demographics, patient history, type of surgery and complications. Patients were divided into two groups, one undergoing elective surgery (elective group) and the other undergoing acute surgery (acute group). RESULTS: Two hundred and ninety-nine patients were admitted between 2000 and 2007. One hundred and seventy-eight patients underwent acute surgery and 121 patients received elective operations. The median age of the 121 patients was 69 years (range: 28-94 years), significantly higher in acute patients (P = 0.010). Laparoscopic resection was performed in 31% of elective patients. In the acute setting, 61% underwent a Hartmann procedure. The overall morbidity and mortality were 51% and 10%, and 60% and 16% in the acute group, which were significantly higher than in the elective group (36% and 1%). Only 35% of the temporary ostomies were restored. CONCLUSION: This study gives a picture of current surgical practice for DD in the Netherlands. New developments are implemented in daily practice, resulting in acceptable morbidity and mortality rates.

关键词： Diverticulitis Surgery Diverticular disease

Systemic epigallocatechin gallate protects against retinal degeneration and hepatic oxidative stress in the P23H-1 rat

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Neural Regeneration Research 2022年第3期17卷 625-631页

作者： Lorena Perdices Lorena Fuentes-Broto Francisco Segura Ana Cavero elvira Orduna-Hospital Gema Insa-Sánchez Ana Isabel Sánchez-Cano Laura Fernández-Sánchez Nicolás Cuenca Isabel Pinilla Aragón Institute for Health Research(IIS Aragón) ZaragozaSpain Pharmacology Physiology&Legal and Forensic MedicineUniversity of ZaragozaZaragozaSpain Department of Applied Physics University of ZaragozaZaragozaSpain Health Sciences Faculty San Jorge UniversityVillanueva de GállegoSpain Department of Ophthalmology Miguel Servet University HospitalZaragozaSpain Department of Physiology Genetics and MicrobiologyUniversity of AlicanteAlicanteSpain Department of Surgery Gynecology and ObstetricsUniversity of ZaragozaZaragozaSpain/Department of OphthalmologyLozano Blesa University HospitalZaragozaSpain

Retinitis pigmentosa(RP) is a group of inherited retinal disorders that lead to photoreceptor *** has been reported to be related to oxidative stress,autophagy,and inflammation.(-)-Epigallocatechin gallate(EGCG),the most abundant catechin-based flavonoid in green tea leaves,has significant antioxidant,anti-carcinogenic,antimicrobial,and neuroprotective ***,given its low molecular weight and hydrophilic properties,can cross the blood-retinal barrier and is able to reach different ocular tissues such as the lens,cornea,and *** has been shown to provide retinal protection against ischemia;sodium nitroprusside-,N-methyl-D-aspartate-,lipopolysaccharide-,light-,sodium iodate-,or H2 O2-induced damage and diabetic *** suggests that systemic EGCG administration has the potential to protect against retinal degenerative or neurodegenerative diseases such as *** aim of this work was to investigate whether EGCG can protect against RP progression in the animal P23 H line 1,the model of *** P23 H rats were crossed with pigmented Long Evans rats to produce offspring exhibiting the clinical features of *** P23 H rats were treated via intraperitoneal injection with saline or EGCG at a dose of 25 mg/kg every week from P100 to P160 and then compared to wild-type Long Evans *** treated with EGCG showed better visual and retinal electrical function with increased contrast sensitivity and b-wave values compared with those observed in P23 H rats treated with *** reduced lipid peroxidation and increased total antioxidant capacity and catalase and superoxide dismutase *** differences were observed in visual acuity,nitrate levels,nitrite levels or glutathione S-transferase *** conclusion,EGCG not only reduced the loss of visual function in P23 H rats but also improved the levels of antioxidant enzymes and reduced oxidative *** study was approved by the Institutional Animal Care and Use Committee(CE

关键词： antioxidants contrast sensitivity electroretinogram green tea neurodegeneration oxidative stress retinal degeneration retinitis pigmentosa systemic visual acuity

Lovastatin as a supplement to mitigate rumen methanogenesis:an overview

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Journal of Animal Science and Biotechnology 2022年第2期13卷 353-364页

作者： Amaury Ábrego-Gacía Héctor M.Poggi-Varaldo Vania Robles-González Teresa Ponce-Noyola Graciano Calva-Calva elvira Ríos-Leal Daniel Estrada-Bárcenas Alfredo Mendoza-Vargas Department of Biotechnology and Bioengineering CINVESTAV-IPNP.O.Box 17-74007000 Mexico CityMexico Environmental Biotechnology and Renewable Energies Group CINVESTAV-IPNP.O.Box 17-74007000 Mexico CityMexico Instituto de Hidrología Universidad Tecnológica de la MixtecaOaxaca 69000 Huajuapan de LeónMexico National Collection of Microbial and Cellular Cultures CINVESTAV-IPNP.O.Box17-74007000 Mexico CityMexico Unidad de Secuenciación e Identificación de Polimorfismos Instituto Nacional de Medicina Genómica14610 Mexico CityMexico

Methane from enteric fermentation is the gas with the greatest environmental impact emitted by ***(Lv)addition to feedstocks could be a strategy to mitigate rumen methane emissions via decreasing the population of methanogenic archaea(MA).Thus,this paper provides the first overview of the effects of Lv supplementation,focusing on the inhibition of methane production,rumen microbiota,and ruminal *** indicated that Lv treatment had a strong anti-methanogenic effect on pure strains of ***,there are uncertainties from in vitro rumen fermentation trials with complex substrates and rumen ***-state fermentation(SSF)has emerged as a cost-effective option to produce *** this way,SSF of agricultural residues as an Lv-carrier supplement in sheep and goats demonstrated a consistent decrease in ruminal methane *** experimental evidence for in vitro conditions showed that Lv did not affect the volatile fatty acids(VFA).However,in vivo experiments demonstrated that the production of VFA was *** did not negatively affect the digestibility of dry matter during in vitro and in vivo methods,and there is even evidence that it can induce an increase in *** the rumen microbiota,populations of MA were reduced,and no differences were detected in alpha and beta diversity associated with Lv ***,some changes in the relative abundance of the microbiota were *** studies are recommended on:(i)Lv biodegradation products and stability,as well as its adsorption onto the solid matter in the rumen,to gain more insight on the“available”or effective Lv concentration;and(ii)to determine whether the effect of Lv on ruminal fermentation also depends on the feed composition and different ruminants.

关键词： Fermentation Lovastatin Methanogenesis Microbiota Rumen

Current knowledge on the laboratory diagnosis of Clostridium difficile infection

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World Journal of Gastroenterology 2017年第9期23卷 1552-1567页

作者： Adrián Martínez-Meléndez Adrián Camacho-Ortiz Rayo Morfin-Otero Héctor Jesús Maldonado-Garza Licet Villarreal-Trevino elvira Garza-González Departamento de Microbiología e Inmunología Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo León.Pedro de Alba S/NCiudad UniversitariaSan Nicolás de los Garza Servicio de Infectología Hospital Universitario“Dr.JoséEleuterio González”Universidad Autónoma de Nuevo León.Av.Francisco I.Madero Pte.S/NCol.Mitras Centro.Monterrey Hospital Civil de Guadalajara “Fray Antonio Alcalde”e Instituto de Patología Infecciosa y ExperimentalCentro Universitario de Ciencias de la SaludUniversidadde Guadalajara.Sierra Mojada 950Col.Independencia Servicio de Gastroenterología Hospital Universitario“Dr.JoséEleuterio González”Universidad Autónoma de Nuevo León.Av.Francisco I.Madero Pte.S/NCol.Mitras Centro.Monterrey

Clostridium difficile (C. difficile) is a spore-forming, toxin-producing, gram-positive anaerobic bacterium that is the principal etiologic agent of antibiotic-associated diarrhea. Infection with C. difficile (CDI) is characterized by diarrhea in clinical syndromes that vary from selflimited to mild or severe. Since its initial recognition as the causative agent of pseudomembranous colitis, C. difficile has spread around the world. CDI is one of the most common healthcare-associated infections and a significant cause of morbidity and mortality among older adult hospitalized patients. Due to extensive antibiotic usage, the number of CDIs has increased. Diagnosis of CDI is often difficult and has a substantial impact on the management of patients with the disease, mainly with regards to antibiotic management. The diagnosis of CDI is primarily based on the clinical signs and symptoms and is only confirmed by laboratory testing. Despite the high burden of CDI and the increasing interest in the disease, episodes of CDI are often misdiagnosed. The reasons for misdiagnosis are the lack of clinical suspicion or the use of inappropriate tests. The proper diagnosis of CDI reduces transmission, prevents inadequate or unnecessary treatments, and assures best antibiotic treatment. We review the options for the laboratory diagnosis of CDI within the settings of the most accepted guidelines for CDI diagnosis, treatment, and prevention of CDI.

关键词： Clostridium difficile Toxigenic culture Nucleic acid amplification tests Enzyme immunoassay Diagnosis Glutamate dehydrogenase